SSGCID: Target Page

StmaA.18849.a

Valyl-tRNA synthetase (EC 6.1.1.9) (valS)

CENTER ID:	StmaA.18849.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.18849.a.B1.GE43540	Full length(StmaA.18849.a)		1	942

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.18849.a.B1.PS38638	Full length(StmaA.18849.a)		1	942

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.643

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQLASSYDP KSFETDLYEA WEKAGHFKPS GTGEPYTILL PPPNVTGTLH MGHAFQQTLM DALVRYHRMR GYDTLWQVGT DHAGIATEMV VSRNLALEGK GETRDSLGRE GFIGKVWEWK QQSGDTIERQ MRRLGTSADW SRSTFTMDPQ PSAAVNEAFV RWYEQGLIYR GQRLVNWDPV LKTAISDLEV ESAEEDGFLW SIAYTLDEGL SYEHVERDAD GVETLRETRD YLVVATTRPE TLLGDTAVMV HPEDKRYAHL IGKSVVLPLT GRRVPVIADD YVDRAFGTGV VKVTPAHDFN DYEVGVRHSL PMINLFTPVA ALNENAPERF QGLDRYAARK AVLAELEDLG ILVETKAHKL QVPRGDRTGQ VIEPYLTDQW FVKMDDLAKR GLELVEDGSI SFVPPNWINT YRHWMNNIQD WCISRQLWWG HRIPAWFDEA TGSCYVGRSE EEVRAKHNLG SDVVLNQESD VLETWFSSQL WPFSTLGWPN EQAMAERGFD RYLPSSVLIT GFDIIFFWVA RMIMATDNLV GKIPFKDVYF TGLIRDGQGQ KMSKSKGNVL DPLDIIDGIT IDDLVAKRTG GLMQPKMVEK IEKATRKEFP DGIAAHGADA LRFTIAALAT HGRDIKFDMN RAEGYKNFCN KLWNASRFAL MNTEGAAFTG VPRPRTDAER WILARLAATT AEAQGHFAAY RFDLLAQCLY EFAWNAFCDW FLELSKPALN GADAADAEST RHTLLYVLEA LLRLLHPLTP FITEQLWQQL APRLGLAETT LSLRPYPTAA EFEGDFAQAE ADVEWLKAVI SAVRRVRSEL NVAPSKLVPL RLQAGLEQDR VRIERFSASL SFLLKLDSIQ WLAEGESAPP AAAAIVGELK LLVPLEGLVD LDAERVRLDK EIARVEAEKE KSETKLAKFT DKVPPAVVEQ ERVRLADWNT QLAGLREQRA KL

NT Sequence

ATGACCCAAC TCGCCTCCAG CTACGACCCG AAGTCCTTCG AGACCGACCT CTACGAGGCC TGGGAGAAGG CCGGCCACTT CAAGCCGTCC GGCACGGGCG AGCCGTACAC CATCCTGCTG CCGCCGCCGA ACGTGACCGG CACGCTGCAC ATGGGCCATG CGTTCCAGCA GACCCTGATG GACGCGCTGG TGCGCTACCA CCGCATGCGC GGCTACGACA CGCTGTGGCA GGTCGGTACC GACCACGCCG GCATCGCCAC CGAAATGGTG GTCAGCCGCA ACCTGGCGCT GGAAGGCAAG GGCGAGACCC GTGATTCGCT GGGCCGCGAA GGCTTCATCG GCAAGGTCTG GGAGTGGAAG CAGCAGTCCG GCGATACCAT CGAGCGCCAG ATGCGCCGCC TCGGCACCTC CGCCGACTGG TCGCGCAGCA CCTTCACCAT GGACCCGCAG CCGTCGGCGG CAGTCAACGA AGCCTTCGTG CGCTGGTACG AGCAGGGCCT GATCTACCGC GGCCAGCGCC TGGTCAACTG GGACCCGGTG CTGAAGACCG CCATTTCCGA CCTGGAAGTG GAGAGCGCCG AGGAAGACGG CTTCCTGTGG TCGATCGCCT ACACGCTGGA TGAGGGCCTG AGCTACGAGC ACGTCGAGCG CGATGCCGAC GGCGTGGAAA CCCTGCGCGA AACCCGCGAC TACCTGGTGG TCGCCACCAC CCGCCCGGAA ACCCTGCTGG GCGATACCGC GGTGATGGTG CACCCGGAAG ACAAGCGCTA CGCGCACCTG ATCGGCAAGA GCGTGGTGCT GCCGCTGACC GGCCGCCGCG TGCCGGTGAT CGCCGACGAC TACGTGGACC GCGCGTTCGG CACCGGCGTG GTCAAGGTCA CCCCAGCGCA TGATTTCAAC GACTACGAAG TGGGCGTGCG CCACAGCCTG CCGATGATCA ACCTGTTCAC CCCGGTGGCA GCGCTGAACG AAAACGCGCC GGAGCGTTTC CAGGGTCTGG ACCGCTACGC CGCGCGCAAG GCCGTGCTGG CCGAACTGGA AGACCTGGGC ATCCTGGTCG AGACCAAGGC GCACAAGCTG CAGGTGCCGC GCGGTGACCG CACCGGCCAG GTGATCGAGC CGTACCTGAC CGACCAGTGG TTCGTGAAGA TGGACGACCT GGCCAAGCGC GGCCTGGAAC TGGTCGAGGA CGGAAGCATT TCCTTCGTGC CGCCGAACTG GATCAACACC TACCGCCACT GGATGAACAA CATCCAGGAC TGGTGCATCA GCCGCCAGCT GTGGTGGGGC CACCGCATTC CGGCGTGGTT CGACGAAGCC ACTGGCAGCT GCTACGTCGG TCGCAGCGAA GAGGAAGTGC GGGCGAAGCA CAACCTGGGC AGCGACGTGG TGCTGAACCA GGAAAGCGAC GTGCTGGAGA CCTGGTTCTC CTCGCAGCTG TGGCCGTTCT CCACCCTGGG CTGGCCCAAC GAGCAGGCCA TGGCCGAGCG CGGCTTTGAT CGCTACCTGC CGTCGTCGGT GCTGATCACC GGCTTCGACA TCATCTTCTT CTGGGTGGCG CGCATGATCA TGGCCACCGA CAACCTGGTC GGGAAAATCC CGTTCAAGGA CGTCTACTTC ACCGGCCTGA TCCGCGACGG CCAGGGCCAG AAGATGTCCA AGAGCAAGGG CAACGTGCTC GACCCGCTGG ACATCATCGA CGGCATCACC ATCGACGACC TGGTCGCCAA GCGCACCGGC GGCCTGATGC AGCCGAAGAT GGTGGAGAAG ATCGAGAAGG CCACCCGCAA GGAATTCCCG GACGGCATCG CCGCCCACGG TGCCGATGCG CTGCGCTTCA CCATCGCCGC GCTGGCCACC CACGGCCGCG ACATCAAGTT CGACATGAAC CGCGCCGAGG GCTACAAGAA CTTCTGCAAC AAGCTGTGGA ATGCCAGCCG CTTCGCGCTG ATGAACACCG AGGGCGCGGC GTTCACCGGC GTTCCCAGGC CGCGTACCGA TGCCGAGCGC TGGATCCTCG CGCGCCTGGC CGCCACCACC GCCGAAGCGC AGGGCCATTT CGCCGCCTAC CGCTTCGACC TGCTGGCGCA GTGCCTGTAC GAGTTCGCCT GGAACGCGTT CTGCGACTGG TTCCTGGAAC TGAGCAAGCC GGCACTGAAC GGCGCAGACG CCGCCGACGC CGAAAGCACC CGCCACACCC TGCTGTACGT GCTGGAAGCG CTGCTGCGCC TGCTGCACCC GCTCACGCCG TTCATCACCG AACAGCTGTG GCAGCAGCTG GCCCCGCGCC TGGGCCTGGC CGAAACCACG CTGTCGCTGC GCCCGTACCC GACCGCCGCC GAGTTCGAAG GCGATTTCGC CCAGGCCGAG GCCGACGTGG AATGGCTGAA GGCGGTGATC AGTGCCGTGC GCCGCGTGCG CAGTGAGCTG AACGTCGCGC CGTCCAAGCT GGTGCCGCTG CGCCTGCAGG CCGGGCTGGA GCAGGACCGC GTGCGCATCG AACGCTTCAG CGCCTCGCTG TCGTTCCTGC TGAAGCTGGA CAGCATCCAG TGGCTGGCCG AAGGCGAAAG CGCACCGCCG GCCGCCGCCG CGATCGTGGG CGAGCTGAAG CTGCTGGTGC CGCTGGAAGG CCTGGTCGAT CTCGATGCCG AGCGCGTGCG CCTGGACAAG GAAATCGCCC GCGTCGAAGC GGAGAAGGAA AAGAGCGAGA CCAAGCTGGC CAAGTTCACC GACAAGGTGC CGCCGGCCGT GGTCGAGCAG GAGCGCGTGC GACTGGCCGA CTGGAACACC CAGCTGGCCG GCCTGCGCGA GCAGCGCGCG AAGCTC

Details for StmaA.18849.a.B1.GE43540

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT QLASSYDPKS FETDLYEAWE KAGHFKPSGT GEPYTILLPP PNVTGTLHMG HAFQQTLMDA LVRYHRMRGY DTLWQVGTDH AGIATEMVVS RNLALEGKGE TRDSLGREGF IGKVWEWKQQ SGDTIERQMR RLGTSADWSR STFTMDPQPS AAVNEAFVRW YEQGLIYRGQ RLVNWDPVLK TAISDLEVES AEEDGFLWSI AYTLDEGLSY EHVERDADGV ETLRETRDYL VVATTRPETL LGDTAVMVHP EDKRYAHLIG KSVVLPLTGR RVPVIADDYV DRAFGTGVVK VTPAHDFNDY EVGVRHSLPM INLFTPVAAL NENAPERFQG LDRYAARKAV LAELEDLGIL VETKAHKLQV PRGDRTGQVI EPYLTDQWFV KMDDLAKRGL ELVEDGSISF VPPNWINTYR HWMNNIQDWC ISRQLWWGHR IPAWFDEATG SCYVGRSEEE VRAKHNLGSD VVLNQESDVL ETWFSSQLWP FSTLGWPNEQ AMAERGFDRY LPSSVLITGF DIIFFWVARM IMATDNLVGK IPFKDVYFTG LIRDGQGQKM SKSKGNVLDP LDIIDGITID DLVAKRTGGL MQPKMVEKIE KATRKEFPDG IAAHGADALR FTIAALATHG RDIKFDMNRA EGYKNFCNKL WNASRFALMN TEGAAFTGVP RPRTDAERWI LARLAATTAE AQGHFAAYRF DLLAQCLYEF AWNAFCDWFL ELSKPALNGA DAADAESTRH TLLYVLEALL RLLHPLTPFI TEQLWQQLAP RLGLAETTLS LRPYPTAAEF EGDFAQAEAD VEWLKAVISA VRRVRSELNV APSKLVPLRL QAGLEQDRVR IERFSASLSF LLKLDSIQWL AEGESAPPAA AAIVGELKLL VPLEGLVDLD AERVRLDKEI ARVEAEKEKS ETKLAKFTDK VPPAVVEQER VRLADWNTQL AGLREQRAKL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc aactcgcctc cagctacgac ccgaagtcct tcgagaccga cctctacgag gcctgggaga aggccggcca cttcaagccg tccggcacgg gcgagccgta caccatcctg ctgccgccgc cgaacgtgac cggcacgctg cacatgggcc atgcgttcca gcagaccctg atggacgcgc tggtgcgcta ccaccgcatg cgcggctacg acacgctgtg gcaggtcggt accgaccacg ccggcatcgc caccgaaatg gtggtcagcc gcaacctggc gctggaaggc aagggcgaga cccgtgattc gctgggccgc gaaggcttca tcggcaaggt ctgggagtgg aagcagcagt ccggcgatac catcgagcgc cagatgcgcc gcctcggcac ctccgccgac tggtcgcgca gcaccttcac catggacccg cagccgtcgg cggcagtcaa cgaagccttc gtgcgctggt acgagcaggg cctgatctac cgcggccagc gcctggtcaa ctgggacccg gtgctgaaga ccgccatttc cgacctggaa gtggagagcg ccgaggaaga cggcttcctg tggtcgatcg cctacacgct ggatgagggc ctgagctacg agcacgtcga gcgcgatgcc gacggcgtgg aaaccctgcg cgaaacccgc gactacctgg tggtcgccac cacccgcccg gaaaccctgc tgggcgatac cgcggtgatg gtgcacccgg aagacaagcg ctacgcgcac ctgatcggca agagcgtggt gctgccgctg accggccgcc gcgtgccggt gatcgccgac gactacgtgg accgcgcgtt cggcaccggc gtggtcaagg tcaccccagc gcatgatttc aacgactacg aagtgggcgt gcgccacagc ctgccgatga tcaacctgtt caccccggtg gcagcgctga acgaaaacgc gccggagcgt ttccagggtc tggaccgcta cgccgcgcgc aaggccgtgc tggccgaact ggaagacctg ggcatcctgg tcgagaccaa ggcgcacaag ctgcaggtgc cgcgcggtga ccgcaccggc caggtgatcg agccgtacct gaccgaccag tggttcgtga agatggacga cctggccaag cgcggcctgg aactggtcga ggacggaagc atttccttcg tgccgccgaa ctggatcaac acctaccgcc actggatgaa caacatccag gactggtgca tcagccgcca gctgtggtgg ggccaccgca ttccggcgtg gttcgacgaa gccactggca gctgctacgt cggtcgcagc gaagaggaag tgcgggcgaa gcacaacctg ggcagcgacg tggtgctgaa ccaggaaagc gacgtgctgg agacctggtt ctcctcgcag ctgtggccgt tctccaccct gggctggccc aacgagcagg ccatggccga gcgcggcttt gatcgctacc tgccgtcgtc ggtgctgatc accggcttcg acatcatctt cttctgggtg gcgcgcatga tcatggccac cgacaacctg gtcgggaaaa tcccgttcaa ggacgtctac ttcaccggcc tgatccgcga cggccagggc cagaagatgt ccaagagcaa gggcaacgtg ctcgacccgc tggacatcat cgacggcatc accatcgacg acctggtcgc caagcgcacc ggcggcctga tgcagccgaa gatggtggag aagatcgaga aggccacccg caaggaattc ccggacggca tcgccgccca cggtgccgat gcgctgcgct tcaccatcgc cgcgctggcc acccacggcc gcgacatcaa gttcgacatg aaccgcgccg agggctacaa gaacttctgc aacaagctgt ggaatgccag ccgcttcgcg ctgatgaaca ccgagggcgc ggcgttcacc ggcgttccca ggccgcgtac cgatgccgag cgctggatcc tcgcgcgcct ggccgccacc accgccgaag cgcagggcca tttcgccgcc taccgcttcg acctgctggc gcagtgcctg tacgagttcg cctggaacgc gttctgcgac tggttcctgg aactgagcaa gccggcactg aacggcgcag acgccgccga cgccgaaagc acccgccaca ccctgctgta cgtgctggaa gcgctgctgc gcctgctgca cccgctcacg ccgttcatca ccgaacagct gtggcagcag ctggccccgc gcctgggcct ggccgaaacc acgctgtcgc tgcgcccgta cccgaccgcc gccgagttcg aaggcgattt cgcccaggcc gaggccgacg tggaatggct gaaggcggtg atcagtgccg tgcgccgcgt gcgcagtgag ctgaacgtcg cgccgtccaa gctggtgccg ctgcgcctgc aggccgggct ggagcaggac cgcgtgcgca tcgaacgctt cagcgcctcg ctgtcgttcc tgctgaagct ggacagcatc cagtggctgg ccgaaggcga aagcgcaccg ccggccgccg ccgcgatcgt gggcgagctg aagctgctgg tgccgctgga aggcctggtc gatctcgatg ccgagcgcgt gcgcctggac aaggaaatcg cccgcgtcga agcggagaag gaaaagagcg agaccaagct ggccaagttc accgacaagg tgccgccggc cgtggtcgag caggagcgcg tgcgactggc cgactggaac acccagctgg ccggcctgcg cgagcagcgc gcgaagctct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for StmaA.18849.a.B1.PS38638

PURIFICATION DATe:	2/25/2020
CONCENTRATION:	23.35mg/ml
OBSERVED MW:	107kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AACTCGCCTC CAGCTACGAC CCGAAGTCCT TCGAGACCGA CCTCTACGAG GCCTGGGAGA AGGCCGGCCA CTTCAAGCCG TCCGGCACGG GCGAGCCGTA CACCATCCTG CTGCCGCCGC CGAACGTGAC CGGCACGCTG CACATGGGCC ATGCGTTCCA GCAGACCCTG ATGGACGCGC TGGTGCGCTA CCACCGCATG CGCGGCTACG ACACGCTGTG GCAGGTCGGT ACCGACCACG CCGGCATCGC CACCGAAATG GTGGTCAGCC GCAACCTGGC GCTGGAAGGC AAGGGCGAGA CCCGTGATTC GCTGGGCCGC GAAGGCTTCA TCGGCAAGGT CTGGGAGTGG AAGCAGCAGT CCGGCGATAC CATCGAGCGC CAGATGCGCC GCCTCGGCAC CTCCGCCGAC TGGTCGCGCA GCACCTTCAC CATGGACCCG CAGCCGTCGG CGGCAGTCAA CGAAGCCTTC GTGCGCTGGT ACGAGCAGGG CCTGATCTAC CGCGGCCAGC GCCTGGTCAA CTGGGACCCG GTGCTGAAGA CCGCCATTTC CGACCTGGAA GTGGAGAGCG CCGAGGAAGA CGGCTTCCTG TGGTCGATCG CCTACACGCT GGATGAGGGC CTGAGCTACG AGCACGTCGA GCGCGATGCC GACGGCGTGG AAACCCTGCG CGAAACCCGC GACTACCTGG TGGTCGCCAC CACCCGCCCG GAAACCCTGC TGGGCGATAC CGCGGTGATG GTGCACCCGG AAGACAAGCG CTACGCGCAC CTGATCGGCA AGAGCGTGGT GCTGCCGCTG ACCGGCCGCC GCGTGCCGGT GATCGCCGAC GACTACGTGG ACCGCGCGTT CGGCACCGGC GTGGTCAAGG TCACCCCAGC GCATGATTTC AACGACTACG AAGTGGGCGT GCGCCACAGC CTGCCGATGA TCAACCTGTT CACCCCGGTG GCAGCGCTGA ACGAAAACGC GCCGGAGCGT TTCCAGGGTC TGGACCGCTA CGCCGCGCGC AAGGCCGTGC TGGCCGAACT GGAAGACCTG GGCATCCTGG TCGAGACCAA GGCGCACAAG CTGCAGGTGC CGCGCGGTGA CCGCACCGGC CAGGTGATCG AGCCGTACCT GACCGACCAG TGGTTCGTGA AGATGGACGA CCTGGCCAAG CGCGGCCTGG AACTGGTCGA GGACGGAAGC ATTTCCTTCG TGCCGCCGAA CTGGATCAAC ACCTACCGCC ACTGGATGAA CAACATCCAG GACTGGTGCA TCAGCCGCCA GCTGTGGTGG GGCCACCGCA TTCCGGCGTG GTTCGACGAA GCCACTGGCA GCTGCTACGT CGGTCGCAGC GAAGAGGAAG TGCGGGCGAA GCACAACCTG GGCAGCGACG TGGTGCTGAA CCAGGAAAGC GACGTGCTGG AGACCTGGTT CTCCTCGCAG CTGTGGCCGT TCTCCACCCT GGGCTGGCCC AACGAGCAGG CCATGGCCGA GCGCGGCTTT GATCGCTACC TGCCGTCGTC GGTGCTGATC ACCGGCTTCG ACATCATCTT CTTCTGGGTG GCGCGCATGA TCATGGCCAC CGACAACCTG GTCGGGAAAA TCCCGTTCAA GGACGTCTAC TTCACCGGCC TGATCCGCGA CGGCCAGGGC CAGAAGATGT CCAAGAGCAA GGGCAACGTG CTCGACCCGC TGGACATCAT CGACGGCATC ACCATCGACG ACCTGGTCGC CAAGCGCACC GGCGGCCTGA TGCAGCCGAA GATGGTGGAG AAGATCGAGA AGGCCACCCG CAAGGAATTC CCGGACGGCA TCGCCGCCCA CGGTGCCGAT GCGCTGCGCT TCACCATCGC CGCGCTGGCC ACCCACGGCC GCGACATCAA GTTCGACATG AACCGCGCCG AGGGCTACAA GAACTTCTGC AACAAGCTGT GGAATGCCAG CCGCTTCGCG CTGATGAACA CCGAGGGCGC GGCGTTCACC GGCGTTCCCA GGCCGCGTAC CGATGCCGAG CGCTGGATCC TCGCGCGCCT GGCCGCCACC ACCGCCGAAG CGCAGGGCCA TTTCGCCGCC TACCGCTTCG ACCTGCTGGC GCAGTGCCTG TACGAGTTCG CCTGGAACGC GTTCTGCGAC TGGTTCCTGG AACTGAGCAA GCCGGCACTG AACGGCGCAG ACGCCGCCGA CGCCGAAAGC ACCCGCCACA CCCTGCTGTA CGTGCTGGAA GCGCTGCTGC GCCTGCTGCA CCCGCTCACG CCGTTCATCA CCGAACAGCT GTGGCAGCAG CTGGCCCCGC GCCTGGGCCT GGCCGAAACC ACGCTGTCGC TGCGCCCGTA CCCGACCGCC GCCGAGTTCG AAGGCGATTT CGCCCAGGCC GAGGCCGACG TGGAATGGCT GAAGGCGGTG ATCAGTGCCG TGCGCCGCGT GCGCAGTGAG CTGAACGTCG CGCCGTCCAA GCTGGTGCCG CTGCGCCTGC AGGCCGGGCT GGAGCAGGAC CGCGTGCGCA TCGAACGCTT CAGCGCCTCG CTGTCGTTCC TGCTGAAGCT GGACAGCATC CAGTGGCTGG CCGAAGGCGA AAGCGCACCG CCGGCCGCCG CCGCGATCGT GGGCGAGCTG AAGCTGCTGG TGCCGCTGGA AGGCCTGGTC GATCTCGATG CCGAGCGCGT GCGCCTGGAC AAGGAAATCG CCCGCGTCGA AGCGGAGAAG GAAAAGAGCG AGACCAAGCT GGCCAAGTTC ACCGACAAGG TGCCGCCGGC CGTGGTCGAG CAGGAGCGCG TGCGACTGGC CGACTGGAAC ACCCAGCTGG CCGGCCTGCG CGAGCAGCGC GCGAAGCTCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT