StmaA.17926.b

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) (glmS)

CENTER ID: StmaA.17926.b
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17926.b.B1.GE43436 Full length(StmaA.17926.b) 1 612
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17926.b.B1.PW38776 Full length(StmaA.17926.b) 1 612
StmaA.17926.b.B1.PW38777 Full length(StmaA.17926.b) 1 612

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3871

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCGIVGAIAD RDVVPVLIEG LKRLEYRGYD SSGIAVIDQG ERRDVRRVRR TGRVSEMATA AEAEGFNAVL GIGHTRWATH GGVTEANAHP HISHGVALVH NGIIENHEEQ REKLRALGYT FESQTDTEVI AHLIHHHLKS GDDLLVALQH TVKELTGAYA LAVVSRAEPE RFVCARMGCP LLIGLGEGEN FVASDVSAVI SATRKVIFLE EGDTAEIRRD GVRIFDEHDR PIERDVHLSD VSLASLELGP YRHFMQKEIH EQPRALGDTI EAAIDAGGFP AGLFGKNAEA VLSGIEGVQI IACGTSYYAG LTARYWIEAI AGLPCSVEIA SEYRYRAAYA NPKHLIVTIS QSGETLDTME ALKYAKSLGH KHTLSICNVP ESAIPRASEL VCYTRAGAEI GVASTKAFTT QLAALFQLTV VLGKLHGRID AAQEADYLEQ LRFLPGSVQH ALNMEPQIAA WAERFARKSS ALFLGRGLHY PIALEGALKL KEISYIHAEA YPAGELKHGP LALVDEDMPV VVIAPNDSLL EKVKSNMQEV RARGGELFVF ADQDSNFNES EGVHVIRTPR HAGVLSPIVH TIPVQLLAYH TALARGTDVD KPRNLAKSVT VE
NT Sequence
ATGTGTGGAA TCGTGGGTGC GATCGCTGAT CGCGACGTAG TGCCGGTGCT GATCGAAGGC CTGAAGCGGC TGGAGTACCG TGGTTACGAT TCCTCCGGCA TCGCGGTGAT CGACCAGGGT GAGCGCCGCG ATGTACGTCG CGTGCGTCGT ACCGGTCGTG TGTCGGAAAT GGCCACGGCG GCCGAGGCCG AGGGCTTCAA CGCGGTGCTG GGCATCGGCC ACACCCGCTG GGCCACCCAT GGGGGCGTCA CCGAGGCCAA CGCGCATCCG CACATCAGCC ATGGCGTGGC GCTGGTGCAC AACGGCATCA TCGAGAACCA CGAAGAGCAG CGCGAGAAGC TGCGTGCGCT GGGCTACACC TTCGAGTCGC AGACCGACAC CGAAGTCATC GCACACCTGA TCCACCATCA CCTGAAGAGT GGTGACGACC TGCTGGTGGC GCTGCAGCAT ACGGTGAAGG AGCTGACCGG TGCCTACGCA CTGGCCGTGG TCAGCCGCGC CGAGCCGGAA CGCTTCGTCT GCGCGCGGAT GGGCTGCCCG CTGCTGATCG GCCTGGGCGA GGGCGAGAAC TTCGTTGCTT CCGACGTCTC GGCGGTGATC TCGGCCACCC GCAAGGTCAT TTTCCTGGAA GAGGGCGATA CCGCCGAGAT CCGTCGCGAT GGCGTGCGCA TCTTCGACGA GCACGACCGG CCGATCGAGC GCGACGTGCA TCTGTCCGAT GTGTCGCTGG CATCGCTGGA GCTGGGCCCG TACCGCCACT TCATGCAGAA GGAGATCCAC GAGCAGCCGC GCGCGCTGGG CGACACCATC GAGGCGGCGA TCGACGCCGG TGGTTTCCCG GCCGGGCTGT TCGGCAAGAA CGCCGAAGCG GTGCTGTCGG GCATCGAGGG TGTGCAGATC ATCGCCTGCG GCACCAGCTA CTACGCCGGC CTGACCGCGC GCTACTGGAT CGAAGCCATC GCCGGCCTTC CGTGCAGCGT GGAGATCGCC AGCGAGTACC GCTATCGCGC GGCCTACGCG AACCCGAAGC ATCTGATCGT CACCATCTCC CAGTCCGGCG AAACGCTGGA TACGATGGAA GCGCTGAAGT ACGCCAAGTC GCTCGGCCAC AAGCACACGC TGTCGATCTG CAACGTGCCG GAAAGTGCGA TTCCGCGCGC CAGCGAACTG GTGTGCTACA CCCGTGCCGG TGCCGAGATC GGCGTGGCCT CGACCAAGGC CTTCACCACC CAGCTGGCGG CACTGTTCCA GCTGACCGTG GTGCTGGGCA AGCTGCACGG CCGCATCGAC GCGGCGCAGG AAGCGGACTA CCTCGAGCAG CTGCGCTTCC TGCCGGGCAG CGTGCAGCAC GCATTGAACA TGGAGCCGCA GATCGCTGCC TGGGCCGAGC GTTTCGCACG CAAGAGCAGC GCGCTGTTCC TGGGCCGTGG CCTGCATTAC CCGATCGCGC TGGAAGGCGC GCTCAAGCTC AAGGAAATCT CCTACATCCA CGCCGAGGCC TACCCGGCAG GCGAGCTGAA GCATGGCCCG CTGGCGCTGG TGGACGAGGA CATGCCGGTG GTGGTGATCG CGCCGAACGA CAGCCTGCTG GAGAAGGTGA AGTCGAACAT GCAGGAAGTG CGCGCGCGTG GCGGTGAACT GTTCGTGTTC GCCGACCAGG ACAGCAACTT CAACGAGTCC GAAGGCGTGC ACGTGATCCG TACGCCGCGT CATGCCGGCG TGCTCAGCCC GATCGTGCAC ACCATTCCGG TGCAGTTGCT GGCCTACCAC ACCGCGCTGG CGCGCGGCAC CGACGTGGAT AAGCCGCGCA ACCTGGCCAA GAGCGTGACG GTGGAG
Details for StmaA.17926.b.B1.GE43436
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMC GIVGAIADRD VVPVLIEGLK RLEYRGYDSS GIAVIDQGER RDVRRVRRTG RVSEMATAAE AEGFNAVLGI GHTRWATHGG VTEANAHPHI SHGVALVHNG IIENHEEQRE KLRALGYTFE SQTDTEVIAH LIHHHLKSGD DLLVALQHTV KELTGAYALA VVSRAEPERF VCARMGCPLL IGLGEGENFV ASDVSAVISA TRKVIFLEEG DTAEIRRDGV RIFDEHDRPI ERDVHLSDVS LASLELGPYR HFMQKEIHEQ PRALGDTIEA AIDAGGFPAG LFGKNAEAVL SGIEGVQIIA CGTSYYAGLT ARYWIEAIAG LPCSVEIASE YRYRAAYANP KHLIVTISQS GETLDTMEAL KYAKSLGHKH TLSICNVPES AIPRASELVC YTRAGAEIGV ASTKAFTTQL AALFQLTVVL GKLHGRIDAA QEADYLEQLR FLPGSVQHAL NMEPQIAAWA ERFARKSSAL FLGRGLHYPI ALEGALKLKE ISYIHAEAYP AGELKHGPLA LVDEDMPVVV IAPNDSLLEK VKSNMQEVRA RGGELFVFAD QDSNFNESEG VHVIRTPRHA GVLSPIVHTI PVQLLAYHTA LARGTDVDKP RNLAKSVTVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtg gaatcgtggg tgcgatcgct gatcgcgacg tagtgccggt gctgatcgaa ggcctgaagc ggctggagta ccgtggttac gattcctccg gcatcgcggt gatcgaccag ggtgagcgcc gcgatgtacg tcgcgtgcgt cgtaccggtc gtgtgtcgga aatggccacg gcggccgagg ccgagggctt caacgcggtg ctgggcatcg gccacacccg ctgggccacc catgggggcg tcaccgaggc caacgcgcat ccgcacatca gccatggcgt ggcgctggtg cacaacggca tcatcgagaa ccacgaagag cagcgcgaga agctgcgtgc gctgggctac accttcgagt cgcagaccga caccgaagtc atcgcacacc tgatccacca tcacctgaag agtggtgacg acctgctggt ggcgctgcag catacggtga aggagctgac cggtgcctac gcactggccg tggtcagccg cgccgagccg gaacgcttcg tctgcgcgcg gatgggctgc ccgctgctga tcggcctggg cgagggcgag aacttcgttg cttccgacgt ctcggcggtg atctcggcca cccgcaaggt cattttcctg gaagagggcg ataccgccga gatccgtcgc gatggcgtgc gcatcttcga cgagcacgac cggccgatcg agcgcgacgt gcatctgtcc gatgtgtcgc tggcatcgct ggagctgggc ccgtaccgcc acttcatgca gaaggagatc cacgagcagc cgcgcgcgct gggcgacacc atcgaggcgg cgatcgacgc cggtggtttc ccggccgggc tgttcggcaa gaacgccgaa gcggtgctgt cgggcatcga gggtgtgcag atcatcgcct gcggcaccag ctactacgcc ggcctgaccg cgcgctactg gatcgaagcc atcgccggcc ttccgtgcag cgtggagatc gccagcgagt accgctatcg cgcggcctac gcgaacccga agcatctgat cgtcaccatc tcccagtccg gcgaaacgct ggatacgatg gaagcgctga agtacgccaa gtcgctcggc cacaagcaca cgctgtcgat ctgcaacgtg ccggaaagtg cgattccgcg cgccagcgaa ctggtgtgct acacccgtgc cggtgccgag atcggcgtgg cctcgaccaa ggccttcacc acccagctgg cggcactgtt ccagctgacc gtggtgctgg gcaagctgca cggccgcatc gacgcggcgc aggaagcgga ctacctcgag cagctgcgct tcctgccggg cagcgtgcag cacgcattga acatggagcc gcagatcgct gcctgggccg agcgtttcgc acgcaagagc agcgcgctgt tcctgggccg tggcctgcat tacccgatcg cgctggaagg cgcgctcaag ctcaaggaaa tctcctacat ccacgccgag gcctacccgg caggcgagct gaagcatggc ccgctggcgc tggtggacga ggacatgccg gtggtggtga tcgcgccgaa cgacagcctg ctggagaagg tgaagtcgaa catgcaggaa gtgcgcgcgc gtggcggtga actgttcgtg ttcgccgacc aggacagcaa cttcaacgag tccgaaggcg tgcacgtgat ccgtacgccg cgtcatgccg gcgtgctcag cccgatcgtg cacaccattc cggtgcagtt gctggcctac cacaccgcgc tggcgcgcgg caccgacgtg gataagccgc gcaacctggc caagagcgtg acggtggagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for StmaA.17926.b.B1.PW38776
PURIFICATION DATe: 12/17/2019
CONCENTRATION: 11mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMC GIVGAIADRD VVPVLIEGLK RLEYRGYDSS GIAVIDQGER RDVRRVRRTG RVSEMATAAE AEGFNAVLGI GHTRWATHGG VTEANAHPHI SHGVALVHNG IIENHEEQRE KLRALGYTFE SQTDTEVIAH LIHHHLKSGD DLLVALQHTV KELTGAYALA VVSRAEPERF VCARMGCPLL IGLGEGENFV ASDVSAVISA TRKVIFLEEG DTAEIRRDGV RIFDEHDRPI ERDVHLSDVS LASLELGPYR HFMQKEIHEQ PRALGDTIEA AIDAGGFPAG LFGKNAEAVL SGIEGVQIIA CGTSYYAGLT ARYWIEAIAG LPCSVEIASE YRYRAAYANP KHLIVTISQS GETLDTMEAL KYAKSLGHKH TLSICNVPES AIPRASELVC YTRAGAEIGV ASTKAFTTQL AALFQLTVVL GKLHGRIDAA QEADYLEQLR FLPGSVQHAL NMEPQIAAWA ERFARKSSAL FLGRGLHYPI ALEGALKLKE ISYIHAEAYP AGELKHGPLA LVDEDMPVVV IAPNDSLLEK VKSNMQEVRA RGGELFVFAD QDSNFNESEG VHVIRTPRHA GVLSPIVHTI PVQLLAYHTA LARGTDVDKP RNLAKSVTVE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGTG GAATCGTGGG TGCGATCGCT GATCGCGACG TAGTGCCGGT GCTGATCGAA GGCCTGAAGC GGCTGGAGTA CCGTGGTTAC GATTCCTCCG GCATCGCGGT GATCGACCAG GGTGAGCGCC GCGATGTACG TCGCGTGCGT CGTACCGGTC GTGTGTCGGA AATGGCCACG GCGGCCGAGG CCGAGGGCTT CAACGCGGTG CTGGGCATCG GCCACACCCG CTGGGCCACC CATGGGGGCG TCACCGAGGC CAACGCGCAT CCGCACATCA GCCATGGCGT GGCGCTGGTG CACAACGGCA TCATCGAGAA CCACGAAGAG CAGCGCGAGA AGCTGCGTGC GCTGGGCTAC ACCTTCGAGT CGCAGACCGA CACCGAAGTC ATCGCACACC TGATCCACCA TCACCTGAAG AGTGGTGACG ACCTGCTGGT GGCGCTGCAG CATACGGTGA AGGAGCTGAC CGGTGCCTAC GCACTGGCCG TGGTCAGCCG CGCCGAGCCG GAACGCTTCG TCTGCGCGCG GATGGGCTGC CCGCTGCTGA TCGGCCTGGG CGAGGGCGAG AACTTCGTTG CTTCCGACGT CTCGGCGGTG ATCTCGGCCA CCCGCAAGGT CATTTTCCTG GAAGAGGGCG ATACCGCCGA GATCCGTCGC GATGGCGTGC GCATCTTCGA CGAGCACGAC CGGCCGATCG AGCGCGACGT GCATCTGTCC GATGTGTCGC TGGCATCGCT GGAGCTGGGC CCGTACCGCC ACTTCATGCA GAAGGAGATC CACGAGCAGC CGCGCGCGCT GGGCGACACC ATCGAGGCGG CGATCGACGC CGGTGGTTTC CCGGCCGGGC TGTTCGGCAA GAACGCCGAA GCGGTGCTGT CGGGCATCGA GGGTGTGCAG ATCATCGCCT GCGGCACCAG CTACTACGCC GGCCTGACCG CGCGCTACTG GATCGAAGCC ATCGCCGGCC TTCCGTGCAG CGTGGAGATC GCCAGCGAGT ACCGCTATCG CGCGGCCTAC GCGAACCCGA AGCATCTGAT CGTCACCATC TCCCAGTCCG GCGAAACGCT GGATACGATG GAAGCGCTGA AGTACGCCAA GTCGCTCGGC CACAAGCACA CGCTGTCGAT CTGCAACGTG CCGGAAAGTG CGATTCCGCG CGCCAGCGAA CTGGTGTGCT ACACCCGTGC CGGTGCCGAG ATCGGCGTGG CCTCGACCAA GGCCTTCACC ACCCAGCTGG CGGCACTGTT CCAGCTGACC GTGGTGCTGG GCAAGCTGCA CGGCCGCATC GACGCGGCGC AGGAAGCGGA CTACCTCGAG CAGCTGCGCT TCCTGCCGGG CAGCGTGCAG CACGCATTGA ACATGGAGCC GCAGATCGCT GCCTGGGCCG AGCGTTTCGC ACGCAAGAGC AGCGCGCTGT TCCTGGGCCG TGGCCTGCAT TACCCGATCG CGCTGGAAGG CGCGCTCAAG CTCAAGGAAA TCTCCTACAT CCACGCCGAG GCCTACCCGG CAGGCGAGCT GAAGCATGGC CCGCTGGCGC TGGTGGACGA GGACATGCCG GTGGTGGTGA TCGCGCCGAA CGACAGCCTG CTGGAGAAGG TGAAGTCGAA CATGCAGGAA GTGCGCGCGC GTGGCGGTGA ACTGTTCGTG TTCGCCGACC AGGACAGCAA CTTCAACGAG TCCGAAGGCG TGCACGTGAT CCGTACGCCG CGTCATGCCG GCGTGCTCAG CCCGATCGTG CACACCATTC CGGTGCAGTT GCTGGCCTAC CACACCGCGC TGGCGCGCGG CACCGACGTG GATAAGCCGC GCAACCTGGC CAAGAGCGTG ACGGTGGAGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for StmaA.17926.b.B1.PW38777
PURIFICATION DATe: 12/17/2019
CONCENTRATION: 12mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMC GIVGAIADRD VVPVLIEGLK RLEYRGYDSS GIAVIDQGER RDVRRVRRTG RVSEMATAAE AEGFNAVLGI GHTRWATHGG VTEANAHPHI SHGVALVHNG IIENHEEQRE KLRALGYTFE SQTDTEVIAH LIHHHLKSGD DLLVALQHTV KELTGAYALA VVSRAEPERF VCARMGCPLL IGLGEGENFV ASDVSAVISA TRKVIFLEEG DTAEIRRDGV RIFDEHDRPI ERDVHLSDVS LASLELGPYR HFMQKEIHEQ PRALGDTIEA AIDAGGFPAG LFGKNAEAVL SGIEGVQIIA CGTSYYAGLT ARYWIEAIAG LPCSVEIASE YRYRAAYANP KHLIVTISQS GETLDTMEAL KYAKSLGHKH TLSICNVPES AIPRASELVC YTRAGAEIGV ASTKAFTTQL AALFQLTVVL GKLHGRIDAA QEADYLEQLR FLPGSVQHAL NMEPQIAAWA ERFARKSSAL FLGRGLHYPI ALEGALKLKE ISYIHAEAYP AGELKHGPLA LVDEDMPVVV IAPNDSLLEK VKSNMQEVRA RGGELFVFAD QDSNFNESEG VHVIRTPRHA GVLSPIVHTI PVQLLAYHTA LARGTDVDKP RNLAKSVTVE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGTG GAATCGTGGG TGCGATCGCT GATCGCGACG TAGTGCCGGT GCTGATCGAA GGCCTGAAGC GGCTGGAGTA CCGTGGTTAC GATTCCTCCG GCATCGCGGT GATCGACCAG GGTGAGCGCC GCGATGTACG TCGCGTGCGT CGTACCGGTC GTGTGTCGGA AATGGCCACG GCGGCCGAGG CCGAGGGCTT CAACGCGGTG CTGGGCATCG GCCACACCCG CTGGGCCACC CATGGGGGCG TCACCGAGGC CAACGCGCAT CCGCACATCA GCCATGGCGT GGCGCTGGTG CACAACGGCA TCATCGAGAA CCACGAAGAG CAGCGCGAGA AGCTGCGTGC GCTGGGCTAC ACCTTCGAGT CGCAGACCGA CACCGAAGTC ATCGCACACC TGATCCACCA TCACCTGAAG AGTGGTGACG ACCTGCTGGT GGCGCTGCAG CATACGGTGA AGGAGCTGAC CGGTGCCTAC GCACTGGCCG TGGTCAGCCG CGCCGAGCCG GAACGCTTCG TCTGCGCGCG GATGGGCTGC CCGCTGCTGA TCGGCCTGGG CGAGGGCGAG AACTTCGTTG CTTCCGACGT CTCGGCGGTG ATCTCGGCCA CCCGCAAGGT CATTTTCCTG GAAGAGGGCG ATACCGCCGA GATCCGTCGC GATGGCGTGC GCATCTTCGA CGAGCACGAC CGGCCGATCG AGCGCGACGT GCATCTGTCC GATGTGTCGC TGGCATCGCT GGAGCTGGGC CCGTACCGCC ACTTCATGCA GAAGGAGATC CACGAGCAGC CGCGCGCGCT GGGCGACACC ATCGAGGCGG CGATCGACGC CGGTGGTTTC CCGGCCGGGC TGTTCGGCAA GAACGCCGAA GCGGTGCTGT CGGGCATCGA GGGTGTGCAG ATCATCGCCT GCGGCACCAG CTACTACGCC GGCCTGACCG CGCGCTACTG GATCGAAGCC ATCGCCGGCC TTCCGTGCAG CGTGGAGATC GCCAGCGAGT ACCGCTATCG CGCGGCCTAC GCGAACCCGA AGCATCTGAT CGTCACCATC TCCCAGTCCG GCGAAACGCT GGATACGATG GAAGCGCTGA AGTACGCCAA GTCGCTCGGC CACAAGCACA CGCTGTCGAT CTGCAACGTG CCGGAAAGTG CGATTCCGCG CGCCAGCGAA CTGGTGTGCT ACACCCGTGC CGGTGCCGAG ATCGGCGTGG CCTCGACCAA GGCCTTCACC ACCCAGCTGG CGGCACTGTT CCAGCTGACC GTGGTGCTGG GCAAGCTGCA CGGCCGCATC GACGCGGCGC AGGAAGCGGA CTACCTCGAG CAGCTGCGCT TCCTGCCGGG CAGCGTGCAG CACGCATTGA ACATGGAGCC GCAGATCGCT GCCTGGGCCG AGCGTTTCGC ACGCAAGAGC AGCGCGCTGT TCCTGGGCCG TGGCCTGCAT TACCCGATCG CGCTGGAAGG CGCGCTCAAG CTCAAGGAAA TCTCCTACAT CCACGCCGAG GCCTACCCGG CAGGCGAGCT GAAGCATGGC CCGCTGGCGC TGGTGGACGA GGACATGCCG GTGGTGGTGA TCGCGCCGAA CGACAGCCTG CTGGAGAAGG TGAAGTCGAA CATGCAGGAA GTGCGCGCGC GTGGCGGTGA ACTGTTCGTG TTCGCCGACC AGGACAGCAA CTTCAACGAG TCCGAAGGCG TGCACGTGAT CCGTACGCCG CGTCATGCCG GCGTGCTCAG CCCGATCGTG CACACCATTC CGGTGCAGTT GCTGGCCTAC CACACCGCGC TGGCGCGCGG CACCGACGTG GATAAGCCGC GCAACCTGGC CAAGAGCGTG ACGGTGGAGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT