StmaA.17908.b

Transcriptional regulator, ArsR family / Methyltransferase fusion

CENTER ID: StmaA.17908.b
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17908.b.B1.GE43432 Full length(StmaA.17908.b) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17908.b.B1.PW38771 Full length(StmaA.17908.b) 1 309

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.2972

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLEDWSTRL KVFADATRVR LLALLEQEEL TVAELSAITR LAQPRVSTHL ARLKEAGLVR DRRAGVSAYY RFDEAQLDPA QRALWHALSN GSDDPLLRQD AERVAAVLAH RASDQNWADS VAGDMERHYS PGRTWEALAR TALPLLETGD VLDIASGDGV LAELVAPHAK RYICIDTSAR VVAAASERLR RLPNVEVREG DMHALPFKDN SFDLVVLMHA LTYASKPAQA VTEGARVLRP GGRLLLCSLA RHEHKAAVEA YGHVNLGFSD KELRRFVDKA GLQVSSLETV TREKRPPHFE VISLIAHKP
NT Sequence
ATGGATCTTG AAGACTGGTC GACCCGCCTG AAGGTGTTCG CCGATGCGAC CCGCGTGCGC CTGCTGGCGC TGCTGGAGCA GGAGGAACTG ACCGTGGCCG AACTGTCGGC GATCACCCGG CTGGCACAGC CACGCGTGTC CACCCACCTG GCGCGCCTGA AGGAAGCCGG CCTGGTCCGC GACCGCCGTG CCGGCGTATC GGCCTATTAC CGCTTCGACG AGGCCCAGCT GGATCCGGCG CAGCGTGCAT TGTGGCATGC CTTGAGCAAC GGAAGCGATG ACCCGCTGCT GCGCCAGGAC GCTGAACGCG TCGCTGCCGT GCTGGCCCAC CGCGCCTCCG ACCAGAACTG GGCCGACAGC GTGGCCGGCG ACATGGAACG CCACTACTCC CCGGGGCGCA CCTGGGAAGC CCTCGCCCGC ACCGCGCTGC CGCTGCTGGA GACCGGCGAC GTGCTGGACA TCGCCTCTGG TGACGGCGTG CTGGCCGAAC TGGTGGCCCC GCATGCCAAG CGCTACATCT GCATCGACAC CAGCGCGCGC GTGGTCGCCG CCGCCAGCGA GCGCCTGCGC CGTCTGCCCA ACGTGGAAGT GCGCGAGGGC GACATGCATG CGCTGCCCTT CAAGGACAAC AGTTTCGATC TGGTAGTGCT GATGCACGCG CTGACCTACG CCAGCAAGCC GGCGCAGGCG GTGACCGAAG GCGCGCGCGT GCTGCGCCCG GGCGGGCGCC TGCTGCTGTG CAGCCTGGCC CGCCACGAAC ACAAGGCCGC GGTGGAAGCC TACGGCCACG TCAACCTCGG CTTCAGCGAC AAGGAGCTGC GCAGGTTCGT CGACAAGGCC GGCCTGCAGG TATCCAGCCT GGAGACGGTC ACCCGCGAGA AGCGTCCGCC GCACTTCGAA GTGATTTCGC TGATCGCCCA CAAACCC
Details for StmaA.17908.b.B1.GE43432
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD LEDWSTRLKV FADATRVRLL ALLEQEELTV AELSAITRLA QPRVSTHLAR LKEAGLVRDR RAGVSAYYRF DEAQLDPAQR ALWHALSNGS DDPLLRQDAE RVAAVLAHRA SDQNWADSVA GDMERHYSPG RTWEALARTA LPLLETGDVL DIASGDGVLA ELVAPHAKRY ICIDTSARVV AAASERLRRL PNVEVREGDM HALPFKDNSF DLVVLMHALT YASKPAQAVT EGARVLRPGG RLLLCSLARH EHKAAVEAYG HVNLGFSDKE LRRFVDKAGL QVSSLETVTR EKRPPHFEVI SLIAHKP
Validated NT Sequence
atcttactca cttagggttt gtgggcgatc agcgaaatca cttcgaagtg cggcggacgc ttctcgcggg tgaccgtctc caggctggat acctgcaggc cggccttgtc gacgaacctg cgcagctcct tgtcgctgaa gccgaggttg acgtggccgt aggcttccac cgcggccttg tgttcgtggc gggccaggct gcacagcagc aggcgcccgc ccgggcgcag cacgcgcgcg ccttcggtca ccgcctgcgc cggcttgctg gcgtaggtca gcgcgtgcat cagcactacc agatcgaaac tgttgtcctt gaagggcagc gcatgcatgt cgccctcgcg cacttccacg ttgggcagac ggcgcaggcg ctcgctggcg gcggcgacca cgcgcgcgct ggtgtcgatg cagatgtagc gcttggcatg cggggccacc agttcggcca gcacgccgtc accagaggcg atgtccagca cgtcgccggt ctccagcagc ggcagcgcgg tgcgggcgag ggcttcccag gtgcgccccg gggagtagtg gcgttccatg tcgccggcca cgctgtcggc ccagttctgg tcggaggcgc ggtgggccag cacggcagcg acgcgttcag cgtcctggcg cagcagcggg tcatcgcttc cgttgctcaa ggcatgccac aatgcacgct gcgccggatc cagctgggcc tcgtcgaagc ggtaataggc cgatacgccg gcacggcggt cgcggaccag gccggcttcc ttcaggcgcg ccaggtgggt ggacacgcgt ggctgtgcca gccgggtgat cgccgacagt tcggccacgg tcagttcctc ctgctccagc agcgccagca ggcgcacgcg ggtcgcatcg gcgaacacct tcaggcgggt cgaccagtct tcaagatcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMD LEDWSTRLKV FADATRVRLL ALLEQEELTV AELSAITRLA QPRVSTHLAR LKEAGLVRDR RAGVSAYYRF DEAQLDPAQR ALWHALSNGS DDPLLRQDAE RVAAVLAHRA SDQNWADSVA GDMERHYSPG RTWEALARTA LPLLETGDVL DIASGDGVLA ELVAPHAKRY ICIDTSARVV AAASERLRRL PNVEVREGDM HALPFKDNSF DLVVLMHALT YASKPAQAVT EGARVLRPGG RLLLCSLARH EHKAAVEAYG HVNLGFSDKE LRRFVDKAGL QVSSLETVTR EKRPPHFEVI SLIAHKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc ttgaagactg gtcgacccgc ctgaaggtgt tcgccgatgc gacccgcgtg cgcctgctgg cgctgctgga gcaggaggaa ctgaccgtgg ccgaactgtc ggcgatcacc cggctggcac agccacgcgt gtccacccac ctggcgcgcc tgaaggaagc cggcctggtc cgcgaccgcc gtgccggcgt atcggcctat taccgcttcg acgaggccca gctggatccg gcgcagcgtg cattgtggca tgccttgagc aacggaagcg atgacccgct gctgcgccag gacgctgaac gcgtcgctgc cgtgctggcc caccgcgcct ccgaccagaa ctgggccgac agcgtggccg gcgacatgga acgccactac tccccggggc gcacctggga agccctcgcc cgcaccgcgc tgccgctgct ggagaccggc gacgtgctgg acatcgcctc tggtgacggc gtgctggccg aactggtggc cccgcatgcc aagcgctaca tctgcatcga caccagcgcg cgcgtggtcg ccgccgccag cgagcgcctg cgccgtctgc ccaacgtgga agtgcgcgag ggcgacatgc atgcgctgcc cttcaaggac aacagtttcg atctggtagt gctgatgcac gcgctgacct acgccagcaa gccggcgcag gcggtgaccg aaggcgcgcg cgtgctgcgc ccgggcgggc gcctgctgct gtgcagcctg gcccgccacg aacacaaggc cgcggtggaa gcctacggcc acgtcaacct cggcttcagc gacaaggagc tgcgcaggtt cgtcgacaag gccggcctgc aggtatccag cctggagacg gtcacccgcg agaagcgtcc gccgcacttc gaagtgattt cgctgatcgc ccacaaaccc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for StmaA.17908.b.B1.PW38771
PURIFICATION DATe: 12/10/2019
CONCENTRATION: 17.7mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD LEDWSTRLKV FADATRVRLL ALLEQEELTV AELSAITRLA QPRVSTHLAR LKEAGLVRDR RAGVSAYYRF DEAQLDPAQR ALWHALSNGS DDPLLRQDAE RVAAVLAHRA SDQNWADSVA GDMERHYSPG RTWEALARTA LPLLETGDVL DIASGDGVLA ELVAPHAKRY ICIDTSARVV AAASERLRRL PNVEVREGDM HALPFKDNSF DLVVLMHALT YASKPAQAVT EGARVLRPGG RLLLCSLARH EHKAAVEAYG HVNLGFSDKE LRRFVDKAGL QVSSLETVTR EKRPPHFEVI SLIAHKP
Validated NT Sequence
atcttactca cttagggttt gtgggcgatc agcgaaatca cttcgaagtg cggcggacgc ttctcgcggg tgaccgtctc caggctggat acctgcaggc cggccttgtc gacgaacctg cgcagctcct tgtcgctgaa gccgaggttg acgtggccgt aggcttccac cgcggccttg tgttcgtggc gggccaggct gcacagcagc aggcgcccgc ccgggcgcag cacgcgcgcg ccttcggtca ccgcctgcgc cggcttgctg gcgtaggtca gcgcgtgcat cagcactacc agatcgaaac tgttgtcctt gaagggcagc gcatgcatgt cgccctcgcg cacttccacg ttgggcagac ggcgcaggcg ctcgctggcg gcggcgacca cgcgcgcgct ggtgtcgatg cagatgtagc gcttggcatg cggggccacc agttcggcca gcacgccgtc accagaggcg atgtccagca cgtcgccggt ctccagcagc ggcagcgcgg tgcgggcgag ggcttcccag gtgcgccccg gggagtagtg gcgttccatg tcgccggcca cgctgtcggc ccagttctgg tcggaggcgc ggtgggccag cacggcagcg acgcgttcag cgtcctggcg cagcagcggg tcatcgcttc cgttgctcaa ggcatgccac aatgcacgct gcgccggatc cagctgggcc tcgtcgaagc ggtaataggc cgatacgccg gcacggcggt cgcggaccag gccggcttcc ttcaggcgcg ccaggtgggt ggacacgcgt ggctgtgcca gccgggtgat cgccgacagt tcggccacgg tcagttcctc ctgctccagc agcgccagca ggcgcacgcg ggtcgcatcg gcgaacacct tcaggcgggt cgaccagtct tcaagatcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMD LEDWSTRLKV FADATRVRLL ALLEQEELTV AELSAITRLA QPRVSTHLAR LKEAGLVRDR RAGVSAYYRF DEAQLDPAQR ALWHALSNGS DDPLLRQDAE RVAAVLAHRA SDQNWADSVA GDMERHYSPG RTWEALARTA LPLLETGDVL DIASGDGVLA ELVAPHAKRY ICIDTSARVV AAASERLRRL PNVEVREGDM HALPFKDNSF DLVVLMHALT YASKPAQAVT EGARVLRPGG RLLLCSLARH EHKAAVEAYG HVNLGFSDKE LRRFVDKAGL QVSSLETVTR EKRPPHFEVI SLIAHKP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC TTGAAGACTG GTCGACCCGC CTGAAGGTGT TCGCCGATGC GACCCGCGTG CGCCTGCTGG CGCTGCTGGA GCAGGAGGAA CTGACCGTGG CCGAACTGTC GGCGATCACC CGGCTGGCAC AGCCACGCGT GTCCACCCAC CTGGCGCGCC TGAAGGAAGC CGGCCTGGTC CGCGACCGCC GTGCCGGCGT ATCGGCCTAT TACCGCTTCG ACGAGGCCCA GCTGGATCCG GCGCAGCGTG CATTGTGGCA TGCCTTGAGC AACGGAAGCG ATGACCCGCT GCTGCGCCAG GACGCTGAAC GCGTCGCTGC CGTGCTGGCC CACCGCGCCT CCGACCAGAA CTGGGCCGAC AGCGTGGCCG GCGACATGGA ACGCCACTAC TCCCCGGGGC GCACCTGGGA AGCCCTCGCC CGCACCGCGC TGCCGCTGCT GGAGACCGGC GACGTGCTGG ACATCGCCTC TGGTGACGGC GTGCTGGCCG AACTGGTGGC CCCGCATGCC AAGCGCTACA TCTGCATCGA CACCAGCGCG CGCGTGGTCG CCGCCGCCAG CGAGCGCCTG CGCCGTCTGC CCAACGTGGA AGTGCGCGAG GGCGACATGC ATGCGCTGCC CTTCAAGGAC AACAGTTTCG ATCTGGTAGT GCTGATGCAC GCGCTGACCT ACGCCAGCAA GCCGGCGCAG GCGGTGACCG AAGGCGCGCG CGTGCTGCGC CCGGGCGGGC GCCTGCTGCT GTGCAGCCTG GCCCGCCACG AACACAAGGC CGCGGTGGAA GCCTACGGCC ACGTCAACCT CGGCTTCAGC GACAAGGAGC TGCGCAGGTT CGTCGACAAG GCCGGCCTGC AGGTATCCAG CCTGGAGACG GTCACCCGCG AGAAGCGTCC GCCGCACTTC GAAGTGATTT CGCTGATCGC CCACAAACCC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT