SSGCID: Target Page

StmaA.18892.a

Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2) (accA)

CENTER ID:	StmaA.18892.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.18892.a.B1.GE43551	Full length(StmaA.18892.a)		1	319

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.1427

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNPNYLDFEQ PIADLEAKIQ ELRNASAGPA VNVEAEVHAL QDKLRMRTAQ IFRNLTSWQV LQLARHPSRP YTADYLRVMF DEFQELAGDR AFADDKAIMG GLARINGRAV MVIGHQKGRD TKEKIKRNFG MPKPEGYRKA LRLMKMAERF GLPVLTLIDT AGAWPGIDAE SRGQSEAIAR NLIEMAELKV PIICTVIGEG GSGGALALGV GDRTVMLEYA VYSTITPEGC ASILWKDAGK AKDAAEQLGL TAPRLKSLGL VDKVVREPTG GAHRNPTQMA KRLKAVLLNE LDALDKLSTE QLLEQRYARL RSYGTYEAA

NT Sequence

ATGAATCCGA ACTACCTCGA CTTCGAGCAA CCCATCGCCG ACCTGGAAGC CAAGATCCAG GAACTGCGCA ACGCCAGTGC CGGGCCGGCG GTCAATGTCG AGGCGGAAGT GCACGCGCTG CAGGACAAGC TGCGCATGCG CACCGCGCAG ATCTTCCGCA ACCTGACCTC GTGGCAGGTG CTGCAGCTGG CCCGTCATCC GTCGCGCCCG TACACCGCCG ACTACCTCCG CGTCATGTTC GATGAGTTCC AGGAGCTGGC CGGCGACCGT GCCTTCGCCG ACGACAAGGC GATCATGGGT GGCCTGGCCC GCATCAACGG CCGTGCAGTG ATGGTGATCG GCCACCAGAA GGGCCGTGAC ACCAAGGAGA AGATCAAGCG CAACTTCGGC ATGCCCAAGC CGGAGGGCTA CCGCAAGGCC CTGCGCCTGA TGAAGATGGC CGAGCGCTTC GGCCTGCCGG TGCTGACCCT GATCGACACC GCTGGCGCCT GGCCGGGCAT CGACGCCGAA TCGCGCGGCC AGTCCGAAGC GATCGCGCGC AACCTGATCG AGATGGCCGA ACTGAAGGTG CCGATCATCT GCACCGTGAT CGGCGAAGGC GGCTCCGGCG GCGCGCTGGC GCTGGGCGTG GGCGACCGCA CCGTGATGCT GGAATACGCG GTGTACTCGA CCATCACCCC GGAAGGCTGC GCCTCGATCC TGTGGAAGGA CGCCGGCAAG GCCAAGGACG CGGCCGAACA GCTGGGCCTG ACCGCCCCGC GCCTGAAGAG CCTGGGCCTG GTCGACAAGG TCGTGCGCGA GCCCACCGGC GGCGCCCACC GCAACCCGAC GCAGATGGCC AAGCGCCTGA AGGCGGTGCT GCTGAACGAG CTGGATGCGC TGGACAAGCT GTCCACCGAA CAGCTGCTGG AACAGCGCTA CGCGCGCCTG CGCAGCTACG GCACGTACGA AGCAGCC

Details for StmaA.18892.a.B1.GE43551

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN PNYLDFEQPI ADLEAKIQEL RNASAGPAVN VEAEVHALQD KLRMRTAQIF RNLTSWQVLQ LARHPSRPYT ADYLRVMFDE FQELAGDRAF ADDKAIMGGL ARINGRAVMV IGHQKGRDTK EKIKRNFGMP KPEGYRKALR LMKMAERFGL PVLTLIDTAG AWPGIDAESR GQSEAIARNL IEMAELKVPI ICTVIGEGGS GGALALGVGD RTVMLEYAVY STITPEGCAS ILWKDAGKAK DAAEQLGLTA PRLKSLGLVD KVVREPTGGA HRNPTQMAKR LKAVLLNELD ALDKLSTEQL LEQRYARLRS YGTYEAA

Validated NT Sequence

tactcactta ggctgcttcg tacgtgccgt agctgcgcag gcgcgcgtag cgctgttcca gcagctgttc ggtggacagc ttgtccagcg catccagctc gttcagcagc accgccttca ggcgcttggc catctgcgtc gggttgcggt gggcgccgcc ggtgggctcg cgcacgacct tgtcgaccag gcccaggctc ttcaggcgcg gggcggtcag gcccagctgt tcggccgcgt ccttggcctt gccggcgtcc ttccacagga tcgaggcgca gccttccggg gtgatggtcg agtacaccgc gtattccagc atcacggtgc ggtcgcccac gcccagcgcc agcgcgccgc cggagccgcc ttcgccgatc acggtgcaga tgatcggcac cttcagttcg gccatctcga tcaggttgcg cgcgatcgct tcggactggc cgcgcgattc ggcgtcgatg cccggccagg cgccagcggt gtcgatcagg gtcagcaccg gcaggccgaa gcgctcggcc atcttcatca ggcgcagggc cttgcggtag ccctccggct tgggcatgcc gaagttgcgc ttgatcttct ccttggtgtc acggcccttc tggtggccga tcaccatcac tgcacggccg ttgatgcggg ccaggccacc catgatcgcc ttgtcgtcgg cgaaggcacg gtcgccggcc agctcctgga actcatcgaa catgacgcgg aggtagtcgg cggtgtacgg gcgcgacgga tgacgggcca gctgcagcac ctgccacgag gtcaggttgc ggaagatctg cgcggtgcgc atgcgcagct tgtcctgcag cgcgtgcact tccgcctcga cattgaccgc cggcccggca ctggcgttgc gcagttcctg gatcttggct tccaggtcgg cgatgggttg ctcgaagtcg aggtagttcg gattcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc cgaactacct cgacttcgag caacccatcg ccgacctgga agccaagatc caggaactgc gcaacgccag tgccgggccg gcggtcaatg tcgaggcgga agtgcacgcg ctgcaggaca agctgcgcat gcgcaccgcg cagatcttcc gcaacctgac ctcgtggcag gtgctgcagc tggcccgtca tccgtcgcgc ccgtacaccg ccgactacct ccgcgtcatg ttcgatgagt tccaggagct ggccggcgac cgtgccttcg ccgacgacaa ggcgatcatg ggtggcctgg cccgcatcaa cggccgtgca gtgatggtga tcggccacca gaagggccgt gacaccaagg agaagatcaa gcgcaacttc ggcatgccca agccggaggg ctaccgcaag gccctgcgcc tgatgaagat ggccgagcgc ttcggcctgc cggtgctgac cctgatcgac accgctggcg cctggccggg catcgacgcc gaatcgcgcg gccagtccga agcgatcgcg cgcaacctga tcgagatggc cgaactgaag gtgccgatca tctgcaccgt gatcggcgaa ggcggctccg gcggcgcgct ggcgctgggc gtgggcgacc gcaccgtgat gctggaatac gcggtgtact cgaccatcac cccggaaggc tgcgcctcga tcctgtggaa ggacgccggc aaggccaagg acgcggccga acagctgggc ctgaccgccc cgcgcctgaa gagcctgggc ctggtcgaca aggtcgtgcg cgagcccacc ggcggcgccc accgcaaccc gacgcagatg gccaagcgcc tgaaggcggt gctgctgaac gagctggatg cgctggacaa gctgtccacc gaacagctgc tggaacagcg ctacgcgcgc ctgcgcagct acggcacgta cgaagcagcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat