SSGCID: Target Page

StmaA.18854.a

Aconitate hydratase 2 (EC 4.2.1.3) (acnB)

CENTER ID:	StmaA.18854.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.18854.a.B1.GE43542	Full length(StmaA.18854.a)		1	863

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.18854.a.B1.PS38637	Full length(StmaA.18854.a)		1	863

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.2140

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLEAYRHHVA ERAALGIPPL PLSAQQTADV IELLKNPPQG EAEFLLDLLT HRVPAGVDDA AKVKASYLAA IALGSEQNPL ISRERATELL GTMLGGYNVA PLVQLLDDTS AGTIAANGLK KTLLVFDAFH DVQEKAKAGN ANAQAVLQSW ADAEWFTSNP EVPQSLTVTV FKVPGETNTD DLSPAPDATT RPDIPMHALA MLKNKRDDAP FTPEEDGKRG PIQQILDLKD KGHLVAYVGD VVGTGSSRKS ATNSVLWWTG DDIPFIPNKR AGGVCLGSKI APIFYNTMED AGALPIELDV SKMEHGDVVE LRPYEGKALK NGEVIAEFQV KSEVLFDEVR AGGRIPLIIG RGLTGKAREA LGLAPTNLFR LPVQPADTGK GFSLAQKMVG RACGLPEGQG MRPGTYCEPK MTSVGSQDTT GPMTRDELKD LACLGFSADL VMQSFCHTAA YPKPVDVKTH HTLPEFISTR GGVSLRPGDG VIHSWLNRML LPDTVGTGGD SHTRFPVGIS FPAGSGLVAF AAATGVMPLD MPESVLVRFK GKMQPGVTLR DLVNAIPLYA IKSGLLTVAK AGKKNIFSGR ILEIEGLPEL KVEQAFELSD ASAERSAAGC SVRLNKEPII EYLTSNITLL KWMIAEGYQD PRSLQRRIEK MEAWLANPQL LEPDADAEYA AVIEIDLADI HEPIVACPND PDDVKTLSEV AGAKIDEVFI GSCMTNIGHF RAAAKLLEGK RDLPTRLWVA PPTKMDASEL TKEGVYGTFG ATGARMEMPG CSLCMGNQAQ IREGSTAMST STRNFPNRLG RNTNVYLGSA ELAAICSRLG RIPTKEEYMA DIGVINANGA EIYRYMNFDQ IEEYQDVANT VAA

NT Sequence

ATGTTGGAAG CCTACCGCCA CCACGTCGCC GAGCGCGCTG CGCTTGGCAT CCCGCCGCTG CCGCTGAGCG CGCAGCAGAC GGCCGATGTC ATTGAACTGC TGAAGAACCC GCCGCAGGGC GAGGCCGAGT TCCTGCTCGA CCTGCTGACC CACCGCGTGC CGGCCGGCGT CGATGACGCA GCCAAGGTCA AGGCCTCGTA CCTGGCCGCG ATCGCGCTGG GCAGCGAGCA GAACCCGCTG ATCAGCCGCG AGCGCGCCAC CGAACTTCTG GGCACCATGC TCGGCGGTTA CAACGTTGCG CCGTTGGTGC AGCTGCTTGA CGACACCAGC GCCGGCACCA TTGCTGCCAA TGGCCTGAAG AAGACCCTGC TGGTGTTCGA CGCGTTCCAC GACGTGCAGG AAAAGGCCAA GGCCGGCAAC GCCAACGCCC AGGCCGTGCT GCAGAGCTGG GCCGATGCCG AGTGGTTCAC CAGCAACCCG GAAGTGCCGC AGAGCCTGAC CGTCACCGTG TTCAAGGTGC CTGGCGAGAC CAACACCGAC GACCTGTCGC CGGCACCGGA TGCGACCACC CGCCCGGACA TCCCGATGCA CGCCCTGGCG ATGCTGAAGA ACAAGCGCGA CGACGCGCCG TTCACCCCGG AAGAAGACGG CAAGCGCGGC CCGATCCAGC AGATCCTCGA CCTGAAGGAC AAGGGCCACC TGGTTGCCTA TGTCGGCGAC GTGGTCGGTA CCGGCTCCTC GCGCAAGTCG GCCACCAACA GCGTGCTGTG GTGGACCGGT GATGACATTC CGTTCATCCC GAACAAGCGC GCCGGTGGCG TCTGCCTGGG TTCGAAGATC GCCCCGATCT TCTACAACAC CATGGAGGAT GCCGGCGCAC TGCCGATCGA GCTGGACGTG TCGAAGATGG AGCACGGCGA TGTGGTCGAG CTGCGTCCGT ACGAAGGCAA GGCGCTGAAG AACGGTGAAG TGATCGCCGA GTTCCAGGTC AAGTCCGAAG TGCTGTTCGA CGAAGTGCGT GCCGGTGGCC GCATTCCGCT GATCATCGGC CGTGGCCTGA CCGGCAAGGC GCGCGAAGCG CTGGGCCTGG CCCCGACCAA CCTGTTCCGC CTGCCGGTGC AGCCGGCCGA CACCGGCAAG GGCTTCTCGC TGGCACAGAA GATGGTCGGC CGCGCCTGTG GCCTGCCGGA AGGCCAGGGC ATGCGCCCGG GCACCTACTG CGAACCGAAG ATGACCTCGG TCGGCTCGCA GGACACCACC GGCCCGATGA CCCGTGACGA GCTGAAGGAC CTGGCCTGCC TGGGCTTCTC GGCCGACCTG GTGATGCAGT CGTTCTGCCA CACCGCCGCC TACCCGAAGC CGGTGGACGT GAAGACCCAC CACACCCTGC CGGAGTTCAT CTCCACCCGT GGCGGCGTTT CGCTGCGCCC GGGCGACGGC GTGATCCACA GCTGGCTCAA CCGCATGCTG CTGCCGGACA CCGTGGGTAC CGGTGGTGAC TCGCACACCC GCTTCCCGGT GGGCATTTCG TTCCCGGCCG GTTCGGGCCT GGTCGCCTTC GCTGCGGCCA CCGGCGTGAT GCCGCTGGAC ATGCCGGAGT CGGTGCTGGT GCGCTTCAAG GGCAAGATGC AGCCGGGCGT GACCCTGCGT GACCTGGTCA ACGCGATCCC GCTGTACGCG ATCAAGTCGG GCCTGCTGAC CGTGGCCAAG GCTGGCAAGA AGAACATCTT CTCCGGCCGC ATCCTGGAAA TCGAAGGCCT GCCGGAGCTG AAGGTCGAAC AGGCGTTCGA ACTGTCCGAC GCCTCGGCCG AGCGTTCGGC GGCCGGTTGC TCGGTGCGCC TGAACAAGGA ACCGATCATC GAGTACCTGA CCAGCAACAT CACGCTGCTG AAGTGGATGA TTGCCGAGGG CTACCAGGAT CCGCGTTCGC TGCAGCGTCG CATCGAGAAG ATGGAGGCGT GGCTGGCCAA CCCGCAGCTG CTGGAGCCGG ATGCCGACGC CGAGTACGCC GCCGTCATCG AGATCGACCT GGCCGACATC CACGAGCCGA TCGTGGCCTG CCCGAACGAT CCGGACGACG TGAAGACCCT GTCCGAAGTC GCTGGCGCCA AGATCGACGA AGTGTTCATC GGTTCGTGCA TGACCAACAT CGGTCACTTC CGTGCCGCTG CCAAGCTGCT GGAAGGCAAG CGTGACCTGC CGACCCGCCT GTGGGTGGCC CCGCCGACCA AGATGGACGC CTCGGAGCTG ACCAAGGAAG GCGTGTACGG CACCTTCGGC GCCACCGGCG CCCGCATGGA AATGCCGGGC TGCTCGCTGT GCATGGGCAA CCAGGCGCAG ATCCGCGAAG GCTCCACCGC GATGTCGACC TCGACCCGCA ACTTCCCGAA CCGCCTGGGC CGCAACACCA ACGTGTACCT GGGTTCGGCC GAACTGGCCG CGATCTGCTC GCGTCTGGGC CGCATCCCGA CCAAGGAGGA GTACATGGCG GACATCGGCG TGATCAACGC CAATGGTGCG GAGATCTACC GCTACATGAA CTTCGACCAG ATCGAGGAAT ACCAGGACGT GGCCAACACG GTCGCTGCC

Details for StmaA.18854.a.B1.GE43542

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	94kDa
OBSERVED MW:	94kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML EAYRHHVAER AALGIPPLPL SAQQTADVIE LLKNPPQGEA EFLLDLLTHR VPAGVDDAAK VKASYLAAIA LGSEQNPLIS RERATELLGT MLGGYNVAPL VQLLDDTSAG TIAANGLKKT LLVFDAFHDV QEKAKAGNAN AQAVLQSWAD AEWFTSNPEV PQSLTVTVFK VPGETNTDDL SPAPDATTRP DIPMHALAML KNKRDDAPFT PEEDGKRGPI QQILDLKDKG HLVAYVGDVV GTGSSRKSAT NSVLWWTGDD IPFIPNKRAG GVCLGSKIAP IFYNTMEDAG ALPIELDVSK MEHGDVVELR PYEGKALKNG EVIAEFQVKS EVLFDEVRAG GRIPLIIGRG LTGKAREALG LAPTNLFRLP VQPADTGKGF SLAQKMVGRA CGLPEGQGMR PGTYCEPKMT SVGSQDTTGP MTRDELKDLA CLGFSADLVM QSFCHTAAYP KPVDVKTHHT LPEFISTRGG VSLRPGDGVI HSWLNRMLLP DTVGTGGDSH TRFPVGISFP AGSGLVAFAA ATGVMPLDMP ESVLVRFKGK MQPGVTLRDL VNAIPLYAIK SGLLTVAKAG KKNIFSGRIL EIEGLPELKV EQAFELSDAS AERSAAGCSV RLNKEPIIEY LTSNITLLKW MIAEGYQDPR SLQRRIEKME AWLANPQLLE PDADAEYAAV IEIDLADIHE PIVACPNDPD DVKTLSEVAG AKIDEVFIGS CMTNIGHFRA AAKLLEGKRD LPTRLWVAPP TKMDASELTK EGVYGTFGAT GARMEMPGCS LCMGNQAQIR EGSTAMSTST RNFPNRLGRN TNVYLGSAEL AAICSRLGRI PTKEEYMADI GVINANGAEI YRYMNFDQIE EYQDVANTVA A

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg aagcctaccg ccaccacgtc gccgagcgcg ctgcgcttgg catcccgccg ctgccgctga gcgcgcagca gacggccgat gtcattgaac tgctgaagaa cccgccgcag ggcgaggccg agttcctgct cgacctgctg acccaccgcg tgccggccgg cgtcgatgac gcagccaagg tcaaggcctc gtacctggcc gcgatcgcgc tgggcagcga gcagaacccg ctgatcagcc gcgagcgcgc caccgaactt ctgggcacca tgctcggcgg ttacaacgtt gcgccgttgg tgcagctgct tgacgacacc agcgccggca ccattgctgc caatggcctg aagaagaccc tgctggtgtt cgacgcgttc cacgacgtgc aggaaaaggc caaggccggc aacgccaacg cccaggccgt gctgcagagc tgggccgatg ccgagtggtt caccagcaac ccggaagtgc cgcagagcct gaccgtcacc gtgttcaagg tgcctggcga gaccaacacc gacgacctgt cgccggcacc ggatgcgacc acccgcccgg acatcccgat gcacgccctg gcgatgctga agaacaagcg cgacgacgcg ccgttcaccc cggaagaaga cggcaagcgc ggcccgatcc agcagatcct cgacctgaag gacaagggcc acctggttgc ctatgtcggc gacgtggtcg gtaccggctc ctcgcgcaag tcggccacca acagcgtgct gtggtggacc ggtgatgaca ttccgttcat cccgaacaag cgcgccggtg gcgtctgcct gggttcgaag atcgccccga tcttctacaa caccatggag gatgccggcg cactgccgat cgagctggac gtgtcgaaga tggagcacgg cgatgtggtc gagctgcgtc cgtacgaagg caaggcgctg aagaacggtg aagtgatcgc cgagttccag gtcaagtccg aagtgctgtt cgacgaagtg cgtgccggtg gccgcattcc gctgatcatc ggccgtggcc tgaccggcaa ggcgcgcgaa gcgctgggcc tggccccgac caacctgttc cgcctgccgg tgcagccggc cgacaccggc aagggcttct cgctggcaca gaagatggtc ggccgcgcct gtggcctgcc ggaaggccag ggcatgcgcc cgggcaccta ctgcgaaccg aagatgacct cggtcggctc gcaggacacc accggcccga tgacccgtga cgagctgaag gacctggcct gcctgggctt ctcggccgac ctggtgatgc agtcgttctg ccacaccgcc gcctacccga agccggtgga cgtgaagacc caccacaccc tgccggagtt catctccacc cgtggcggcg tttcgctgcg cccgggcgac ggcgtgatcc acagctggct caaccgcatg ctgctgccgg acaccgtggg taccggtggt gactcgcaca cccgcttccc ggtgggcatt tcgttcccgg ccggttcggg cctggtcgcc ttcgctgcgg ccaccggcgt gatgccgctg gacatgccgg agtcggtgct ggtgcgcttc aagggcaaga tgcagccggg cgtgaccctg cgtgacctgg tcaacgcgat cccgctgtac gcgatcaagt cgggcctgct gaccgtggcc aaggctggca agaagaacat cttctccggc cgcatcctgg aaatcgaagg cctgccggag ctgaaggtcg aacaggcgtt cgaactgtcc gacgcctcgg ccgagcgttc ggcggccggt tgctcggtgc gcctgaacaa ggaaccgatc atcgagtacc tgaccagcaa catcacgctg ctgaagtgga tgattgccga gggctaccag gatccgcgtt cgctgcagcg tcgcatcgag aagatggagg cgtggctggc caacccgcag ctgctggagc cggatgccga cgccgagtac gccgccgtca tcgagatcga cctggccgac atccacgagc cgatcgtggc ctgcccgaac gatccggacg acgtgaagac cctgtccgaa gtcgctggcg ccaagatcga cgaagtgttc atcggttcgt gcatgaccaa catcggtcac ttccgtgccg ctgccaagct gctggaaggc aagcgtgacc tgccgacccg cctgtgggtg gccccgccga ccaagatgga cgcctcggag ctgaccaagg aaggcgtgta cggcaccttc ggcgccaccg gcgcccgcat ggaaatgccg ggctgctcgc tgtgcatggg caaccaggcg cagatccgcg aaggctccac cgcgatgtcg acctcgaccc gcaacttccc gaaccgcctg ggccgcaaca ccaacgtgta cctgggttcg gccgaactgg ccgcgatctg ctcgcgtctg ggccgcatcc cgaccaagga ggagtacatg gcggacatcg gcgtgatcaa cgccaatggt gcggagatct accgctacat gaacttcgac cagatcgagg aataccagga cgtggccaac acggtcgctg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for StmaA.18854.a.B1.PS38637

PURIFICATION DATe:	2/25/2020
CONCENTRATION:	21.46mg/ml
OBSERVED MW:	94kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGG AAGCCTACCG CCACCACGTC GCCGAGCGCG CTGCGCTTGG CATCCCGCCG CTGCCGCTGA GCGCGCAGCA GACGGCCGAT GTCATTGAAC TGCTGAAGAA CCCGCCGCAG GGCGAGGCCG AGTTCCTGCT CGACCTGCTG ACCCACCGCG TGCCGGCCGG CGTCGATGAC GCAGCCAAGG TCAAGGCCTC GTACCTGGCC GCGATCGCGC TGGGCAGCGA GCAGAACCCG CTGATCAGCC GCGAGCGCGC CACCGAACTT CTGGGCACCA TGCTCGGCGG TTACAACGTT GCGCCGTTGG TGCAGCTGCT TGACGACACC AGCGCCGGCA CCATTGCTGC CAATGGCCTG AAGAAGACCC TGCTGGTGTT CGACGCGTTC CACGACGTGC AGGAAAAGGC CAAGGCCGGC AACGCCAACG CCCAGGCCGT GCTGCAGAGC TGGGCCGATG CCGAGTGGTT CACCAGCAAC CCGGAAGTGC CGCAGAGCCT GACCGTCACC GTGTTCAAGG TGCCTGGCGA GACCAACACC GACGACCTGT CGCCGGCACC GGATGCGACC ACCCGCCCGG ACATCCCGAT GCACGCCCTG GCGATGCTGA AGAACAAGCG CGACGACGCG CCGTTCACCC CGGAAGAAGA CGGCAAGCGC GGCCCGATCC AGCAGATCCT CGACCTGAAG GACAAGGGCC ACCTGGTTGC CTATGTCGGC GACGTGGTCG GTACCGGCTC CTCGCGCAAG TCGGCCACCA ACAGCGTGCT GTGGTGGACC GGTGATGACA TTCCGTTCAT CCCGAACAAG CGCGCCGGTG GCGTCTGCCT GGGTTCGAAG ATCGCCCCGA TCTTCTACAA CACCATGGAG GATGCCGGCG CACTGCCGAT CGAGCTGGAC GTGTCGAAGA TGGAGCACGG CGATGTGGTC GAGCTGCGTC CGTACGAAGG CAAGGCGCTG AAGAACGGTG AAGTGATCGC CGAGTTCCAG GTCAAGTCCG AAGTGCTGTT CGACGAAGTG CGTGCCGGTG GCCGCATTCC GCTGATCATC GGCCGTGGCC TGACCGGCAA GGCGCGCGAA GCGCTGGGCC TGGCCCCGAC CAACCTGTTC CGCCTGCCGG TGCAGCCGGC CGACACCGGC AAGGGCTTCT CGCTGGCACA GAAGATGGTC GGCCGCGCCT GTGGCCTGCC GGAAGGCCAG GGCATGCGCC CGGGCACCTA CTGCGAACCG AAGATGACCT CGGTCGGCTC GCAGGACACC ACCGGCCCGA TGACCCGTGA CGAGCTGAAG GACCTGGCCT GCCTGGGCTT CTCGGCCGAC CTGGTGATGC AGTCGTTCTG CCACACCGCC GCCTACCCGA AGCCGGTGGA CGTGAAGACC CACCACACCC TGCCGGAGTT CATCTCCACC CGTGGCGGCG TTTCGCTGCG CCCGGGCGAC GGCGTGATCC ACAGCTGGCT CAACCGCATG CTGCTGCCGG ACACCGTGGG TACCGGTGGT GACTCGCACA CCCGCTTCCC GGTGGGCATT TCGTTCCCGG CCGGTTCGGG CCTGGTCGCC TTCGCTGCGG CCACCGGCGT GATGCCGCTG GACATGCCGG AGTCGGTGCT GGTGCGCTTC AAGGGCAAGA TGCAGCCGGG CGTGACCCTG CGTGACCTGG TCAACGCGAT CCCGCTGTAC GCGATCAAGT CGGGCCTGCT GACCGTGGCC AAGGCTGGCA AGAAGAACAT CTTCTCCGGC CGCATCCTGG AAATCGAAGG CCTGCCGGAG CTGAAGGTCG AACAGGCGTT CGAACTGTCC GACGCCTCGG CCGAGCGTTC GGCGGCCGGT TGCTCGGTGC GCCTGAACAA GGAACCGATC ATCGAGTACC TGACCAGCAA CATCACGCTG CTGAAGTGGA TGATTGCCGA GGGCTACCAG GATCCGCGTT CGCTGCAGCG TCGCATCGAG AAGATGGAGG CGTGGCTGGC CAACCCGCAG CTGCTGGAGC CGGATGCCGA CGCCGAGTAC GCCGCCGTCA TCGAGATCGA CCTGGCCGAC ATCCACGAGC CGATCGTGGC CTGCCCGAAC GATCCGGACG ACGTGAAGAC CCTGTCCGAA GTCGCTGGCG CCAAGATCGA CGAAGTGTTC ATCGGTTCGT GCATGACCAA CATCGGTCAC TTCCGTGCCG CTGCCAAGCT GCTGGAAGGC AAGCGTGACC TGCCGACCCG CCTGTGGGTG GCCCCGCCGA CCAAGATGGA CGCCTCGGAG CTGACCAAGG AAGGCGTGTA CGGCACCTTC GGCGCCACCG GCGCCCGCAT GGAAATGCCG GGCTGCTCGC TGTGCATGGG CAACCAGGCG CAGATCCGCG AAGGCTCCAC CGCGATGTCG ACCTCGACCC GCAACTTCCC GAACCGCCTG GGCCGCAACA CCAACGTGTA CCTGGGTTCG GCCGAACTGG CCGCGATCTG CTCGCGTCTG GGCCGCATCC CGACCAAGGA GGAGTACATG GCGGACATCG GCGTGATCAA CGCCAATGGT GCGGAGATCT ACCGCTACAT GAACTTCGAC CAGATCGAGG AATACCAGGA CGTGGCCAAC ACGGTCGCTG CCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT