SSGCID: Target Page

StmaA.18897.a

Uridylate kinase (EC 2.7.4.22) (pyrH)

CENTER ID:	StmaA.18897.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.18897.a.B1.GE43552	Full length(StmaA.18897.a)		1	242

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.1441

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKLAYRRIL LKLSGEALMG DEDYGIDPKI INRLAREVIE AQQAGAEVAL VIGGGNIFRG AGLAAGGMDR VTGDQMGMLA TVINALAMQD ALEKLGAKAR VMSAIKINDV CEDYIRRRAI RHLEKGRLVI FAAGVGSPFF TTDSGAALRA IEIGADLLLK ATKVDGVYDK DPNKHSDAVR FDSLSYDEVI RRGLEVMDTA AFALARDSDL PMRVFDMGQP GELLKILNGE NIGTLVQGRD PA

NT Sequence

ATGTCCAAGC TCGCCTATCG CCGCATCCTT CTGAAACTGT CCGGGGAGGC GCTGATGGGA GATGAGGACT ACGGCATCGA CCCGAAGATC ATCAACCGCC TGGCCCGTGA GGTCATCGAA GCCCAGCAGG CCGGTGCCGA AGTGGCCCTG GTGATCGGCG GCGGCAACAT CTTCCGCGGC GCCGGCCTTG CCGCCGGCGG CATGGACCGG GTCACCGGCG ACCAGATGGG CATGCTGGCC ACGGTCATCA ACGCCCTGGC CATGCAGGAC GCCCTGGAGA AGCTGGGCGC CAAGGCGCGC GTGATGAGCG CGATCAAGAT CAACGACGTG TGCGAGGACT ACATCCGCCG CCGCGCCATC CGCCACCTGG AAAAGGGCCG CCTGGTGATC TTCGCCGCCG GCGTCGGCAG CCCGTTCTTC ACCACCGACT CCGGCGCCGC CCTGCGCGCG ATCGAGATCG GTGCCGACCT GCTGCTGAAG GCCACCAAGG TCGATGGCGT GTACGACAAG GACCCGAACA AGCACAGCGA TGCGGTCCGC TTCGACAGCC TGAGCTATGA CGAAGTGATC CGCCGCGGCC TGGAAGTGAT GGATACCGCC GCCTTCGCCC TGGCCCGCGA CAGCGACCTG CCGATGCGCG TGTTCGACAT GGGCCAGCCG GGCGAGCTGC TGAAGATCCT CAACGGCGAG AACATCGGCA CCCTGGTCCA GGGCCGCGAT CCGGCC

Details for StmaA.18897.a.B1.GE43552

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS KLAYRRILLK LSGEALMGDE DYGIDPKIIN RLAREVIEAQ QAGAEVALVI GGGNIFRGAG LAAGGMDRVT GDQMGMLATV INALAMQDAL EKLGAKARVM SAIKINDVCE DYIRRRAIRH LEKGRLVIFA AGVGSPFFTT DSGAALRAIE IGADLLLKAT KVDGVYDKDP NKHSDAVRFD SLSYDEVIRR GLEVMDTAAF ALARDSDLPM RVFDMGQPGE LLKILNGENI GTLVQGRDPA

Validated NT Sequence

atcttactca cttaggccgg atcgcggccc tggaccaggg tgccgatgtt ctcgccgttg aggatcttca gcagctcgcc cggctggccc atgtcgaaca cgcgcatcgg caggtcgctg tcgcgggcca gggcgaaggc ggcggtatcc atcacttcca ggccgcggcg gatcacttcg tcatagctca ggctgtcgaa gcggaccgca tcgctgtgct tgttcgggtc cttgtcgtac acgccatcga ccttggtggc cttcagcagc aggtcggcac cgatctcgat cgcgcgcagg gcggcgccgg agtcggtggt gaagaacggg ctgccgacgc cggcggcgaa gatcaccagg cggccctttt ccaggtggcg gatggcgcgg cggcggatgt agtcctcgca cacgtcgttg atcttgatcg cgctcatcac gcgcgccttg gcgcccagct tctccagggc gtcctgcatg gccagggcgt tgatgaccgt ggccagcatg cccatctggt cgccggtgac ccggtccatg ccgccggcgg caaggccggc gccgcggaag atgttgccgc cgccgatcac cagggccact tcggcaccgg cctgctgggc ttcgatgacc tcacgggcca ggcggttgat gatcttcggg tcgatgccgt agtcctcatc tcccatcagc gcctccccgg acagtttcag aaggatgcgg cgataggcga gcttggacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca agctcgccta tcgccgcatc cttctgaaac tgtccgggga ggcgctgatg ggagatgagg actacggcat cgacccgaag atcatcaacc gcctggcccg tgaggtcatc gaagcccagc aggccggtgc cgaagtggcc ctggtgatcg gcggcggcaa catcttccgc ggcgccggcc ttgccgccgg cggcatggac cgggtcaccg gcgaccagat gggcatgctg gccacggtca tcaacgccct ggccatgcag gacgccctgg agaagctggg cgccaaggcg cgcgtgatga gcgcgatcaa gatcaacgac gtgtgcgagg actacatccg ccgccgcgcc atccgccacc tggaaaaggg ccgcctggtg atcttcgccg ccggcgtcgg cagcccgttc ttcaccaccg actccggcgc cgccctgcgc gcgatcgaga tcggtgccga cctgctgctg aaggccacca aggtcgatgg cgtgtacgac aaggacccga acaagcacag cgatgcggtc cgcttcgaca gcctgagcta tgacgaagtg atccgccgcg gcctggaagt gatggatacc gccgccttcg ccctggcccg cgacagcgac ctgccgatgc gcgtgttcga catgggccag ccgggcgagc tgctgaagat cctcaacggc gagaacatcg gcaccctggt ccagggccgc gatccggcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat