StmaA.17987.a
DNA polymerase III beta subunit (EC 2.7.7.7) (dnaN)
| CENTER ID: | StmaA.17987.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.17987.a.B1.GE43518 | Full length(StmaA.17987.a) | 1 | 366 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.17987.a.B1.PS38645 | Full length(StmaA.17987.a) | 1 | 366 |
Structures
7RZM
DEPOSITED: 8/27/2021
DETERMINATION: XRay
CLONE: StmaA.17987.a.B1.GE43518
PROTEIN: StmaA.17987.a.B1.PS38645
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFTLQREAF LKPLAQVVNV VERRQTLPVL ANFLVQVQNG QLSLTGTDLE VEMVSRIAVE DAQDGETTIP ARKLFEIIRA LPDGSRITVS QTGDKITVQA GRSRFTLATL PSNDFPSVDE VEATERVAIG EATLKELIER TAFAMAQQDV RYYLNGLLFD LRGDALRTVA TDGHRLALCE TDLAKPSGSK RQIIVPRKGV TELQRLLESG DREIELEVGR SHVRVKRDDV TFTSKLIDGR FPDYEAVIPI GADREVKVDR EALRASLQRA AILSNEKYRG IRVEVSPGNL KISAHNPEQE EAQEEIEADT TVSDLAIGFN VNYLLDALSA LRDEEVIIQL RDSNSSALVR ESSSEKSRHV VMPLRL
NT Sequence
ATGCGTTTCA CACTGCAGCG CGAAGCCTTT CTCAAGCCGT TGGCACAGGT CGTCAACGTG GTCGAACGCC GCCAGACCCT TCCGGTTCTG GCCAATTTCC TGGTCCAGGT GCAGAACGGC CAGCTGTCGC TGACCGGTAC CGACCTGGAA GTGGAGATGG TGTCGCGGAT CGCGGTTGAA GATGCCCAGG ACGGCGAAAC CACCATTCCC GCCCGCAAGC TGTTCGAGAT CATCCGCGCC CTGCCCGACG GCAGCCGGAT TACCGTCTCG CAGACCGGTG ACAAGATCAC CGTGCAGGCC GGCCGCAGCC GCTTCACCCT GGCCACCCTG CCCTCCAACG ACTTCCCGTC GGTGGACGAA GTGGAAGCCA CCGAGCGCGT GGCCATCGGC GAAGCAACCC TGAAGGAACT GATCGAGCGC ACTGCGTTCG CGATGGCCCA GCAGGACGTG CGCTACTACC TCAATGGCCT GCTGTTCGAC CTGCGCGGCG ATGCGCTGCG CACGGTCGCC ACCGACGGCC ATCGCCTGGC CCTGTGTGAA ACCGATCTGG CCAAGCCCAG CGGTTCCAAG CGCCAGATCA TCGTGCCGCG CAAGGGCGTG ACCGAACTGC AGCGCCTGCT GGAGAGCGGC GATCGCGAGA TCGAACTGGA AGTCGGCCGC AGCCACGTGC GCGTCAAGCG CGACGATGTC ACCTTCACCT CGAAGCTGAT CGACGGTCGT TTCCCGGATT ACGAAGCGGT GATTCCGATC GGTGCCGACC GCGAAGTGAA GGTTGATCGT GAAGCACTGC GTGCCTCGCT GCAGCGCGCC GCGATCCTGT CCAACGAGAA GTACCGCGGC ATCCGCGTGG AAGTCTCGCC GGGCAACCTG AAGATCAGCG CGCACAACCC GGAACAGGAA GAAGCCCAGG AAGAGATCGA AGCCGACACC ACGGTCAGTG ATCTGGCCAT TGGCTTCAAC GTGAACTACC TGCTGGATGC CCTCTCCGCG CTGCGCGATG AGGAGGTCAT CATCCAGCTG CGCGATTCCA ACTCTTCTGC GCTGGTTCGT GAATCCAGCA GTGAGAAGTC GCGTCACGTG GTCATGCCGC TGCGTCTC
Details for StmaA.17987.a.B1.GE43518
| HARVESTED ON: | 11/19/2019 |
| SEQUENCED ON: | 12/3/2019 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMR FTLQREAFLK PLAQVVNVVE RRQTLPVLAN FLVQVQNGQL SLTGTDLEVE MVSRIAVEDA QDGETTIPAR KLFEIIRALP DGSRITVSQT GDKITVQAGR SRFTLATLPS NDFPSVDEVE ATERVAIGEA TLKELIERTA FAMAQQDVRY YLNGLLFDLR GDALRTVATD GHRLALCETD LAKPSGSKRQ IIVPRKGVTE LQRLLESGDR EIELEVGRSH VRVKRDDVTF TSKLIDGRFP DYEAVIPIGA DREVKVDREA LRASLQRAAI LSNEKYRGIR VEVSPGNLKI SAHNPEQEEA QEEIEADTTV SDLAIGFNVN YLLDALSALR DEEVIIQLRD SNSSALVRES SSEKSR
Validated NT Sequence
gacgcgactt ctcactgctg gattcacgaa ccagcgcaga agagttggaa tcgcgcagct ggatgatgac ctcctcatcg cgcagcgcgg agagggcatc cagcaggtag ttcacgttga agccaatggc cagatcactg accgtggtgt cggcttcgat ctcttcctgg gcttcttcct gttccgggtt gtgcgcgctg atcttcaggt tgcccggcga gacttccacg cggatgccgc ggtacttctc gttggacagg atcgcggcgc gctgcagcga ggcacgcagt gcttcacgat caaccttcac ttcgcggtcg gcaccgatcg gaatcaccgc ttcgtaatcc gggaaacgac cgtcgatcag cttcgaggtg aaggtgacat cgtcgcgctt gacgcgcacg tggctgcggc cgacttccag ttcgatctcg cgatcgccgc tctccagcag gcgctgcagt tcggtcacgc ccttgcgcgg cacgatgatc tggcgcttgg aaccgctggg cttggccaga tcggtttcac acagggccag gcgatggccg tcggtggcga ccgtgcgcag cgcatcgccg cgcaggtcga acagcaggcc attgaggtag tagcgcacgt cctgctgggc catcgcgaac gcagtgcgct cgatcagttc cttcagggtt gcttcgccga tggccacgcg ctcggtggct tccacttcgt ccaccgacgg gaagtcgttg gagggcaggg tggccagggt gaagcggctg cggccggcct gcacggtgat cttgtcaccg gtctgcgaga cggtaatccg gctgccgtcg ggcagggcgc ggatgatctc gaacagcttg cgggcgggaa tggtggtttc gccgtcctgg gcatcttcaa ccgcgatccg cgacaccatc tccacttcca ggtcggtacc ggtcagcgac agctggccgt tctgcacctg gaccaggaaa ttggccagaa ccggaagggt ctggcggcgt tcgaccacgt tgacgacctg tgccaacggc ttgagaaagg cttcgcgctg cagtgtgaaa cgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMR FTLQREAFLK PLAQVVNVVE RRQTLPVLAN FLVQVQNGQL SLTGTDLEVE MVSRIAVEDA QDGETTIPAR KLFEIIRALP DGSRITVSQT GDKITVQAGR SRFTLATLPS NDFPSVDEVE ATERVAIGEA TLKELIERTA FAMAQQDVRY YLNGLLFDLR GDALRTVATD GHRLALCETD LAKPSGSKRQ IIVPRKGVTE LQRLLESGDR EIELEVGRSH VRVKRDDVTF TSKLIDGRFP DYEAVIPIGA DREVKVDREA LRASLQRAAI LSNEKYRGIR VEVSPGNLKI SAHNPEQEEA QEEIEADTTV SDLAIGFNVN YLLDALSALR DEEVIIQLRD SNSSALVRES SSEKSRHVVM PLRL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtt tcacactgca gcgcgaagcc tttctcaagc cgttggcaca ggtcgtcaac gtggtcgaac gccgccagac ccttccggtt ctggccaatt tcctggtcca ggtgcagaac ggccagctgt cgctgaccgg taccgacctg gaagtggaga tggtgtcgcg gatcgcggtt gaagatgccc aggacggcga aaccaccatt cccgcccgca agctgttcga gatcatccgc gccctgcccg acggcagccg gattaccgtc tcgcagaccg gtgacaagat caccgtgcag gccggccgca gccgcttcac cctggccacc ctgccctcca acgacttccc gtcggtggac gaagtggaag ccaccgagcg cgtggccatc ggcgaagcaa ccctgaagga actgatcgag cgcactgcgt tcgcgatggc ccagcaggac gtgcgctact acctcaatgg cctgctgttc gacctgcgcg gcgatgcgct gcgcacggtc gccaccgacg gccatcgcct ggccctgtgt gaaaccgatc tggccaagcc cagcggttcc aagcgccaga tcatcgtgcc gcgcaagggc gtgaccgaac tgcagcgcct gctggagagc ggcgatcgcg agatcgaact ggaagtcggc cgcagccacg tgcgcgtcaa gcgcgacgat gtcaccttca cctcgaagct gatcgacggt cgtttcccgg attacgaagc ggtgattccg atcggtgccg accgcgaagt gaaggttgat cgtgaagcac tgcgtgcctc gctgcagcgc gccgcgatcc tgtccaacga gaagtaccgc ggcatccgcg tggaagtctc gccgggcaac ctgaagatca gcgcgcacaa cccggaacag gaagaagccc aggaagagat cgaagccgac accacggtca gtgatctggc cattggcttc aacgtgaact acctgctgga tgccctctcc gcgctgcgcg atgaggaggt catcatccag ctgcgcgatt ccaactcttc tgcgctggtt cgtgaatcca gcagtgagaa gtcgcgtcac gtggtcatgc cgctgcgtct ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for StmaA.17987.a.B1.PS38645
| PURIFICATION DATe: | 9/22/2020 |
| CONCENTRATION: | 16.74mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR FTLQREAFLK PLAQVVNVVE RRQTLPVLAN FLVQVQNGQL SLTGTDLEVE MVSRIAVEDA QDGETTIPAR KLFEIIRALP DGSRITVSQT GDKITVQAGR SRFTLATLPS NDFPSVDEVE ATERVAIGEA TLKELIERTA FAMAQQDVRY YLNGLLFDLR GDALRTVATD GHRLALCETD LAKPSGSKRQ IIVPRKGVTE LQRLLESGDR EIELEVGRSH VRVKRDDVTF TSKLIDGRFP DYEAVIPIGA DREVKVDREA LRASLQRAAI LSNEKYRGIR VEVSPGNLKI SAHNPEQEEA QEEIEADTTV SDLAIGFNVN YLLDALSALR DEEVIIQLRD SNSSALVRES SSEKSR
Validated NT Sequence
gacgcgactt ctcactgctg gattcacgaa ccagcgcaga agagttggaa tcgcgcagct ggatgatgac ctcctcatcg cgcagcgcgg agagggcatc cagcaggtag ttcacgttga agccaatggc cagatcactg accgtggtgt cggcttcgat ctcttcctgg gcttcttcct gttccgggtt gtgcgcgctg atcttcaggt tgcccggcga gacttccacg cggatgccgc ggtacttctc gttggacagg atcgcggcgc gctgcagcga ggcacgcagt gcttcacgat caaccttcac ttcgcggtcg gcaccgatcg gaatcaccgc ttcgtaatcc gggaaacgac cgtcgatcag cttcgaggtg aaggtgacat cgtcgcgctt gacgcgcacg tggctgcggc cgacttccag ttcgatctcg cgatcgccgc tctccagcag gcgctgcagt tcggtcacgc ccttgcgcgg cacgatgatc tggcgcttgg aaccgctggg cttggccaga tcggtttcac acagggccag gcgatggccg tcggtggcga ccgtgcgcag cgcatcgccg cgcaggtcga acagcaggcc attgaggtag tagcgcacgt cctgctgggc catcgcgaac gcagtgcgct cgatcagttc cttcagggtt gcttcgccga tggccacgcg ctcggtggct tccacttcgt ccaccgacgg gaagtcgttg gagggcaggg tggccagggt gaagcggctg cggccggcct gcacggtgat cttgtcaccg gtctgcgaga cggtaatccg gctgccgtcg ggcagggcgc ggatgatctc gaacagcttg cgggcgggaa tggtggtttc gccgtcctgg gcatcttcaa ccgcgatccg cgacaccatc tccacttcca ggtcggtacc ggtcagcgac agctggccgt tctgcacctg gaccaggaaa ttggccagaa ccggaagggt ctggcggcgt tcgaccacgt tgacgacctg tgccaacggc ttgagaaagg cttcgcgctg cagtgtgaaa cgcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMR FTLQREAFLK PLAQVVNVVE RRQTLPVLAN FLVQVQNGQL SLTGTDLEVE MVSRIAVEDA QDGETTIPAR KLFEIIRALP DGSRITVSQT GDKITVQAGR SRFTLATLPS NDFPSVDEVE ATERVAIGEA TLKELIERTA FAMAQQDVRY YLNGLLFDLR GDALRTVATD GHRLALCETD LAKPSGSKRQ IIVPRKGVTE LQRLLESGDR EIELEVGRSH VRVKRDDVTF TSKLIDGRFP DYEAVIPIGA DREVKVDREA LRASLQRAAI LSNEKYRGIR VEVSPGNLKI SAHNPEQEEA QEEIEADTTV SDLAIGFNVN YLLDALSALR DEEVIIQLRD SNSSALVRES SSEKSRHVVM PLRL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTT TCACACTGCA GCGCGAAGCC TTTCTCAAGC CGTTGGCACA GGTCGTCAAC GTGGTCGAAC GCCGCCAGAC CCTTCCGGTT CTGGCCAATT TCCTGGTCCA GGTGCAGAAC GGCCAGCTGT CGCTGACCGG TACCGACCTG GAAGTGGAGA TGGTGTCGCG GATCGCGGTT GAAGATGCCC AGGACGGCGA AACCACCATT CCCGCCCGCA AGCTGTTCGA GATCATCCGC GCCCTGCCCG ACGGCAGCCG GATTACCGTC TCGCAGACCG GTGACAAGAT CACCGTGCAG GCCGGCCGCA GCCGCTTCAC CCTGGCCACC CTGCCCTCCA ACGACTTCCC GTCGGTGGAC GAAGTGGAAG CCACCGAGCG CGTGGCCATC GGCGAAGCAA CCCTGAAGGA ACTGATCGAG CGCACTGCGT TCGCGATGGC CCAGCAGGAC GTGCGCTACT ACCTCAATGG CCTGCTGTTC GACCTGCGCG GCGATGCGCT GCGCACGGTC GCCACCGACG GCCATCGCCT GGCCCTGTGT GAAACCGATC TGGCCAAGCC CAGCGGTTCC AAGCGCCAGA TCATCGTGCC GCGCAAGGGC GTGACCGAAC TGCAGCGCCT GCTGGAGAGC GGCGATCGCG AGATCGAACT GGAAGTCGGC CGCAGCCACG TGCGCGTCAA GCGCGACGAT GTCACCTTCA CCTCGAAGCT GATCGACGGT CGTTTCCCGG ATTACGAAGC GGTGATTCCG ATCGGTGCCG ACCGCGAAGT GAAGGTTGAT CGTGAAGCAC TGCGTGCCTC GCTGCAGCGC GCCGCGATCC TGTCCAACGA GAAGTACCGC GGCATCCGCG TGGAAGTCTC GCCGGGCAAC CTGAAGATCA GCGCGCACAA CCCGGAACAG GAAGAAGCCC AGGAAGAGAT CGAAGCCGAC ACCACGGTCA GTGATCTGGC CATTGGCTTC AACGTGAACT ACCTGCTGGA TGCCCTCTCC GCGCTGCGCG ATGAGGAGGT CATCATCCAG CTGCGCGATT CCAACTCTTC TGCGCTGGTT CGTGAATCCA GCAGTGAGAA GTCGCGTCAC GTGGTCATGC CGCTGCGTCT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT