StmaA.17926.a
Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6)
| CENTER ID: | StmaA.17926.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.17926.a.B1.GE43435 | Full length(StmaA.17926.a) | 1 | 342 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.17926.a.B1.PW38775 | Full length(StmaA.17926.a) | 1 | 342 |
Structures
7KQA
DEPOSITED: 11/14/2020
DETERMINATION: XRay
CLONE: StmaA.17926.a.B1.GE43435
PROTEIN: StmaA.17926.a.B1.PW38775
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3792 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLSDPTSTL MFAEAAEAAD VVARQFSRNH ATMETLAASL RAAPPPFVVT CARGSSDHAA TYGKYLLETQ LGLVVASASP SVGSVYAAPL QLRGALFIVI SQSGKSPDLL RNAEAAKAAG ARVIALVNVE DSPLAQLADT VIPLHAGAEK SVAATKSYLA SLAALLQLAA YWKQDSSLRA ALDLLPDALR EAWQCDWSAV TEGLVEATNL FVLGRGLGLG AAQEAALKFK ETCSLHAEAY SSAEVKHGPM ALVDRGFPVL AFAQPDETGA GTRAVVEEFT ARGAQVWMAG AGGNLPVAAA PHPLCAPLLT VQSFYRAINA LALRRGFNPD LPPHLNKVTE TV
NT Sequence
ATGTCGCTGT CAGACCCCAC GTCCACCCTG ATGTTCGCCG AGGCCGCTGA AGCGGCCGAC GTGGTCGCGC GCCAGTTCTC GCGCAACCAC GCCACCATGG AAACCCTGGC CGCCAGCCTG CGTGCTGCAC CGCCGCCGTT CGTGGTCACC TGTGCACGCG GCAGCTCCGA CCACGCCGCC ACCTATGGCA AGTATCTGCT GGAAACCCAG CTGGGCCTGG TGGTCGCGTC GGCCTCGCCA TCGGTGGGGT CGGTCTACGC CGCACCATTG CAGCTGCGCG GCGCACTGTT CATCGTCATC TCGCAGTCGG GCAAGAGCCC GGACCTGCTG CGCAACGCCG AGGCCGCCAA GGCGGCCGGT GCGCGCGTGA TCGCGCTGGT CAACGTCGAG GATTCACCGC TGGCGCAGCT GGCCGACACG GTCATTCCGC TGCACGCGGG TGCCGAAAAG AGCGTGGCGG CGACCAAGAG TTACCTGGCG TCGCTGGCCG CGCTGCTGCA GCTGGCCGCG TACTGGAAGC AGGACAGCAG CCTGCGTGCG GCGCTGGACC TGCTGCCCGA TGCGCTGCGT GAGGCGTGGC AGTGCGACTG GAGCGCGGTG ACCGAAGGCC TGGTCGAGGC CACCAACCTG TTCGTGCTCG GCCGTGGCCT TGGCCTGGGG GCGGCGCAGG AAGCGGCGCT GAAGTTCAAG GAAACCTGCA GCCTGCATGC CGAAGCCTAC AGCTCGGCTG AAGTGAAGCA TGGCCCGATG GCGCTGGTCG ACCGCGGCTT CCCGGTGCTG GCCTTTGCCC AGCCGGATGA AACCGGCGCC GGCACCCGTG CCGTCGTCGA AGAATTCACC GCGCGTGGCG CACAGGTATG GATGGCCGGT GCTGGCGGCA ACCTGCCGGT GGCTGCTGCG CCGCACCCGC TGTGCGCACC GCTGCTGACC GTGCAGAGCT TCTACCGCGC GATCAATGCG CTGGCGCTGC GTCGTGGCTT CAACCCGGAC CTGCCGCCGC ACCTGAACAA GGTGACGGAG ACCGTG
Details for StmaA.17926.a.B1.GE43435
| HARVESTED ON: | 9/18/2019 |
| SEQUENCED ON: | 10/1/2019 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS LSDPTSTLMF AEAAEAADVV ARQFSRNHAT METLAASLRA APPPFVVTCA RGSSDHAATY GKYLLETQLG LVVASASPSV GSVYAAPLQL RGALFIVISQ SGKSPDLLRN AEAAKAAGAR VIALVNVEDS PLAQLADTVI PLHAGAEKSV AATKSYLASL AALLQLAAYW KQDSSLRAAL DLLPDALREA WQCDWSAVTE GLVEATNLFV LGRGLGLGAA QEAALKFKET CSLHAEAYSS AEVKHGPMAL VDRGFPVLAF AQPDETGAGT RAVVEEFTAR GAQVWMAGAG GNLPVAAAPH PLCAPLLTVQ SFYRAINALA LRRGFNPDLP X
Validated NT Sequence
gngnnncggc aggtccgggt tgaagccacg acgcagcgcc agcgcattga tcgcgcggta gaagctctgc acggtcagca gcggtgcgca cagcgggtgc ggcgcagcag ccaccggcag gttgccgcca gcaccggcca tccatacctg tgcgccacgc gcggtgaatt cttcgacgac ggcacgggtg ccggcgccgg tttcatccgg ctgggcaaag gccagcaccg ggaagccgcg gtcgaccagc gccatcgggc catgcttcac ttcagccgag ctgtaggctt cggcatgcag gctgcaggtt tccttgaact tcagcgccgc ttcctgcgcc gcccccaggc caaggccacg gccgagcacg aacaggttgg tggcctcgac caggccttcg gtcaccgcgc tccagtcgca ctgccacgcc tcacgcagcg catcgggcag caggtccagc gccgcacgca ggctgctgtc ctgcttccag tacgcggcca gctgcagcag cgcggccagc gacgccaggt aactcttggt cgccgccacg ctcttttcgg cacccgcgtg cagcggaatg accgtgtcgg ccagctgcgc cagcggtgaa tcctcgacgt tgaccagcgc gatcacgcgc gcaccggccg ccttggcggc ctcggcgttg cgcagcaggt ccgggctctt gcccgactgc gagatgacga tgaacagtgc gccgcgcagc tgcaatggtg cggcgtagac cgaccccacc gatggcgagg ccgacgcgac caccaggccc agctgggttt ccagcagata cttgccatag gtggcggcgt ggtcggagct gccgcgtgca caggtgacca cgaacggcgg cggtgcagca cgcaggctgg cggccagggt ttccatggtg gcgtggttgc gcgagaactg gcgcgcgacc acgtcggccg cttcagcggc ctcggcgaac atcagggtgg acgtggggtc tgacagcgac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMS LSDPTSTLMF AEAAEAADVV ARQFSRNHAT METLAASLRA APPPFVVTCA RGSSDHAATY GKYLLETQLG LVVASASPSV GSVYAAPLQL RGALFIVISQ SGKSPDLLRN AEAAKAAGAR VIALVNVEDS PLAQLADTVI PLHAGAEKSV AATKSYLASL AALLQLAAYW KQDSSLRAAL DLLPDALREA WQCDWSAVTE GLVEATNLFV LGRGLGLGAA QEAALKFKET CSLHAEAYSS AEVKHGPMAL VDRGFPVLAF AQPDETGAGT RAVVEEFTAR GAQVWMAGAG GNLPVAAAPH PLCAPLLTVQ SFYRAINALA LRRGFNPDLP PHLNKVTETV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc tgtcagaccc cacgtccacc ctgatgttcg ccgaggccgc tgaagcggcc gacgtggtcg cgcgccagtt ctcgcgcaac cacgccacca tggaaaccct ggccgccagc ctgcgtgctg caccgccgcc gttcgtggtc acctgtgcac gcggcagctc cgaccacgcc gccacctatg gcaagtatct gctggaaacc cagctgggcc tggtggtcgc gtcggcctcg ccatcggtgg ggtcggtcta cgccgcacca ttgcagctgc gcggcgcact gttcatcgtc atctcgcagt cgggcaagag cccggacctg ctgcgcaacg ccgaggccgc caaggcggcc ggtgcgcgcg tgatcgcgct ggtcaacgtc gaggattcac cgctggcgca gctggccgac acggtcattc cgctgcacgc gggtgccgaa aagagcgtgg cggcgaccaa gagttacctg gcgtcgctgg ccgcgctgct gcagctggcc gcgtactgga agcaggacag cagcctgcgt gcggcgctgg acctgctgcc cgatgcgctg cgtgaggcgt ggcagtgcga ctggagcgcg gtgaccgaag gcctggtcga ggccaccaac ctgttcgtgc tcggccgtgg ccttggcctg ggggcggcgc aggaagcggc gctgaagttc aaggaaacct gcagcctgca tgccgaagcc tacagctcgg ctgaagtgaa gcatggcccg atggcgctgg tcgaccgcgg cttcccggtg ctggcctttg cccagccgga tgaaaccggc gccggcaccc gtgccgtcgt cgaagaattc accgcgcgtg gcgcacaggt atggatggcc ggtgctggcg gcaacctgcc ggtggctgct gcgccgcacc cgctgtgcgc accgctgctg accgtgcaga gcttctaccg cgcgatcaat gcgctggcgc tgcgtcgtgg cttcaacccg gacctgccgc cgcacctgaa caaggtgacg gagaccgtgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for StmaA.17926.a.B1.PW38775
| PURIFICATION DATe: | 12/17/2019 |
| CONCENTRATION: | 100mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS LSDPTSTLMF AEAAEAADVV ARQFSRNHAT METLAASLRA APPPFVVTCA RGSSDHAATY GKYLLETQLG LVVASASPSV GSVYAAPLQL RGALFIVISQ SGKSPDLLRN AEAAKAAGAR VIALVNVEDS PLAQLADTVI PLHAGAEKSV AATKSYLASL AALLQLAAYW KQDSSLRAAL DLLPDALREA WQCDWSAVTE GLVEATNLFV LGRGLGLGAA QEAALKFKET CSLHAEAYSS AEVKHGPMAL VDRGFPVLAF AQPDETGAGT RAVVEEFTAR GAQVWMAGAG GNLPVAAAPH PLCAPLLTVQ SFYRAINALA LRRGFNPDLP X
Validated NT Sequence
gngnnncggc aggtccgggt tgaagccacg acgcagcgcc agcgcattga tcgcgcggta gaagctctgc acggtcagca gcggtgcgca cagcgggtgc ggcgcagcag ccaccggcag gttgccgcca gcaccggcca tccatacctg tgcgccacgc gcggtgaatt cttcgacgac ggcacgggtg ccggcgccgg tttcatccgg ctgggcaaag gccagcaccg ggaagccgcg gtcgaccagc gccatcgggc catgcttcac ttcagccgag ctgtaggctt cggcatgcag gctgcaggtt tccttgaact tcagcgccgc ttcctgcgcc gcccccaggc caaggccacg gccgagcacg aacaggttgg tggcctcgac caggccttcg gtcaccgcgc tccagtcgca ctgccacgcc tcacgcagcg catcgggcag caggtccagc gccgcacgca ggctgctgtc ctgcttccag tacgcggcca gctgcagcag cgcggccagc gacgccaggt aactcttggt cgccgccacg ctcttttcgg cacccgcgtg cagcggaatg accgtgtcgg ccagctgcgc cagcggtgaa tcctcgacgt tgaccagcgc gatcacgcgc gcaccggccg ccttggcggc ctcggcgttg cgcagcaggt ccgggctctt gcccgactgc gagatgacga tgaacagtgc gccgcgcagc tgcaatggtg cggcgtagac cgaccccacc gatggcgagg ccgacgcgac caccaggccc agctgggttt ccagcagata cttgccatag gtggcggcgt ggtcggagct gccgcgtgca caggtgacca cgaacggcgg cggtgcagca cgcaggctgg cggccagggt ttccatggtg gcgtggttgc gcgagaactg gcgcgcgacc acgtcggccg cttcagcggc ctcggcgaac atcagggtgg acgtggggtc tgacagcgac atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMS LSDPTSTLMF AEAAEAADVV ARQFSRNHAT METLAASLRA APPPFVVTCA RGSSDHAATY GKYLLETQLG LVVASASPSV GSVYAAPLQL RGALFIVISQ SGKSPDLLRN AEAAKAAGAR VIALVNVEDS PLAQLADTVI PLHAGAEKSV AATKSYLASL AALLQLAAYW KQDSSLRAAL DLLPDALREA WQCDWSAVTE GLVEATNLFV LGRGLGLGAA QEAALKFKET CSLHAEAYSS AEVKHGPMAL VDRGFPVLAF AQPDETGAGT RAVVEEFTAR GAQVWMAGAG GNLPVAAAPH PLCAPLLTVQ SFYRAINALA LRRGFNPDLP PHLNKVTETV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGC TGTCAGACCC CACGTCCACC CTGATGTTCG CCGAGGCCGC TGAAGCGGCC GACGTGGTCG CGCGCCAGTT CTCGCGCAAC CACGCCACCA TGGAAACCCT GGCCGCCAGC CTGCGTGCTG CACCGCCGCC GTTCGTGGTC ACCTGTGCAC GCGGCAGCTC CGACCACGCC GCCACCTATG GCAAGTATCT GCTGGAAACC CAGCTGGGCC TGGTGGTCGC GTCGGCCTCG CCATCGGTGG GGTCGGTCTA CGCCGCACCA TTGCAGCTGC GCGGCGCACT GTTCATCGTC ATCTCGCAGT CGGGCAAGAG CCCGGACCTG CTGCGCAACG CCGAGGCCGC CAAGGCGGCC GGTGCGCGCG TGATCGCGCT GGTCAACGTC GAGGATTCAC CGCTGGCGCA GCTGGCCGAC ACGGTCATTC CGCTGCACGC GGGTGCCGAA AAGAGCGTGG CGGCGACCAA GAGTTACCTG GCGTCGCTGG CCGCGCTGCT GCAGCTGGCC GCGTACTGGA AGCAGGACAG CAGCCTGCGT GCGGCGCTGG ACCTGCTGCC CGATGCGCTG CGTGAGGCGT GGCAGTGCGA CTGGAGCGCG GTGACCGAAG GCCTGGTCGA GGCCACCAAC CTGTTCGTGC TCGGCCGTGG CCTTGGCCTG GGGGCGGCGC AGGAAGCGGC GCTGAAGTTC AAGGAAACCT GCAGCCTGCA TGCCGAAGCC TACAGCTCGG CTGAAGTGAA GCATGGCCCG ATGGCGCTGG TCGACCGCGG CTTCCCGGTG CTGGCCTTTG CCCAGCCGGA TGAAACCGGC GCCGGCACCC GTGCCGTCGT CGAAGAATTC ACCGCGCGTG GCGCACAGGT ATGGATGGCC GGTGCTGGCG GCAACCTGCC GGTGGCTGCT GCGCCGCACC CGCTGTGCGC ACCGCTGCTG ACCGTGCAGA GCTTCTACCG CGCGATCAAT GCGCTGGCGC TGCGTCGTGG CTTCAACCCG GACCTGCCGC CGCACCTGAA CAAGGTGACG GAGACCGTGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT