SSGCID: Target Page

StmaA.18139.b

Bis-ABC ATPase YheS

CENTER ID:	StmaA.18139.b
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.18139.b.B1.GE43526	Full length(StmaA.18139.b)		1	621

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3506

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MISLRNFALR RGERLLLSNV DLTLHAGYRV GVVGRNGAGK SSLFAAVKGE LEADKGDVDL PGKVRIASVA QETPSLPDPA LSFVLGGDTD VAAVLAEEAE ATAREDWEAV ANAHTKMAEM GAYDAEARAG KLLHGLGFPA ETHHRAVSSF SGGWRVRLNL ARALMMPSDL LLLDEPTNHL DLDAVYWLEQ WLLKYPGTLL LISHDREFLD NVATHTLHLH GGGAKLYPGG YTDFERQRAE QLRQQQIAHE KEQAERAHLQ SFIDRFKAQA SKAAQAQSRM KRLAKLAGTE AVRAEREFRI EFAPPAKLPF SLIRLNHVEA GYGKDAVILH NVGFGLEAGQ RIGLLGPNGA GKTTLVKTLV GELAPIVGER MAHPDLKIGY FAQHTVESLH EGQSPMEHFR EIAPDAPNQS FRDFLGKWNF PGDRAFEPVD GFSGGERARL ALALIAWQQP NVLLLDEPTN HLDLEMREAL AEALATFEGA IVMVSHDRHL IGLVCDTYWR VADGVVEPFD GDLDAYAAWL RTRPQAQGTK ARMEQVEEPV VVKTAPVAQV VQKKPVNPHK LAAAEAKVAE LEGALAELDR QLADPANYAD ATRMAVLGRD RETAAAQLEK AEAAWMELLE S

NT Sequence

ATGATCTCCC TTCGTAATTT CGCCTTGCGC CGCGGCGAGC GGCTGCTGTT GTCCAATGTC GACCTGACCC TGCACGCCGG TTACAGGGTG GGCGTGGTCG GCCGCAACGG TGCTGGCAAG TCCAGCCTGT TCGCGGCGGT GAAGGGCGAG CTGGAGGCCG ACAAGGGTGA CGTCGACCTG CCCGGCAAGG TCCGTATCGC CAGCGTGGCC CAGGAAACCC CGTCGCTGCC GGACCCGGCG CTGAGCTTTG TGCTGGGCGG GGATACCGAT GTGGCTGCGG TGCTGGCTGA AGAGGCTGAA GCAACCGCGC GCGAAGACTG GGAGGCGGTG GCCAACGCGC ACACGAAGAT GGCCGAGATG GGGGCCTACG ACGCCGAAGC GCGCGCCGGC AAGCTGCTGC ACGGCCTCGG TTTCCCGGCC GAGACCCACC ACCGCGCGGT GTCCTCGTTC TCCGGCGGCT GGCGCGTACG CCTGAACCTG GCCCGCGCGC TGATGATGCC CAGTGACCTG CTGCTGCTGG ACGAACCGAC CAACCACCTG GACCTGGACG CGGTGTACTG GCTGGAGCAG TGGCTGCTGA AGTACCCCGG CACCCTGCTG TTGATCTCGC ATGACCGTGA GTTCCTCGAC AACGTGGCCA CCCACACCCT GCACCTGCAC GGCGGCGGCG CCAAGCTCTA CCCGGGCGGT TATACCGATT TCGAGCGCCA GCGCGCCGAA CAGCTGCGCC AGCAGCAGAT CGCGCACGAG AAGGAACAGG CCGAGCGCGC CCACCTGCAG AGCTTCATCG ACCGCTTCAA GGCACAGGCC AGCAAGGCCG CGCAGGCGCA GAGCCGCATG AAGCGCCTGG CCAAGCTGGC CGGCACCGAA GCAGTGCGCG CCGAGCGCGA GTTCCGCATC GAGTTCGCGC CGCCGGCCAA GCTGCCGTTC TCGCTGATCC GCCTGAACCA TGTCGAGGCC GGCTACGGCA AGGACGCAGT GATCCTGCAC AACGTCGGCT TCGGTCTGGA AGCGGGCCAG CGCATTGGCC TGCTCGGCCC GAACGGTGCC GGCAAGACCA CCCTGGTGAA GACCCTGGTG GGCGAGCTGG CACCGATCGT CGGCGAGCGC ATGGCGCACC CGGACCTGAA GATCGGCTAC TTCGCCCAGC ACACGGTGGA GTCGCTGCAC GAAGGCCAGT CGCCGATGGA GCATTTCCGC GAGATCGCCC CGGACGCACC GAACCAGTCG TTCCGCGACT TCCTCGGCAA GTGGAACTTC CCGGGTGACC GCGCGTTCGA GCCGGTCGAT GGCTTCTCCG GTGGCGAGCG CGCACGCCTG GCGCTGGCGC TGATCGCCTG GCAGCAGCCG AACGTGCTGC TGCTGGACGA ACCGACCAAC CACCTTGACC TGGAAATGCG CGAAGCGCTG GCCGAAGCAT TGGCCACCTT CGAAGGCGCG ATCGTGATGG TCTCTCACGA CCGCCATCTG ATCGGCCTGG TCTGCGATAC CTACTGGCGC GTGGCCGATG GCGTGGTCGA GCCGTTCGAT GGCGACCTGG ATGCCTACGC CGCGTGGCTG CGTACCCGCC CGCAGGCGCA GGGCACCAAG GCGCGCATGG AACAGGTGGA AGAGCCGGTG GTGGTCAAGA CCGCCCCGGT GGCGCAGGTG GTGCAGAAGA AGCCGGTGAA CCCGCACAAG CTGGCCGCCG CTGAAGCCAA GGTGGCCGAG CTGGAAGGTG CCCTGGCCGA GCTGGACCGC CAGCTGGCCG ACCCGGCCAA CTACGCCGAT GCCACGCGCA TGGCCGTGCT TGGCCGCGAT CGCGAAACCG CCGCCGCGCA GCTGGAAAAG GCCGAAGCCG CCTGGATGGA ACTGCTTGAG TCC

Details for StmaA.18139.b.B1.GE43526

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	69kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	53

Validated AA Sequence

MAHHHHHHMI SLRNFALRRG ERLLLSNVDL TLHAGYRVGV VGRNGAGKSS LFAAVKGELE ADKGDVDLPG KVRIASVAQE TPSLPDPALS FVLGGDTDVA AVLAEEAEAT AREDWEAVAN AHTKMAEMGA YDAEARAGKL LHGLGFPAET HHRAVSSFSG GWRVRLNLAR ALMMPSDLLL LDEPTNHLDL EMREALAEAL ATFEGAIVMV SHDRHLIGLV CDTYWRVADG VVEPFDGDLD AYAAWLRTRP QAQGTKARME QVEEPVVVKT APVAQVVQKK PVNPHKLAAA EAKVAELEGA LAELDRQLAD PANYADATRM AVLGRDRETA AAQLEKAEA

Validated NT Sequence

cggcttcggc cttttccagc tgcgcggcgg cggtttcgcg atcgcggcca agcacggcca tgcgcgtggc atcggcgtag ttggccgggt cggccagctg gcggtccagc tcggccaggg caccttccag ctcggccacc ttggcttcag cggcggccag cttgtgcggg ttcaccggct tcttctgcac cacctgcgcc accggggcgg tcttgaccac caccggctct tccacctgtt ccatgcgcgc cttggtgccc tgcgcctgcg ggcgggtacg cagccacgcg gcgtaggcat ccaggtcgcc atcgaacggc tcgaccacgc catcggccac gcgccagtag gtatcgcaga ccaggccgat cagatggcgg tcgtgagaga ccatcacgat cgcgccttcg aaggtggcca atgcttcggc cagcgcttcg cgcatttcca ggtccaggtg gttggtcggt tcgtccagca gcagcaggtc actgggcatc atcagcgcgc gggccaggtt caggcgtacg cgccagccgc cggagaacga ggacaccgcg cggtggtggg tctcggccgg gaaaccgagg ccgtgcagca gcttgccggc gcgcgcttcg gcgtcgtagg cccccatctc ggccatcttc gtgtgcgcgt tggccaccgc ctcccagtct tcgcgcgcgg ttgcttcagc ctcttcagcc agcaccgcag ccacatcggt atccccgccc agcacaaagc tcagcgccgg gtccggcagc gacggggttt cctgggccac gctggcgata cggaccttgc cgggcaggtc gacgtcaccc ttgtcggcct ccagctcgcc cttcaccgcc gcgaacaggc tggacttgcc agcaccgttg cggccgacca cgcccaccct gtaaccggcg tgcagggtca ggtcgacatt ggacaacagc agccgctcgc cgcggcgcaa ggcgaaatta cgaagggaga tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMI SLRNFALRRG ERLLLSNVDL TLHAGYRVGV VGRNGAGKSS LFAAVKGELE ADKGDVDLPG KVRIASVAQE TPSLPDPALS FVLGGDTDVA AVLAEEAEAT AREDWEAVAN AHTKMAEMGA YDAEARAGKL LHGLGFPAET HHRAVSSFSG GWRVRLNLAR ALMMPSDLLL LDEPTNHLDL DAVYWLEQWL LKYPGTLLLI SHDREFLDNV ATHTLHLHGG GAKLYPGGYT DFERQRAEQL RQQQIAHEKE QAERAHLQSF IDRFKAQASK AAQAQSRMKR LAKLAGTEAV RAEREFRIEF APPAKLPFSL IRLNHVEAGY GKDAVILHNV GFGLEAGQRI GLLGPNGAGK TTLVKTLVGE LAPIVGERMA HPDLKIGYFA QHTVESLHEG QSPMEHFREI APDAPNQSFR DFLGKWNFPG DRAFEPVDGF SGGERARLAL ALIAWQQPNV LLLDEPTNHL DLEMREALAE ALATFEGAIV MVSHDRHLIG LVCDTYWRVA DGVVEPFDGD LDAYAAWLRT RPQAQGTKAR MEQVEEPVVV KTAPVAQVVQ KKPVNPHKLA AAEAKVAELE GALAELDRQL ADPANYADAT RMAVLGRDRE TAAAQLEKAE AAWMELLES

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatct cccttcgtaa tttcgccttg cgccgcggcg agcggctgct gttgtccaat gtcgacctga ccctgcacgc cggttacagg gtgggcgtgg tcggccgcaa cggtgctggc aagtccagcc tgttcgcggc ggtgaagggc gagctggagg ccgacaaggg tgacgtcgac ctgcccggca aggtccgtat cgccagcgtg gcccaggaaa ccccgtcgct gccggacccg gcgctgagct ttgtgctggg cggggatacc gatgtggctg cggtgctggc tgaagaggct gaagcaaccg cgcgcgaaga ctgggaggcg gtggccaacg cgcacacgaa gatggccgag atgggggcct acgacgccga agcgcgcgcc ggcaagctgc tgcacggcct cggtttcccg gccgagaccc accaccgcgc ggtgtcctcg ttctccggcg gctggcgcgt acgcctgaac ctggcccgcg cgctgatgat gcccagtgac ctgctgctgc tggacgaacc gaccaaccac ctggacctgg acgcggtgta ctggctggag cagtggctgc tgaagtaccc cggcaccctg ctgttgatct cgcatgaccg tgagttcctc gacaacgtgg ccacccacac cctgcacctg cacggcggcg gcgccaagct ctacccgggc ggttataccg atttcgagcg ccagcgcgcc gaacagctgc gccagcagca gatcgcgcac gagaaggaac aggccgagcg cgcccacctg cagagcttca tcgaccgctt caaggcacag gccagcaagg ccgcgcaggc gcagagccgc atgaagcgcc tggccaagct ggccggcacc gaagcagtgc gcgccgagcg cgagttccgc atcgagttcg cgccgccggc caagctgccg ttctcgctga tccgcctgaa ccatgtcgag gccggctacg gcaaggacgc agtgatcctg cacaacgtcg gcttcggtct ggaagcgggc cagcgcattg gcctgctcgg cccgaacggt gccggcaaga ccaccctggt gaagaccctg gtgggcgagc tggcaccgat cgtcggcgag cgcatggcgc acccggacct gaagatcggc tacttcgccc agcacacggt ggagtcgctg cacgaaggcc agtcgccgat ggagcatttc cgcgagatcg ccccggacgc accgaaccag tcgttccgcg acttcctcgg caagtggaac ttcccgggtg accgcgcgtt cgagccggtc gatggcttct ccggtggcga gcgcgcacgc ctggcgctgg cgctgatcgc ctggcagcag ccgaacgtgc tgctgctgga cgaaccgacc aaccaccttg acctggaaat gcgcgaagcg ctggccgaag cattggccac cttcgaaggc gcgatcgtga tggtctctca cgaccgccat ctgatcggcc tggtctgcga tacctactgg cgcgtggccg atggcgtggt cgagccgttc gatggcgacc tggatgccta cgccgcgtgg ctgcgtaccc gcccgcaggc gcagggcacc aaggcgcgca tggaacaggt ggaagagccg gtggtggtca agaccgcccc ggtggcgcag gtggtgcaga agaagccggt gaacccgcac aagctggccg ccgctgaagc caaggtggcc gagctggaag gtgccctggc cgagctggac cgccagctgg ccgacccggc caactacgcc gatgccacgc gcatggccgt gcttggccgc gatcgcgaaa ccgccgccgc gcagctggaa aaggccgaag ccgcctggat ggaactgctt gagtcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t