StmaA.18004.a

Succinate dehydrogenase flavoprotein subunit (EC 1.3.5.1) (sdhA)

CENTER ID: StmaA.18004.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.18004.a.B1.GE43522 Full length(StmaA.18004.a) 1 596
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1721

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAYKITEHK YDMVVVGAGG AGLRATFGLA AKGLQTVCLT KVFPTRSHTV AAQGGISAAL ANMGEDDWRY HFFDTIKGSD WLGDQDAIEY MCREAIPAII ELEHYGVPFS RTAEGKIYQR PFGGMTTKYG EGPAAQRTCA AADRTGHAML HTLYQQSLKH DARFMIEYFA LDLIFDDEGA CRGVLALDMS DGTLHLFRAQ GVVLATGGYG RAYFSATSAH TCTGDGGGLA MRAGIAMQDM EFVQFHPTGI YGAGCLITEG VRGEGGILRN SSGERFMERY APHYKDLASR DVVSRSMTIE IREGRGVGEH KDHILLDLTH LGPGVIDDKL PGIAESARIF AGVDVHKQPI PVIPTVHYNM GGIPTNYHGE VVQKVGDNDN AVVPGLYAIG EAACVSVHGA NRLGSNSLLD LVVFGRAVAN RCAETIKPGA SHKPLPADAC DKALGLLDKL RHANGDTPTS VIRDRMQRTM QADAAVFRTS QTLKEGCEKM DKIFDSFQDV KVSDRSLVWN SDLIETYELN NLLLNAVATI NSAEQRKESR GAHAHEDYPD RDDVNWQKHT LVSVDEKGKC SFDYRPVHMY TLTDDVSVVP PKPRVY
NT Sequence
ATGTCCGCTT ACAAGATCAC CGAACACAAG TACGACATGG TCGTGGTCGG CGCCGGCGGC GCCGGCCTGC GCGCCACGTT CGGCCTGGCC GCCAAGGGCC TGCAGACCGT GTGCCTGACC AAGGTCTTCC CGACCCGTTC GCACACCGTT GCCGCCCAGG GCGGCATCTC GGCCGCACTC GCCAACATGG GCGAAGACGA CTGGCGCTAC CACTTCTTCG ACACCATCAA GGGGTCGGAC TGGCTGGGCG ACCAGGACGC CATCGAATAC ATGTGCCGTG AGGCCATCCC GGCCATCATC GAGCTTGAAC ACTACGGCGT GCCGTTCTCG CGCACCGCCG AAGGCAAGAT CTACCAGCGT CCGTTCGGTG GCATGACCAC CAAGTACGGC GAAGGCCCGG CGGCGCAGCG TACCTGCGCG GCGGCCGACC GTACCGGCCA CGCGATGCTG CACACCCTGT ACCAGCAGTC GCTGAAGCAC GATGCGCGCT TCATGATCGA GTACTTCGCG CTCGACCTGA TCTTCGACGA CGAAGGCGCC TGCCGCGGCG TGCTGGCCCT GGACATGTCC GACGGCACCC TGCACCTGTT CCGCGCCCAG GGCGTGGTAC TGGCCACCGG CGGCTACGGC CGTGCCTACT TCAGCGCCAC CTCGGCGCAC ACCTGCACCG GCGACGGTGG CGGCCTGGCC ATGCGTGCCG GCATCGCCAT GCAGGACATG GAGTTCGTGC AGTTCCACCC GACCGGCATC TACGGCGCCG GCTGCCTGAT CACCGAGGGT GTCCGCGGTG AAGGCGGCAT CCTGCGCAAC AGCAGCGGCG AGCGCTTCAT GGAGCGCTAC GCGCCGCACT ACAAGGACCT GGCCTCGCGC GACGTGGTCA GCCGTTCGAT GACCATCGAG ATCCGCGAAG GCCGCGGCGT CGGCGAGCAC AAGGACCACA TCCTGCTCGA CCTGACCCAC CTCGGCCCAG GCGTGATCGA CGACAAGCTG CCGGGCATCG CCGAAAGCGC CCGCATCTTT GCCGGCGTCG ACGTGCACAA GCAGCCGATC CCGGTCATCC CGACCGTGCA CTACAACATG GGCGGCATCC CGACCAACTA CCACGGCGAA GTGGTGCAGA AGGTCGGCGA CAACGACAAT GCCGTGGTGC CGGGCCTGTA CGCCATCGGT GAAGCGGCCT GCGTGTCCGT GCACGGCGCC AACCGACTGG GCTCGAACTC GCTGCTGGAC CTGGTGGTGT TCGGTCGTGC GGTGGCCAAC CGCTGCGCCG AGACCATCAA GCCGGGCGCA TCGCACAAGC CGCTGCCGGC CGATGCCTGC GACAAGGCGC TGGGCCTGCT GGACAAACTG CGCCACGCCA ACGGCGATAC CCCGACCTCG GTCATCCGCG ATCGCATGCA GCGCACCATG CAGGCCGACG CGGCCGTCTT CCGCACCAGC CAGACGCTGA AGGAAGGCTG CGAGAAGATG GACAAGATCT TCGACTCGTT CCAGGACGTG AAGGTCTCCG ACCGTTCGCT GGTGTGGAAC TCGGACCTGA TCGAGACCTA CGAGCTGAAC AACCTGCTGC TCAACGCGGT GGCGACGATC AACTCGGCCG AACAGCGCAA GGAAAGCCGT GGTGCGCATG CGCACGAGGA CTATCCGGAC CGCGACGACG TCAACTGGCA GAAGCACACC CTGGTCAGCG TCGACGAGAA GGGCAAGTGC AGCTTCGACT ACCGTCCGGT GCACATGTAC ACGTTGACCG ACGACGTGTC CGTGGTTCCG CCGAAGCCGC GCGTGTAC
Details for StmaA.18004.a.B1.GE43522
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS AYKITEHKYD MVVVGAGGAG LRATFGLAAK GLQTVCLTKV FPTRSHTVAA QGGISAALAN MGEDDWRYHF FDTIKGSDWL GDQDAIEYMC REAIPAIIEL EHYGVPFSRT AEGKIYQRPF GGMTTKYGEG PAAQRTCAAA DRTGHAMLHT LYQQSLKHDA RFMIEYFALD LIFDDEGACR GVLALDMSDG TLHLFRAQGV VLATGGYGRA YFSATSAHTC TGDGGGLAMR AGIAMQDMEF VQFHPTGIYG AGCLITEGVR GEGGILRNSS GERFMERYAP HYKDLASRDV VSRSMTIEIR EGRGVGEHKD HILLDLTHLG PGVIDDKLPG IAESARIFAG VDVHKQPIPV IPTVHYNMGG IPTNYHGEVV QKVGDNDNAV VPGLYAIGEA ACVSVHGANR LGSNSLLDLV VFGRAVANRC AETIKPGASH KPLPADACDK ALGLLDKLRH ANGDTPTSVI RDRMQRTMQA DAAVFRTSQT LKEGCEKMDK IFDSFQDVKV SDRSLVWNSD LIETYELNNL LLNAVATINS AEQRKESRGA HAHEDYPDRD DVNWQKHTLV SVDEKGKCSF DYRPVHMYTL TDDVSVVPPK PRVY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg cttacaagat caccgaacac aagtacgaca tggtcgtggt cggcgccggc ggcgccggcc tgcgcgccac gttcggcctg gccgccaagg gcctgcagac cgtgtgcctg accaaggtct tcccgacccg ttcgcacacc gttgccgccc agggcggcat ctcggccgca ctcgccaaca tgggcgaaga cgactggcgc taccacttct tcgacaccat caaggggtcg gactggctgg gcgaccagga cgccatcgaa tacatgtgcc gtgaggccat cccggccatc atcgagcttg aacactacgg cgtgccgttc tcgcgcaccg ccgaaggcaa gatctaccag cgtccgttcg gtggcatgac caccaagtac ggcgaaggcc cggcggcgca gcgtacctgc gcggcggccg accgtaccgg ccacgcgatg ctgcacaccc tgtaccagca gtcgctgaag cacgatgcgc gcttcatgat cgagtacttc gcgctcgacc tgatcttcga cgacgaaggc gcctgccgcg gcgtgctggc cctggacatg tccgacggca ccctgcacct gttccgcgcc cagggcgtgg tactggccac cggcggctac ggccgtgcct acttcagcgc cacctcggcg cacacctgca ccggcgacgg tggcggcctg gccatgcgtg ccggcatcgc catgcaggac atggagttcg tgcagttcca cccgaccggc atctacggcg ccggctgcct gatcaccgag ggtgtccgcg gtgaaggcgg catcctgcgc aacagcagcg gcgagcgctt catggagcgc tacgcgccgc actacaagga cctggcctcg cgcgacgtgg tcagccgttc gatgaccatc gagatccgcg aaggccgcgg cgtcggcgag cacaaggacc acatcctgct cgacctgacc cacctcggcc caggcgtgat cgacgacaag ctgccgggca tcgccgaaag cgcccgcatc tttgccggcg tcgacgtgca caagcagccg atcccggtca tcccgaccgt gcactacaac atgggcggca tcccgaccaa ctaccacggc gaagtggtgc agaaggtcgg cgacaacgac aatgccgtgg tgccgggcct gtacgccatc ggtgaagcgg cctgcgtgtc cgtgcacggc gccaaccgac tgggctcgaa ctcgctgctg gacctggtgg tgttcggtcg tgcggtggcc aaccgctgcg ccgagaccat caagccgggc gcatcgcaca agccgctgcc ggccgatgcc tgcgacaagg cgctgggcct gctggacaaa ctgcgccacg ccaacggcga taccccgacc tcggtcatcc gcgatcgcat gcagcgcacc atgcaggccg acgcggccgt cttccgcacc agccagacgc tgaaggaagg ctgcgagaag atggacaaga tcttcgactc gttccaggac gtgaaggtct ccgaccgttc gctggtgtgg aactcggacc tgatcgagac ctacgagctg aacaacctgc tgctcaacgc ggtggcgacg atcaactcgg ccgaacagcg caaggaaagc cgtggtgcgc atgcgcacga ggactatccg gaccgcgacg acgtcaactg gcagaagcac accctggtca gcgtcgacga gaagggcaag tgcagcttcg actaccgtcc ggtgcacatg tacacgttga ccgacgacgt gtccgtggtt ccgccgaagc cgcgcgtgta ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat