StmaA.18013.a
Peptide chain release factor 3 (prfC)
| CENTER ID: | StmaA.18013.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.18013.a.B1.GE43523 | Full length(StmaA.18013.a) | 1 | 534 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.18013.a.B1.PW38802 | Full length(StmaA.18013.a) | 1 | 534 |
Structures
7SIQ
DEPOSITED: 10/14/2021
DETERMINATION: XRay
CLONE: StmaA.18013.a.B1.GE43523
PROTEIN: StmaA.18013.a.B1.PW38802
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3417 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEVANEASR RRTFAIISHP DAGKTTLTEK LLLFGGAIQM AGSVKGRKAA RHATSDWMAL EKERGISVTS SVMQFPYEDK IVNLLDTPGH ADFGEDTYRV LTAVDSALMV IDVAKGVEER TIKLMEVCRL RDTPIMTFIN KLDREGKDPI ELLDEVETVL GIQCAPVTWP IGMGQRLKGV VHLLTGEVHL YEPGRNFTRQ DSTIFPSIDA PGLAEKIGAQ MLADLRDELE LVQGASHPFD LEAYRAGKQT PVFFGSGVNN FGVQPLLDFF VEHAPSPQAR STTGREIAPE ENKLTGFVFK IQANMDPQHR DRVAFMRVCS GRFSAGMKTF HVRTGKEMKL ANALTFMASD REIAAEAWPG DVIGIHNHGT ISIGDTFTEG EAVTFTGIPN FAPELFRRAR LRDPLKLKQL QKGLAQLSEE GATQFFRPLT SNDLILGAVG VLQFDVAAYR LKDEYGVEAT FEPVSVTTAR WVHCSNEKKL EEFREKNALN LALDAAGHLV YLAPTRVNLQ LAQERSPDVR FSATREAAHT VSVG
NT Sequence
ATGTCCGAAG TCGCCAACGA AGCGTCGCGT CGCCGCACCT TCGCCATCAT TTCCCACCCT GACGCCGGCA AGACCACGCT GACCGAAAAG CTGCTGCTGT TCGGAGGCGC GATCCAGATG GCCGGTTCGG TCAAGGGCCG CAAGGCCGCC CGCCACGCCA CCTCCGACTG GATGGCATTG GAAAAGGAAC GCGGCATCTC CGTCACCTCT TCGGTGATGC AGTTCCCGTA CGAAGACAAG ATCGTCAACC TGCTCGACAC CCCCGGCCAC GCCGACTTCG GCGAGGACAC CTACCGCGTG CTGACTGCGG TGGACTCGGC GCTGATGGTG ATCGACGTGG CCAAGGGCGT GGAAGAGCGC ACCATCAAGC TGATGGAAGT GTGCCGCCTG CGCGATACCC CGATCATGAC CTTCATCAAC AAGCTCGACC GCGAGGGCAA GGACCCGATC GAGCTGCTGG ATGAAGTGGA AACCGTGCTG GGCATCCAGT GCGCGCCGGT CACCTGGCCG ATCGGCATGG GCCAGCGCCT GAAGGGCGTG GTCCACCTGC TGACCGGCGA AGTGCACCTG TACGAGCCGG GCCGCAACTT CACCCGCCAG GACTCGACCA TCTTCCCGTC CATCGACGCC CCCGGCCTGG CCGAGAAGAT CGGTGCACAG ATGCTGGCCG ACCTGCGCGA CGAACTGGAA CTGGTGCAGG GTGCCAGCCA CCCGTTCGAC CTGGAGGCCT ACCGCGCCGG CAAGCAGACC CCGGTGTTCT TCGGCTCGGG CGTGAACAAC TTCGGCGTGC AGCCGCTGCT GGACTTCTTC GTCGAGCATG CGCCGTCGCC ACAGGCCCGC TCCACCACCG GCCGCGAGAT CGCCCCGGAA GAAAACAAGC TGACCGGCTT CGTATTCAAG ATCCAGGCCA ACATGGACCC GCAGCACCGC GACCGCGTCG CGTTCATGCG CGTCTGCTCG GGCCGCTTCA GCGCCGGCAT GAAGACCTTC CACGTGCGCA CCGGCAAGGA AATGAAGCTG GCCAACGCAC TGACCTTCAT GGCCAGCGAT CGTGAAATCG CCGCAGAAGC CTGGCCGGGC GATGTGATCG GCATCCACAA CCACGGCACC ATTTCCATCG GCGATACTTT TACCGAAGGC GAAGCGGTCA CCTTCACCGG CATCCCCAAC TTCGCCCCGG AACTGTTCCG CCGCGCGCGC CTGCGCGATC CGCTCAAGCT CAAGCAGCTG CAGAAGGGAC TGGCCCAGCT GTCCGAAGAA GGCGCGACCC AGTTCTTCCG CCCTCTCACC AGCAACGACC TGATCCTGGG TGCGGTGGGT GTGCTGCAGT TCGACGTGGC GGCCTATCGC CTGAAGGACG AGTACGGCGT GGAAGCCACC TTCGAGCCGG TCAGCGTGAC CACCGCGCGT TGGGTCCACT GCAGCAACGA GAAGAAGCTG GAAGAGTTCC GCGAGAAGAA TGCGCTGAAC CTGGCACTGG ATGCAGCCGG CCACCTGGTC TACCTGGCAC CGACCCGGGT CAACCTGCAG CTGGCGCAGG AACGCTCGCC GGACGTGCGT TTCTCGGCCA CCCGCGAAGC GGCGCATACC GTTTCGGTGG GC
Details for StmaA.18013.a.B1.GE43523
| HARVESTED ON: | 11/19/2019 |
| SEQUENCED ON: | 12/3/2019 |
| EXPECTED MW: | 60kDa |
| OBSERVED MW: | 60kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 55 |
Validated AA Sequence
MAHHHHHHMS EVANEASRRR TFAIISHPDA GKTTLTEKLL LFGGAIQMAG SVKGRKAARH ATSDWMALEK ERGISVTSSV MQFPYEDKIV NLLDTPGHAD FGEDTYRVLT AVDSALMVID VAKGVEERTI KLMEVCRLRD TPIMTFINKL DREGKDPIEL LDEVETVLGI QCAPVTWPIG MGQRLKGVVH LLTGEVHLYE PGRNFTRQDS TIFPSIDAPG LAXKIGXQML AXLRDELELX QGASHPFXLX AXRAGKQTPV FFGSGVNNFG VQPLLDFFVX HAPSPQARSX TXPRDRPGXK TS
Validated NT Sequence
tgcgccgctt cgcgggtggc cgagaaacgc acgtccggcg agcgttcctg cgccagctgc aggttgaccc gggtcggtgc caggtagacc aggtggccgg ctgcatccag tgccaggttc agcgcattct tctcgcggaa ctcttccagc ttcttctcgt tgctgcagtg gacccaacgc gcggtggtca cgctgaccgg ctcgaaggtg gcttccacgc cgtactcgtc cttcaggcga taggccgcca cgtcgaactg cagcacaccc accgcaccca ggatcaggtc gttgctggtg agagggcgga agaactgggt cgcgccttct tcggacagct gggccagtcc cttctgcagc tgcttgagct tgagcggatc gcgcaggcgc gcgcggcgga acagttccgg ggcgaagttg gggatgccgg tgaaggtgac cgcttcgcct tcggtaaaag tatcgccgat ggaaatggtg ccgtggttgt ggatgccgat cacatcgccc ggccaggctt ctgcggcgat ttcacgatcg ctggccatga aggtcagtgc gttggccagc ttcatttcct tgccggngcg cacgtggaan gncttcatgc cggcgctgaa gcggcccgag cagacgcgca tgaacgcgac gcggtcgcgg tgctgcgggt ccatgttggc ctggatcttg aatacgaagc cggtcagctt gtttttnttc cggggcgatc tcgcggncnn gtngngganc gggcctgtgg cgacggngca tgctngacga agaagtccag cagcggctgc acgccgaagt tgttcacgcc cganccgaag aacaccgggg tctgcttgcc ggcgcgntag gccnncagnt cgaacgggtg gctggcaccc tgcnccagtt ccagttcgtc gcgcagntcg gccagcatct gtncaccgat cttntcggcc aggccggggg cgtcgatgga cgggaagatg gtcgagtcct ggcgggtgaa gttgcggccc ggctcgtaca ggtgcacttc gccggtcagc aggtggacca cgcccttcag gcgctggccc atgccgatcg gccaggtgac cggcgcgcac tggatgccca gcacggtttc cacttcatcc agcagctcga tcgggtcctt gccctcgcgg tcgagcttgt tgatgaaggt catgatcggg gtatcgcgca ggcggcacac ttccatcagc ttgatggtgc gctcttccac gcccttggcc acgtcgatca ccatcagcgc cgagtccacc gcagtcagca cgcggtaggt gtcctcgccg aagtcggcgt ggccgggggt gtcgagcagg ttgacgatct tgtcttcgta cgggaactgc atcaccgaag aggtgacgga gatgccgcgt tccttttcca atgccatcca gtcggaggtg gcgtggcggg cggccttgcg gcccttgacc gaaccggcca tctggatcgc gcctccgaac agcagcagct tttcggtcag cgtggtcttg ccggcgtcag ggtgggaaat gatggcgaag gtgcggcgac gcgacgcttc gttggcgact tcggacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMS EVANEASRRR TFAIISHPDA GKTTLTEKLL LFGGAIQMAG SVKGRKAARH ATSDWMALEK ERGISVTSSV MQFPYEDKIV NLLDTPGHAD FGEDTYRVLT AVDSALMVID VAKGVEERTI KLMEVCRLRD TPIMTFINKL DREGKDPIEL LDEVETVLGI QCAPVTWPIG MGQRLKGVVH LLTGEVHLYE PGRNFTRQDS TIFPSIDAPG LAEKIGAQML ADLRDELELV QGASHPFDLE AYRAGKQTPV FFGSGVNNFG VQPLLDFFVE HAPSPQARST TGREIAPEEN KLTGFVFKIQ ANMDPQHRDR VAFMRVCSGR FSAGMKTFHV RTGKEMKLAN ALTFMASDRE IAAEAWPGDV IGIHNHGTIS IGDTFTEGEA VTFTGIPNFA PELFRRARLR DPLKLKQLQK GLAQLSEEGA TQFFRPLTSN DLILGAVGVL QFDVAAYRLK DEYGVEATFE PVSVTTARWV HCSNEKKLEE FREKNALNLA LDAAGHLVYL APTRVNLQLA QERSPDVRFS ATREAAHTVS VG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aagtcgccaa cgaagcgtcg cgtcgccgca ccttcgccat catttcccac cctgacgccg gcaagaccac gctgaccgaa aagctgctgc tgttcggagg cgcgatccag atggccggtt cggtcaaggg ccgcaaggcc gcccgccacg ccacctccga ctggatggca ttggaaaagg aacgcggcat ctccgtcacc tcttcggtga tgcagttccc gtacgaagac aagatcgtca acctgctcga cacccccggc cacgccgact tcggcgagga cacctaccgc gtgctgactg cggtggactc ggcgctgatg gtgatcgacg tggccaaggg cgtggaagag cgcaccatca agctgatgga agtgtgccgc ctgcgcgata ccccgatcat gaccttcatc aacaagctcg accgcgaggg caaggacccg atcgagctgc tggatgaagt ggaaaccgtg ctgggcatcc agtgcgcgcc ggtcacctgg ccgatcggca tgggccagcg cctgaagggc gtggtccacc tgctgaccgg cgaagtgcac ctgtacgagc cgggccgcaa cttcacccgc caggactcga ccatcttccc gtccatcgac gcccccggcc tggccgagaa gatcggtgca cagatgctgg ccgacctgcg cgacgaactg gaactggtgc agggtgccag ccacccgttc gacctggagg cctaccgcgc cggcaagcag accccggtgt tcttcggctc gggcgtgaac aacttcggcg tgcagccgct gctggacttc ttcgtcgagc atgcgccgtc gccacaggcc cgctccacca ccggccgcga gatcgccccg gaagaaaaca agctgaccgg cttcgtattc aagatccagg ccaacatgga cccgcagcac cgcgaccgcg tcgcgttcat gcgcgtctgc tcgggccgct tcagcgccgg catgaagacc ttccacgtgc gcaccggcaa ggaaatgaag ctggccaacg cactgacctt catggccagc gatcgtgaaa tcgccgcaga agcctggccg ggcgatgtga tcggcatcca caaccacggc accatttcca tcggcgatac ttttaccgaa ggcgaagcgg tcaccttcac cggcatcccc aacttcgccc cggaactgtt ccgccgcgcg cgcctgcgcg atccgctcaa gctcaagcag ctgcagaagg gactggccca gctgtccgaa gaaggcgcga cccagttctt ccgccctctc accagcaacg acctgatcct gggtgcggtg ggtgtgctgc agttcgacgt ggcggcctat cgcctgaagg acgagtacgg cgtggaagcc accttcgagc cggtcagcgt gaccaccgcg cgttgggtcc actgcagcaa cgagaagaag ctggaagagt tccgcgagaa gaatgcgctg aacctggcac tggatgcagc cggccacctg gtctacctgg caccgacccg ggtcaacctg cagctggcgc aggaacgctc gccggacgtg cgtttctcgg ccacccgcga agcggcgcat accgtttcgg tgggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for StmaA.18013.a.B1.PW38802
| PURIFICATION DATe: | 2/17/2020 |
| CONCENTRATION: | 41mg/ml |
| OBSERVED MW: | 60kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 55 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS EVANEASRRR TFAIISHPDA GKTTLTEKLL LFGGAIQMAG SVKGRKAARH ATSDWMALEK ERGISVTSSV MQFPYEDKIV NLLDTPGHAD FGEDTYRVLT AVDSALMVID VAKGVEERTI KLMEVCRLRD TPIMTFINKL DREGKDPIEL LDEVETVLGI QCAPVTWPIG MGQRLKGVVH LLTGEVHLYE PGRNFTRQDS TIFPSIDAPG LAXKIGXQML AXLRDELELX QGASHPFXLX AXRAGKQTPV FFGSGVNNFG VQPLLDFFVX HAPSPQARSX TXPRDRPGXK TS
Validated NT Sequence
tgcgccgctt cgcgggtggc cgagaaacgc acgtccggcg agcgttcctg cgccagctgc aggttgaccc gggtcggtgc caggtagacc aggtggccgg ctgcatccag tgccaggttc agcgcattct tctcgcggaa ctcttccagc ttcttctcgt tgctgcagtg gacccaacgc gcggtggtca cgctgaccgg ctcgaaggtg gcttccacgc cgtactcgtc cttcaggcga taggccgcca cgtcgaactg cagcacaccc accgcaccca ggatcaggtc gttgctggtg agagggcgga agaactgggt cgcgccttct tcggacagct gggccagtcc cttctgcagc tgcttgagct tgagcggatc gcgcaggcgc gcgcggcgga acagttccgg ggcgaagttg gggatgccgg tgaaggtgac cgcttcgcct tcggtaaaag tatcgccgat ggaaatggtg ccgtggttgt ggatgccgat cacatcgccc ggccaggctt ctgcggcgat ttcacgatcg ctggccatga aggtcagtgc gttggccagc ttcatttcct tgccggngcg cacgtggaan gncttcatgc cggcgctgaa gcggcccgag cagacgcgca tgaacgcgac gcggtcgcgg tgctgcgggt ccatgttggc ctggatcttg aatacgaagc cggtcagctt gtttttnttc cggggcgatc tcgcggncnn gtngngganc gggcctgtgg cgacggngca tgctngacga agaagtccag cagcggctgc acgccgaagt tgttcacgcc cganccgaag aacaccgggg tctgcttgcc ggcgcgntag gccnncagnt cgaacgggtg gctggcaccc tgcnccagtt ccagttcgtc gcgcagntcg gccagcatct gtncaccgat cttntcggcc aggccggggg cgtcgatgga cgggaagatg gtcgagtcct ggcgggtgaa gttgcggccc ggctcgtaca ggtgcacttc gccggtcagc aggtggacca cgcccttcag gcgctggccc atgccgatcg gccaggtgac cggcgcgcac tggatgccca gcacggtttc cacttcatcc agcagctcga tcgggtcctt gccctcgcgg tcgagcttgt tgatgaaggt catgatcggg gtatcgcgca ggcggcacac ttccatcagc ttgatggtgc gctcttccac gcccttggcc acgtcgatca ccatcagcgc cgagtccacc gcagtcagca cgcggtaggt gtcctcgccg aagtcggcgt ggccgggggt gtcgagcagg ttgacgatct tgtcttcgta cgggaactgc atcaccgaag aggtgacgga gatgccgcgt tccttttcca atgccatcca gtcggaggtg gcgtggcggg cggccttgcg gcccttgacc gaaccggcca tctggatcgc gcctccgaac agcagcagct tttcggtcag cgtggtcttg ccggcgtcag ggtgggaaat gatggcgaag gtgcggcgac gcgacgcttc gttggcgact tcggacatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMS EVANEASRRR TFAIISHPDA GKTTLTEKLL LFGGAIQMAG SVKGRKAARH ATSDWMALEK ERGISVTSSV MQFPYEDKIV NLLDTPGHAD FGEDTYRVLT AVDSALMVID VAKGVEERTI KLMEVCRLRD TPIMTFINKL DREGKDPIEL LDEVETVLGI QCAPVTWPIG MGQRLKGVVH LLTGEVHLYE PGRNFTRQDS TIFPSIDAPG LAEKIGAQML ADLRDELELV QGASHPFDLE AYRAGKQTPV FFGSGVNNFG VQPLLDFFVE HAPSPQARST TGREIAPEEN KLTGFVFKIQ ANMDPQHRDR VAFMRVCSGR FSAGMKTFHV RTGKEMKLAN ALTFMASDRE IAAEAWPGDV IGIHNHGTIS IGDTFTEGEA VTFTGIPNFA PELFRRARLR DPLKLKQLQK GLAQLSEEGA TQFFRPLTSN DLILGAVGVL QFDVAAYRLK DEYGVEATFE PVSVTTARWV HCSNEKKLEE FREKNALNLA LDAAGHLVYL APTRVNLQLA QERSPDVRFS ATREAAHTVS VG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG AAGTCGCCAA CGAAGCGTCG CGTCGCCGCA CCTTCGCCAT CATTTCCCAC CCTGACGCCG GCAAGACCAC GCTGACCGAA AAGCTGCTGC TGTTCGGAGG CGCGATCCAG ATGGCCGGTT CGGTCAAGGG CCGCAAGGCC GCCCGCCACG CCACCTCCGA CTGGATGGCA TTGGAAAAGG AACGCGGCAT CTCCGTCACC TCTTCGGTGA TGCAGTTCCC GTACGAAGAC AAGATCGTCA ACCTGCTCGA CACCCCCGGC CACGCCGACT TCGGCGAGGA CACCTACCGC GTGCTGACTG CGGTGGACTC GGCGCTGATG GTGATCGACG TGGCCAAGGG CGTGGAAGAG CGCACCATCA AGCTGATGGA AGTGTGCCGC CTGCGCGATA CCCCGATCAT GACCTTCATC AACAAGCTCG ACCGCGAGGG CAAGGACCCG ATCGAGCTGC TGGATGAAGT GGAAACCGTG CTGGGCATCC AGTGCGCGCC GGTCACCTGG CCGATCGGCA TGGGCCAGCG CCTGAAGGGC GTGGTCCACC TGCTGACCGG CGAAGTGCAC CTGTACGAGC CGGGCCGCAA CTTCACCCGC CAGGACTCGA CCATCTTCCC GTCCATCGAC GCCCCCGGCC TGGCCGAGAA GATCGGTGCA CAGATGCTGG CCGACCTGCG CGACGAACTG GAACTGGTGC AGGGTGCCAG CCACCCGTTC GACCTGGAGG CCTACCGCGC CGGCAAGCAG ACCCCGGTGT TCTTCGGCTC GGGCGTGAAC AACTTCGGCG TGCAGCCGCT GCTGGACTTC TTCGTCGAGC ATGCGCCGTC GCCACAGGCC CGCTCCACCA CCGGCCGCGA GATCGCCCCG GAAGAAAACA AGCTGACCGG CTTCGTATTC AAGATCCAGG CCAACATGGA CCCGCAGCAC CGCGACCGCG TCGCGTTCAT GCGCGTCTGC TCGGGCCGCT TCAGCGCCGG CATGAAGACC TTCCACGTGC GCACCGGCAA GGAAATGAAG CTGGCCAACG CACTGACCTT CATGGCCAGC GATCGTGAAA TCGCCGCAGA AGCCTGGCCG GGCGATGTGA TCGGCATCCA CAACCACGGC ACCATTTCCA TCGGCGATAC TTTTACCGAA GGCGAAGCGG TCACCTTCAC CGGCATCCCC AACTTCGCCC CGGAACTGTT CCGCCGCGCG CGCCTGCGCG ATCCGCTCAA GCTCAAGCAG CTGCAGAAGG GACTGGCCCA GCTGTCCGAA GAAGGCGCGA CCCAGTTCTT CCGCCCTCTC ACCAGCAACG ACCTGATCCT GGGTGCGGTG GGTGTGCTGC AGTTCGACGT GGCGGCCTAT CGCCTGAAGG ACGAGTACGG CGTGGAAGCC ACCTTCGAGC CGGTCAGCGT GACCACCGCG CGTTGGGTCC ACTGCAGCAA CGAGAAGAAG CTGGAAGAGT TCCGCGAGAA GAATGCGCTG AACCTGGCAC TGGATGCAGC CGGCCACCTG GTCTACCTGG CACCGACCCG GGTCAACCTG CAGCTGGCGC AGGAACGCTC GCCGGACGTG CGTTTCTCGG CCACCCGCGA AGCGGCGCAT ACCGTTTCGG TGGGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT