StmaA.17993.a

Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14) (glyQ)

CENTER ID: StmaA.17993.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17993.a.B1.GE43519 Full length(StmaA.17993.a) 1 303
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.4369

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSATPTVPIT FQGLIQTLNQ FWAQQGCVLI QPLDLEVGAG TFHPATFLRA IGPEGWNAAY VQPSRRPTDG RYGENPNRLQ RYYQYQVAMK PAPDNIQQLY LDSLKALGID PLVHDLRFVE DNWESPTLGA WGLGWEVWLN GMEVTQFTYF QQAGGLECRP VLGEITYGLE RLCMYLQNCD NVYDLIWTYG PDGQPVTYGD VYHQNEVEQS TYNFEYADVE EMFHRFDACE REAQKLVEVN LPLPAYEQVM KASHTFNLLD ARRAISVTER QRYILRVRAL AQAVARAYYE QREKLGFPGA KKA
NT Sequence
ATGTCCGCGA CCCCGACCGT TCCGATCACC TTCCAGGGCC TGATCCAGAC CCTGAACCAG TTCTGGGCCC AGCAGGGCTG CGTGCTCATC CAGCCGCTCG ACCTGGAGGT GGGCGCCGGT ACCTTCCACC CGGCCACCTT CCTGCGTGCG ATCGGTCCGG AAGGCTGGAA TGCGGCCTAC GTGCAGCCGT CGCGGCGCCC GACCGATGGC CGTTACGGTG AGAACCCGAA CCGCCTGCAG CGCTACTACC AGTACCAGGT GGCGATGAAG CCGGCGCCGG ACAACATCCA GCAGTTGTAC CTGGATTCGC TGAAGGCACT GGGCATCGAC CCGCTAGTGC ACGACCTGCG CTTCGTCGAG GACAACTGGG AATCGCCGAC GCTGGGCGCG TGGGGCCTGG GCTGGGAAGT CTGGCTCAAT GGCATGGAAG TGACCCAGTT CACCTACTTC CAGCAGGCCG GTGGCCTGGA ATGCCGCCCG GTGCTGGGCG AGATCACCTA CGGTCTCGAG CGCCTGTGCA TGTACCTGCA GAACTGCGAC AACGTCTACG ACCTGATCTG GACCTACGGC CCGGACGGCC AGCCGGTGAC CTACGGTGAC GTCTACCACC AGAACGAGGT GGAGCAGAGC ACCTACAACT TCGAATACGC CGACGTGGAA GAAATGTTCC ACCGTTTCGA CGCCTGCGAG CGTGAGGCGC AGAAGCTGGT GGAAGTGAAC CTGCCGCTGC CGGCCTACGA GCAGGTGATG AAGGCCAGCC ACACCTTCAA CCTGCTGGAT GCGCGCCGTG CGATCAGCGT GACCGAGCGC CAGCGCTACA TCCTGCGCGT GCGCGCGCTG GCCCAGGCGG TGGCCAGGGC GTACTACGAG CAGCGCGAGA AGCTGGGCTT CCCGGGCGCG AAGAAGGCC
Details for StmaA.17993.a.B1.GE43519
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS ATPTVPITFQ GLIQTLNQFW AQQGCVLIQP LDLEVGAGTF HPATFLRAIG PEGWNAAYVQ PSRRPTDGRY GENPNRLQRY YQYQVAMKPA PDNIQQLYLD SLKALGIDPL VHDLRFVEDN WESPTLGAWG LGWEVWLNGM EVTQFTYFQQ AGGLECRPVL GEITYGLERL CMYLQNCDNV YDLIWTYGPD GQPVTYGDVY HQNEVEQSTY NFEYADVEEM FHRFDACERE AQKLVEVNLP LPAYEQVMKA SHTFNLLDAR RAISVTERQR YILRVRALAQ AVARAYYEQR EKLGFPGAKK A
Validated NT Sequence
tactcnctta ggccttcttc gcgcccggga agcccagctt ctcgcgctgc tcgtagtacg ccctggccac cgcctgggcc agcgcgcgca cgcgcaggat gtagcgctgg cgctcggtca cgctgatcgc acggcgcgca tccagcaggt tgaaggtgtg gctggccttc atcacctgct cgtaggccgg cagcggcagg ttcacttcca ccagcttctg cgcctcacgc tcgcaggcgt cgaaacggtg gaacatttct tccacgtcgg cgtattcgaa gttgtaggtg ctctgctcca cctcgttctg gtggtagacg tcaccgtagg tcaccggctg gccgtccggg ccgtaggtcc agatcaggtc gtagacgttg tcgcagttct gcaggtacat gcacaggcgc tcgagaccgt aggtgatctc gcccagcacc gggcggcatt ccaggccacc ggcctgctgg aagtaggtga actgggtcac ttccatgcca ttgagccaga cttcccagcc caggccccac gcgcccagcg tcggcgattc ccagttgtcc tcgacgaagc gcaggtcgtg cactagcggg tcgatgccca gtgccttcag cgaatccagg tacaactgct ggatgttgtc cggcgccggc ttcatcgcca cctggtactg gtagtagcgc tgcaggcggt tcgggttctc accgtaacgg ccatcggtcg ggcgccgcga cggctgcacg taggccgcat tccagccttc cggaccgatc gcacgcagga aggtggccgg gtggaaggta ccggcgccca cctccaggtc gagcggctgg atgagcacgc agccctgctg ggcccagaac tggttcaggg tctggatcag gccctggaag gtgatcggaa cggtcggggt cgcggacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS ATPTVPITFQ GLIQTLNQFW AQQGCVLIQP LDLEVGAGTF HPATFLRAIG PEGWNAAYVQ PSRRPTDGRY GENPNRLQRY YQYQVAMKPA PDNIQQLYLD SLKALGIDPL VHDLRFVEDN WESPTLGAWG LGWEVWLNGM EVTQFTYFQQ AGGLECRPVL GEITYGLERL CMYLQNCDNV YDLIWTYGPD GQPVTYGDVY HQNEVEQSTY NFEYADVEEM FHRFDACERE AQKLVEVNLP LPAYEQVMKA SHTFNLLDAR RAISVTERQR YILRVRALAQ AVARAYYEQR EKLGFPGAKK A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg cgaccccgac cgttccgatc accttccagg gcctgatcca gaccctgaac cagttctggg cccagcaggg ctgcgtgctc atccagccgc tcgacctgga ggtgggcgcc ggtaccttcc acccggccac cttcctgcgt gcgatcggtc cggaaggctg gaatgcggcc tacgtgcagc cgtcgcggcg cccgaccgat ggccgttacg gtgagaaccc gaaccgcctg cagcgctact accagtacca ggtggcgatg aagccggcgc cggacaacat ccagcagttg tacctggatt cgctgaaggc actgggcatc gacccgctag tgcacgacct gcgcttcgtc gaggacaact gggaatcgcc gacgctgggc gcgtggggcc tgggctggga agtctggctc aatggcatgg aagtgaccca gttcacctac ttccagcagg ccggtggcct ggaatgccgc ccggtgctgg gcgagatcac ctacggtctc gagcgcctgt gcatgtacct gcagaactgc gacaacgtct acgacctgat ctggacctac ggcccggacg gccagccggt gacctacggt gacgtctacc accagaacga ggtggagcag agcacctaca acttcgaata cgccgacgtg gaagaaatgt tccaccgttt cgacgcctgc gagcgtgagg cgcagaagct ggtggaagtg aacctgccgc tgccggccta cgagcaggtg atgaaggcca gccacacctt caacctgctg gatgcgcgcc gtgcgatcag cgtgaccgag cgccagcgct acatcctgcg cgtgcgcgcg ctggcccagg cggtggccag ggcgtactac gagcagcgcg agaagctggg cttcccgggc gcgaagaagg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat