StmaA.18161.a
Ribulose-phosphate 3-epimerase (EC 5.1.3.1) (rpe)
| CENTER ID: | StmaA.18161.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.18161.a.B1.GE43530 | Full length(StmaA.18161.a) | 1 | 224 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.18161.a.B1.PS38644 | Full length(StmaA.18161.a) | 1 | 224 |
Structures
7SBJ
DEPOSITED: 9/25/2021
DETERMINATION: XRay
CLONE: StmaA.18161.a.B1.GE43530
PROTEIN: StmaA.18161.a.B1.PS38644
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.4085 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHCLIAPSI LSANFARLGE EVDNVLAAGA DWVHFDVMDN HYVPNLTIGP MVCQALRKHG VTAPIDVHLM VEPVDRIIPD FAEAGATYIS FHPEASRHVH RTIQLIRSLG CKPGIVLNPA TPVDILDWVL DDLDLVLLMS VNPGFGGQAF IPSALDKLKV VRKMIDASGK DIRLEIDGGV KADNIGEIAA AGADTFVAGS AIFNAKTSYQ DVIAQMRANV AAAR
NT Sequence
ATGTCCCACT GCCTCATCGC CCCCTCCATC CTGTCCGCCA ACTTCGCCCG CCTCGGCGAG GAAGTCGACA ACGTCCTCGC CGCTGGCGCG GACTGGGTCC ACTTCGACGT GATGGACAAC CACTACGTGC CGAACCTGAC CATCGGCCCG ATGGTCTGCC AGGCGCTGCG CAAGCACGGC GTGACCGCGC CGATCGACGT GCACCTGATG GTCGAGCCGG TGGACCGCAT CATCCCGGAC TTCGCCGAGG CCGGTGCCAC CTACATCAGC TTCCACCCGG AGGCGAGCCG CCACGTGCAC CGCACCATCC AGCTGATCCG TTCGCTGGGC TGCAAGCCGG GCATCGTGCT CAATCCGGCC ACGCCGGTGG ACATCCTCGA CTGGGTGCTG GATGACCTGG ACCTGGTGCT GCTGATGTCG GTCAACCCGG GCTTCGGCGG CCAGGCCTTC ATCCCCTCGG CGCTGGACAA GCTGAAGGTG GTGCGCAAGA TGATCGATGC CAGCGGCAAG GACATCCGCC TGGAGATCGA CGGCGGCGTG AAGGCCGACA ACATCGGCGA GATCGCGGCT GCCGGTGCCG ACACCTTTGT CGCCGGCTCG GCCATCTTCA ACGCCAAGAC CAGCTACCAG GACGTGATCG CGCAGATGCG CGCCAACGTC GCTGCGGCAC GC
Details for StmaA.18161.a.B1.GE43530
| HARVESTED ON: | 11/19/2019 |
| SEQUENCED ON: | 12/3/2019 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS HCLIAPSILS ANFARLGEEV DNVLAAGADW VHFDVMDNHY VPNLTIGPMV CQALRKHGVT APIDVHLMVE PVDRIIPDFA EAGATYISFH PEASRHVHRT IQLIRSLGCK PGIVLNPATP VDILDWVLDD LDLVLLMSVN PGFGGQAFIP SALDKLKVVR KMIDASGKDI RLEIDGGVKA DNIGEIAAAG ADTFVAGSAI FNAKTSYQDV IAQMRANVAA AR
Validated NT Sequence
atcttactca cttagcgtgc cgcagcgacg ttggcgcgca tctgcgcgat cacgtcctgg tagctggtct tggcgttgaa gatggccgag ccggcgacaa aggtgtcggc accggcagcc gcgatctcgc cgatgttgtc ggccttcacg ccgccgtcga tctccaggcg gatgtccttg ccgctggcat cgatcatctt gcgcaccacc ttcagcttgt ccagcgccga ggggatgaag gcctggccgc cgaagcccgg gttgaccgac atcagcagca ccaggtccag gtcatccagc acccagtcga ggatgtccac cggcgtggcc ggattgagca cgatgcccgg cttgcagccc agcgaacgga tcagctggat ggtgcggtgc acgtggcggc tcgcctccgg gtggaagctg atgtaggtgg caccggcctc ggcgaagtcc gggatgatgc ggtccaccgg ctcgaccatc aggtgcacgt cgatcggcgc ggtcacgccg tgcttgcgca gcgcctggca gaccatcggg ccgatggtca ggttcggcac gtagtggttg tccatcacgt cgaagtggac ccagtccgcg ccagcggcga ggacgttgtc gacttcctcg ccgaggcggg cgaagttggc ggacaggatg gagggggcga tgaggcagtg ggacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS HCLIAPSILS ANFARLGEEV DNVLAAGADW VHFDVMDNHY VPNLTIGPMV CQALRKHGVT APIDVHLMVE PVDRIIPDFA EAGATYISFH PEASRHVHRT IQLIRSLGCK PGIVLNPATP VDILDWVLDD LDLVLLMSVN PGFGGQAFIP SALDKLKVVR KMIDASGKDI RLEIDGGVKA DNIGEIAAAG ADTFVAGSAI FNAKTSYQDV IAQMRANVAA AR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccc actgcctcat cgccccctcc atcctgtccg ccaacttcgc ccgcctcggc gaggaagtcg acaacgtcct cgccgctggc gcggactggg tccacttcga cgtgatggac aaccactacg tgccgaacct gaccatcggc ccgatggtct gccaggcgct gcgcaagcac ggcgtgaccg cgccgatcga cgtgcacctg atggtcgagc cggtggaccg catcatcccg gacttcgccg aggccggtgc cacctacatc agcttccacc cggaggcgag ccgccacgtg caccgcacca tccagctgat ccgttcgctg ggctgcaagc cgggcatcgt gctcaatccg gccacgccgg tggacatcct cgactgggtg ctggatgacc tggacctggt gctgctgatg tcggtcaacc cgggcttcgg cggccaggcc ttcatcccct cggcgctgga caagctgaag gtggtgcgca agatgatcga tgccagcggc aaggacatcc gcctggagat cgacggcggc gtgaaggccg acaacatcgg cgagatcgcg gctgccggtg ccgacacctt tgtcgccggc tcggccatct tcaacgccaa gaccagctac caggacgtga tcgcgcagat gcgcgccaac gtcgctgcgg cacgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for StmaA.18161.a.B1.PS38644
| PURIFICATION DATe: | 9/15/2020 |
| CONCENTRATION: | 18.63mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS HCLIAPSILS ANFARLGEEV DNVLAAGADW VHFDVMDNHY VPNLTIGPMV CQALRKHGVT APIDVHLMVE PVDRIIPDFA EAGATYISFH PEASRHVHRT IQLIRSLGCK PGIVLNPATP VDILDWVLDD LDLVLLMSVN PGFGGQAFIP SALDKLKVVR KMIDASGKDI RLEIDGGVKA DNIGEIAAAG ADTFVAGSAI FNAKTSYQDV IAQMRANVAA AR
Validated NT Sequence
atcttactca cttagcgtgc cgcagcgacg ttggcgcgca tctgcgcgat cacgtcctgg tagctggtct tggcgttgaa gatggccgag ccggcgacaa aggtgtcggc accggcagcc gcgatctcgc cgatgttgtc ggccttcacg ccgccgtcga tctccaggcg gatgtccttg ccgctggcat cgatcatctt gcgcaccacc ttcagcttgt ccagcgccga ggggatgaag gcctggccgc cgaagcccgg gttgaccgac atcagcagca ccaggtccag gtcatccagc acccagtcga ggatgtccac cggcgtggcc ggattgagca cgatgcccgg cttgcagccc agcgaacgga tcagctggat ggtgcggtgc acgtggcggc tcgcctccgg gtggaagctg atgtaggtgg caccggcctc ggcgaagtcc gggatgatgc ggtccaccgg ctcgaccatc aggtgcacgt cgatcggcgc ggtcacgccg tgcttgcgca gcgcctggca gaccatcggg ccgatggtca ggttcggcac gtagtggttg tccatcacgt cgaagtggac ccagtccgcg ccagcggcga ggacgttgtc gacttcctcg ccgaggcggg cgaagttggc ggacaggatg gagggggcga tgaggcagtg ggacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS HCLIAPSILS ANFARLGEEV DNVLAAGADW VHFDVMDNHY VPNLTIGPMV CQALRKHGVT APIDVHLMVE PVDRIIPDFA EAGATYISFH PEASRHVHRT IQLIRSLGCK PGIVLNPATP VDILDWVLDD LDLVLLMSVN PGFGGQAFIP SALDKLKVVR KMIDASGKDI RLEIDGGVKA DNIGEIAAAG ADTFVAGSAI FNAKTSYQDV IAQMRANVAA AR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCC ACTGCCTCAT CGCCCCCTCC ATCCTGTCCG CCAACTTCGC CCGCCTCGGC GAGGAAGTCG ACAACGTCCT CGCCGCTGGC GCGGACTGGG TCCACTTCGA CGTGATGGAC AACCACTACG TGCCGAACCT GACCATCGGC CCGATGGTCT GCCAGGCGCT GCGCAAGCAC GGCGTGACCG CGCCGATCGA CGTGCACCTG ATGGTCGAGC CGGTGGACCG CATCATCCCG GACTTCGCCG AGGCCGGTGC CACCTACATC AGCTTCCACC CGGAGGCGAG CCGCCACGTG CACCGCACCA TCCAGCTGAT CCGTTCGCTG GGCTGCAAGC CGGGCATCGT GCTCAATCCG GCCACGCCGG TGGACATCCT CGACTGGGTG CTGGATGACC TGGACCTGGT GCTGCTGATG TCGGTCAACC CGGGCTTCGG CGGCCAGGCC TTCATCCCCT CGGCGCTGGA CAAGCTGAAG GTGGTGCGCA AGATGATCGA TGCCAGCGGC AAGGACATCC GCCTGGAGAT CGACGGCGGC GTGAAGGCCG ACAACATCGG CGAGATCGCG GCTGCCGGTG CCGACACCTT TGTCGCCGGC TCGGCCATCT TCAACGCCAA GACCAGCTAC CAGGACGTGA TCGCGCAGAT GCGCGCCAAC GTCGCTGCGG CACGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT