SSGCID: Target Page

StmaA.17955.a

DNA polymerase III delta subunit (EC 2.7.7.7) (holA)

CENTER ID:	StmaA.17955.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.17955.a.B1.GE43443	Full length(StmaA.17955.a)		1	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3267

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MELRPEQLAS QGADTPLAPV YLIAGPETLR VLEAADAIRA RARAAGIAER EVFDADGRDF DWNQLDASFN APSLFSARRL VEVRLPSGKP GKDGAEVISQ FCANPAPDVV LMITASEWSK AHQGKWAEAV NRIGVLSVAW AIKPHELPDW IERRLRGKGL RADPAAVQRL AERVEGNLLA AAQEIDKLAL LADGQPLDVE RMESLVADAA RYDVFRLLEA TFSGQPPAVL RMLAGLRGEG EAVAALMPMV IRELLAGAGL ARVQARGGNL AAEMKSRGIW ESRQAPYKRA LQRHPEGKRW ERFVAEAGLV DRIAKGRADG DAWLALERLL VAVAEPRAVR LLARA

NT Sequence

ATGGAACTGC GTCCGGAGCA GCTGGCCAGC CAGGGCGCTG ACACCCCGCT GGCGCCGGTC TACCTGATTG CCGGCCCGGA AACCCTGCGC GTGCTTGAGG CGGCCGACGC CATCCGTGCA CGCGCACGCG CCGCCGGCAT CGCCGAGCGG GAAGTGTTCG ACGCCGATGG GCGTGATTTC GACTGGAACC AGCTCGACGC CAGCTTCAAC GCGCCCAGCC TGTTCAGTGC CCGCCGCCTG GTCGAGGTGC GCCTGCCCAG CGGCAAGCCG GGCAAGGACG GGGCCGAGGT GATCAGCCAG TTCTGCGCCA ACCCGGCGCC GGACGTGGTG CTGATGATTA CCGCCAGCGA ATGGAGCAAG GCCCACCAGG GCAAGTGGGC CGAGGCAGTC AATCGCATCG GCGTGCTGTC GGTGGCGTGG GCGATCAAGC CGCACGAACT GCCGGACTGG ATCGAGCGCC GCCTGCGTGG CAAGGGCCTG CGTGCCGATC CGGCCGCCGT GCAGCGCCTG GCCGAGCGGG TCGAGGGCAA CCTGCTGGCC GCTGCGCAGG AAATCGACAA GCTGGCGTTG CTGGCCGACG GCCAGCCGCT GGACGTGGAA CGGATGGAAT CGCTGGTGGC CGACGCCGCC CGCTATGACG TTTTCCGGCT GCTGGAAGCC ACGTTCTCCG GCCAGCCGCC GGCGGTGCTG CGCATGCTGG CGGGCCTGCG CGGCGAAGGC GAGGCGGTGG CCGCACTGAT GCCAATGGTG ATCCGCGAGC TGTTGGCTGG TGCCGGCCTG GCCCGGGTAC AGGCGCGTGG CGGCAATCTG GCCGCCGAAA TGAAATCGCG CGGCATCTGG GAATCGCGGC AAGCGCCCTA CAAGCGTGCG CTGCAGCGGC ACCCCGAGGG CAAGCGCTGG GAGCGTTTCG TGGCCGAGGC CGGACTGGTC GACCGCATCG CCAAGGGGCG CGCCGACGGT GATGCCTGGC TGGCGCTGGA GCGCCTGCTG GTGGCCGTGG CCGAACCGCG TGCCGTGCGC CTGCTGGCGC GGGCC

Details for StmaA.17955.a.B1.GE43443

HARVESTED ON:	9/18/2019
SEQUENCED ON:	10/1/2019
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHME LRPEQLASQG ADTPLAPVYL IAGPETLRVL EAADAIRARA RAAGIAEREV FDADGRDFDW NQLDASFNAP SLFSARRLVE VRLPSGKPGK DGAEVISQFC ANPAPDVVLM ITASEWSKAH QGKWAEAVNR IGVLSVAWAI KPHELPDWIE RRLRGKGLRA DPAAVQRLAE RVEGNLLAAA QEIDKLALLA DGQPLDVERM ESLVADAARY DVFRLLEATF SGQPPAVLRM LAGLRGEGEA VAALMPMVIR ELLAGAGLAR VQARGGNLAA EMKSRGIWES RQAPYKRALQ RHPEGKRWER FVAEAGLVDR IAKGRADGDA WLALERLLVA VAX

Validated NT Sequence

gntcggccac ggccaccagc aggcgctcca gcgccagcca ggcatcaccg tcggcgcgcc ccttggcgat gcggtcgacc agtccggcct cggccacgaa acgctcccag cgcttgccct cggggtgccg ctgcagcgca cgcttgtagg gcgcttgccg cgattcccag atgccgcgcg atttcatttc ggcggccaga ttgccgccac gcgcctgtac ccgggccagg ccggcaccag ccaacagctc gcggatcacc attggcatca gtgcggccac cgcctcgcct tcgccgcgca ggcccgccag catgcgcagc accgccggcg gctggccgga gaacgtggct tccagcagcc ggaaaacgtc atagcgggcg gcgtcggcca ccagcgattc catccgttcc acgtccagcg gctggccgtc ggccagcaac gccagcttgt cgatttcctg cgcagcggcc agcaggttgc cctcgacccg ctcggccagg cgctgcacgg cggccggatc ggcacgcagg cccttgccac gcaggcggcg ctcgatccag tccggcagtt cgtgcggctt gatcgcccac gccaccgaca gcacgccgat gcgattgact gcctcggccc acttgccctg gtgggccttg ctccattcgc tggcggtaat catcagcacc acgtccggcg ccgggttggc gcagaactgg ctgatcacct cggccccgtc cttgcccggc ttgccgctgg gcaggcgcac ctcgaccagg cggcgggcac tgaacaggct gggcgcgttg aagctggcgt cgagctggtt ccagtcgaaa tcacgcccat cggcgtcgaa cacttcccgc tcggcgatgc cggcggcgcg tgcgcgtgca cggatggcgt cggccgcctc aagcacgcgc agggtttccg ggccggcaat caggtagacc ggcgccagcg gggtgtcagc gccctggctg gccagctgct ccggacgcag ttccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac tgcgtccgga gcagctggcc agccagggcg ctgacacccc gctggcgccg gtctacctga ttgccggccc ggaaaccctg cgcgtgcttg aggcggccga cgccatccgt gcacgcgcac gcgccgccgg catcgccgag cgggaagtgt tcgacgccga tgggcgtgat ttcgactgga accagctcga cgccagcttc aacgcgccca gcctgttcag tgcccgccgc ctggtcgagg tgcgcctgcc cagcggcaag ccgggcaagg acggggccga ggtgatcagc cagttctgcg ccaacccggc gccggacgtg gtgctgatga ttaccgccag cgaatggagc aaggcccacc agggcaagtg ggccgaggca gtcaatcgca tcggcgtgct gtcggtggcg tgggcgatca agccgcacga actgccggac tggatcgagc gccgcctgcg tggcaagggc ctgcgtgccg atccggccgc cgtgcagcgc ctggccgagc gggtcgaggg caacctgctg gccgctgcgc aggaaatcga caagctggcg ttgctggccg acggccagcc gctggacgtg gaacggatgg aatcgctggt ggccgacgcc gcccgctatg acgttttccg gctgctggaa gccacgttct ccggccagcc gccggcggtg ctgcgcatgc tggcgggcct gcgcggcgaa ggcgaggcgg tggccgcact gatgccaatg gtgatccgcg agctgttggc tggtgccggc ctggcccggg tacaggcgcg tggcggcaat ctggccgccg aaatgaaatc gcgcggcatc tgggaatcgc ggcaagcgcc ctacaagcgt gcgctgcagc ggcaccccga gggcaagcgc tgggagcgtt tcgtggccga ggccggactg gtcgaccgca tcgccaaggg gcgcgccgac ggtgatgcct ggctggcgct ggagcgcctg ctggtggccg tggccgaacc gcgtgccgtg cgcctgctgg cgcgggcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat