StmaA.17994.a

N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18) (dapE)

CENTER ID: StmaA.17994.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17994.a.B1.GE43520 Full length(StmaA.17994.a) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1455

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAARLKQAGF QCDHLRLGDV DNLWATHGLG APVLVLLGHT DVVPTGPRES WTSDPFTPHI RDGVLYGRGA ADMKGSVAAF VVAAEQFVAD HPDHPGTLAV LLTSDEEGDA IDGVRHVARL FAARGQRIDW CITGEPSSTA TLGDLLRVGR RGSLSAKLRV QGVQGHVAYP EKARNPIHQA APALAELCAR RWDDGYESFP PTSLQISNIH AGTGANNVIP GELDVDFNIR YNPHWDAPKL EAEITALLER HGLQYTLKWH RSGEPFYTPE GTLRAIARAV LAEHIGRAPE ESTGGGTSDA RFIAPLGAQC IEVGPVNASI HQVDENVRVD DLEALPGLYQ RLVERLLV
NT Sequence
TTGGCGGCGC GGCTGAAGCA GGCCGGATTC CAGTGCGATC ATCTGCGCCT GGGCGACGTT GACAATCTGT GGGCGACCCA TGGCCTGGGC GCGCCGGTGC TGGTTCTGCT GGGCCACACT GACGTGGTGC CCACCGGCCC GCGCGAGTCC TGGACCAGCG ACCCGTTCAC CCCACATATC CGCGATGGCG TGCTGTATGG GCGCGGTGCC GCCGACATGA AAGGCAGCGT GGCGGCGTTC GTGGTGGCCG CCGAGCAGTT CGTCGCCGAC CATCCCGACC ATCCCGGCAC GCTGGCGGTA CTGCTGACCA GCGACGAGGA GGGCGATGCC ATCGATGGCG TGCGCCACGT TGCACGCCTG TTCGCCGCAC GCGGGCAGCG CATCGACTGG TGCATCACCG GTGAGCCGTC GTCGACCGCG ACGCTGGGCG ATCTGCTGCG CGTGGGACGT CGCGGCAGCC TGTCGGCCAA ACTGCGCGTC CAAGGCGTGC AGGGCCACGT GGCGTATCCG GAAAAGGCAC GCAACCCGAT TCACCAGGCG GCGCCGGCCC TGGCAGAACT ATGCGCGCGG CGCTGGGACG ACGGCTACGA GAGTTTCCCG CCGACCAGCC TGCAGATCTC CAACATCCAC GCCGGCACCG GTGCCAACAA CGTGATTCCC GGTGAGCTCG ACGTGGATTT CAACATCCGC TACAACCCGC ACTGGGATGC GCCGAAGCTG GAGGCGGAGA TCACCGCACT GCTGGAACGG CATGGCCTGC AGTACACGCT GAAGTGGCAT CGCAGCGGCG AGCCGTTCTA CACCCCGGAA GGCACGCTGC GCGCCATCGC CCGTGCGGTG CTGGCCGAGC ACATCGGCCG CGCGCCAGAA GAAAGCACCG GCGGCGGCAC CTCCGATGCA CGCTTCATCG CGCCATTGGG CGCGCAATGC ATCGAAGTAG GCCCGGTCAA TGCCAGCATC CACCAGGTGG ACGAGAATGT GCGCGTGGAC GATCTGGAAG CGTTGCCGGG GCTGTACCAG CGGCTGGTGG AACGGCTGCT GGTG
Details for StmaA.17994.a.B1.GE43520
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML AARLKQAGFQ CDHLRLGDVD NLWATHGLGA PVLVLLGHTD VVPTGPRESW TSDPFTPHIR DGVLYGRGAA DMKGSVAAFV VAAEQFVADH PDHPGTLAVL LTSDEEGDAI DGVRHVARLF AARGQRIDWC ITGEPSSTAT LGDLLRVGRR GSLSAKLRVQ GVQGHVAYPE KARNPIHQAA PALAELCARR WDDGYESFPP TSLQISNIHA GTGANNVIPG ELDVDFNIRY NPHWDAPKLE AEITALLERH GLQYTLKWHR SGEPFYTPEG TLRAIARAVL AEHIGRAPEE STGGGTSDAR FIAPLGAQCI EVGPVNASIH QVDENVRVDD LEALPGLYQR
Validated NT Sequence
agccgctggt acagccccgg caacgcttcc agatcgtcca cgcgcacatt ctcgtccacc tggtggatgc tggcattgac cgggcctact tcgatgcatt gcgcgcccaa tggcgcgatg aagcgtgcat cggaggtgcc gccgccggtg ctttcttctg gcgcgcggcc gatgtgctcg gccagcaccg cacgggcgat ggcgcgcagc gtgccttccg gggtgtagaa cggctcgccg ctgcgatgcc acttcagcgt gtactgcagg ccatgccgtt ccagcagtgc ggtgatctcc gcctccagct tcggcgcatc ccagtgcggg ttgtagcgga tgttgaaatc cacgtcgagc tcaccgggaa tcacgttgtt ggcaccggtg ccggcgtgga tgttggagat ctgcaggctg gtcggcggga aactctcgta gccgtcgtcc cagcgccgcg cgcatagttc tgccagggcc ggcgccgcct ggtgaatcgg gttgcgtgcc ttttccggat acgccacgtg gccctgcacg ccttggacgc gcagtttggc cgacaggctg ccgcgacgtc ccacgcgcag cagatcgccc agcgtcgcgg tcgacgacgg ctcaccggtg atgcaccagt cgatgcgctg cccgcgtgcg gcgaacaggc gtgcaacgtg gcgcacgcca tcgatggcat cgccctcctc gtcgctggtc agcagtaccg ccagcgtgcc gggatggtcg ggatggtcgg cgacgaactg ctcggcggcc accacgaacg ccgccacgct gcctttcatg tcggcggcac cgcgcccata cagcacgcca tcgcggatat gtggggtgaa cgggtcgctg gtccaggact cgcgcgggcc ggtgggcacc acgtcagtgt ggcccagcag aaccagcacc ggcgcgccca ggccatgggt cgcccacaga ttgtcaacgt cgcccaggcg cagatgatcg cactggaatc cggcctgctt cagccgcgcc gccaacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMA ARLKQAGFQC DHLRLGDVDN LWATHGLGAP VLVLLGHTDV VPTGPRESWT SDPFTPHIRD GVLYGRGAAD MKGSVAAFVV AAEQFVADHP DHPGTLAVLL TSDEEGDAID GVRHVARLFA ARGQRIDWCI TGEPSSTATL GDLLRVGRRG SLSAKLRVQG VQGHVAYPEK ARNPIHQAAP ALAELCARRW DDGYESFPPT SLQISNIHAG TGANNVIPGE LDVDFNIRYN PHWDAPKLEA EITALLERHG LQYTLKWHRS GEPFYTPEGT LRAIARAVLA EHIGRAPEES TGGGTSDARF IAPLGAQCIE VGPVNASIHQ VDENVRVDDL EALPGLYQRL VERLLV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggcgg cgcggctgaa gcaggccgga ttccagtgcg atcatctgcg cctgggcgac gttgacaatc tgtgggcgac ccatggcctg ggcgcgccgg tgctggttct gctgggccac actgacgtgg tgcccaccgg cccgcgcgag tcctggacca gcgacccgtt caccccacat atccgcgatg gcgtgctgta tgggcgcggt gccgccgaca tgaaaggcag cgtggcggcg ttcgtggtgg ccgccgagca gttcgtcgcc gaccatcccg accatcccgg cacgctggcg gtactgctga ccagcgacga ggagggcgat gccatcgatg gcgtgcgcca cgttgcacgc ctgttcgccg cacgcgggca gcgcatcgac tggtgcatca ccggtgagcc gtcgtcgacc gcgacgctgg gcgatctgct gcgcgtggga cgtcgcggca gcctgtcggc caaactgcgc gtccaaggcg tgcagggcca cgtggcgtat ccggaaaagg cacgcaaccc gattcaccag gcggcgccgg ccctggcaga actatgcgcg cggcgctggg acgacggcta cgagagtttc ccgccgacca gcctgcagat ctccaacatc cacgccggca ccggtgccaa caacgtgatt cccggtgagc tcgacgtgga tttcaacatc cgctacaacc cgcactggga tgcgccgaag ctggaggcgg agatcaccgc actgctggaa cggcatggcc tgcagtacac gctgaagtgg catcgcagcg gcgagccgtt ctacaccccg gaaggcacgc tgcgcgccat cgcccgtgcg gtgctggccg agcacatcgg ccgcgcgcca gaagaaagca ccggcggcgg cacctccgat gcacgcttca tcgcgccatt gggcgcgcaa tgcatcgaag taggcccggt caatgccagc atccaccagg tggacgagaa tgtgcgcgtg gacgatctgg aagcgttgcc ggggctgtac cagcggctgg tggaacggct gctggtgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at