SSGCID: Target Page

StmaA.17953.a

Electron transfer flavoprotein, beta subunit (etfB)

CENTER ID:	StmaA.17953.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.17953.a.B1.GE43441	Full length(StmaA.17953.a)		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.615

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKILVAYKRV VDYNVRIQVK PDGSGVVTDG VKLSPNPFDE IALEEALRLR DKGIATEVVV ATIAPADAQA HLRNGLAMGA NRAIHVVTDQ AIQPLTASRT LLKLIEKEQP DLVILGKQAI DDDANQTGQM LATLWGRPQA TFASKLEIAD GKATVTREVD AGLETLEVDL PAVVTTDLRL NEPRFIKLPD IMKAKAKPLE TLQLADLGVE AADTFKTTQY VAPSKRSKGV MVKDAAELVA ALKQKGLL

NT Sequence

ATGAAAATCC TCGTCGCGTA CAAGCGCGTG GTGGACTACA ACGTCCGCAT TCAGGTCAAG CCGGACGGTT CCGGCGTGGT CACCGACGGC GTCAAGCTAT CCCCCAACCC CTTCGATGAA ATCGCGCTGG AAGAAGCCCT GCGCCTGCGC GACAAAGGCA TCGCCACGGA AGTGGTGGTC GCCACCATCG CCCCGGCCGA CGCCCAGGCG CACCTGCGCA ACGGCCTGGC CATGGGCGCC AACCGCGCCA TCCACGTGGT GACCGACCAG GCCATCCAGC CGCTGACCGC CTCGCGCACC CTGCTGAAGC TGATCGAGAA GGAACAGCCG GACCTGGTGA TCCTGGGCAA GCAGGCCATC GACGACGACG CCAACCAGAC CGGTCAGATG CTGGCCACGC TGTGGGGCCG TCCGCAGGCG ACCTTCGCCA GCAAGCTCGA GATCGCCGAC GGCAAGGCCA CGGTGACCCG CGAAGTCGAC GCCGGCCTGG AAACGCTGGA AGTGGACCTG CCGGCCGTGG TCACCACCGA CCTGCGCCTG AACGAGCCGC GCTTCATCAA GCTGCCGGAC ATCATGAAGG CCAAGGCCAA GCCGCTGGAA ACCCTGCAGC TGGCCGACCT CGGTGTTGAA GCCGCCGATA CCTTCAAGAC CACCCAGTAC GTCGCGCCGT CCAAGCGCAG CAAGGGTGTG ATGGTCAAGG ACGCGGCCGA ACTGGTGGCC GCACTCAAGC AGAAGGGGCT GCTG

Details for StmaA.17953.a.B1.GE43441

HARVESTED ON:	9/18/2019
SEQUENCED ON:	10/1/2019
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK ILVAYKRVVD YNVRIQVKPD GSGVVTDGVK LSPNPFDEIA LEEALRLRDK GIATEVVVAT IAPADAQAHL RNGLAMGANR AIHVVTDQAI QPLTASRTLL KLIEKEQPDL VILGKQAIDD DANQTGQMLA TLWGRPQATF ASKLEIADGK ATVTREVDAG LETLEVDLPA VVTTDLRLNE PRFIKLPDIM KAKAKPLETL QLADLGVEAA DTFKTTQYVA PSKRSKGVMV KDAAELVAAL KQKGLL

Validated NT Sequence

atcttactca cttacagcag ccccttctgc ttgagtgcgg ccaccagttc ggccgcgtcc ttgaccatca cacccttgct gcgcttggac ggcgcgacgt actgggtggt cttgaaggta tcggcggctt caacaccgag gtcggccagc tgcagggttt ccagcggctt ggccttggcc ttcatgatgt ccggcagctt gatgaagcgc ggctcgttca ggcgcaggtc ggtggtgacc acggccggca ggtccacttc cagcgtttcc aggccggcgt cgacttcgcg ggtcaccgtg gccttgccgt cggcgatctc gagcttgctg gcgaaggtcg cctgcggacg gccccacagc gtggccagca tctgaccggt ctggttggcg tcgtcgtcga tggcctgctt gcccaggatc accaggtccg gctgttcctt ctcgatcagc ttcagcaggg tgcgcgaggc ggtcagcggc tggatggcct ggtcggtcac cacgtggatg gcgcggttgg cgcccatggc caggccgttg cgcaggtgcg cctgggcgtc ggccggggcg atggtggcga ccaccacttc cgtggcgatg cctttgtcgc gcaggcgcag ggcttcttcc agcgcgattt catcgaaggg gttgggggat agcttgacgc cgtcggtgac cacgccggaa ccgtccggct tgacctgaat gcggacgttg tagtccacca cgcgcttgta cgcgacgagg attttcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tcctcgtcgc gtacaagcgc gtggtggact acaacgtccg cattcaggtc aagccggacg gttccggcgt ggtcaccgac ggcgtcaagc tatcccccaa ccccttcgat gaaatcgcgc tggaagaagc cctgcgcctg cgcgacaaag gcatcgccac ggaagtggtg gtcgccacca tcgccccggc cgacgcccag gcgcacctgc gcaacggcct ggccatgggc gccaaccgcg ccatccacgt ggtgaccgac caggccatcc agccgctgac cgcctcgcgc accctgctga agctgatcga gaaggaacag ccggacctgg tgatcctggg caagcaggcc atcgacgacg acgccaacca gaccggtcag atgctggcca cgctgtgggg ccgtccgcag gcgaccttcg ccagcaagct cgagatcgcc gacggcaagg ccacggtgac ccgcgaagtc gacgccggcc tggaaacgct ggaagtggac ctgccggccg tggtcaccac cgacctgcgc ctgaacgagc cgcgcttcat caagctgccg gacatcatga aggccaaggc caagccgctg gaaaccctgc agctggccga cctcggtgtt gaagccgccg ataccttcaa gaccacccag tacgtcgcgc cgtccaagcg cagcaagggt gtgatggtca aggacgcggc cgaactggtg gccgcactca agcagaaggg gctgctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at