StmaA.18887.a

D-arabinose-5-phosphate isomerase (EC 5.3.1.13) (kdsD)

CENTER ID: StmaA.18887.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18887.a.B1.GE43548 Full length(StmaA.18887.a) 1 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1080

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAESLLPPRS VDPAGLVASG RRVFEIERQA LDAVADRLGE AFQQACQAIL ASRGRVVATG MGKSGHIARK IAATLASTGT PAFYVHPGEA GHGDLGMITE DDVVLALSYS GESDEVLMLL PVLKRQGNLL ISMTGRPQSS LAAAADIHLD VSVPAEACPL ALAPTSSTTA SLAMGDALAV ALLDARGFTA DDFARSHPAG SLGRRLLLHI TDVMHTGDDL PSVGADASLS EALVEMSRKR LGMTAVVDAD GVLIGLFTDG DLRRALDSAL DVRTAKIADV MTRNPRTIGA DQLAVEAARL METHKITGLI VVDGQGRAVG ALNIHDLLRA RVV
NT Sequence
ATGGCCGAAT CCCTGTTGCC CCCCCGTTCC GTCGACCCTG CCGGCCTGGT TGCCAGTGGC CGCCGCGTGT TCGAGATCGA GCGGCAGGCG CTGGATGCCG TGGCCGACCG CCTTGGCGAG GCCTTCCAGC AGGCCTGCCA GGCGATTCTG GCCAGCCGCG GGCGGGTGGT CGCCACCGGC ATGGGCAAGT CCGGGCATAT CGCGCGCAAG ATCGCCGCCA CCCTGGCCTC TACCGGTACG CCGGCGTTCT ACGTGCACCC CGGTGAAGCC GGCCACGGCG ACCTGGGCAT GATCACCGAG GACGACGTCG TGCTGGCCCT GTCCTACTCG GGCGAATCGG ACGAGGTGCT GATGCTGCTG CCGGTGCTCA AGCGCCAGGG CAACCTGCTG ATCTCCATGA CCGGCCGCCC GCAGTCCAGC CTGGCCGCCG CCGCCGACAT CCATCTGGAT GTGAGCGTGC CGGCCGAGGC CTGCCCGCTG GCGCTGGCAC CCACTTCCAG CACCACCGCC TCGCTGGCGA TGGGCGATGC ACTGGCCGTG GCCCTGCTCG ATGCACGTGG CTTCACCGCC GATGACTTCG CCCGCTCGCA CCCGGCCGGC AGCCTCGGCC GTCGCCTGCT GCTGCACATC ACCGACGTCA TGCACACCGG CGACGACCTG CCCAGCGTGG GCGCCGACGC CAGCCTCAGC GAGGCGCTGG TGGAAATGAG CCGCAAGCGG CTGGGCATGA CCGCCGTGGT TGACGCCGAC GGCGTGCTGA TCGGCCTGTT CACCGATGGC GACCTGCGCC GTGCGCTGGA CAGCGCGCTG GATGTGCGCA CGGCGAAGAT CGCCGACGTG ATGACCCGCA ACCCGCGCAC CATCGGCGCC GACCAGCTGG CGGTCGAGGC CGCCCGCCTG ATGGAGACCC ACAAGATCAC CGGCCTGATC GTGGTTGATG GCCAGGGCCG CGCGGTTGGC GCGCTGAACA TTCATGACCT GTTGCGGGCC CGGGTGGTT
Details for StmaA.18887.a.B1.GE43548
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ESLLPPRSVD PAGLVASGRR VFEIERQALD AVADRLGEAF QQACQAILAS RGRVVATGMG KSGHIARKIA ATLASTGTPA FYVHPGEAGH GDLGMITEDD VVLALSYSGE SDEVLMLLPV LKRQGNLLIS MTGRPQSSLA AAADIHLDVS VPAEACPLAL APTSSTTASL AMGDALAVAL LDARGFTADD FARSHPAGSL GRRLLLHITD VMHTGDDLPS VGADASLSEA LVEMSRKRLG MTAVVDADGV LIGLFTDGDL RRALDSALDV RTAKIADVMT RNPRTIGADQ LAVEAARLME THKITGLIVV DGQGRAVGAL NIHDLLRARV V
Validated NT Sequence
aaaccacccg ggcccgcaac aggtcatgaa tgttcagcgc gccaaccgcg cggccctggc catcaaccac gatcaggccg gtgatcttgt gggtctccat caggcgggcg gcctcgaccg ccagctggtc ggcgccgatg gtgcgcgggt tgcgggtcat cacgtcggcg atcttcgccg tgcgcacatc cagcgcgctg tccagcgcac ggcgcaggtc gccatcggtg aacaggccga tcagcacgcc gtcggcgtca accacggcgg tcatgcccag ccgcttgcgg ctcatttcca ccagcgcctc gctgaggctg gcgtcggcgc ccacgctggg caggtcgtcg ccggtgtgca tgacgtcggt gatgtgcagc agcaggcgac ggccgaggct gccggccggg tgcgagcggg cgaagtcatc ggcggtgaag ccacgtgcat cgagcagggc cacggccagt gcatcgccca tcgccagcga ggcggtggtg ctggaagtgg gtgccagcgc cagcgggcag gcctcggccg gcacgctcac atccagatgg atgtcggcgg cggcggccag gctggactgc gggcggccgg tcatggagat cagcaggttg ccctggcgct tgagcaccgg cagcagcatc agcacctcgt ccgattcgcc cgagtaggac agggccagca cgacgtcgtc ctcggtgatc atgcccaggt cgccgtggcc ggcttcaccg gggtgcacgt agaacgccgg cgtaccggta gaggccaggg tggcggcgat cttgcgcgcg atatgcccgg acttgcccat gccggtggcg accacccgcc cgcggctggc cagaatcgcc tggcaggcct gctggaaggc ctcgccaagg cggtcggcca cggcatccag cgcctgccgc tcgatctcga acacgcggcg gccactggca accaggccgg cagggtcgac ggaacggggg ggcaacaggg attcggccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMA ESLLPPRSVD PAGLVASGRR VFEIERQALD AVADRLGEAF QQACQAILAS RGRVVATGMG KSGHIARKIA ATLASTGTPA FYVHPGEAGH GDLGMITEDD VVLALSYSGE SDEVLMLLPV LKRQGNLLIS MTGRPQSSLA AAADIHLDVS VPAEACPLAL APTSSTTASL AMGDALAVAL LDARGFTADD FARSHPAGSL GRRLLLHITD VMHTGDDLPS VGADASLSEA LVEMSRKRLG MTAVVDADGV LIGLFTDGDL RRALDSALDV RTAKIADVMT RNPRTIGADQ LAVEAARLME THKITGLIVV DGQGRAVGAL NIHDLLRARV V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg aatccctgtt gcccccccgt tccgtcgacc ctgccggcct ggttgccagt ggccgccgcg tgttcgagat cgagcggcag gcgctggatg ccgtggccga ccgccttggc gaggccttcc agcaggcctg ccaggcgatt ctggccagcc gcgggcgggt ggtcgccacc ggcatgggca agtccgggca tatcgcgcgc aagatcgccg ccaccctggc ctctaccggt acgccggcgt tctacgtgca ccccggtgaa gccggccacg gcgacctggg catgatcacc gaggacgacg tcgtgctggc cctgtcctac tcgggcgaat cggacgaggt gctgatgctg ctgccggtgc tcaagcgcca gggcaacctg ctgatctcca tgaccggccg cccgcagtcc agcctggccg ccgccgccga catccatctg gatgtgagcg tgccggccga ggcctgcccg ctggcgctgg cacccacttc cagcaccacc gcctcgctgg cgatgggcga tgcactggcc gtggccctgc tcgatgcacg tggcttcacc gccgatgact tcgcccgctc gcacccggcc ggcagcctcg gccgtcgcct gctgctgcac atcaccgacg tcatgcacac cggcgacgac ctgcccagcg tgggcgccga cgccagcctc agcgaggcgc tggtggaaat gagccgcaag cggctgggca tgaccgccgt ggttgacgcc gacggcgtgc tgatcggcct gttcaccgat ggcgacctgc gccgtgcgct ggacagcgcg ctggatgtgc gcacggcgaa gatcgccgac gtgatgaccc gcaacccgcg caccatcggc gccgaccagc tggcggtcga ggccgcccgc ctgatggaga cccacaagat caccggcctg atcgtggttg atggccaggg ccgcgcggtt ggcgcgctga acattcatga cctgttgcgg gcccgggtgg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat