StmaA.17999.a

Adenylosuccinate lyase (EC 4.3.2.2) @ SAICAR lyase (EC 4.3.2.2) (purB)

CENTER ID: StmaA.17999.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17999.a.B2.GE43521 mature protein 18 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.2989

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDSALLALS PLDGRYAGKV DALRPIFSEY GLIKARIVVE VEWLLALAAE PGIVELAPFS DAAVARLREL AARFSPAQAA RVKEIERTTN HDVKAVEYFI KEQLKDDAEL APALEFVHFA CTSEDINNLS YGLMLEQARR EVLLPTLDGI ATTLRNLAHA QAGQPMLSRT HGQTASPTTL GKELANVVAR LERQRRQIAA VELTGKINGA VGNYNAHVAS YPDVDWPAFA ERFVTGLGLV FNPYTTQIEP HDNVAEIGDA ARRANIILID LARDIWGYIS LGYFKQRLKE GEVGSSTMPH KVNPIDFENA EGNFGIANAL FEHFSAKLPI SRWQRDLTDS TVLRALGTAF GHSQVALDSL AKGLGKLEVN PQRLDADLDA AWEVLAEAVQ TVMRRHGLPN PYEQLKALTR GQGITAESMR AFVQGLELPA DAKQRLLEMT PGSYTGLAEA LAKKI
NT Sequence
ATGTCCGATT CCGCCCTGCT CGCCCTGTCC CCGCTCGATG GCCGCTACGC CGGCAAGGTC GACGCCCTGC GCCCGATCTT CTCCGAATAC GGCCTGATCA AGGCCCGCAT CGTGGTCGAG GTGGAATGGC TGCTGGCGCT GGCCGCCGAA CCGGGCATCG TCGAACTGGC GCCGTTCTCC GACGCCGCCG TCGCCCGCCT GCGCGAGCTG GCCGCCCGCT TCAGCCCGGC CCAGGCCGCG CGCGTGAAGG AGATCGAGCG CACCACCAAC CATGACGTCA AGGCGGTGGA GTACTTCATC AAGGAACAGC TGAAGGACGA CGCCGAGCTG GCTCCGGCGC TGGAATTCGT GCATTTCGCC TGCACCAGCG AAGACATCAA CAACCTCAGC TACGGCCTGA TGCTGGAACA GGCCCGCCGC GAGGTGCTGC TGCCGACCCT GGACGGCATC GCCACCACGC TGCGCAACCT CGCCCACGCC CAGGCCGGCC AGCCGATGCT GTCGCGCACC CACGGCCAGA CCGCCTCGCC GACCACCCTG GGCAAGGAAC TGGCCAATGT GGTCGCCCGC CTGGAGCGCC AGCGCCGGCA GATCGCTGCG GTCGAGCTGA CCGGCAAGAT CAACGGCGCG GTCGGCAACT ACAACGCCCA CGTGGCCAGC TACCCGGACG TGGACTGGCC GGCCTTCGCC GAGCGCTTCG TGACCGGCCT GGGCCTGGTG TTCAACCCGT ACACCACGCA GATCGAGCCG CACGACAATG TTGCCGAGAT CGGCGATGCT GCGCGCCGCG CCAACATCAT CCTGATCGAC CTGGCCCGCG ACATCTGGGG CTACATCTCG CTGGGCTACT TCAAGCAGCG GCTGAAGGAA GGCGAAGTCG GCTCGTCGAC GATGCCGCAC AAGGTCAATC CGATCGATTT CGAGAATGCG GAAGGCAACT TCGGTATCGC CAACGCGCTG TTCGAGCACT TCAGCGCGAA GCTGCCGATC AGCCGCTGGC AGCGTGACCT GACCGATTCC ACCGTGCTGC GCGCACTGGG CACCGCGTTC GGCCACAGCC AGGTGGCGCT GGATTCGCTG GCCAAGGGCC TGGGCAAGCT GGAAGTGAAC CCGCAGCGCC TGGATGCCGA CCTGGACGCG GCCTGGGAAG TGCTGGCCGA GGCCGTGCAG ACGGTGATGC GCCGCCATGG CCTGCCGAAC CCGTACGAGC AGCTGAAGGC GCTGACCCGT GGCCAGGGCA TCACCGCCGA GTCGATGCGT GCGTTCGTGC AGGGGCTGGA GCTGCCGGCG GATGCGAAGC AGCGCCTGCT GGAGATGACC CCGGGCAGCT ACACCGGCCT GGCCGAGGCC CTGGCGAAGA AGATC
Details for StmaA.17999.a.B2.GE43521
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG KVDALRPIFS EYGLIKARIV VEVEWLLALA AEPGIVELAP FSDAAVARLR ELAARFSPAQ AARVKEIERT TNHDVKAVEY FIKEQLKDDA ELAPALEFVH FACTSEDINN LSYGLMLEQA RREVLLPTLD GIATTLRNLA HAQAGQPMLS RTHGQTASPT TLGKELANVV ARLERQRRQI AAVELTGKIN GAVGNYNAHV ASYPDVDWPA FAERFVTGLG LVFNPYTTQI EPHDNVAEIG DAARRANIIL IDLARDIWGY ISLGYFKQRL KEGEVGSSTM PHKVNPIDFE NAEGNFGIAN ALFEHFSAKL PISRWQRDLT DSTVLRALGT AFGHSQVALD SLAKGLGKLE VNPQRLDADL DAAWEVLAEA VQTVMRRHGL PNPYEQLKAL TRGQGITAES MRAFVQGLEL PADAKQRLLE MTPGSYTGLA
Validated NT Sequence
ctcggccagg ccggtgtagc tgcccggggt catctccagc aggcgctgct tcgcatccgc cggcagctcc agcccctgca cgaacgcacg catcgactcg gcggtgatgc cctggccacg ggtcagcgcc ttcagctgct cgtacgggtt cggcaggcca tggcggcgca tcaccgtctg cacggcctcg gccagcactt cccaggccgc gtccaggtcg gcatccaggc gctgcgggtt cacttccagc ttgcccaggc ccttggccag cgaatccagc gccacctggc tgtggccgaa cgcggtgccc agtgcgcgca gcacggtgga atcggtcagg tcacgctgcc agcggctgat cggcagcttc gcgctgaagt gctcgaacag cgcgttggcg ataccgaagt tgccttccgc attctcgaaa tcgatcggat tgaccttgtg cggcatcgtc gacgagccga cttcgccttc cttcagccgc tgcttgaagt agcccagcga gatgtagccc cagatgtcgc gggccaggtc gatcaggatg atgttggcgc ggcgcgcagc atcgccgatc tcggcaacat tgtcgtgcgg ctcgatctgc gtggtgtacg ggttgaacac caggcccagg ccggtcacga agcgctcggc gaaggccggc cagtccacgt ccgggtagct ggccacgtgg gcgttgtagt tgccgaccgc gccgttgatc ttgccggtca gctcgaccgc agcgatctgc cggcgctggc gctccaggcg ggcgaccaca ttggccagtt ccttgcccag ggtggtcggc gaggcggtct ggccgtgggt gcgcgacagc atcggctggc cggcctgggc gtgggcgagg ttgcgcagcg tggtggcgat gccgtccagg gtcggcagca gcacctcgcg gcgggcctgt tccagcatca ggccgtagct gaggttgttg atgtcttcgc tggtgcaggc gaaatgcacg aattccagcg ccggagccag ctcggcgtcg tccttcagct gttccttgat gaagtactcc accgccttga cgtcatggtt ggtggtgcgc tcgatctcct tcacgcgcgc ggcctgggcc gggctgaagc gggcggccag ctcgcgcagg cgggcgacgg cggcgtcgga gaacggcgcc agttcgacga tgcccggttc ggcggccagc gccagcagcc attccacctc gaccacgatg cgggccttga tcaggccgta ttcggagaag atcgggcgca gggcgtcgac cttgcccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHGK VDALRPIFSE YGLIKARIVV EVEWLLALAA EPGIVELAPF SDAAVARLRE LAARFSPAQA ARVKEIERTT NHDVKAVEYF IKEQLKDDAE LAPALEFVHF ACTSEDINNL SYGLMLEQAR REVLLPTLDG IATTLRNLAH AQAGQPMLSR THGQTASPTT LGKELANVVA RLERQRRQIA AVELTGKING AVGNYNAHVA SYPDVDWPAF AERFVTGLGL VFNPYTTQIE PHDNVAEIGD AARRANIILI DLARDIWGYI SLGYFKQRLK EGEVGSSTMP HKVNPIDFEN AEGNFGIANA LFEHFSAKLP ISRWQRDLTD STVLRALGTA FGHSQVALDS LAKGLGKLEV NPQRLDADLD AAWEVLAEAV QTVMRRHGLP NPYEQLKALT RGQGITAESM RAFVQGLELP ADAKQRLLEM TPGSYTGLAE ALAKKI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcaagg tcgacgccct gcgcccgatc ttctccgaat acggcctgat caaggcccgc atcgtggtcg aggtggaatg gctgctggcg ctggccgccg aaccgggcat cgtcgaactg gcgccgttct ccgacgccgc cgtcgcccgc ctgcgcgagc tggccgcccg cttcagcccg gcccaggccg cgcgcgtgaa ggagatcgag cgcaccacca accatgacgt caaggcggtg gagtacttca tcaaggaaca gctgaaggac gacgccgagc tggctccggc gctggaattc gtgcatttcg cctgcaccag cgaagacatc aacaacctca gctacggcct gatgctggaa caggcccgcc gcgaggtgct gctgccgacc ctggacggca tcgccaccac gctgcgcaac ctcgcccacg cccaggccgg ccagccgatg ctgtcgcgca cccacggcca gaccgcctcg ccgaccaccc tgggcaagga actggccaat gtggtcgccc gcctggagcg ccagcgccgg cagatcgctg cggtcgagct gaccggcaag atcaacggcg cggtcggcaa ctacaacgcc cacgtggcca gctacccgga cgtggactgg ccggccttcg ccgagcgctt cgtgaccggc ctgggcctgg tgttcaaccc gtacaccacg cagatcgagc cgcacgacaa tgttgccgag atcggcgatg ctgcgcgccg cgccaacatc atcctgatcg acctggcccg cgacatctgg ggctacatct cgctgggcta cttcaagcag cggctgaagg aaggcgaagt cggctcgtcg acgatgccgc acaaggtcaa tccgatcgat ttcgagaatg cggaaggcaa cttcggtatc gccaacgcgc tgttcgagca cttcagcgcg aagctgccga tcagccgctg gcagcgtgac ctgaccgatt ccaccgtgct gcgcgcactg ggcaccgcgt tcggccacag ccaggtggcg ctggattcgc tggccaaggg cctgggcaag ctggaagtga acccgcagcg cctggatgcc gacctggacg cggcctggga agtgctggcc gaggccgtgc agacggtgat gcgccgccat ggcctgccga acccgtacga gcagctgaag gcgctgaccc gtggccaggg catcaccgcc gagtcgatgc gtgcgttcgt gcaggggctg gagctgccgg cggatgcgaa gcagcgcctg ctggagatga ccccgggcag ctacaccggc ctggccgagg ccctggcgaa gaagatctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at