StmaA.17936.a
Fructose-1,6-bisphosphatase, type I (EC 3.1.3.11)
| CENTER ID: | StmaA.17936.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.17936.a.B1.GE43440 | Full length(StmaA.17936.a) | 1 | 333 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.17936.a.B1.PW38784 | Full length(StmaA.17936.a) | 1 | 333 |
Structures
7K74
DEPOSITED: 9/22/2020
DETERMINATION: XRay
CLONE: StmaA.17936.a.B1.GE43440
PROTEIN: StmaA.17936.a.B1.PW38784
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.39 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRFLIQEQH AGRINADLRQ LIAVVARACT SISIAVSKGA LGGVLGDAGT GNVQGEAQKK LDVISNEILL EANAWGGHLA ACASEEMDHS QPVPDIYPRG DFLLLFDPLD GSSNIDVNVS VGTIFSVLRC PTNVELPGDD AFLQPGSKQI AAGYCIYGPS TQLVLTVGHG THAFTLDREK GEFVLTTENM QIPAATQEFA INMSNQRHWE APMQAYVGDL LAGKEGTRGK NFNMRWIASM VADVHRILTR GGIFIYPWDK KDPSKAGKLR LMYEANPMGL LVEQAGGAAW TGRERILDIQ PDQLHQRVPV FLGSREEVAE AVRYHHAHDN AQG
NT Sequence
TTGACCCGCT TCCTGATCCA GGAACAGCAC GCCGGCCGCA TCAACGCCGA CCTGCGCCAG CTGATCGCGG TCGTCGCCCG CGCCTGCACC AGCATCTCCA TCGCCGTCAG CAAGGGCGCC CTCGGTGGCG TGCTGGGCGA TGCCGGTACC GGTAACGTGC AGGGCGAGGC GCAGAAGAAG CTGGACGTCA TCAGCAACGA GATCCTGCTT GAAGCGAACG CCTGGGGTGG CCACCTCGCC GCGTGCGCCT CGGAGGAAAT GGACCACAGC CAGCCGGTGC CGGACATCTA CCCGCGCGGT GATTTCCTGC TGCTGTTCGA TCCCCTCGAC GGCAGCTCCA ACATCGACGT CAACGTCTCC GTCGGCACCA TCTTCTCGGT GCTGCGCTGC CCGACCAATG TCGAACTGCC GGGCGACGAC GCCTTCCTGC AGCCGGGCAG CAAGCAGATC GCCGCCGGCT ACTGCATCTA CGGGCCCAGC ACCCAGCTGG TGCTGACCGT CGGCCATGGC ACCCACGCCT TCACCCTGGA CCGTGAGAAG GGCGAGTTCG TGCTGACCAC CGAGAACATG CAGATTCCGG CGGCTACCCA GGAGTTCGCG ATCAACATGT CCAACCAGCG CCACTGGGAA GCACCGATGC AGGCCTATGT CGGCGATCTG CTGGCGGGCA AGGAAGGCAC GCGCGGCAAG AACTTCAACA TGCGCTGGAT CGCCAGCATG GTCGCCGACG TGCACCGCAT CCTGACCCGC GGCGGCATCT TCATCTACCC GTGGGACAAG AAGGATCCGT CCAAGGCCGG CAAGCTGCGC CTGATGTACG AGGCCAACCC GATGGGCCTG CTGGTCGAAC AGGCCGGCGG CGCCGCCTGG ACCGGCCGCG AGCGCATCCT CGACATCCAG CCCGACCAGC TGCACCAGCG CGTGCCGGTG TTCCTGGGTT CGCGCGAGGA AGTGGCCGAA GCCGTGCGCT ACCACCACGC ACACGACAAC GCACAGGGC
Details for StmaA.17936.a.B1.GE43440
| HARVESTED ON: | 9/18/2019 |
| SEQUENCED ON: | 10/1/2019 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHML TRFLIQEQHA GRINADLRQL IAVVARACTS ISIAVSKGAL GGVLGDAGTG NVQGEAQKKL DVISNEILLE ANAWGGHLAA CASEEMDHSQ PVPDIYPRGD FLLLFDPLDG SSNIDVNVSV GTIFSVLRCP TNVELPGDDA FLQPGSKQIA AGYCIYGPST QLVLTVGHGT HAFTLDREKG EFVLTTENMQ IPAATQEFAI NMSNQRHWEA PMQAYVGDLL AGKEGTRGKN FNMRWIASMV ADVHRILTRG GIFIYPWDKK DPSKAGKLRL MYEANPMGLL VEQAGGAAWT GRERILDIQP DQLHQRVPVF LGSREEVAEA V
Validated NT Sequence
gcgcacggct tcggccactt cctcgcgcga acccaggaac accggcacgc gctggtgcag ctggtcgggc tggatgtcga ggatgcgctc gcggccggtc caggcggcgc cgccggcctg ttcgaccagc aggcccatcg ggttggcctc gtacatcagg cgcagcttgc cggccttgga cggatccttc ttgtcccacg ggtagatgaa gatgccgccg cgggtcagga tgcggtgcac gtcggcgacc atgctggcga tccagcgcat gttgaagttc ttgccgcgcg tgccttcctt gcccgccagc agatcgccga cataggcctg catcggtgct tcccagtggc gctggttgga catgttgatc gcgaactcct gggtagccgc cggaatctgc atgttctcgg tggtcagcac gaactcgccc ttctcacggt ccagggtgaa ggcgtgggtg ccatggccga cggtcagcac cagctgggtg ctgggcccgt agatgcagta gccggcggcg atctgcttgc tgcccggctg caggaaggcg tcgtcgcccg gcagttcgac attggtcggg cagcgcagca ccgagaagat ggtgccgacg gagacgttga cgtcgatgtt ggagctgccg tcgaggggat cgaacagcag caggaaatca ccgcgcgggt agatgtccgg caccggctgg ctgtggtcca tttcctccga ggcgcacgcg gcgaggtggc caccccaggc gttcgcttca agcaggatct cgttgctgat gacgtccagc ttcttctgcg cctcgccctg cacgttaccg gtaccggcat cgcccagcac gccaccgagg gcgcccttgc tgacggcgat ggagatgctg gtgcaggcgc gggcgacgac cgcgatcagc tggcgcaggt cggcgttgat gcggccggcg tgctgttcct ggatcaggaa gcgggtcaac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMT RFLIQEQHAG RINADLRQLI AVVARACTSI SIAVSKGALG GVLGDAGTGN VQGEAQKKLD VISNEILLEA NAWGGHLAAC ASEEMDHSQP VPDIYPRGDF LLLFDPLDGS SNIDVNVSVG TIFSVLRCPT NVELPGDDAF LQPGSKQIAA GYCIYGPSTQ LVLTVGHGTH AFTLDREKGE FVLTTENMQI PAATQEFAIN MSNQRHWEAP MQAYVGDLLA GKEGTRGKNF NMRWIASMVA DVHRILTRGG IFIYPWDKKD PSKAGKLRLM YEANPMGLLV EQAGGAAWTG RERILDIQPD QLHQRVPVFL GSREEVAEAV RYHHAHDNAQ G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaccc gcttcctgat ccaggaacag cacgccggcc gcatcaacgc cgacctgcgc cagctgatcg cggtcgtcgc ccgcgcctgc accagcatct ccatcgccgt cagcaagggc gccctcggtg gcgtgctggg cgatgccggt accggtaacg tgcagggcga ggcgcagaag aagctggacg tcatcagcaa cgagatcctg cttgaagcga acgcctgggg tggccacctc gccgcgtgcg cctcggagga aatggaccac agccagccgg tgccggacat ctacccgcgc ggtgatttcc tgctgctgtt cgatcccctc gacggcagct ccaacatcga cgtcaacgtc tccgtcggca ccatcttctc ggtgctgcgc tgcccgacca atgtcgaact gccgggcgac gacgccttcc tgcagccggg cagcaagcag atcgccgccg gctactgcat ctacgggccc agcacccagc tggtgctgac cgtcggccat ggcacccacg ccttcaccct ggaccgtgag aagggcgagt tcgtgctgac caccgagaac atgcagattc cggcggctac ccaggagttc gcgatcaaca tgtccaacca gcgccactgg gaagcaccga tgcaggccta tgtcggcgat ctgctggcgg gcaaggaagg cacgcgcggc aagaacttca acatgcgctg gatcgccagc atggtcgccg acgtgcaccg catcctgacc cgcggcggca tcttcatcta cccgtgggac aagaaggatc cgtccaaggc cggcaagctg cgcctgatgt acgaggccaa cccgatgggc ctgctggtcg aacaggccgg cggcgccgcc tggaccggcc gcgagcgcat cctcgacatc cagcccgacc agctgcacca gcgcgtgccg gtgttcctgg gttcgcgcga ggaagtggcc gaagccgtgc gctaccacca cgcacacgac aacgcacagg gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for StmaA.17936.a.B1.PW38784
| PURIFICATION DATe: | 1/14/2020 |
| CONCENTRATION: | 91mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML TRFLIQEQHA GRINADLRQL IAVVARACTS ISIAVSKGAL GGVLGDAGTG NVQGEAQKKL DVISNEILLE ANAWGGHLAA CASEEMDHSQ PVPDIYPRGD FLLLFDPLDG SSNIDVNVSV GTIFSVLRCP TNVELPGDDA FLQPGSKQIA AGYCIYGPST QLVLTVGHGT HAFTLDREKG EFVLTTENMQ IPAATQEFAI NMSNQRHWEA PMQAYVGDLL AGKEGTRGKN FNMRWIASMV ADVHRILTRG GIFIYPWDKK DPSKAGKLRL MYEANPMGLL VEQAGGAAWT GRERILDIQP DQLHQRVPVF LGSREEVAEA V
Validated NT Sequence
gcgcacggct tcggccactt cctcgcgcga acccaggaac accggcacgc gctggtgcag ctggtcgggc tggatgtcga ggatgcgctc gcggccggtc caggcggcgc cgccggcctg ttcgaccagc aggcccatcg ggttggcctc gtacatcagg cgcagcttgc cggccttgga cggatccttc ttgtcccacg ggtagatgaa gatgccgccg cgggtcagga tgcggtgcac gtcggcgacc atgctggcga tccagcgcat gttgaagttc ttgccgcgcg tgccttcctt gcccgccagc agatcgccga cataggcctg catcggtgct tcccagtggc gctggttgga catgttgatc gcgaactcct gggtagccgc cggaatctgc atgttctcgg tggtcagcac gaactcgccc ttctcacggt ccagggtgaa ggcgtgggtg ccatggccga cggtcagcac cagctgggtg ctgggcccgt agatgcagta gccggcggcg atctgcttgc tgcccggctg caggaaggcg tcgtcgcccg gcagttcgac attggtcggg cagcgcagca ccgagaagat ggtgccgacg gagacgttga cgtcgatgtt ggagctgccg tcgaggggat cgaacagcag caggaaatca ccgcgcgggt agatgtccgg caccggctgg ctgtggtcca tttcctccga ggcgcacgcg gcgaggtggc caccccaggc gttcgcttca agcaggatct cgttgctgat gacgtccagc ttcttctgcg cctcgccctg cacgttaccg gtaccggcat cgcccagcac gccaccgagg gcgcccttgc tgacggcgat ggagatgctg gtgcaggcgc gggcgacgac cgcgatcagc tggcgcaggt cggcgttgat gcggccggcg tgctgttcct ggatcaggaa gcgggtcaac atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMT RFLIQEQHAG RINADLRQLI AVVARACTSI SIAVSKGALG GVLGDAGTGN VQGEAQKKLD VISNEILLEA NAWGGHLAAC ASEEMDHSQP VPDIYPRGDF LLLFDPLDGS SNIDVNVSVG TIFSVLRCPT NVELPGDDAF LQPGSKQIAA GYCIYGPSTQ LVLTVGHGTH AFTLDREKGE FVLTTENMQI PAATQEFAIN MSNQRHWEAP MQAYVGDLLA GKEGTRGKNF NMRWIASMVA DVHRILTRGG IFIYPWDKKD PSKAGKLRLM YEANPMGLLV EQAGGAAWTG RERILDIQPD QLHQRVPVFL GSREEVAEAV RYHHAHDNAQ G
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGACCC GCTTCCTGAT CCAGGAACAG CACGCCGGCC GCATCAACGC CGACCTGCGC CAGCTGATCG CGGTCGTCGC CCGCGCCTGC ACCAGCATCT CCATCGCCGT CAGCAAGGGC GCCCTCGGTG GCGTGCTGGG CGATGCCGGT ACCGGTAACG TGCAGGGCGA GGCGCAGAAG AAGCTGGACG TCATCAGCAA CGAGATCCTG CTTGAAGCGA ACGCCTGGGG TGGCCACCTC GCCGCGTGCG CCTCGGAGGA AATGGACCAC AGCCAGCCGG TGCCGGACAT CTACCCGCGC GGTGATTTCC TGCTGCTGTT CGATCCCCTC GACGGCAGCT CCAACATCGA CGTCAACGTC TCCGTCGGCA CCATCTTCTC GGTGCTGCGC TGCCCGACCA ATGTCGAACT GCCGGGCGAC GACGCCTTCC TGCAGCCGGG CAGCAAGCAG ATCGCCGCCG GCTACTGCAT CTACGGGCCC AGCACCCAGC TGGTGCTGAC CGTCGGCCAT GGCACCCACG CCTTCACCCT GGACCGTGAG AAGGGCGAGT TCGTGCTGAC CACCGAGAAC ATGCAGATTC CGGCGGCTAC CCAGGAGTTC GCGATCAACA TGTCCAACCA GCGCCACTGG GAAGCACCGA TGCAGGCCTA TGTCGGCGAT CTGCTGGCGG GCAAGGAAGG CACGCGCGGC AAGAACTTCA ACATGCGCTG GATCGCCAGC ATGGTCGCCG ACGTGCACCG CATCCTGACC CGCGGCGGCA TCTTCATCTA CCCGTGGGAC AAGAAGGATC CGTCCAAGGC CGGCAAGCTG CGCCTGATGT ACGAGGCCAA CCCGATGGGC CTGCTGGTCG AACAGGCCGG CGGCGCCGCC TGGACCGGCC GCGAGCGCAT CCTCGACATC CAGCCCGACC AGCTGCACCA GCGCGTGCCG GTGTTCCTGG GTTCGCGCGA GGAAGTGGCC GAAGCCGTGC GCTACCACCA CGCACACGAC AACGCACAGG GCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT