StmaA.18615.a

Outer membrane protein assembly factor YaeT

CENTER ID: StmaA.18615.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18615.a.B2.GE43535 mature protein 27 787
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1435

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRLPNRRLL ALALAAVTGA PALAQAAEPF TVSDIRVDGL QRISSGTVFT YLPVERGETI TDNKVGETIR ALYKTGFFED VQLDRQGSIL VVTVKERPAI NKLTVTGNKD IKSEQLLKGL SDIGLTEGGT FDRLSLDRVT QELRRQYNDR GKYTVEITPT VSPLDRNRVD IAIAIKEGKA AKIRHVNLVG TEKFESKDIL ETWESKEHNW ASWYRRDDQY SKEKLSGDLE KLNSWYLDRG YVDFSIDSTQ VSISPDKRDM FLTAGVTEGA QYKISEIKVS GDTILPQEDV ERMVIQKPGD TFSRALLEFS SDTITNSLSN IGYAFAKVNP IPTTNRAEQT VGINMQVVPG PRVSVRRILF RGNTRTSDEV MRREMRQFEN SWYSQAAIDR SKIRLQRLGY FESVDVETPA VSGSNDQVDV VYNVKETTSG SFVFGLGYSQ SYGMTTSVQL SQNNFLGGGN RVSVEASRSS YLQRYGFSYT NPYFTDDGVS LGYNLSWREL DYSDFNTAQY NSTNGSAQVV FGVPLTETDT VSLMFGIDSN QITTYQGSTP QSIIDYINVL GSRTFHAWRT ELGWARDSRN DYFMPTRGTY QRVDLETTLP GSTIEYYKLN YQISKYWPIM PSLVINTRAE IGYGDSYGSD KTAMVPDENG NLRSVTAKGL PFYENFYAGG TNSVRGFEDN TLGPREVTRG YPEGQPLGGS LKTVGSVEAY FPRLFDSPSA RVSAFVDFGN VYNGADNFKA NELRVSTGVA LLWRAPVGPI SISYAFPLKK EDGDKIERLQ FTFGGQF
NT Sequence
ATGACGCGAC TCCCCAATCG CCGCCTGCTG GCCCTCGCCC TCGCCGCCGT CACCGGCGCT CCCGCCCTGG CCCAGGCAGC CGAGCCCTTC ACTGTCAGCG ACATCCGCGT CGATGGCCTG CAGCGCATCA GCTCGGGCAC GGTGTTCACC TACCTGCCGG TGGAACGGGG CGAGACGATC ACCGACAACA AGGTCGGCGA GACCATCCGC GCGCTGTACA AGACCGGCTT CTTCGAAGAC GTGCAGCTGG ACCGCCAGGG CAGCATCCTG GTGGTGACCG TCAAGGAACG CCCGGCGATC AACAAGCTGA CCGTGACCGG CAACAAGGAC ATCAAGTCCG AGCAGCTGCT CAAGGGCCTG TCCGATATCG GCCTGACCGA GGGCGGCACC TTCGACCGCC TGAGCCTGGA CCGCGTGACC CAGGAACTGC GCCGCCAGTA CAACGACCGC GGCAAGTACA CCGTCGAGAT CACCCCGACC GTGAGCCCGC TGGACCGCAA CCGCGTGGAC ATCGCGATCG CCATCAAGGA AGGCAAGGCG GCCAAGATCC GCCACGTCAA CCTGGTCGGC ACCGAGAAGT TCGAGAGCAA GGACATCCTG GAGACCTGGG AGTCCAAGGA GCACAACTGG GCGTCGTGGT ACCGCCGTGA CGACCAGTAC TCCAAGGAAA AGCTGTCCGG CGACCTGGAA AAGCTCAACT CGTGGTACCT GGACCGCGGC TACGTCGACT TCAGCATCGA TTCCACCCAG GTCTCGATCA GCCCCGACAA GCGCGACATG TTCCTCACCG CGGGCGTGAC CGAGGGTGCG CAGTACAAGA TCTCCGAGAT CAAGGTCAGC GGCGACACCA TCCTTCCGCA GGAGGACGTC GAGCGCATGG TGATCCAGAA GCCGGGCGAC ACGTTCTCGC GTGCCCTGCT GGAATTCAGC TCGGACACCA TCACCAACTC GCTGTCCAAC ATCGGCTACG CGTTCGCCAA GGTGAACCCG ATCCCGACCA CCAACCGTGC CGAACAGACG GTAGGGATCA ACATGCAGGT CGTGCCCGGC CCGCGCGTGT CGGTGCGTCG CATCCTGTTC CGTGGCAACA CCCGCACCTC CGACGAAGTG ATGCGTCGCG AAATGCGCCA GTTCGAGAAC AGCTGGTACT CGCAGGCCGC GATCGACCGC TCCAAGATCC GCCTGCAGCG CCTGGGCTAC TTCGAGTCAG TGGACGTCGA GACCCCGGCC GTCAGCGGCA GCAACGACCA GGTCGACGTC GTCTACAACG TCAAGGAAAC CACCTCCGGC AGCTTCGTGT TCGGCCTGGG CTATTCGCAG TCCTACGGCA TGACCACCTC GGTGCAGCTG TCGCAGAACA ACTTCCTCGG CGGCGGCAAC CGCGTGTCGG TCGAAGCCTC GCGCAGCAGC TACCTGCAGC GCTACGGCTT CAGCTACACC AACCCGTACT TCACCGACGA CGGCGTTTCG CTGGGCTACA ACCTGTCCTG GCGTGAACTG GACTACTCCG ACTTCAACAC CGCGCAGTAC AACAGCACCA ATGGTTCTGC GCAGGTGGTG TTCGGCGTGC CGCTGACCGA GACCGACACC GTCTCGCTGA TGTTCGGCAT CGACAGCAAC CAGATCACCA CGTACCAGGG CTCGACCCCG CAGTCGATCA TCGATTACAT CAACGTGCTG GGCAGCAGGA CGTTCCATGC CTGGCGCACG GAGCTGGGTT GGGCGCGTGA CTCGCGCAAC GACTACTTCA TGCCGACCCG CGGCACCTAT CAGCGTGTTG ACCTGGAAAC CACGCTGCCG GGCTCGACCA TCGAGTACTA CAAGCTGAAC TACCAGATCA GCAAGTACTG GCCGATCATG CCGTCGCTGG TGATCAACAC CCGCGCCGAG ATCGGCTACG GCGACAGCTA CGGCAGTGAC AAGACCGCAA TGGTCCCAGA CGAGAACGGC AACCTGCGCT CGGTCACCGC CAAGGGGCTG CCGTTCTACG AGAACTTCTA CGCCGGTGGT ACCAACTCCG TGCGCGGCTT CGAGGACAAC ACCCTCGGCC CGCGCGAAGT AACCCGCGGT TATCCGGAAG GCCAGCCGCT GGGCGGTTCG CTGAAGACCG TCGGTTCGGT CGAAGCCTAC TTCCCGCGTT TGTTCGACAG CCCGTCGGCC CGCGTGTCGG CCTTCGTCGA CTTCGGCAAC GTCTACAACG GCGCCGATAA CTTCAAGGCC AACGAACTGC GAGTGTCTAC CGGTGTAGCC CTGCTGTGGC GCGCCCCGGT CGGTCCGATC TCGATCAGCT ACGCCTTCCC GCTGAAGAAG GAAGACGGCG ACAAGATCGA ACGTCTGCAA TTCACCTTCG GCGGTCAGTT C
Details for StmaA.18615.a.B2.GE43535
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 86kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAE PFTVSDIRVD GLQRISSGTV FTYLPVERGE TITDNKVGET IRALYKTGFF EDVQLDRQGS ILVVTVKERP AINKLTVTGN KDIKSEQLLK GLSDIGLTEG GTFDRLSLDR VTQELRRQYN DRGKYTVEIT PTVSPLDRNR VDIAIAIKEG KAAKIRHVNL VGTEKFESKD ILETWESKEH NWASWYRRDD QYSKEKLSGD LEKLNSWYLD RGYVDFSIDS TQVSISPDKR DMFLTAGVTE GAQYKISEIK VSGDTILPQE DVERMVIQKP GDTFSRALLE FSSDTITNSL SNIGYAFAKV NPIPTTNRAE QTVGINMQVV PGPRVSVRRI LFRGNTRTSD EVMRREMRQF ENSWYSQAAI DRSKIRLQRL GYFESVDVET PAVSGSNDQV DVVYNVKETT SGSFVFGLGY SQSYGMTTSV QLSQNNFLGG GNRVSVEASR SSYLQRYGFS YTNPYFTDDG VSLGYNLSWR ELDYSDFNTA QYNSTNGSAQ VVFGVPLTET DTVSLMFGID SNQITTYQGS TPQSIIDYIN VLGSRTFHAW RTELGWARDS RNDYFMPTRG TYQRVDLETT LPGSTIEYYK LNYQISKYWP IMPSLVINTR AEIGYGDSYG SDKTAMVPDE NGNLRSVTAK GLPFYENFYA GGTNSVRGFE DNTLGPREVT RGYPEGQPLG GSLKTVGSVE AYFPRLFDSP SARVSAFVDF GNVYNGADNF KANELRVSTG VALLWRAPVG PISISYAFPL KKEDGDKIER LQFTFGGQF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgagc ccttcactgt cagcgacatc cgcgtcgatg gcctgcagcg catcagctcg ggcacggtgt tcacctacct gccggtggaa cggggcgaga cgatcaccga caacaaggtc ggcgagacca tccgcgcgct gtacaagacc ggcttcttcg aagacgtgca gctggaccgc cagggcagca tcctggtggt gaccgtcaag gaacgcccgg cgatcaacaa gctgaccgtg accggcaaca aggacatcaa gtccgagcag ctgctcaagg gcctgtccga tatcggcctg accgagggcg gcaccttcga ccgcctgagc ctggaccgcg tgacccagga actgcgccgc cagtacaacg accgcggcaa gtacaccgtc gagatcaccc cgaccgtgag cccgctggac cgcaaccgcg tggacatcgc gatcgccatc aaggaaggca aggcggccaa gatccgccac gtcaacctgg tcggcaccga gaagttcgag agcaaggaca tcctggagac ctgggagtcc aaggagcaca actgggcgtc gtggtaccgc cgtgacgacc agtactccaa ggaaaagctg tccggcgacc tggaaaagct caactcgtgg tacctggacc gcggctacgt cgacttcagc atcgattcca cccaggtctc gatcagcccc gacaagcgcg acatgttcct caccgcgggc gtgaccgagg gtgcgcagta caagatctcc gagatcaagg tcagcggcga caccatcctt ccgcaggagg acgtcgagcg catggtgatc cagaagccgg gcgacacgtt ctcgcgtgcc ctgctggaat tcagctcgga caccatcacc aactcgctgt ccaacatcgg ctacgcgttc gccaaggtga acccgatccc gaccaccaac cgtgccgaac agacggtagg gatcaacatg caggtcgtgc ccggcccgcg cgtgtcggtg cgtcgcatcc tgttccgtgg caacacccgc acctccgacg aagtgatgcg tcgcgaaatg cgccagttcg agaacagctg gtactcgcag gccgcgatcg accgctccaa gatccgcctg cagcgcctgg gctacttcga gtcagtggac gtcgagaccc cggccgtcag cggcagcaac gaccaggtcg acgtcgtcta caacgtcaag gaaaccacct ccggcagctt cgtgttcggc ctgggctatt cgcagtccta cggcatgacc acctcggtgc agctgtcgca gaacaacttc ctcggcggcg gcaaccgcgt gtcggtcgaa gcctcgcgca gcagctacct gcagcgctac ggcttcagct acaccaaccc gtacttcacc gacgacggcg tttcgctggg ctacaacctg tcctggcgtg aactggacta ctccgacttc aacaccgcgc agtacaacag caccaatggt tctgcgcagg tggtgttcgg cgtgccgctg accgagaccg acaccgtctc gctgatgttc ggcatcgaca gcaaccagat caccacgtac cagggctcga ccccgcagtc gatcatcgat tacatcaacg tgctgggcag caggacgttc catgcctggc gcacggagct gggttgggcg cgtgactcgc gcaacgacta cttcatgccg acccgcggca cctatcagcg tgttgacctg gaaaccacgc tgccgggctc gaccatcgag tactacaagc tgaactacca gatcagcaag tactggccga tcatgccgtc gctggtgatc aacacccgcg ccgagatcgg ctacggcgac agctacggca gtgacaagac cgcaatggtc ccagacgaga acggcaacct gcgctcggtc accgccaagg ggctgccgtt ctacgagaac ttctacgccg gtggtaccaa ctccgtgcgc ggcttcgagg acaacaccct cggcccgcgc gaagtaaccc gcggttatcc ggaaggccag ccgctgggcg gttcgctgaa gaccgtcggt tcggtcgaag cctacttccc gcgtttgttc gacagcccgt cggcccgcgt gtcggccttc gtcgacttcg gcaacgtcta caacggcgcc gataacttca aggccaacga actgcgagtg tctaccggtg tagccctgct gtggcgcgcc ccggtcggtc cgatctcgat cagctacgcc ttcccgctga agaaggaaga cggcgacaag atcgaacgtc tgcaattcac cttcggcggt cagttctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t