StmaA.18873.a

Diaminopimelate epimerase (EC 5.1.1.7) (dapF)

CENTER ID: StmaA.18873.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18873.a.B1.GE43546 Full length(StmaA.18873.a) 1 283
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3850

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKAGSKALR FSKMHGAGND FVVIDLRDGT APPTPELAAQ LADRHTGVGC DQILTIEAPR AEGSVASYRI WNADGSNSEQ CGNGARCIAA WLVREGSAQG DRFVIDSPLA SHEVEVLGDG QFAVTMGVPA FEPANVPLVG FAHAREEYLL PLQGESVRFA AVSMGNPHAV IEVGLIDAAP VERVGALLQQ HASFPRSVNV GFAQVMGPGH ARLRVNERGV GETLACGSGA CAAAVTLMQR GRLQRDARIS LPGGDLRIQW PGDGQPVVMA GPTAFVFEGE WMA
NT Sequence
ATGAGTAAGG CCGGTTCCAA GGCCCTGCGC TTCAGCAAGA TGCATGGCGC GGGCAATGAT TTCGTAGTGA TCGACCTGCG TGACGGCACC GCGCCGCCCA CGCCTGAACT GGCCGCGCAG CTGGCCGACC GCCACACCGG CGTCGGCTGC GATCAGATCC TGACCATCGA AGCGCCGCGT GCGGAAGGCT CGGTGGCGTC GTACCGCATC TGGAATGCCG ACGGCTCCAA TTCCGAACAG TGCGGCAATG GCGCGCGCTG CATCGCCGCC TGGCTGGTGC GCGAAGGCAG TGCGCAGGGC GACCGCTTCG TCATCGACAG CCCGCTGGCC AGCCATGAGG TGGAAGTGCT GGGCGATGGC CAGTTCGCGG TGACCATGGG CGTGCCGGCG TTCGAGCCGG CCAACGTGCC GCTGGTGGGT TTCGCCCACG CGCGCGAGGA ATACCTGCTG CCGCTGCAGG GCGAAAGCGT GCGTTTCGCT GCGGTGTCGA TGGGCAACCC GCATGCGGTG ATCGAAGTGG GCCTGATCGA TGCTGCACCG GTCGAGCGCG TCGGTGCGCT GCTGCAGCAG CATGCCTCGT TCCCGCGCTC GGTGAACGTG GGCTTCGCCC AGGTGATGGG CCCGGGGCAT GCACGCCTGC GTGTGAATGA ACGCGGCGTC GGCGAAACCC TGGCCTGCGG CAGTGGCGCC TGCGCCGCTG CGGTGACCCT GATGCAGCGC GGCCGCCTGC AGCGTGATGC ACGCATCAGC CTGCCCGGCG GTGACCTGCG CATCCAGTGG CCGGGCGATG GCCAGCCTGT GGTGATGGCC GGCCCGACCG CATTCGTCTT CGAAGGGGAG TGGATGGCA
Details for StmaA.18873.a.B1.GE43546
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS KAGSKALRFS KMHGAGNDFV VIDLRDGTAP PTXELAAQLA DRHTGVGCDQ ILTIEAPRAE GSVASYRIWN ADGSNSEQCG NGARCIAAWL VREGSAQGDR FVIDSPLASH EVEVLGDGQF AVTMGVPAFE PANVPLVGFA HAREEYLLPL QGESVRFAAV SMGNPHAVIE VGLIDAAPVE RVGALLQQHA SFPRSVNVGF AQVMGPGHAR LRVNERGVGE TLACGSGACA AAVTLMQRGR LQRDARISLP GGDLRIQWPG DGQPVVMAGP TAFVFEGEWM A
Validated NT Sequence
atggctcacc accaccacca ccatatgagt aaggccggtt ccaaggccct gcgcttcagc aagatgcatg gcgcgggcaa tgatttcgta gtgatcgacc tgcgtgacgg caccgcgccg cccacgcntg aactggccgc gcagctggcc gaccgccaca ccggcgtcgg ctgcgatcag atcctgacca tcgaagcgcc gcgtgcggaa ggctcggtgg cgtcgtaccg catctggaat gccgacggct ccaattccga acagtgcggc aatggcgcgc gctgcatcgc cgcctggctg gtgcgcgaag gcagtgcgca gggcgaccgc ttcgtcatcg acagcccgct ggccagccat gaggtggaag tgctgggcga tggccagttc gcggtgacca tgggcgtgcc ggcgttcgag ccggccaacg tgccgctggt gggtttcgcc cacgcgcgcg aggaatacct gctgccgctg cagggcgaaa gcgtgcgttt cgctgcggtg tcgatgggca acccgcatgc ggtgatcgaa gtgggcctga tcgatgctgc accggtcgag cgcgtcggtg cgctgctgca gcagcatgcc tcgttcccgc gctcggtgaa cgtgggcttc gcccaggtga tgggcccggg gcatgcacgc ctgcgtgtga atgaacgcgg cgtcggcgaa accctggcct gcggcagtgg cgcctgcgcc gctgcggtga ccctgatgca gcgcggccgc ctgcagcgtg atgcacgcat cagcctgccc ggcggtgacc tgcgcatcca gtggccgggc gatggccagc ctgtggtgat ggccggcccg accgcattcg tcttcgaagg ggagtggatg gcataa
Expected Protein Sequence
MAHHHHHHMS KAGSKALRFS KMHGAGNDFV VIDLRDGTAP PTPELAAQLA DRHTGVGCDQ ILTIEAPRAE GSVASYRIWN ADGSNSEQCG NGARCIAAWL VREGSAQGDR FVIDSPLASH EVEVLGDGQF AVTMGVPAFE PANVPLVGFA HAREEYLLPL QGESVRFAAV SMGNPHAVIE VGLIDAAPVE RVGALLQQHA SFPRSVNVGF AQVMGPGHAR LRVNERGVGE TLACGSGACA AAVTLMQRGR LQRDARISLP GGDLRIQWPG DGQPVVMAGP TAFVFEGEWM A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta aggccggttc caaggccctg cgcttcagca agatgcatgg cgcgggcaat gatttcgtag tgatcgacct gcgtgacggc accgcgccgc ccacgcctga actggccgcg cagctggccg accgccacac cggcgtcggc tgcgatcaga tcctgaccat cgaagcgccg cgtgcggaag gctcggtggc gtcgtaccgc atctggaatg ccgacggctc caattccgaa cagtgcggca atggcgcgcg ctgcatcgcc gcctggctgg tgcgcgaagg cagtgcgcag ggcgaccgct tcgtcatcga cagcccgctg gccagccatg aggtggaagt gctgggcgat ggccagttcg cggtgaccat gggcgtgccg gcgttcgagc cggccaacgt gccgctggtg ggtttcgccc acgcgcgcga ggaatacctg ctgccgctgc agggcgaaag cgtgcgtttc gctgcggtgt cgatgggcaa cccgcatgcg gtgatcgaag tgggcctgat cgatgctgca ccggtcgagc gcgtcggtgc gctgctgcag cagcatgcct cgttcccgcg ctcggtgaac gtgggcttcg cccaggtgat gggcccgggg catgcacgcc tgcgtgtgaa tgaacgcggc gtcggcgaaa ccctggcctg cggcagtggc gcctgcgccg ctgcggtgac cctgatgcag cgcggccgcc tgcagcgtga tgcacgcatc agcctgcccg gcggtgacct gcgcatccag tggccgggcg atggccagcc tgtggtgatg gccggcccga ccgcattcgt cttcgaaggg gagtggatgg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat