StmaA.17935.a

Heat shock protein 60 kDa family chaperone GroEL (groEL)

CENTER ID: StmaA.17935.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17935.a.B1.GE43439 Full length(StmaA.17935.a) 1 549
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3983

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAKDIRFGE DARSRMVRGV NVLANAVKAT LGPKGRNVVL EKSFGAPTIT KDGVSVAKEI ELADKFENMG AQMVKEVASR TNDDAGDGTT TATVLAQALI REGAKAVAAG MNPMDLKRGI DKAVSAAVAE LKNISKPTAD DKAIAQVGTI SANSDESIGQ IIADAMKEVG KEGVITVEEG SGLDNELDVV KGMQFDRGYL SPYFINNQQS QTADLDDPFI LLHDKKISNV RDLLPVLEGV AKAGKPLLIV AEEVEGEALA TLVVNTIRGI VKVVAVKAPG FGDRRKAMLE DMAVLTGGTV ISEEVGLSLE KATIKDLGRA KKVQVSKENT TIIDGVGDKA NVDARVAQIK TQIQDTSSDY DREKLQERVA KLAGGVAVIK VGASTEIEMK EKKDRVDDAL HATRAAVEEG VVPGGGVALV RAITALAGLK GANEDQNHGI QIALRAMEAP LREIVANAGD EPSVIINKVK EGTGSFGYNA ATGEFGDMLQ FGILDPTKVT RSALQNAASI AGLMITTEAM VAEAPKKDEP AMGGAGGMGG MGGMGGMDF
NT Sequence
ATGGCTGCCA AGGATATTCG TTTCGGTGAA GACGCCCGTT CGCGCATGGT GCGCGGCGTC AACGTTCTCG CCAATGCCGT CAAGGCCACC CTGGGCCCGA AGGGCCGCAA CGTCGTGCTG GAAAAGAGCT TCGGCGCCCC GACCATCACC AAGGACGGCG TCTCCGTCGC CAAGGAAATC GAACTGGCTG ACAAGTTCGA GAACATGGGC GCGCAGATGG TGAAGGAAGT TGCTTCGCGC ACCAACGACG ATGCCGGCGA CGGCACCACC ACCGCCACCG TGCTGGCCCA GGCCCTGATC CGCGAAGGCG CCAAGGCCGT TGCCGCCGGC ATGAACCCGA TGGACCTCAA GCGCGGTATC GACAAGGCCG TTTCGGCCGC CGTCGCCGAG CTGAAGAACA TCTCCAAGCC GACCGCCGAC GACAAGGCGA TCGCCCAGGT CGGTACCATC TCGGCCAACT CGGACGAGTC GATCGGCCAG ATCATCGCTG ACGCGATGAA GGAAGTCGGC AAGGAAGGCG TCATCACCGT TGAAGAAGGC TCGGGCCTGG ACAACGAGCT GGACGTGGTC AAGGGCATGC AGTTCGACCG CGGCTACCTG TCGCCGTACT TCATCAACAA CCAGCAGTCG CAGACCGCTG ACCTGGATGA CCCGTTCATC CTGCTGCACG ACAAGAAGAT CTCCAACGTC CGTGACCTGC TGCCGGTGCT GGAAGGCGTC GCCAAGGCCG GCAAGCCGCT GCTGATCGTG GCCGAGGAAG TTGAAGGCGA AGCGCTGGCC ACCCTGGTCG TCAACACCAT CCGTGGCATC GTCAAGGTCG TGGCCGTCAA GGCTCCGGGC TTCGGCGACC GTCGCAAGGC GATGCTGGAA GACATGGCCG TGCTGACCGG CGGCACCGTG ATCTCCGAAG AAGTCGGCCT GTCGCTGGAG AAGGCCACCA TCAAGGACCT GGGCCGCGCC AAGAAGGTGC AGGTCTCCAA GGAGAACACC ACCATCATCG ACGGCGTCGG CGACAAGGCC AACGTTGATG CACGCGTGGC GCAGATCAAG ACCCAGATCC AGGACACCTC CTCGGATTAC GACCGCGAGA AGCTGCAGGA ACGCGTGGCC AAGCTGGCCG GCGGTGTTGC CGTGATCAAG GTCGGCGCCT CGACCGAAAT CGAAATGAAG GAAAAGAAGG ATCGCGTCGA CGACGCCCTG CACGCCACCC GTGCGGCCGT TGAAGAAGGC GTGGTTCCGG GCGGCGGCGT TGCCCTGGTC CGCGCGATCA CCGCGCTGGC CGGCCTGAAG GGCGCCAACG AAGACCAGAA CCACGGCATC CAGATCGCCC TGCGTGCGAT GGAAGCCCCG CTGCGCGAGA TCGTTGCCAA CGCCGGTGAC GAGCCGTCGG TCATCATCAA CAAGGTCAAG GAAGGCACCG GCAGCTTCGG CTACAACGCC GCCACCGGCG AGTTCGGCGA CATGCTGCAG TTCGGCATCC TGGACCCGAC CAAGGTGACC CGTTCGGCGC TGCAGAACGC GGCCTCGATC GCTGGCCTGA TGATCACCAC CGAAGCCATG GTTGCCGAAG CTCCGAAGAA GGACGAGCCG GCCATGGGCG GCGCCGGTGG CATGGGCGGC ATGGGTGGCA TGGGCGGCAT GGACTTC
Details for StmaA.17935.a.B1.GE43439
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA AKDIRFGEDA RSRMVRGVNV LANAVKATLG PKGRNVVLEK SFGAPTITKD GVSVAKEIEL ADKFENMGAQ MVKEVASRTN DDAGDGTTTA TVLAQALIRE GAKAVAAGMN PMDLKRGIDK AVSAAVAELK NISKPTADDK AIAQVGTISA NSDESIGQII ADAMKEVGKE GVITVEEGSG LDNELDVVKG MQFDRGYLSP YFINNQQSQT ADLDDPFILL HDKKISNVRD LLPVLEGVAK AGKPLLIVAE EVEGEALATL VVNTIRGIVK VVAVKAPGFG DRRKAMLEDM AVLTGGTVIS EEVGLSLEKA TIKDLGRAKK VQVSKENTTI IDGVGDKANV DARVAQIKTQ IQDTSSDYDR EKLQERVAKL AGGVAVIKVG ASTEIEMKEK KDRVDDALHA TRAAVEEGVV PGGGVALVRA ITALAGLKGA NEDQNHGIQI ALRAMEAPLR EIVANAGDEP SVIINKVKEG TGSFGYNAAT GEFGDMLQFG ILDPTKVTRS ALQNAASIAG LMITTEAMVA EAPKKDEPAM GGAGGMGGMG GMGGMDF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg ccaaggatat tcgtttcggt gaagacgccc gttcgcgcat ggtgcgcggc gtcaacgttc tcgccaatgc cgtcaaggcc accctgggcc cgaagggccg caacgtcgtg ctggaaaaga gcttcggcgc cccgaccatc accaaggacg gcgtctccgt cgccaaggaa atcgaactgg ctgacaagtt cgagaacatg ggcgcgcaga tggtgaagga agttgcttcg cgcaccaacg acgatgccgg cgacggcacc accaccgcca ccgtgctggc ccaggccctg atccgcgaag gcgccaaggc cgttgccgcc ggcatgaacc cgatggacct caagcgcggt atcgacaagg ccgtttcggc cgccgtcgcc gagctgaaga acatctccaa gccgaccgcc gacgacaagg cgatcgccca ggtcggtacc atctcggcca actcggacga gtcgatcggc cagatcatcg ctgacgcgat gaaggaagtc ggcaaggaag gcgtcatcac cgttgaagaa ggctcgggcc tggacaacga gctggacgtg gtcaagggca tgcagttcga ccgcggctac ctgtcgccgt acttcatcaa caaccagcag tcgcagaccg ctgacctgga tgacccgttc atcctgctgc acgacaagaa gatctccaac gtccgtgacc tgctgccggt gctggaaggc gtcgccaagg ccggcaagcc gctgctgatc gtggccgagg aagttgaagg cgaagcgctg gccaccctgg tcgtcaacac catccgtggc atcgtcaagg tcgtggccgt caaggctccg ggcttcggcg accgtcgcaa ggcgatgctg gaagacatgg ccgtgctgac cggcggcacc gtgatctccg aagaagtcgg cctgtcgctg gagaaggcca ccatcaagga cctgggccgc gccaagaagg tgcaggtctc caaggagaac accaccatca tcgacggcgt cggcgacaag gccaacgttg atgcacgcgt ggcgcagatc aagacccaga tccaggacac ctcctcggat tacgaccgcg agaagctgca ggaacgcgtg gccaagctgg ccggcggtgt tgccgtgatc aaggtcggcg cctcgaccga aatcgaaatg aaggaaaaga aggatcgcgt cgacgacgcc ctgcacgcca cccgtgcggc cgttgaagaa ggcgtggttc cgggcggcgg cgttgccctg gtccgcgcga tcaccgcgct ggccggcctg aagggcgcca acgaagacca gaaccacggc atccagatcg ccctgcgtgc gatggaagcc ccgctgcgcg agatcgttgc caacgccggt gacgagccgt cggtcatcat caacaaggtc aaggaaggca ccggcagctt cggctacaac gccgccaccg gcgagttcgg cgacatgctg cagttcggca tcctggaccc gaccaaggtg acccgttcgg cgctgcagaa cgcggcctcg atcgctggcc tgatgatcac caccgaagcc atggttgccg aagctccgaa gaaggacgag ccggccatgg gcggcgccgg tggcatgggc ggcatgggtg gcatgggcgg catggacttc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat