StmaA.17954.a

Lipid-A-disaccharide synthase (EC 2.4.1.182) (lpxB)

CENTER ID: StmaA.17954.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17954.a.B2.GE43442 mature protein 27 419
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1431

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTTGQAPA GAAVIPLASM AGSVPAQRVL SERPLRIALV AGEASGDLLG AGLVRELKAR FPNAEFAGIG GDAMRSAGCL TWHDASELAV MGLTEVLRHL PRLLKLRSAF RQRALEWQPD VFIGIDAPDF NLGIERWLKQ RGVRTVHYVS PSVWAWREKR AEKIGSSADL VLCLFPMEPP IYARHGIDAR FVGHPMADDI PLQGNREEAR AALGLPTSAK VLAVLPGSRL GEISRLGEPF FEAAWQVSER IPGLHVVVPA ANPACKRLIE EQLSRSALPV AFSHVLDGEA RNAMIAADVV VLASGTATLE AMLVKRPMVV GYRVNELTYR LVKALGLIKV DRFALPNILA GQDLAPELMQ HDCTPDKLAA AIQQWFDHPQ RVTDLQDTYA RLHQRLRRNA SARAADAVGE LLMRDQAQA
NT Sequence
ATGAGCAGCA CGACCGGCCA GGCGCCGGCC GGCGCCGCCG TGATTCCGCT GGCCTCGATG GCCGGCAGCG TCCCCGCGCA GCGGGTGCTC AGCGAGCGCC CGTTGCGGAT CGCTCTGGTG GCCGGTGAGG CCTCCGGTGA TCTGCTGGGT GCGGGCCTGG TGCGCGAGCT GAAGGCACGC TTTCCGAATG CCGAATTCGC CGGCATCGGT GGCGATGCCA TGCGCAGCGC CGGCTGCCTG ACCTGGCACG ATGCCAGCGA GCTGGCGGTG ATGGGCCTGA CCGAAGTGCT GCGCCACCTG CCACGCCTGC TGAAACTGCG TTCCGCGTTC CGCCAGCGTG CACTGGAATG GCAACCGGAC GTGTTCATCG GCATCGACGC GCCCGACTTC AACCTGGGCA TCGAACGCTG GCTGAAGCAG CGCGGCGTGC GCACCGTACA CTACGTCAGC CCCTCCGTCT GGGCCTGGCG CGAGAAGCGC GCCGAGAAGA TCGGCAGCAG CGCCGACCTG GTGCTGTGCC TGTTCCCGAT GGAACCCCCG ATCTATGCCA GGCACGGCAT CGACGCGCGC TTCGTTGGCC ATCCGATGGC CGATGACATC CCCCTGCAGG GCAACCGCGA GGAAGCGCGC GCCGCGCTTG GCCTGCCGAC CTCGGCCAAG GTGCTGGCGG TGCTGCCAGG CAGCCGCCTG GGCGAAATCT CCCGCCTCGG TGAACCGTTC TTCGAGGCCG CCTGGCAGGT CTCCGAGCGC ATCCCCGGCC TGCACGTGGT AGTGCCTGCC GCCAACCCGG CCTGCAAGCG CCTGATCGAA GAACAGCTGT CACGCTCGGC GCTGCCGGTA GCCTTCTCGC ATGTACTCGA CGGTGAGGCG CGAAACGCGA TGATCGCCGC CGATGTGGTG GTGCTGGCCT CGGGCACCGC GACGCTGGAG GCGATGCTGG TCAAGCGGCC GATGGTGGTC GGTTACCGCG TCAACGAGCT GACCTACCGC CTGGTCAAGG CGCTGGGCCT GATCAAGGTC GACCGCTTTG CCCTGCCCAA CATCCTGGCG GGCCAGGACC TGGCACCGGA ACTGATGCAG CACGATTGCA CGCCGGACAA GCTGGCGGCG GCCATCCAGC AATGGTTCGA CCACCCGCAG CGGGTGACCG ACCTGCAGGA CACCTATGCC CGCCTGCATC AACGACTGCG CCGCAATGCC TCGGCCCGCG CGGCCGACGC TGTGGGCGAG CTGTTGATGC GCGACCAGGC CCAGGCA
Details for StmaA.17954.a.B2.GE43442
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ RVLSERPLRI ALVAGEASGD LLGAGLVREL KARFPNAEFA GIGGDAMRSA GCLTWHDASE LAVMGLTEVL RHLPRLLKLR SAFRQRALEW QPDVFIGIDA PDFNLGIERW LKQRGVRTVH YVSPSVWAWR EKRAEKIGSS ADLVLCLFPM EPPIYARHGI DARFVGHPMA DDIPLQGNRE EARAALGLPT SAKVLAVLPG SRLGEISRLG EPFFEAAWQV SERIPGLHVV VPAANPACKR LIEEQLSRSA LPVAFSHVLD GEARNAMIAA DVVVLASGTA TLEAMLVKRP MVVGYRVNEL TYRLVKALGL IKVDRFALPN ILAGQDLAPE LMQHDCTPDK LAAAIQQWFD HPQRVTDLQD TYARLHQRLR RNASARAADA VGXX
Validated NT Sequence
cannnnntcg cccacagcgt cggccgcgcg ggccgaggca ttgcggcgca gtcgttgatg caggcgggca taggtgtcct gcaggtcggt cacccgctgc gggtggtcga accattgctg gatggccgcc gccagcttgt ccggcgtgca atcgtgctgc atcagttccg gtgccaggtc ctggcccgcc aggatgttgg gcagggcaaa gcggtcgacc ttgatcaggc ccagcgcctt gaccaggcgg taggtcagct cgttgacgcg gtaaccgacc accatcggcc gcttgaccag catcgcctcc agcgtcgcgg tgcccgaggc cagcaccacc acatcggcgg cgatcatcgc gtttcgcgcc tcaccgtcga gtacatgcga gaaggctacc ggcagcgccg agcgtgacag ctgttcttcg atcaggcgct tgcaggccgg gttggcggca ggcactacca cgtgcaggcc ggggatgcgc tcggagacct gccaggcggc ctcgaagaac ggttcaccga ggcgggagat ttcgcccagg cggctgcctg gcagcaccgc cagcaccttg gccgaggtcg gcaggccaag cgcggcgcgc gcttcctcgc ggttgccctg cagggggatg tcatcggcca tcggatggcc aacgaagcgc gcgtcgatgc cgtgcctggc atagatcggg ggttccatcg ggaacaggca cagcaccagg tcggcgctgc tgccgatctt ctcggcgcgc ttctcgcgcc aggcccagac ggaggggctg acgtagtgta cggtgcgcac gccgcgctgc ttcagccagc gttcgatgcc caggttgaag tcgggcgcgt cgatgccgat gaacacgtcc ggttgccatt ccagtgcacg ctggcggaac gcggaacgca gtttcagcag gcgtggcagg tggcgcagca cttcggtcag gcccatcacc gccagctcgc tggcatcgtg ccaggtcagg cagccggcgc tgcgcatggc atcgccaccg atgccggcga attcggcatt cggaaagcgt gccttcagct cgcgcaccag gcccgcaccc agcagatcac cggaggcctc accggccacc agagcgatcc gcaacgggcg ctcgctgagc acccgctgca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHQR VLSERPLRIA LVAGEASGDL LGAGLVRELK ARFPNAEFAG IGGDAMRSAG CLTWHDASEL AVMGLTEVLR HLPRLLKLRS AFRQRALEWQ PDVFIGIDAP DFNLGIERWL KQRGVRTVHY VSPSVWAWRE KRAEKIGSSA DLVLCLFPME PPIYARHGID ARFVGHPMAD DIPLQGNREE ARAALGLPTS AKVLAVLPGS RLGEISRLGE PFFEAAWQVS ERIPGLHVVV PAANPACKRL IEEQLSRSAL PVAFSHVLDG EARNAMIAAD VVVLASGTAT LEAMLVKRPM VVGYRVNELT YRLVKALGLI KVDRFALPNI LAGQDLAPEL MQHDCTPDKL AAAIQQWFDH PQRVTDLQDT YARLHQRLRR NASARAADAV GELLMRDQAQ A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagcggg tgctcagcga gcgcccgttg cggatcgctc tggtggccgg tgaggcctcc ggtgatctgc tgggtgcggg cctggtgcgc gagctgaagg cacgctttcc gaatgccgaa ttcgccggca tcggtggcga tgccatgcgc agcgccggct gcctgacctg gcacgatgcc agcgagctgg cggtgatggg cctgaccgaa gtgctgcgcc acctgccacg cctgctgaaa ctgcgttccg cgttccgcca gcgtgcactg gaatggcaac cggacgtgtt catcggcatc gacgcgcccg acttcaacct gggcatcgaa cgctggctga agcagcgcgg cgtgcgcacc gtacactacg tcagcccctc cgtctgggcc tggcgcgaga agcgcgccga gaagatcggc agcagcgccg acctggtgct gtgcctgttc ccgatggaac ccccgatcta tgccaggcac ggcatcgacg cgcgcttcgt tggccatccg atggccgatg acatccccct gcagggcaac cgcgaggaag cgcgcgccgc gcttggcctg ccgacctcgg ccaaggtgct ggcggtgctg ccaggcagcc gcctgggcga aatctcccgc ctcggtgaac cgttcttcga ggccgcctgg caggtctccg agcgcatccc cggcctgcac gtggtagtgc ctgccgccaa cccggcctgc aagcgcctga tcgaagaaca gctgtcacgc tcggcgctgc cggtagcctt ctcgcatgta ctcgacggtg aggcgcgaaa cgcgatgatc gccgccgatg tggtggtgct ggcctcgggc accgcgacgc tggaggcgat gctggtcaag cggccgatgg tggtcggtta ccgcgtcaac gagctgacct accgcctggt caaggcgctg ggcctgatca aggtcgaccg ctttgccctg cccaacatcc tggcgggcca ggacctggca ccggaactga tgcagcacga ttgcacgccg gacaagctgg cggcggccat ccagcaatgg ttcgaccacc cgcagcgggt gaccgacctg caggacacct atgcccgcct gcatcaacga ctgcgccgca atgcctcggc ccgcgcggcc gacgctgtgg gcgagctgtt gatgcgcgac caggcccagg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat