StmaA.18149.a

Amidophosphoribosyltransferase (EC 2.4.2.14) (purF)

CENTER ID: StmaA.18149.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18149.a.B1.GE44006 Full length(StmaA.18149.a) 1 488
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.934

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCGIVGIVGN QNVAGQLYDG LTVLQHRGQD AAGIATANGS RLRVQKATGL VRDVFDARTM STLEGSVGIA HVRYPTAGSE GMDEAQPFYV NSPYGIALAH NGNLINTEAL RQQVFEQDRR NVNTDSDSEV LLNVFAYELE QQRQLSPEAA IRAVAGVHRR CKGGYAVVSV VLGLGLVAFR DPHGIRPLVL GKRSHAEGDE YIVASESAAL DVLGFQRVRD VQPGEALVIT ARGELFSEIC AEPAEHTPCI FEYVYFARPD SMIDNVSVHK ARMRMGIKLG EKILRLRPDH DIDTIIPIPD TSRDAALEIS NVLGVKYREG FIKNRYIGRT FIMPGQGERV KSVRRKLNPI HLEFRNRVVL LVDDSIVRGT TSQQIVQMAR DAGARKVYLA SAAPPVRYPN IYGIDMPAAE ELVAHNRTVE EIEAHLGCDW LIYQDLEDME AAVSEGNPAL RNFDSSCFNG HYPTGIEPGY FERIQQLRSD DAKHKRRA
NT Sequence
ATGTGTGGCA TCGTCGGAAT CGTCGGCAAC CAGAACGTCG CCGGGCAGTT GTATGACGGC TTGACCGTCC TCCAGCACCG TGGCCAGGAC GCGGCGGGCA TCGCCACCGC CAACGGCAGC CGCCTGCGCG TGCAGAAGGC CACTGGCCTG GTCCGCGATG TGTTCGATGC CCGCACCATG TCCACCCTGG AAGGCAGCGT CGGCATCGCC CACGTGCGTT ACCCGACTGC CGGTTCGGAA GGCATGGACG AGGCGCAGCC GTTCTACGTC AATTCGCCGT ACGGCATCGC GCTGGCCCAC AACGGCAACC TGATCAATAC CGAGGCGCTG CGCCAGCAGG TGTTCGAACA GGACCGCCGC AACGTCAACA CCGATTCGGA CAGCGAAGTG CTGCTGAACG TGTTCGCCTA CGAACTGGAG CAGCAGCGCC AGCTGAGCCC GGAAGCCGCG ATCCGCGCGG TGGCTGGCGT GCACCGCCGC TGCAAGGGTG GCTATGCAGT GGTCAGCGTG GTGCTGGGCC TGGGCCTGGT CGCCTTCCGC GACCCGCATG GCATCCGTCC GCTGGTGCTG GGCAAGCGCA GCCATGCCGA GGGCGACGAG TACATCGTGG CGTCCGAGTC GGCCGCGCTG GACGTGCTGG GCTTCCAGCG CGTGCGCGAT GTGCAGCCGG GCGAAGCGCT GGTGATCACC GCGCGTGGCG AGCTGTTCTC GGAGATCTGC GCCGAGCCGG CCGAGCACAC CCCGTGCATC TTCGAATACG TGTATTTCGC ACGCCCGGAT TCGATGATCG ACAACGTGTC GGTGCACAAG GCGCGCATGC GCATGGGCAT CAAGCTGGGC GAGAAGATCC TGCGCCTGCG CCCGGACCAC GACATCGACA CCATCATCCC GATCCCGGAT ACCTCGCGCG ACGCTGCGCT TGAGATCTCC AACGTGCTCG GCGTGAAGTA CCGCGAAGGC TTCATCAAGA ACCGCTACAT CGGCCGCACC TTCATCATGC CGGGGCAGGG CGAACGCGTG AAGTCGGTGC GTCGCAAGCT CAACCCGATC CACCTTGAGT TCCGCAACCG CGTGGTGCTG CTGGTGGACG ATTCGATCGT GCGTGGTACC ACCAGCCAGC AGATCGTGCA GATGGCCCGT GACGCGGGCG CGCGCAAGGT CTATCTGGCC AGCGCCGCGC CGCCGGTGCG CTACCCGAAC ATCTACGGCA TCGACATGCC GGCGGCCGAG GAACTGGTCG CGCACAACCG CACCGTGGAA GAGATCGAAG CCCACCTGGG CTGCGACTGG CTGATCTACC AGGACCTGGA AGACATGGAA GCGGCAGTGA GCGAGGGCAA TCCGGCGCTG CGCAACTTCG ATTCGTCCTG CTTCAACGGC CACTACCCGA CCGGCATTGA ACCGGGCTAC TTCGAGCGCA TCCAGCAGCT GCGGTCGGAC GACGCCAAGC ACAAGCGCCG CGCC
Details for StmaA.18149.a.B1.GE44006
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMC GIVGIVGNQN VAGQLYDGLT VLQHRGQDAA GIATANGSRL RVQKATGLVR DVFDARTMST LEGSVGIAHV RYPTAGSEGM DEAQPFYVNS PYGIALAHNG NLINTEALRQ QVFEQDRRNV NTDSDSEVLL NVFAYELEQQ RQLSPEAAIR AVAGVHRRCK GGYAVVSVVL GLGLVAFRDP HGIRPLVLGK RSHAEGDEYI VASESAALDV LGFQRVRDVQ PGEALVITAR GELFSEICAE PAEHTPCIFE YVYFARPDSM IDNVSVHKAR MRMGIKLGEK ILRLRPDHDI DTIIPIPDTS RDAALEISNV LGVKYREGFI KNRYIGRTFI MPGQGERVKS VRRKLNPIHL EFRNRVVLLV DDSIVRGTTS QQIVQMARDA GARKVYLASA APPVRYPNIY GIDMPAAEEL VAHNRTVEEI EAHLGCDWLI YQDLEDMEAA VSEGNPALRN FDSSCFNGHY PTGIEPGYFE RIQQLRSDDA KHKRRA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtg gcatcgtcgg aatcgtcggc aaccagaacg tcgccgggca gttgtatgac ggcttgaccg tcctccagca ccgtggccag gacgcggcgg gcatcgccac cgccaacggc agccgcctgc gcgtgcagaa ggccactggc ctggtccgcg atgtgttcga tgcccgcacc atgtccaccc tggaaggcag cgtcggcatc gcccacgtgc gttacccgac tgccggttcg gaaggcatgg acgaggcgca gccgttctac gtcaattcgc cgtacggcat cgcgctggcc cacaacggca acctgatcaa taccgaggcg ctgcgccagc aggtgttcga acaggaccgc cgcaacgtca acaccgattc ggacagcgaa gtgctgctga acgtgttcgc ctacgaactg gagcagcagc gccagctgag cccggaagcc gcgatccgcg cggtggctgg cgtgcaccgc cgctgcaagg gtggctatgc agtggtcagc gtggtgctgg gcctgggcct ggtcgccttc cgcgacccgc atggcatccg tccgctggtg ctgggcaagc gcagccatgc cgagggcgac gagtacatcg tggcgtccga gtcggccgcg ctggacgtgc tgggcttcca gcgcgtgcgc gatgtgcagc cgggcgaagc gctggtgatc accgcgcgtg gcgagctgtt ctcggagatc tgcgccgagc cggccgagca caccccgtgc atcttcgaat acgtgtattt cgcacgcccg gattcgatga tcgacaacgt gtcggtgcac aaggcgcgca tgcgcatggg catcaagctg ggcgagaaga tcctgcgcct gcgcccggac cacgacatcg acaccatcat cccgatcccg gatacctcgc gcgacgctgc gcttgagatc tccaacgtgc tcggcgtgaa gtaccgcgaa ggcttcatca agaaccgcta catcggccgc accttcatca tgccggggca gggcgaacgc gtgaagtcgg tgcgtcgcaa gctcaacccg atccaccttg agttccgcaa ccgcgtggtg ctgctggtgg acgattcgat cgtgcgtggt accaccagcc agcagatcgt gcagatggcc cgtgacgcgg gcgcgcgcaa ggtctatctg gccagcgccg cgccgccggt gcgctacccg aacatctacg gcatcgacat gccggcggcc gaggaactgg tcgcgcacaa ccgcaccgtg gaagagatcg aagcccacct gggctgcgac tggctgatct accaggacct ggaagacatg gaagcggcag tgagcgaggg caatccggcg ctgcgcaact tcgattcgtc ctgcttcaac ggccactacc cgaccggcat tgaaccgggc tacttcgagc gcatccagca gctgcggtcg gacgacgcca agcacaagcg ccgcgcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at