StmaA.18840.a

LPS-assembly protein LptD @ Organic solvent tolerance protein precursor (ostA)

CENTER ID: StmaA.18840.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18840.a.B2.GE43537 mature protein 23 833
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.790

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRALRLLPL PLSIAISLPA MADEKPLNWG LCPATDVIPA FTDAPTPVPA QDKAAAAAAR EQQPTDIEGD QLLGTTTVPQ YEGNVALRRG DQFVGTDKLS FDTETGNYIA DGNVRYQDGS IRMVAKRAEG NQESDTHKIS DIKYQLVSRR GNGDAESVDL QGAVGQMHRS TYTTCDPSQP VWKLSAPQIE VDNDEGFGTA RNAVLRIGKV PVLWAPYFKF PIDDRRKTGL LFPQLGMSGR NGFDYAQPIY FNLAPNYDDT LTPRYMSRRG LLLDNEFRYL YNGGRGEVLT SFMPNDRLRD RDRGRVMFTG YHNVDSHWQA RANLAWVSDE RYVEDFANRL VGVTASNLQS TVGLYGTGTN WTAGIMADRW QLTDYTLDER ALPYNRQPRL YFNWDKPVRP WLEAGIYAEA VRFTHDDINF KNAVGTGKDL QYTRNGVQWT DGVGVSGGSR LDVKPYVSFP ISGAAWYVTP TLAYRYTAYQ LDRGLVDGVR GIQAQILRSQ GIDPNTATPE QLRGNTSPSR SLPIGSIDAG LFFDRETSIR GKSFLQTLEP RLFYLRTPYR NQDELPIFDT RDFTFSWGQL FRDSRYTGAD RQNDANQLTL AVSTRFIDQT TGKERFSASI GQIQYFDESR VTVTPGGAPV EKGKSAWIAD GNYMINDRWT LGATYQWDPK YKREDLASVR ARYLMPNDGV VNLSYRYRIN AGALPGANKH DRTPLEQADL SFLYPLNARW SLVGRYYYSL QDKEPLEIIG GVQWDSCCLA VRVVGRRYVR NREGEMNNSI QVEFVLKGLS SIGQDTDRTL RRAILGYNRD DLYLVPPSNT GATRDDYDPN LIP
NT Sequence
GTGCGCCGAG CCCTCCGCCT GCTTCCCCTG CCTCTGAGCA TCGCCATCAG CCTGCCGGCG ATGGCCGATG AAAAGCCGCT CAACTGGGGC CTGTGCCCGG CCACCGACGT CATTCCCGCG TTCACTGACG CCCCCACCCC GGTTCCGGCC CAGGACAAGG CTGCCGCCGC GGCCGCCCGC GAGCAGCAGC CGACCGACAT CGAGGGTGAC CAGCTGCTGG GCACCACCAC CGTGCCGCAG TACGAGGGCA ACGTGGCGCT GCGCCGGGGT GACCAGTTCG TCGGTACCGA CAAGCTCAGC TTCGACACCG AGACCGGCAA CTACATCGCC GACGGCAATG TCCGCTACCA GGACGGGTCG ATCCGGATGG TCGCCAAGCG TGCCGAAGGC AACCAGGAAA GCGATACCCA CAAGATCTCC GACATCAAGT ACCAGCTGGT GTCGCGCCGG GGCAACGGCG ATGCCGAGTC GGTCGACCTG CAGGGTGCGG TCGGCCAGAT GCACCGCTCC ACCTACACCA CCTGCGACCC CTCGCAGCCG GTGTGGAAGC TGTCGGCGCC GCAGATCGAA GTGGACAACG ACGAGGGCTT CGGCACCGCG CGCAACGCCG TGCTGCGCAT CGGCAAGGTG CCGGTGCTGT GGGCGCCGTA CTTCAAGTTC CCGATCGATG ACCGCCGCAA GACCGGCCTG CTGTTCCCGC AGCTGGGCAT GTCCGGGCGC AACGGCTTCG ACTACGCCCA GCCGATCTAC TTCAACCTGG CGCCGAACTA CGACGACACG CTGACCCCGC GCTACATGAG CCGGCGCGGC CTGCTGCTCG ACAACGAGTT CCGCTACCTC TACAACGGCG GCCGCGGCGA GGTGTTGACC TCGTTCATGC CCAACGACCG CCTGCGTGAC CGTGACCGCG GCCGGGTGAT GTTCACCGGC TACCACAACG TGGACAGCCA CTGGCAGGCC CGCGCCAACC TGGCCTGGGT CAGCGACGAG CGCTATGTCG AGGATTTCGC CAACCGCCTG GTGGGGGTGA CCGCCTCCAA CCTGCAGAGC ACGGTGGGCC TGTATGGCAC CGGCACGAAC TGGACGGCCG GCATCATGGC CGACCGCTGG CAGCTGACCG ACTACACGCT CGACGAGCGC GCCCTGCCCT ACAACCGCCA GCCGCGCCTG TACTTCAACT GGGACAAGCC GGTCCGGCCC TGGCTGGAAG CCGGCATCTA CGCCGAAGCG GTGCGCTTCA CCCACGACGA CATCAACTTC AAGAACGCCG TCGGCACCGG TAAGGATCTG CAGTACACCC GCAACGGAGT GCAGTGGACC GACGGCGTCG GTGTCAGCGG CGGTTCGCGA TTGGACGTGA AGCCGTATGT GTCATTCCCG ATCAGCGGCG CGGCCTGGTA TGTGACCCCG ACCCTGGCCT ACCGTTACAC CGCCTATCAG CTGGACCGTG GCCTGGTGGA TGGTGTGAGA GGCATCCAGG CCCAGATCCT GCGTTCGCAG GGCATCGACC CGAACACGGC CACGCCCGAA CAGCTGCGCG GCAATACCTC GCCCAGCCGC AGCCTGCCGA TCGGCAGCAT CGACGCCGGC CTGTTCTTCG ATCGCGAAAC CAGCATCCGC GGCAAGTCGT TCCTGCAGAC CCTGGAACCG CGCCTGTTCT ACCTGCGCAC GCCGTACCGC AACCAGGACG AGCTGCCGAT CTTCGACACC CGTGACTTCA CCTTCAGCTG GGGCCAGTTG TTCCGCGACT CGCGCTACAC CGGCGCCGAC CGCCAGAACG ACGCCAACCA GCTGACCCTG GCGGTGAGCA CCCGGTTCAT CGACCAGACC ACCGGCAAGG AACGTTTCTC GGCCTCGATC GGCCAGATCC AGTACTTCGA TGAGTCGCGG GTGACCGTCA CCCCCGGCGG CGCCCCGGTG GAAAAGGGCA AGTCGGCCTG GATCGCCGAC GGCAACTACA TGATCAACGA CCGCTGGACC CTGGGCGCCA CCTACCAGTG GGACCCGAAG TACAAGCGCG AGGATCTGGC CAGCGTCCGT GCCCGCTACC TGATGCCCAA CGACGGCGTG GTCAACCTGA GCTACCGCTA CCGCATCAAT GCCGGTGCTC TGCCCGGTGC CAACAAGCAT GACCGCACCC CGCTGGAGCA GGCCGACCTG TCGTTCCTGT ACCCGCTGAA TGCGCGTTGG AGCCTGGTCG GCCGCTATTA CTACTCGCTG CAGGACAAGG AGCCGCTGGA AATCATCGGC GGTGTCCAGT GGGACAGCTG CTGCCTGGCC GTGCGTGTGG TCGGCCGCCG CTATGTCCGC AATCGCGAAG GCGAGATGAA CAACTCCATC CAGGTGGAGT TCGTCCTGAA GGGCCTGAGC TCCATCGGCC AGGACACGGA CCGCACTCTG CGCCGTGCTA TCCTCGGCTA CAACCGCGAC GACCTCTATC TCGTGCCGCC CAGCAACACC GGGGCGACCC GCGATGACTA CGATCCGAAT TTGATCCCA
Details for StmaA.18840.a.B2.GE43537
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 93kDa
OBSERVED MW: 93kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 34
Validated AA Sequence
MAHHHHHHMD EKPLNWGLCP ATDVIPAFTD APTPVPAQDK AAAAAAREQQ PTDIEGDQLL GTTTVPQYEG NVALRRGDQF VGTDKLSFDT ETGNYIADGN VRYQDGSIRM VAKRAEGNQE SDTHKISDIK YQLVSRRGNG DAESVDLQGA VGQMHRSTYT TCDPSQPVWK LSAPQIEVDN DEGFGTARNA VLRIGKVPVL WAPYFKFPID DRRKTGLLFP QLGMSGRNGF DYAQPIYFNL APNYDDTLTP RYMSRRGPAA RQRVPLPLQR RPRRGVDLVH AQRPPA
Validated NT Sequence
tantacgact cnctataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatg aaaagccgct caactggggc ctgtgcccgg ccaccgacgt cattcccgcg ttcactgacg cccccacccc ggttccggcc caggacaagg ctgccgccgc ggccgcccgc gagcagcagc cgaccgacat cgagggtgac cagctgctgg gcaccaccac cgtgccgcag tacgagggca acgtggcgct gcgccggggt gaccagttcg tcggtaccga caagctcagc ttcgacaccg agaccggcaa ctacatcgcc gacggcaatg tccgctacca ggacgggtcg atccggatgg tcgccaagcg tgccgaaggc aaccaggaaa gcgataccca caagatctcc gacatcaagt accagctggt gtcgcgccgg ggcaacggcg atgccgagtc ggtcgacctg cagggtgcgg tcggccagat gcaccgctcc acctacacca cctgcgaccc ctcgcagccg gtgtggaagc tgtcggcgcc gcagatcgaa gtggacaacg acgagggctt cggcaccgcg cgcaacgccg tgctgcgcat cggcaaggtg ccggtgctgt gggcgccgta cttcaagttc ccgatcgatg accgccgcaa gaccggcctg ctgttcccgc agctgggcat gtccgggcgc aacggcttcg actacgccca gccgatctac ttcaacctgg cgccgaacta cgacgacacg ctgaccccgc gctacatgag ccggcgcggg cctgctgctc gacaacgagt tccgctacct ctacaacggc ggccgcggcg aggtgttgac ctcgttcatg cccaacgacc gcctgcgtga ccgtgaccgc ggncgggtga tgttcaccgg ctaccacaac gtggacagcc actggnaggc ccgcnccaac cnggcctggg ncagcgacna gcgctatnnn tcgaggattt cnccaaccgc cngnnggggg gtgaccgcct ccatgcanag cangnnggnn nngnnnnggn ancngnncga actnnnnnnn gnatcangnn ngacnncngg nngcnnancn nacnna
Expected Protein Sequence
MAHHHHHHDE KPLNWGLCPA TDVIPAFTDA PTPVPAQDKA AAAAAREQQP TDIEGDQLLG TTTVPQYEGN VALRRGDQFV GTDKLSFDTE TGNYIADGNV RYQDGSIRMV AKRAEGNQES DTHKISDIKY QLVSRRGNGD AESVDLQGAV GQMHRSTYTT CDPSQPVWKL SAPQIEVDND EGFGTARNAV LRIGKVPVLW APYFKFPIDD RRKTGLLFPQ LGMSGRNGFD YAQPIYFNLA PNYDDTLTPR YMSRRGLLLD NEFRYLYNGG RGEVLTSFMP NDRLRDRDRG RVMFTGYHNV DSHWQARANL AWVSDERYVE DFANRLVGVT ASNLQSTVGL YGTGTNWTAG IMADRWQLTD YTLDERALPY NRQPRLYFNW DKPVRPWLEA GIYAEAVRFT HDDINFKNAV GTGKDLQYTR NGVQWTDGVG VSGGSRLDVK PYVSFPISGA AWYVTPTLAY RYTAYQLDRG LVDGVRGIQA QILRSQGIDP NTATPEQLRG NTSPSRSLPI GSIDAGLFFD RETSIRGKSF LQTLEPRLFY LRTPYRNQDE LPIFDTRDFT FSWGQLFRDS RYTGADRQND ANQLTLAVST RFIDQTTGKE RFSASIGQIQ YFDESRVTVT PGGAPVEKGK SAWIADGNYM INDRWTLGAT YQWDPKYKRE DLASVRARYL MPNDGVVNLS YRYRINAGAL PGANKHDRTP LEQADLSFLY PLNARWSLVG RYYYSLQDKE PLEIIGGVQW DSCCLAVRVV GRRYVRNREG EMNNSIQVEF VLKGLSSIGQ DTDRTLRRAI LGYNRDDLYL VPPSNTGATR DDYDPNLIP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatgaaa agccgctcaa ctggggcctg tgcccggcca ccgacgtcat tcccgcgttc actgacgccc ccaccccggt tccggcccag gacaaggctg ccgccgcggc cgcccgcgag cagcagccga ccgacatcga gggtgaccag ctgctgggca ccaccaccgt gccgcagtac gagggcaacg tggcgctgcg ccggggtgac cagttcgtcg gtaccgacaa gctcagcttc gacaccgaga ccggcaacta catcgccgac ggcaatgtcc gctaccagga cgggtcgatc cggatggtcg ccaagcgtgc cgaaggcaac caggaaagcg atacccacaa gatctccgac atcaagtacc agctggtgtc gcgccggggc aacggcgatg ccgagtcggt cgacctgcag ggtgcggtcg gccagatgca ccgctccacc tacaccacct gcgacccctc gcagccggtg tggaagctgt cggcgccgca gatcgaagtg gacaacgacg agggcttcgg caccgcgcgc aacgccgtgc tgcgcatcgg caaggtgccg gtgctgtggg cgccgtactt caagttcccg atcgatgacc gccgcaagac cggcctgctg ttcccgcagc tgggcatgtc cgggcgcaac ggcttcgact acgcccagcc gatctacttc aacctggcgc cgaactacga cgacacgctg accccgcgct acatgagccg gcgcggcctg ctgctcgaca acgagttccg ctacctctac aacggcggcc gcggcgaggt gttgacctcg ttcatgccca acgaccgcct gcgtgaccgt gaccgcggcc gggtgatgtt caccggctac cacaacgtgg acagccactg gcaggcccgc gccaacctgg cctgggtcag cgacgagcgc tatgtcgagg atttcgccaa ccgcctggtg ggggtgaccg cctccaacct gcagagcacg gtgggcctgt atggcaccgg cacgaactgg acggccggca tcatggccga ccgctggcag ctgaccgact acacgctcga cgagcgcgcc ctgccctaca accgccagcc gcgcctgtac ttcaactggg acaagccggt ccggccctgg ctggaagccg gcatctacgc cgaagcggtg cgcttcaccc acgacgacat caacttcaag aacgccgtcg gcaccggtaa ggatctgcag tacacccgca acggagtgca gtggaccgac ggcgtcggtg tcagcggcgg ttcgcgattg gacgtgaagc cgtatgtgtc attcccgatc agcggcgcgg cctggtatgt gaccccgacc ctggcctacc gttacaccgc ctatcagctg gaccgtggcc tggtggatgg tgtgagaggc atccaggccc agatcctgcg ttcgcagggc atcgacccga acacggccac gcccgaacag ctgcgcggca atacctcgcc cagccgcagc ctgccgatcg gcagcatcga cgccggcctg ttcttcgatc gcgaaaccag catccgcggc aagtcgttcc tgcagaccct ggaaccgcgc ctgttctacc tgcgcacgcc gtaccgcaac caggacgagc tgccgatctt cgacacccgt gacttcacct tcagctgggg ccagttgttc cgcgactcgc gctacaccgg cgccgaccgc cagaacgacg ccaaccagct gaccctggcg gtgagcaccc ggttcatcga ccagaccacc ggcaaggaac gtttctcggc ctcgatcggc cagatccagt acttcgatga gtcgcgggtg accgtcaccc ccggcggcgc cccggtggaa aagggcaagt cggcctggat cgccgacggc aactacatga tcaacgaccg ctggaccctg ggcgccacct accagtggga cccgaagtac aagcgcgagg atctggccag cgtccgtgcc cgctacctga tgcccaacga cggcgtggtc aacctgagct accgctaccg catcaatgcc ggtgctctgc ccggtgccaa caagcatgac cgcaccccgc tggagcaggc cgacctgtcg ttcctgtacc cgctgaatgc gcgttggagc ctggtcggcc gctattacta ctcgctgcag gacaaggagc cgctggaaat catcggcggt gtccagtggg acagctgctg cctggccgtg cgtgtggtcg gccgccgcta tgtccgcaat cgcgaaggcg agatgaacaa ctccatccag gtggagttcg tcctgaaggg cctgagctcc atcggccagg acacggaccg cactctgcgc cgtgctatcc tcggctacaa ccgcgacgac ctctatctcg tgccgcccag caacaccggg gcgacccgcg atgactacga tccgaatttg atcccatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t