StmaA.18889.a

Alanyl-tRNA synthetase (EC 6.1.1.7) (alaS)

CENTER ID: StmaA.18889.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18889.a.B1.GE43549 Full length(StmaA.18889.a) 1 882
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1666

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNASAKFTTS QIRSDFLEFF KGKGHTIVPS APLVPGNDPT LLFTNSGMVQ FKDVFLGAEK RSYVRAADVQ RCLRAGGKHN DLDQVGYTAR HHTFFEMLGN WSFGDYFKKD AIAWAWELLT QVWKLPAERL LVTVYQTDDE AYALWRDMVG VPEERIVRIG DNKGAPFASD NFWQMADTGP CGPCTEIFYD HGDHIAGGPP GSPDEDGDRF IEIWNLVFMQ FDRQPDGTLV PLPAPCVDTG MGLERLAAIL QHVHTNYEID LFQALIRKAS ELTGTADLEN KSLRVIADHI RACSFLIVDG VLPSNEGRGY VLRRIIRRAL RHGWMLGVRQ PFFSKLVPTL VEQMGEAYPE LPAAVDTVTR ALQAEEERFA ETLDAGMKIF EDVAGKASNG VIPGVDAFRL YDTYGFPLDL TQDIARERDL TVDIAGFDAA MEQQRETARA AGKFGGGVTL PAELVATLSP TLFLGYDRLQ ADGLTVLALL KDGRPVQSAD AGDAVIVITN QTPFYAESGG QVGDTGVLTG NGVRLAVDDT QKFAGQFHGH VGTLSEGGLK VGDVLSGQVD GERRGATILN HSATHLLHAA LREVLGSHVQ QKGSLVAPDR LRFDFSHFQP ISAEELAVIE RKVNQQVRAN NAAEVHNMGM QEALDFGAMA LFGEKYGEHV RVLKMGDYST ELCGGTHVNR TGDIGLFKIT SEGGVSAGVR RIEAVTGQGA LDYVDAEEAR LAEAAELLGG SAADVVEKIR ALGQRQKQLE RELEAVKAKV AAGATADLSG QAVEVAGVKV LAARLEGFDA KALRDAMDRL KQQLGDAVIV LAGAQDGKAA LVAGVNGSAM GKVKAGELLS HIASQIGGKG GGRPDLAQGG GEDGPALATA LAAVVEWVSP RL
NT Sequence
ATGAACGCAT CCGCCAAATT CACGACTTCC CAGATCCGCA GCGACTTCCT TGAATTCTTC AAGGGCAAAG GCCACACCAT CGTGCCGTCG GCGCCGCTGG TGCCGGGCAA TGATCCGACC CTGCTGTTCA CCAACTCCGG CATGGTGCAG TTCAAGGACG TGTTCCTTGG CGCGGAAAAG CGCAGCTACG TGCGCGCGGC CGACGTCCAG CGCTGCCTGC GCGCGGGCGG CAAGCACAAC GACCTCGACC AGGTCGGCTA CACCGCGCGC CACCACACTT TCTTCGAAAT GCTGGGCAAC TGGTCGTTCG GCGACTACTT CAAGAAGGAC GCGATCGCCT GGGCCTGGGA ACTGCTGACC CAGGTCTGGA AGCTGCCGGC CGAACGCCTG CTGGTCACCG TCTACCAGAC CGATGACGAA GCCTACGCGC TGTGGCGCGA CATGGTCGGC GTGCCGGAAG AGCGCATCGT GCGCATCGGC GACAACAAGG GCGCACCGTT TGCCTCGGAC AACTTCTGGC AGATGGCCGA CACCGGCCCG TGCGGCCCGT GCACCGAAAT CTTCTACGAC CACGGTGACC ATATCGCCGG TGGCCCCCCG GGTTCCCCGG ATGAAGACGG CGACCGCTTC ATCGAAATCT GGAACCTGGT GTTCATGCAG TTCGACCGCC AGCCCGACGG CACCCTGGTG CCACTGCCGG CGCCGTGCGT GGATACCGGC ATGGGCCTGG AGCGCCTGGC GGCGATCCTG CAGCACGTGC ACACCAACTA TGAGATCGAC CTGTTCCAGG CCCTGATCCG CAAGGCCTCC GAGCTGACCG GCACCGCCGA CCTGGAAAAC AAGTCGCTGC GCGTGATCGC CGACCACATC CGTGCCTGTT CGTTCCTGAT CGTCGATGGC GTGCTGCCGT CCAACGAAGG CCGCGGTTAC GTGCTGCGCC GTATCATCCG CCGCGCACTG CGCCACGGCT GGATGCTGGG CGTGCGCCAG CCGTTCTTCA GCAAGCTGGT GCCGACCCTG GTCGAGCAGA TGGGCGAAGC CTATCCGGAA CTGCCGGCTG CGGTGGACAC CGTCACCCGT GCGCTGCAGG CCGAAGAAGA GCGTTTCGCC GAAACCCTCG ATGCCGGCAT GAAGATCTTC GAGGACGTCG CGGGCAAGGC CAGCAATGGC GTGATCCCGG GCGTTGACGC CTTCCGCCTG TACGACACCT ATGGTTTCCC GCTCGACCTG ACCCAGGACA TCGCCCGCGA GCGCGACCTG ACCGTCGACA TTGCCGGCTT CGATGCCGCG ATGGAGCAGC AGCGCGAGAC CGCGCGCGCG GCCGGCAAGT TCGGTGGTGG CGTGACCCTG CCCGCTGAGC TGGTGGCGAC CCTGAGCCCG ACCCTGTTCC TGGGCTACGA CCGCCTGCAG GCTGACGGCT TGACCGTGCT GGCCCTGCTC AAGGACGGCC GTCCGGTGCA GTCGGCCGAT GCCGGTGACG CAGTCATCGT CATCACCAAC CAGACCCCGT TCTACGCCGA GTCCGGCGGC CAGGTCGGTG ACACCGGCGT ACTGACCGGC AACGGCGTGC GCCTGGCGGT GGACGACACC CAGAAGTTCG CCGGCCAGTT CCATGGCCAT GTCGGCACCC TGTCCGAAGG CGGCCTGAAG GTCGGCGACG TGCTCTCCGG CCAGGTCGAT GGCGAGCGCC GTGGCGCCAC CATCCTCAAC CACTCGGCCA CCCACCTGCT GCACGCCGCC CTGCGCGAAG TGCTGGGCAG CCACGTGCAG CAGAAGGGCT CGCTGGTCGC TCCGGACCGC CTGCGTTTCG ACTTCTCGCA CTTCCAGCCG ATCAGCGCGG AAGAGCTTGC CGTGATCGAG CGCAAGGTCA ACCAGCAGGT GCGCGCCAAC AACGCCGCCG AAGTACACAA CATGGGCATG CAGGAAGCGC TGGACTTCGG CGCGATGGCC CTGTTCGGCG AGAAGTACGG CGAGCACGTG CGCGTGTTGA AGATGGGTGA CTACTCCACC GAACTGTGCG GTGGTACCCA CGTCAACCGT ACCGGCGACA TCGGTCTGTT CAAGATCACC TCCGAGGGCG GCGTCTCCGC GGGCGTGCGC CGCATCGAGG CTGTTACCGG CCAGGGTGCG CTGGATTACG TGGACGCCGA AGAGGCCCGC CTGGCCGAGG CCGCTGAACT GCTCGGTGGC AGCGCTGCCG ACGTGGTCGA GAAGATCCGT GCCCTCGGCC AGCGCCAGAA GCAGCTGGAG CGCGAACTGG AGGCCGTGAA GGCCAAGGTC GCCGCCGGTG CCACGGCCGA TCTGTCCGGC CAGGCCGTCG AGGTTGCTGG CGTGAAGGTA CTGGCTGCCC GCCTGGAGGG CTTCGACGCC AAGGCCCTGC GTGACGCCAT GGACCGCCTC AAGCAGCAGC TGGGCGACGC GGTGATCGTG CTGGCCGGCG CCCAGGACGG CAAGGCCGCC CTGGTCGCGG GTGTGAACGG CAGTGCAATG GGCAAGGTCA AGGCCGGGGA ACTGTTGTCC CATATCGCCA GTCAGATCGG GGGCAAGGGC GGCGGACGCC CGGACCTCGC CCAGGGTGGT GGCGAGGACG GGCCCGCCCT TGCCACTGCG CTGGCTGCAG TCGTCGAATG GGTCAGCCCC CGCCTG
Details for StmaA.18889.a.B1.GE43549
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 96kDa
OBSERVED MW: 96kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
MAHHHHHHMN ASAKFTTSQI RSDFLEFFKG KGHTIVPSAP LVPGNDPTLL FTNSGMVQFK DVFLGAEKRS YVRAADVQRC LRAGGKHNDL DQVGYTARHH TFFEMLGNWS FGDYFKKDAI AWAWELLTQV WKLPAERLLV TVYQTDDEAY ALWRDMVGVP EERIVRIGDN KGAPFASDNF WQMADTGPCG PCTEIFYDHG DHIAGGPPGS PDEDGDRFIE IWNLVFMQFD RQPDGTLVPL PAPCVDTGMG LERLAAILQH VHTNYEIDLF QXLIRKAXXL TGTAXLXTSR CA
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN ASAKFTTSQI RSDFLEFFKG KGHTIVPSAP LVPGNDPTLL FTNSGMVQFK DVFLGAEKRS YVRAADVQRC LRAGGKHNDL DQVGYTARHH TFFEMLGNWS FGDYFKKDAI AWAWELLTQV WKLPAERLLV TVYQTDDEAY ALWRDMVGVP EERIVRIGDN KGAPFASDNF WQMADTGPCG PCTEIFYDHG DHIAGGPPGS PDEDGDRFIE IWNLVFMQFD RQPDGTLVPL PAPCVDTGMG LERLAAILQH VHTNYEIDLF QALIRKASEL TGTADLENKS LRVIADHIRA CSFLIVDGVL PSNEGRGYVL RRIIRRALRH GWMLGVRQPF FSKLVPTLVE QMGEAYPELP AAVDTVTRAL QAEEERFAET LDAGMKIFED VAGKASNGVI PGVDAFRLYD TYGFPLDLTQ DIARERDLTV DIAGFDAAME QQRETARAAG KFGGGVTLPA ELVATLSPTL FLGYDRLQAD GLTVLALLKD GRPVQSADAG DAVIVITNQT PFYAESGGQV GDTGVLTGNG VRLAVDDTQK FAGQFHGHVG TLSEGGLKVG DVLSGQVDGE RRGATILNHS ATHLLHAALR EVLGSHVQQK GSLVAPDRLR FDFSHFQPIS AEELAVIERK VNQQVRANNA AEVHNMGMQE ALDFGAMALF GEKYGEHVRV LKMGDYSTEL CGGTHVNRTG DIGLFKITSE GGVSAGVRRI EAVTGQGALD YVDAEEARLA EAAELLGGSA ADVVEKIRAL GQRQKQLERE LEAVKAKVAA GATADLSGQA VEVAGVKVLA ARLEGFDAKA LRDAMDRLKQ QLGDAVIVLA GAQDGKAALV AGVNGSAMGK VKAGELLSHI ASQIGGKGGG RPDLAQGGGE DGPALATALA AVVEWVSPRL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg catccgccaa attcacgact tcccagatcc gcagcgactt ccttgaattc ttcaagggca aaggccacac catcgtgccg tcggcgccgc tggtgccggg caatgatccg accctgctgt tcaccaactc cggcatggtg cagttcaagg acgtgttcct tggcgcggaa aagcgcagct acgtgcgcgc ggccgacgtc cagcgctgcc tgcgcgcggg cggcaagcac aacgacctcg accaggtcgg ctacaccgcg cgccaccaca ctttcttcga aatgctgggc aactggtcgt tcggcgacta cttcaagaag gacgcgatcg cctgggcctg ggaactgctg acccaggtct ggaagctgcc ggccgaacgc ctgctggtca ccgtctacca gaccgatgac gaagcctacg cgctgtggcg cgacatggtc ggcgtgccgg aagagcgcat cgtgcgcatc ggcgacaaca agggcgcacc gtttgcctcg gacaacttct ggcagatggc cgacaccggc ccgtgcggcc cgtgcaccga aatcttctac gaccacggtg accatatcgc cggtggcccc ccgggttccc cggatgaaga cggcgaccgc ttcatcgaaa tctggaacct ggtgttcatg cagttcgacc gccagcccga cggcaccctg gtgccactgc cggcgccgtg cgtggatacc ggcatgggcc tggagcgcct ggcggcgatc ctgcagcacg tgcacaccaa ctatgagatc gacctgttcc aggccctgat ccgcaaggcc tccgagctga ccggcaccgc cgacctggaa aacaagtcgc tgcgcgtgat cgccgaccac atccgtgcct gttcgttcct gatcgtcgat ggcgtgctgc cgtccaacga aggccgcggt tacgtgctgc gccgtatcat ccgccgcgca ctgcgccacg gctggatgct gggcgtgcgc cagccgttct tcagcaagct ggtgccgacc ctggtcgagc agatgggcga agcctatccg gaactgccgg ctgcggtgga caccgtcacc cgtgcgctgc aggccgaaga agagcgtttc gccgaaaccc tcgatgccgg catgaagatc ttcgaggacg tcgcgggcaa ggccagcaat ggcgtgatcc cgggcgttga cgccttccgc ctgtacgaca cctatggttt cccgctcgac ctgacccagg acatcgcccg cgagcgcgac ctgaccgtcg acattgccgg cttcgatgcc gcgatggagc agcagcgcga gaccgcgcgc gcggccggca agttcggtgg tggcgtgacc ctgcccgctg agctggtggc gaccctgagc ccgaccctgt tcctgggcta cgaccgcctg caggctgacg gcttgaccgt gctggccctg ctcaaggacg gccgtccggt gcagtcggcc gatgccggtg acgcagtcat cgtcatcacc aaccagaccc cgttctacgc cgagtccggc ggccaggtcg gtgacaccgg cgtactgacc ggcaacggcg tgcgcctggc ggtggacgac acccagaagt tcgccggcca gttccatggc catgtcggca ccctgtccga aggcggcctg aaggtcggcg acgtgctctc cggccaggtc gatggcgagc gccgtggcgc caccatcctc aaccactcgg ccacccacct gctgcacgcc gccctgcgcg aagtgctggg cagccacgtg cagcagaagg gctcgctggt cgctccggac cgcctgcgtt tcgacttctc gcacttccag ccgatcagcg cggaagagct tgccgtgatc gagcgcaagg tcaaccagca ggtgcgcgcc aacaacgccg ccgaagtaca caacatgggc atgcaggaag cgctggactt cggcgcgatg gccctgttcg gcgagaagta cggcgagcac gtgcgcgtgt tgaagatggg tgactactcc accgaactgt gcggtggtac ccacgtcaac cgtaccggcg acatcggtct gttcaagatc acctccgagg gcggcgtctc cgcgggcgtg cgccgcatcg aggctgttac cggccagggt gcgctggatt acgtggacgc cgaagaggcc cgcctggccg aggccgctga actgctcggt ggcagcgctg ccgacgtggt cgagaagatc cgtgccctcg gccagcgcca gaagcagctg gagcgcgaac tggaggccgt gaaggccaag gtcgccgccg gtgccacggc cgatctgtcc ggccaggccg tcgaggttgc tggcgtgaag gtactggctg cccgcctgga gggcttcgac gccaaggccc tgcgtgacgc catggaccgc ctcaagcagc agctgggcga cgcggtgatc gtgctggccg gcgcccagga cggcaaggcc gccctggtcg cgggtgtgaa cggcagtgca atgggcaagg tcaaggccgg ggaactgttg tcccatatcg ccagtcagat cgggggcaag ggcggcggac gcccggacct cgcccagggt ggtggcgagg acgggcccgc ccttgccact gcgctggctg cagtcgtcga atgggtcagc ccccgcctgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat