StmaA.18139.c

Energy-dependent translational throttle protein EttA

CENTER ID: StmaA.18139.c
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18139.c.B1.GE43527 Full length(StmaA.18139.c) 1 554
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.683

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSQYIYTMN RVSKVVPPKR QIIKDISLSF FPGAKIGLLG LNGAGKSTVL KIMAGVDTDF EGEARPQPGI KVGYLAQEPE LDPTKTVREA VEEGVGEVLQ AQAALEAVYA AYAEEGADFD ALAKEQERLE AILAAGDAHT LENQLDVAAD ALRLPPWDAV VGKLSGGEKR RVALCRLLLQ KPDMLLLDEP TNHLDAESVE WLEQFLARYT GTVVAVTHDR YFLDNAAEWI LELDRGRGIP WKGNYTDWLT QKDERLKQED NQEKARQKAI QKELEWSRQN AKGGRTKGKA RLARLEELQS VDYQKRNETN EIFIPPGERL GNAVMEFKNV SKKFGDRLLF DNLSFLVPAG AIVGIIGPNG AGKSTLFKMI TGQEKPDTGE IVVGPTVKLS YVDQSRDALE GNHNVFQEIA GGLDILNING IEIQSRAYIG RFNFKGQDQQ KMVGSLSGGE RGRLHMAKTL LQGGNVLLLD EPSNDLDIET LRALEDALLE FPGNTFVISH DRWFLDRIAT HILAFEGDSH VEFFQGNYRE YEEDKRRRMG DDAAPKRLRF KALK
NT Sequence
ATGTCCTCGC AATACATCTA CACCATGAAC CGCGTGTCCA AGGTGGTCCC GCCCAAGCGG CAGATCATCA AGGACATCTC GCTGTCCTTC TTCCCGGGCG CGAAGATCGG CCTGCTGGGC CTGAACGGCG CCGGCAAGTC CACCGTGCTG AAGATCATGG CCGGCGTGGA TACCGATTTC GAGGGCGAAG CCCGCCCGCA GCCCGGCATC AAGGTCGGCT ACCTGGCGCA GGAACCGGAG CTGGACCCGA CCAAGACCGT GCGTGAAGCG GTCGAGGAAG GCGTGGGCGA AGTGCTGCAG GCACAGGCCG CGCTGGAAGC GGTGTACGCC GCCTACGCCG AGGAAGGTGC CGACTTCGAC GCACTGGCCA AGGAACAGGA GCGCCTCGAG GCGATCCTCG CCGCTGGCGA CGCGCACACG CTGGAAAACC AGCTGGACGT GGCCGCCGAT GCGCTGCGCC TGCCGCCGTG GGATGCCGTG GTCGGCAAGC TGTCCGGCGG TGAGAAGCGC CGCGTGGCGC TGTGCCGCCT GCTGCTGCAG AAGCCGGACA TGCTGCTGCT CGACGAACCG ACCAACCACC TCGACGCCGA GTCGGTGGAA TGGCTGGAGC AGTTCCTGGC GCGCTACACC GGCACCGTGG TGGCCGTTAC CCACGATCGC TACTTCCTGG ACAACGCCGC CGAGTGGATC CTGGAACTGG ACCGCGGCCG CGGCATTCCG TGGAAGGGCA ACTACACCGA CTGGCTGACC CAGAAGGACG AGCGCCTGAA GCAGGAAGAC AACCAGGAAA AGGCTCGCCA GAAGGCGATC CAGAAGGAAC TGGAGTGGTC GCGCCAGAAC GCCAAGGGCG GCCGCACCAA GGGCAAGGCC CGTCTGGCCC GCCTGGAAGA GCTGCAGTCG GTCGATTACC AGAAGCGCAA CGAGACCAAC GAAATCTTCA TCCCGCCGGG CGAGCGCCTG GGCAATGCGG TGATGGAGTT CAAGAACGTC TCCAAGAAGT TCGGCGACCG CCTGCTGTTC GACAACCTGA GCTTCCTGGT GCCGGCCGGT GCCATCGTCG GCATCATCGG CCCGAACGGT GCCGGTAAGT CGACCCTGTT CAAGATGATC ACCGGCCAGG AAAAGCCGGA TACCGGCGAG ATCGTGGTCG GCCCGACCGT GAAGCTGTCC TACGTGGACC AGAGCCGCGA CGCGCTGGAA GGCAACCACA ACGTCTTCCA GGAAATCGCC GGCGGCCTGG ACATCCTCAA CATCAACGGC ATCGAGATCC AGTCGCGCGC CTACATCGGC CGCTTCAACT TCAAGGGCCA GGACCAGCAG AAGATGGTCG GTTCGCTGTC CGGTGGTGAG CGTGGCCGCC TGCACATGGC CAAGACCCTG CTGCAGGGTG GCAACGTGCT GCTGCTCGAC GAACCGTCCA ACGACCTGGA CATCGAAACC CTGCGTGCGC TGGAAGATGC GCTGCTGGAG TTCCCGGGCA ACACCTTCGT CATTTCGCAC GACCGCTGGT TCCTGGATCG CATCGCGACC CACATCCTGG CGTTCGAAGG CGACTCGCAC GTGGAGTTCT TCCAGGGCAA CTACCGCGAG TACGAAGAAG ACAAGCGCCG CCGCATGGGC GACGACGCCG CGCCGAAGCG CCTGCGCTTC AAGGCGCTGA AG
Details for StmaA.18139.c.B1.GE43527
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 63kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMS SQYIYTMNRV SKVVPPKRQI IKDISLSFFP GAKIGLLGLN GAGKSTVLKI MAGVDTDFEG EARPQPGIKV GYLAQEPELD PTKTVREAVE EGVGEVLQAQ AALEAVYAAY AEEGADFDAL AKEQERLEAI LAAGDAHTLE NQLDVAADAL RLPPWDAVVG KLSGGEKRRV ALCRLLLQKP DMLLLDEPTN HLDAESVEWL EQFLARYTGT VVAVTHDRYF LDNAAEWILE LDRGRGIPWX GQLHRLG
Validated NT Sequence
ggcgcttcgg cgcggcgtcg tcgcccatgc ggcggcgctt gtcttcttcg tactcgcggt agttgccctg gaagaactcc acgtgcgagt cgccttcgaa cgccaggatg tgggtcgcga tgcgatccag gaaccagcgg tcgtgcgaaa tgacgaaggt gttgcccggg aactccagca gcgcatcttc cagcgcacgc agggtttcga tgtccaggtc gttggacggt tcgtcgagca gcagcacgtt gccaccctgc agcagggtct tggccatgtg caggcggcca cgctcaccac cggacagcga accgaccatc ttctgctggt cctggccctt gaagttgaag cggccgatgt aggcgcgcga ctggatctcg atgccgttga tgttgaggat gtccaggccg ccggcgattt cctggaagac gttgtggttg ccttccagcg cgtcgcggct ctggtccacg taggacagct tcacggtcgg gccgaccacg atctcgccgg tatccggctt ttcctggccg gtgatcatct tgaacagggt cgacttaccg gcaccgttcg ggccgatgat gccgacgatg gcaccggccg gcaccaggaa gctcangttg tcgaacagca ggcggtcgcc gaacttcttg gagacgttct tgaactccat caccgcattg cccaggcgct cgcccggcgg gatgaagatt tcgttggtct cgttgcgctt ctggtaatcg accgactgca gctcttccag gcgggccaga cgggccttgc ccttggtgcg gccgcccttg gcgttctggc gcgaccactc cagttccttc tggatcgcct tctggcgagc cttttcctgg ttntcttcct gcttcaggcn ctcgtcnttc tgggtcagcc nagtcggtgt agttgcccnt tccacggaat gccgcggccg cggtccagtt ccaggatcca ctcggcggcg ttgtccagga agtagcgatc gtgggtaacg gccaccacgg tgccggtgta gcgcgccagg aactgctcca gccattccac cgactcggcg tcgaggtggt tggtcggttc gtcgagcagc agcatgtccg gcttctgcag cagcaggcgg cacagcgcca cgcggcgctt ctcaccgccg gacagcttgc cgaccacggc atcccacggc ggcaggcgca gcgcatcggc ggccacgtcc agctggtttt ccagcgtgtg cgcgtcgcca gcggcgagga tcgcctcgag gcgctcctgt tccttggcca gtgcgtcgaa gtcggcacct tcctcggcgt aggcggcgta caccgcttcc agcgcggcct gtgcctgcag cacttcgccc acgccttcct cgaccgcttc acgcacggtc ttggtcgggt ccagctccgg ttcctgcgcc aggtagccga ccttgatgcc gggctgcggg cgggcttcgc cctcgaaatc ggtatccacg ccggccatga tcttcagcac ggtggacttg ccggcgccgt tcaggcccag caggccgatc ttcgcgcccg ggaagaagga cagcgagatg tccttgatga tctgccgctt gggcgggacc accttggaca cgcggttcat ggtgtagatg tattgcgagg acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS SQYIYTMNRV SKVVPPKRQI IKDISLSFFP GAKIGLLGLN GAGKSTVLKI MAGVDTDFEG EARPQPGIKV GYLAQEPELD PTKTVREAVE EGVGEVLQAQ AALEAVYAAY AEEGADFDAL AKEQERLEAI LAAGDAHTLE NQLDVAADAL RLPPWDAVVG KLSGGEKRRV ALCRLLLQKP DMLLLDEPTN HLDAESVEWL EQFLARYTGT VVAVTHDRYF LDNAAEWILE LDRGRGIPWK GNYTDWLTQK DERLKQEDNQ EKARQKAIQK ELEWSRQNAK GGRTKGKARL ARLEELQSVD YQKRNETNEI FIPPGERLGN AVMEFKNVSK KFGDRLLFDN LSFLVPAGAI VGIIGPNGAG KSTLFKMITG QEKPDTGEIV VGPTVKLSYV DQSRDALEGN HNVFQEIAGG LDILNINGIE IQSRAYIGRF NFKGQDQQKM VGSLSGGERG RLHMAKTLLQ GGNVLLLDEP SNDLDIETLR ALEDALLEFP GNTFVISHDR WFLDRIATHI LAFEGDSHVE FFQGNYREYE EDKRRRMGDD AAPKRLRFKA LK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct cgcaatacat ctacaccatg aaccgcgtgt ccaaggtggt cccgcccaag cggcagatca tcaaggacat ctcgctgtcc ttcttcccgg gcgcgaagat cggcctgctg ggcctgaacg gcgccggcaa gtccaccgtg ctgaagatca tggccggcgt ggataccgat ttcgagggcg aagcccgccc gcagcccggc atcaaggtcg gctacctggc gcaggaaccg gagctggacc cgaccaagac cgtgcgtgaa gcggtcgagg aaggcgtggg cgaagtgctg caggcacagg ccgcgctgga agcggtgtac gccgcctacg ccgaggaagg tgccgacttc gacgcactgg ccaaggaaca ggagcgcctc gaggcgatcc tcgccgctgg cgacgcgcac acgctggaaa accagctgga cgtggccgcc gatgcgctgc gcctgccgcc gtgggatgcc gtggtcggca agctgtccgg cggtgagaag cgccgcgtgg cgctgtgccg cctgctgctg cagaagccgg acatgctgct gctcgacgaa ccgaccaacc acctcgacgc cgagtcggtg gaatggctgg agcagttcct ggcgcgctac accggcaccg tggtggccgt tacccacgat cgctacttcc tggacaacgc cgccgagtgg atcctggaac tggaccgcgg ccgcggcatt ccgtggaagg gcaactacac cgactggctg acccagaagg acgagcgcct gaagcaggaa gacaaccagg aaaaggctcg ccagaaggcg atccagaagg aactggagtg gtcgcgccag aacgccaagg gcggccgcac caagggcaag gcccgtctgg cccgcctgga agagctgcag tcggtcgatt accagaagcg caacgagacc aacgaaatct tcatcccgcc gggcgagcgc ctgggcaatg cggtgatgga gttcaagaac gtctccaaga agttcggcga ccgcctgctg ttcgacaacc tgagcttcct ggtgccggcc ggtgccatcg tcggcatcat cggcccgaac ggtgccggta agtcgaccct gttcaagatg atcaccggcc aggaaaagcc ggataccggc gagatcgtgg tcggcccgac cgtgaagctg tcctacgtgg accagagccg cgacgcgctg gaaggcaacc acaacgtctt ccaggaaatc gccggcggcc tggacatcct caacatcaac ggcatcgaga tccagtcgcg cgcctacatc ggccgcttca acttcaaggg ccaggaccag cagaagatgg tcggttcgct gtccggtggt gagcgtggcc gcctgcacat ggccaagacc ctgctgcagg gtggcaacgt gctgctgctc gacgaaccgt ccaacgacct ggacatcgaa accctgcgtg cgctggaaga tgcgctgctg gagttcccgg gcaacacctt cgtcatttcg cacgaccgct ggttcctgga tcgcatcgcg acccacatcc tggcgttcga aggcgactcg cacgtggagt tcttccaggg caactaccgc gagtacgaag aagacaagcg ccgccgcatg ggcgacgacg ccgcgccgaa gcgcctgcgc ttcaaggcgc tgaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat