StmaA.18890.a

Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) (pheT)

CENTER ID: StmaA.18890.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18890.a.B1.GE43550 Full length(StmaA.18890.a) 1 793
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3161

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFSENWLRS HVPTSASRDE LSAVLTAIGL EVEEVTALGE GLDHVVVARI VEAVRHPEAD RLQVCRVDAG QGELLQIVCG APNARPGLVA PLAMVGAQIG ELKIKPAKLR GVESNGMLCS AKELGLDSDA SGLLELPDDA PIGQTLVEYL GLPDASIEIK LTPNRADCFS LRGIAYDVAA ATRSEVLDFA ADAIAAVGSR ELSIQLDAGA EAPRYLGRVI EGVNAAARTP LWMAERLRRS GVRPVSLLVD ITQYVMLELG QPMHAYDLGT LQGSIAVRRS RAGETLKLLD GRDAALDDSF LVVTDADRAV GLAGLMGGFD TRVTDATTAV FLEAAHFAPA AIMGRGRKLG LHTDAGHRFE RGVDPALPRT AIEYATRLVL DLAGGTPAPV TEAVREADLP QPATIVLRRA RITRVLGISI DDAEVERILR ALGMDVVATA EGWQVTAPSR RFDIAIEEDL IEELARIQGY EQIPTTLPGG ASRVAMPSET QLDALSVRRQ LIARDQQETV NFAFVDDALL TQWQLRDGLV PLANPLSAEL AVMRPSLLPG LVATLGRNAA RQLGRVRLFE IGRVFAQQGG DGQPAPLETP RVAAAVCGEA QAVQWGLPTR KVDFHDLKGD LESLAAASGA QLEFRPSARA YGHPARSAEV FRDGVAIGWI GQIHPRLARA MEIEADVYAF ELDLEPLTAR RLPRAGELSR FPAVRRDLAF LVPEQVAWAD LAATVRQAAG PLLRDLNLFD RYVGQGVEPG FKSLAMGLIL QDKSRTLTDR DVDAVVAEAV TAIEREHHAR IRG
NT Sequence
ATGAAATTCT CCGAAAACTG GCTGCGCAGC CACGTCCCGA CCTCCGCCTC GCGCGATGAG CTGAGCGCGG TGCTGACCGC CATCGGCCTG GAAGTGGAAG AAGTAACCGC GCTTGGCGAA GGCCTCGATC ACGTGGTCGT GGCACGCATC GTTGAAGCCG TGCGCCATCC GGAAGCCGAC CGCCTGCAGG TGTGCAGGGT CGATGCCGGG CAGGGCGAAC TGCTGCAGAT CGTGTGCGGC GCACCGAACG CACGCCCGGG CCTGGTCGCG CCGCTGGCGA TGGTTGGTGC GCAGATCGGC GAGCTGAAGA TCAAGCCGGC CAAGCTGCGT GGCGTCGAGT CCAACGGCAT GCTCTGCTCG GCCAAGGAGC TGGGCCTGGA CAGCGATGCG TCCGGTCTGC TGGAACTGCC GGACGATGCC CCGATCGGCC AGACCCTGGT CGAGTACCTG GGCCTGCCGG ACGCCAGCAT CGAGATCAAG CTGACCCCGA ACCGCGCCGA CTGCTTCAGC CTGCGCGGCA TCGCCTACGA CGTCGCCGCC GCCACCCGCA GTGAAGTGCT GGATTTCGCC GCCGATGCCA TTGCGGCAGT CGGCAGCCGT GAGTTGAGCA TCCAGCTCGA CGCCGGCGCC GAAGCGCCGC GTTACCTGGG CCGTGTCATC GAAGGCGTCA ATGCTGCCGC CAGGACCCCG CTGTGGATGG CCGAGCGCCT GCGCCGCAGC GGCGTGCGTC CGGTCTCGCT GCTGGTCGAC ATCACCCAGT ACGTGATGCT GGAGCTGGGC CAGCCGATGC ACGCGTACGA CCTGGGCACC CTGCAGGGCA GCATCGCCGT GCGCCGTTCG CGCGCGGGCG AGACGCTGAA GCTGCTGGAC GGCCGCGATG CTGCGCTGGA CGACAGCTTC CTGGTGGTCA CCGATGCTGA CCGTGCGGTC GGCCTGGCCG GCCTGATGGG CGGCTTCGAC ACCCGCGTCA CCGACGCGAC CACCGCCGTG TTCCTGGAAG CGGCGCACTT CGCGCCGGCC GCGATCATGG GCCGTGGCCG CAAGCTGGGC CTGCACACCG ATGCCGGCCA TCGCTTCGAG CGCGGCGTCG ATCCGGCGCT GCCGCGCACC GCGATCGAGT ACGCCACCCG CCTGGTGCTG GACCTGGCCG GCGGTACCCC GGCACCGGTC ACCGAGGCCG TGCGTGAAGC CGATCTGCCG CAGCCGGCGA CCATCGTGCT GCGCCGCGCC CGCATCACCC GCGTGCTCGG CATCAGCATC GACGATGCCG AAGTCGAGCG CATCCTGCGC GCGCTGGGCA TGGACGTGGT TGCCACCGCC GAGGGCTGGC AGGTCACGGC GCCGAGCCGC CGCTTCGACA TCGCCATCGA AGAAGACCTG ATCGAAGAGC TGGCCCGCAT CCAAGGTTAC GAGCAGATCC CGACCACGCT GCCGGGCGGT GCCTCGCGCG TGGCGATGCC GAGCGAGACC CAGCTGGACG CGCTGAGCGT GCGCCGCCAG CTGATCGCCC GTGACCAGCA GGAAACCGTC AACTTCGCCT TCGTCGACGA CGCGCTGCTG ACCCAGTGGC AACTGCGCGA CGGTCTGGTG CCGCTGGCCA ACCCGCTGTC GGCGGAACTG GCGGTGATGC GTCCGTCGCT GCTGCCGGGC CTGGTCGCCA CGCTTGGCCG CAATGCCGCG CGCCAGCTGG GCCGCGTGCG CCTGTTCGAG ATCGGCCGTG TGTTTGCCCA GCAGGGCGGA GACGGCCAAC CGGCGCCGCT GGAAACCCCG CGCGTGGCCG CTGCGGTCTG CGGTGAGGCG CAGGCGGTGC AGTGGGGCCT GCCGACCCGC AAGGTCGATT TCCATGACCT GAAGGGCGAC CTGGAATCGC TGGCGGCTGC CAGTGGCGCG CAGCTGGAGT TCCGTCCGTC GGCCCGCGCC TACGGCCACC CGGCGCGTTC GGCCGAGGTG TTCCGCGATG GCGTGGCCAT CGGCTGGATC GGCCAGATCC ATCCGCGCCT GGCGCGGGCG ATGGAGATCG AGGCCGACGT GTACGCCTTC GAGCTGGACC TGGAGCCGCT GACCGCCCGT CGCCTGCCGC GTGCCGGCGA GCTGTCGCGC TTCCCGGCGG TGCGCCGCGA CCTGGCGTTC CTGGTGCCTG AGCAGGTGGC CTGGGCCGAC CTGGCGGCGA CCGTCCGCCA GGCCGCCGGC CCGCTGCTGC GCGACCTGAA CCTGTTCGAC CGCTATGTTG GCCAGGGCGT CGAGCCGGGC TTCAAGAGTC TCGCTATGGG CTTGATTTTG CAGGACAAGT CGCGCACTCT GACGGACCGC GACGTGGATG CGGTGGTGGC CGAGGCGGTC ACTGCCATCG AGCGTGAACA CCACGCCCGG ATCCGCGGC
Details for StmaA.18890.a.B1.GE43550
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 86kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK FSENWLRSHV PTSASRDELS AVLTAIGLEV EEVTALGEGL DHVVVARIVE AVRHPEADRL QVCRVDAGQG ELLQIVCGAP NARPGLVAPL AMVGAQIGEL KIKPAKLRGV ESNGMLCSAK ELGLDSDASG LLELPDDAPI GQTLVEYLGL PDASIEIKLT PNRADCFSLR GIAYDVAAAT RSEVLDFAAD AIAAVGSREL SIQLDAGAEA PRYLGRVIEG VNAAARTPLW MAERLRRSGV RPVSLLVDIT QYVMLELGQP MHAYDLGTLQ GSIAVRRSRA GETLKLLDGR DAALDDSFLV VTDADRAVGL AGLMGGFDTR VTDATTAVFL EAAHFAPAAI MGRGRKLGLH TDAGHRFERG VDPALPRTAI EYATRLVLDL AGGTPAPVTE AVREADLPQP ATIVLRRARI TRVLGISIDD AEVERILRAL GMDVVATAEG WQVTAPSRRF DIAIEEDLIE ELARIQGYEQ IPTTLPGGAS RVAMPSETQL DALSVRRQLI ARDQQETVNF AFVDDALLTQ WQLRDGLVPL ANPLSAELAV MRPSLLPGLV ATLGRNAARQ LGRVRLFEIG RVFAQQGGDG QPAPLETPRV AAAVCGEAQA VQWGLPTRKV DFHDLKGDLE SLAAASGAQL EFRPSARAYG HPARSAEVFR DGVAIGWIGQ IHPRLARAME IEADVYAFEL DLEPLTARRL PRAGELSRFP AVRRDLAFLV PEQVAWADLA ATVRQAAGPL LRDLNLFDRY VGQGVEPGFK SLAMGLILQD KSRTLTDRDV DAVVAEAVTA IEREHHARIR G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat tctccgaaaa ctggctgcgc agccacgtcc cgacctccgc ctcgcgcgat gagctgagcg cggtgctgac cgccatcggc ctggaagtgg aagaagtaac cgcgcttggc gaaggcctcg atcacgtggt cgtggcacgc atcgttgaag ccgtgcgcca tccggaagcc gaccgcctgc aggtgtgcag ggtcgatgcc gggcagggcg aactgctgca gatcgtgtgc ggcgcaccga acgcacgccc gggcctggtc gcgccgctgg cgatggttgg tgcgcagatc ggcgagctga agatcaagcc ggccaagctg cgtggcgtcg agtccaacgg catgctctgc tcggccaagg agctgggcct ggacagcgat gcgtccggtc tgctggaact gccggacgat gccccgatcg gccagaccct ggtcgagtac ctgggcctgc cggacgccag catcgagatc aagctgaccc cgaaccgcgc cgactgcttc agcctgcgcg gcatcgccta cgacgtcgcc gccgccaccc gcagtgaagt gctggatttc gccgccgatg ccattgcggc agtcggcagc cgtgagttga gcatccagct cgacgccggc gccgaagcgc cgcgttacct gggccgtgtc atcgaaggcg tcaatgctgc cgccaggacc ccgctgtgga tggccgagcg cctgcgccgc agcggcgtgc gtccggtctc gctgctggtc gacatcaccc agtacgtgat gctggagctg ggccagccga tgcacgcgta cgacctgggc accctgcagg gcagcatcgc cgtgcgccgt tcgcgcgcgg gcgagacgct gaagctgctg gacggccgcg atgctgcgct ggacgacagc ttcctggtgg tcaccgatgc tgaccgtgcg gtcggcctgg ccggcctgat gggcggcttc gacacccgcg tcaccgacgc gaccaccgcc gtgttcctgg aagcggcgca cttcgcgccg gccgcgatca tgggccgtgg ccgcaagctg ggcctgcaca ccgatgccgg ccatcgcttc gagcgcggcg tcgatccggc gctgccgcgc accgcgatcg agtacgccac ccgcctggtg ctggacctgg ccggcggtac cccggcaccg gtcaccgagg ccgtgcgtga agccgatctg ccgcagccgg cgaccatcgt gctgcgccgc gcccgcatca cccgcgtgct cggcatcagc atcgacgatg ccgaagtcga gcgcatcctg cgcgcgctgg gcatggacgt ggttgccacc gccgagggct ggcaggtcac ggcgccgagc cgccgcttcg acatcgccat cgaagaagac ctgatcgaag agctggcccg catccaaggt tacgagcaga tcccgaccac gctgccgggc ggtgcctcgc gcgtggcgat gccgagcgag acccagctgg acgcgctgag cgtgcgccgc cagctgatcg cccgtgacca gcaggaaacc gtcaacttcg ccttcgtcga cgacgcgctg ctgacccagt ggcaactgcg cgacggtctg gtgccgctgg ccaacccgct gtcggcggaa ctggcggtga tgcgtccgtc gctgctgccg ggcctggtcg ccacgcttgg ccgcaatgcc gcgcgccagc tgggccgcgt gcgcctgttc gagatcggcc gtgtgtttgc ccagcagggc ggagacggcc aaccggcgcc gctggaaacc ccgcgcgtgg ccgctgcggt ctgcggtgag gcgcaggcgg tgcagtgggg cctgccgacc cgcaaggtcg atttccatga cctgaagggc gacctggaat cgctggcggc tgccagtggc gcgcagctgg agttccgtcc gtcggcccgc gcctacggcc acccggcgcg ttcggccgag gtgttccgcg atggcgtggc catcggctgg atcggccaga tccatccgcg cctggcgcgg gcgatggaga tcgaggccga cgtgtacgcc ttcgagctgg acctggagcc gctgaccgcc cgtcgcctgc cgcgtgccgg cgagctgtcg cgcttcccgg cggtgcgccg cgacctggcg ttcctggtgc ctgagcaggt ggcctgggcc gacctggcgg cgaccgtccg ccaggccgcc ggcccgctgc tgcgcgacct gaacctgttc gaccgctatg ttggccaggg cgtcgagccg ggcttcaaga gtctcgctat gggcttgatt ttgcaggaca agtcgcgcac tctgacggac cgcgacgtgg atgcggtggt ggccgaggcg gtcactgcca tcgagcgtga acaccacgcc cggatccgcg gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat