StmaA.17982.a

CTP synthase (EC 6.3.4.2) (pyrG)

CENTER ID: StmaA.17982.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17982.a.B2.GE43517 mature protein 23 551
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1636

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFVTGGVVS SLGKGIAAAS LAAILEARGL KVTMMKLDPY INVDPGTMSP FQHGEVYVTD DGAETDLDLG HYERFVRTRL SRKNSVTTGR IYENVIRKER RGDYLGATVQ VIPHITDEIR RCIDEATEGY DVALVEIGGT VGDIESLPFL EAIRQVRTER GPEKALFMHL TLVPYIGAAG ELKTKPTQHS VKELRSIGIQ PDVLLCRSEQ AVPDSERRKI AQFTNVSERA VISVPDVDVL YRIPSGLHAQ GLDEIVVNQL KLADKAGPVD LSMWEDAVDA TLHPLDEVTI AVVGKYVDHQ DAYKSVGEAL KHGGLRQRTK VNLKWLEAQD LEGTDMAALA DVDGILVPGG FGDRGFEGKV LTSKFAREQQ VPYFGICYGM QAAVVDYARN VVGLEGANST ENDRQSPNPV IGLITEWRTA TGDVEKRDDK SDLGGTMRLG LQEQRLKPGT LARELYGKDV VAERHRHRYE FNNRYRTQLE DAGLVIAGKS MDDTLVEVVE LPRDAHPWFL ACQAHPEFLS TPRDGHPLFI GFIRAARERK AGGKLLSEAR A
NT Sequence
TTGATTTTCG TAACCGGCGG CGTGGTGTCC TCGCTCGGCA AAGGCATTGC CGCTGCGTCA CTGGCCGCCA TTCTCGAAGC CCGTGGCCTG AAGGTCACGA TGATGAAGCT CGATCCGTAC ATCAACGTCG ACCCGGGCAC CATGAGCCCA TTCCAGCACG GTGAGGTCTA CGTCACCGAC GACGGCGCCG AGACCGACCT CGACCTGGGC CACTACGAGC GCTTCGTGCG CACCCGCCTG AGCCGCAAGA ACTCGGTCAC CACCGGCCGC ATCTACGAGA ACGTGATCCG CAAGGAGCGC CGTGGCGACT ACCTGGGCGC GACGGTGCAG GTCATTCCGC ACATCACCGA CGAGATCCGC CGCTGCATCG ATGAAGCCAC GGAAGGCTAC GACGTGGCGC TGGTCGAGAT CGGCGGTACT GTCGGCGACA TCGAGTCGCT GCCGTTCCTG GAAGCGATCC GCCAGGTGCG CACCGAGCGT GGCCCGGAGA AGGCGCTGTT CATGCACCTT ACCCTGGTGC CGTACATCGG CGCCGCCGGC GAGCTGAAGA CCAAGCCGAC CCAGCATTCG GTGAAGGAGC TGCGCTCGAT CGGCATCCAG CCGGACGTGC TGCTGTGCCG CTCCGAGCAG GCGGTGCCGG ATTCGGAGCG CCGCAAGATC GCCCAGTTCA CCAACGTCTC CGAGCGCGCG GTGATCAGCG TGCCGGACGT GGACGTGCTG TACCGCATTC CGTCGGGCCT GCACGCGCAG GGCCTGGACG AGATCGTGGT CAACCAGCTG AAGCTGGCCG ACAAGGCCGG CCCGGTCGAC CTGTCGATGT GGGAAGACGC GGTCGACGCG ACCCTGCATC CGCTGGACGA AGTGACCATC GCCGTGGTTG GCAAGTACGT CGATCACCAG GATGCCTACA AGTCGGTCGG CGAAGCCCTC AAGCACGGTG GCCTGCGCCA GCGCACCAAG GTCAACCTGA AGTGGCTGGA AGCACAGGAC CTGGAAGGCA CCGACATGGC CGCACTGGCC GATGTCGACG GCATCCTGGT GCCGGGTGGT TTCGGTGACC GTGGTTTCGA AGGCAAGGTG CTGACCTCGA AGTTCGCCCG CGAGCAGCAG GTGCCGTACT TCGGCATCTG CTACGGCATG CAGGCGGCCG TGGTCGACTA CGCGCGCAAC GTGGTCGGCC TGGAAGGTGC CAACAGCACC GAGAACGACC GCCAGTCGCC GAACCCGGTG ATCGGCCTGA TCACCGAATG GCGCACCGCG ACCGGTGACG TGGAAAAGCG CGACGACAAG AGCGACCTCG GCGGCACCAT GCGCCTGGGC CTGCAGGAAC AGCGCCTGAA GCCGGGCACG CTGGCCCGCG AGCTGTATGG CAAGGACGTC GTCGCCGAGC GTCACCGCCA CCGTTACGAG TTCAACAACC GTTACCGCAC CCAGCTGGAA GATGCCGGCC TGGTGATCGC CGGCAAGTCG ATGGACGACA CCCTGGTGGA AGTGGTCGAA CTGCCGCGCG ACGCGCACCC GTGGTTCCTG GCCTGCCAGG CGCACCCGGA ATTCCTGTCC ACGCCGCGTG ACGGCCATCC GCTGTTCATC GGTTTCATCC GCGCCGCACG CGAGCGCAAG GCCGGCGGCA AGCTGCTTTC CGAAGCGCGC GCC
Details for StmaA.17982.a.B2.GE43517
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA ILEARGLKVT MMKLDPYINV DPGTMSPFQH GEVYVTDDGA ETDLDLGHYE RFVRTRLSRK NSVTTGRIYE NVIRKERRGD YLGATVQVIP HITDEIRRCI DEATEGYDVA LVEIGGTVGD IESLPFLEAI RQVRTERGPE KALFMHLTLV PYIGAAGELK TKPTQHSVKE LRSIGIQPDV LLCRSEQXVP DSERRKIXQF TNVSERAVIS VPDVDVLYRI PXGLHAQGLD XIVVNQLKLX DKAXPVDLSM WEDAVDATLH PLDEVTIAVV GKYVDHQDAY KSVGEAXKHG GXRQRTKVNL KWLEAQDLEG TDMAALADVD GILVPGGFGD RGFEGKVLTS KFAREQQVPY FGICYGMQAA VVDYARNVVG LEGANSTEND RQSPNPVIGL ITEWRTATGD VEKRDDKSDL GGTMRLGLQE QRLKPGTLAR ELYGKDVVAE RHRHRYEFNN RYRTQLEDAG LVIAGKSMDD TLVEVVELPR DAHPWFLACQ AHPEFLSTPR DGHPLFIGFI RAARERKAGG
Validated NT Sequence
ttgccgccgg ccttgcgctc gcgtgcggcg cggatgaaac cgatgaacag cggatggccg tcacgcggcg tggacaggaa ttccgggtgc gcctggcagg ccaggaacca cgggtgcgcg tcgcgcggca gttcgaccac ttccaccagg gtgtcgtcca tcgacttgcc ggcgatcacc aggccggcat cttccagctg ggtgcggtaa cggttgttga actcgtaacg gtggcggtga cgctcggcga cgacgtcctt gccatacagc tcgcgggcca gcgtgcccgg cttcaggcgc tgttcctgca ggcccaggcg catggtgccg ccgaggtcgc tcttgtcgtc gcgcttttcc acgtcaccgg tcgcggtgcg ccattcggtg atcaggccga tcaccgggtt cggcgactgg cggtcgttct cggtgctgtt ggcaccttcc aggccgacca cgttgcgcgc gtagtcgacc acggccgcct gcatgccgta gcagatgccg aagtacggca cctgctgctc gcgggcgaac ttcgaggtca gcaccttgcc ttcgaaacca cggtcaccga aaccacccgg caccaggatg ccgtcgacat cggccagtgc ggccatgtcg gtgccttcca ggtcctgtgc ttccagccac ttcaggttga ccttggtgcg ctggcgcang ccaccgtgct tganggcttc gccgaccgac ttgtangcat cctggtgatc gacgtacttg ccaaccacgg cgatggtcac ttcgtccagc ggatgcaggg tcgcgtcgac cgcgtcttcc cacatcgaca ggtcgaccgg gcnngccttg tcngncagct tcagctggtt gaccacgatn tcgtccaggc cctgcgcgtg caggcccnac ggaatgcggt acagcacgtc cacgtccggc acgctgatca ccgcgcgctc ggagacgttg gtgaactggn cgatcttgcg gcgctccgaa tccggcaccn cctgctcgga gcggcacagc agcacgtccg gctggatgcc gatcgagcgc agctccttca ccgaatgctg ggtcggcttg gtcttcagct cgccggcggc gccgatgtac ggcaccaggg taaggtgcat gaacagcgcc ttctccgggc cacgctcggt gcgcacctgg cggatcgctt ccaggaacgg cagcgactcg atgtcgccga cagtaccgcc gatctcgacc agcgccacgt cgtagccttc cgtggcttca tcgatgcagc ggcggatctc gtcggtgatg tgcggaatga cctgcaccgt cgcgcccagg tagtcgccac ggcgctcctt gcggatcacg ttctcgtaga tgcggccggt ggtgaccgag ttcttgcggc tcaggcgggt gcgcacgaag cgctcgtagt ggcccaggtc gaggtcggtc tcggcgccgt cgtcggtgac gtagacctca ccgtgctgga atgggctcat ggtgcccggg tcgacgttga tgtacggatc gagcttcatc atcgtgacct tcaggccacg ggcttcgaga atggccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHAI LEARGLKVTM MKLDPYINVD PGTMSPFQHG EVYVTDDGAE TDLDLGHYER FVRTRLSRKN SVTTGRIYEN VIRKERRGDY LGATVQVIPH ITDEIRRCID EATEGYDVAL VEIGGTVGDI ESLPFLEAIR QVRTERGPEK ALFMHLTLVP YIGAAGELKT KPTQHSVKEL RSIGIQPDVL LCRSEQAVPD SERRKIAQFT NVSERAVISV PDVDVLYRIP SGLHAQGLDE IVVNQLKLAD KAGPVDLSMW EDAVDATLHP LDEVTIAVVG KYVDHQDAYK SVGEALKHGG LRQRTKVNLK WLEAQDLEGT DMAALADVDG ILVPGGFGDR GFEGKVLTSK FAREQQVPYF GICYGMQAAV VDYARNVVGL EGANSTENDR QSPNPVIGLI TEWRTATGDV EKRDDKSDLG GTMRLGLQEQ RLKPGTLARE LYGKDVVAER HRHRYEFNNR YRTQLEDAGL VIAGKSMDDT LVEVVELPRD AHPWFLACQA HPEFLSTPRD GHPLFIGFIR AARERKAGGK LLSEARA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccattc tcgaagcccg tggcctgaag gtcacgatga tgaagctcga tccgtacatc aacgtcgacc cgggcaccat gagcccattc cagcacggtg aggtctacgt caccgacgac ggcgccgaga ccgacctcga cctgggccac tacgagcgct tcgtgcgcac ccgcctgagc cgcaagaact cggtcaccac cggccgcatc tacgagaacg tgatccgcaa ggagcgccgt ggcgactacc tgggcgcgac ggtgcaggtc attccgcaca tcaccgacga gatccgccgc tgcatcgatg aagccacgga aggctacgac gtggcgctgg tcgagatcgg cggtactgtc ggcgacatcg agtcgctgcc gttcctggaa gcgatccgcc aggtgcgcac cgagcgtggc ccggagaagg cgctgttcat gcaccttacc ctggtgccgt acatcggcgc cgccggcgag ctgaagacca agccgaccca gcattcggtg aaggagctgc gctcgatcgg catccagccg gacgtgctgc tgtgccgctc cgagcaggcg gtgccggatt cggagcgccg caagatcgcc cagttcacca acgtctccga gcgcgcggtg atcagcgtgc cggacgtgga cgtgctgtac cgcattccgt cgggcctgca cgcgcagggc ctggacgaga tcgtggtcaa ccagctgaag ctggccgaca aggccggccc ggtcgacctg tcgatgtggg aagacgcggt cgacgcgacc ctgcatccgc tggacgaagt gaccatcgcc gtggttggca agtacgtcga tcaccaggat gcctacaagt cggtcggcga agccctcaag cacggtggcc tgcgccagcg caccaaggtc aacctgaagt ggctggaagc acaggacctg gaaggcaccg acatggccgc actggccgat gtcgacggca tcctggtgcc gggtggtttc ggtgaccgtg gtttcgaagg caaggtgctg acctcgaagt tcgcccgcga gcagcaggtg ccgtacttcg gcatctgcta cggcatgcag gcggccgtgg tcgactacgc gcgcaacgtg gtcggcctgg aaggtgccaa cagcaccgag aacgaccgcc agtcgccgaa cccggtgatc ggcctgatca ccgaatggcg caccgcgacc ggtgacgtgg aaaagcgcga cgacaagagc gacctcggcg gcaccatgcg cctgggcctg caggaacagc gcctgaagcc gggcacgctg gcccgcgagc tgtatggcaa ggacgtcgtc gccgagcgtc accgccaccg ttacgagttc aacaaccgtt accgcaccca gctggaagat gccggcctgg tgatcgccgg caagtcgatg gacgacaccc tggtggaagt ggtcgaactg ccgcgcgacg cgcacccgtg gttcctggcc tgccaggcgc acccggaatt cctgtccacg ccgcgtgacg gccatccgct gttcatcggt ttcatccgcg ccgcacgcga gcgcaaggcc ggcggcaagc tgctttccga agcgcgcgcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat