StmaA.17905.a
Thiamine-monophosphate kinase (EC 2.7.4.16)
| CENTER ID: | StmaA.17905.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.17905.a.B1.GE43429 | Full length(StmaA.17905.a) | 1 | 320 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6XEP
DEPOSITED: 6/12/2020
DETERMINATION: XRay
CLONE: StmaA.17905.a.B1.GE43429
PROTEIN: StmaA.17905.a.B1.PW38774
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.698 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLAEFALID RIRARTLERD DILLGIGDDA ALLQPRANEQ LVVTADTLNS GVHFPVETRA FDIGWKTLAV NLSDLAAMGA RPAWCTLALS LPEASEDWIE AFGDGFFALA DQHDIALIGG DTTRGPLSLS VTAMGQVGRG QALRRDRAQL GDDIWVSGTL GDAAGALRLW QQGALNLATA TLLADYESLR LRLLRPTPRV TLGLRLRAFA HAAVDVSDGL LADLGHIAAR SNVAAHVDAD SLPISHALRE LLGRDDARDC ALRGGDDYEL CFTAAADQRD ALHYLAESLD LPLTRIGRIA DGQGVHVDGE AADGGYQHFA
NT Sequence
ATGTCCCTGG CCGAATTCGC CCTCATAGAC CGTATCCGCG CCCGCACCCT CGAGCGCGAC GACATCCTGC TCGGCATCGG CGATGACGCT GCGCTGCTGC AGCCGCGGGC CAACGAACAG CTGGTGGTCA CCGCCGACAC GCTCAACAGC GGTGTGCATT TCCCGGTGGA AACCCGGGCC TTCGACATTG GCTGGAAGAC CTTGGCGGTC AACCTGTCGG ACCTCGCCGC GATGGGCGCG CGCCCGGCGT GGTGCACGCT GGCGCTGTCC CTGCCGGAAG CCTCTGAAGA CTGGATCGAG GCCTTCGGCG ATGGCTTCTT CGCCCTCGCC GACCAGCATG ACATCGCACT GATTGGTGGC GATACCACGC GTGGCCCGCT GTCGCTGTCG GTTACTGCGA TGGGACAGGT CGGCCGTGGC CAGGCATTGC GCCGCGACCG CGCGCAGTTG GGCGACGACA TCTGGGTCAG TGGCACGCTG GGCGATGCCG CCGGTGCGCT GCGCCTGTGG CAGCAGGGCG CGCTGAACCT GGCCACCGCC ACGTTGCTGG CCGACTACGA GAGCCTGCGT CTGCGCCTGC TGCGGCCGAC GCCGCGCGTC ACCCTCGGCC TGCGCCTGCG TGCGTTCGCG CATGCGGCGG TGGACGTGTC AGACGGCCTG CTGGCCGACC TGGGCCACAT CGCCGCACGC AGCAACGTAG CCGCGCATGT GGATGCCGAT TCGCTGCCGA TCTCGCACGC GCTGCGTGAG CTGCTCGGCC GCGACGATGC ACGCGACTGC GCACTGCGCG GTGGCGACGA CTACGAACTG TGCTTCACCG CCGCCGCCGA CCAGCGCGAC GCACTGCACT ACTTGGCCGA ATCGCTGGAC CTGCCGCTCA CCCGCATCGG CCGCATTGCC GATGGGCAAG GCGTGCACGT GGATGGCGAA GCCGCCGACG GCGGCTACCA GCACTTCGCG
Details for StmaA.17905.a.B1.GE43429
| HARVESTED ON: | 9/18/2019 |
| SEQUENCED ON: | 10/1/2019 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMS LAEFALIDRI RARTLERDDI LLGIGDDAAL LQPRANEQLV VTADTLNSGV HFPVETRAFD IGWKTLAVNL SDLAAMGARP AWCTLALSLP EASEDWIEAF GDGFFALADQ HDIALIGGDT TRGPLSLSVT AMGQVGRGQA LRRDRAQLGD DIWVSGTLGD AAGALRLWQQ GALNLATATL LADYESLRLR LLRPTPRVTL GLRLRAFAHA AVDVSDGLLA DLGHIAARSN VAAHVDADSL PISHALRELL GRDDARDCAL RGGDDYELCF TAAADQRDAL HYLAESLDLP LTRIGRIADG QGVHVDG
Validated NT Sequence
ttcgccatcc acgtgcacgc cttgcccatc ggcaatgcgg ccgatgcggg tgagcggcag gtccagcgat tcggccaagt agtgcagtgc gtcgcgctgg tcggcggcgg cggtgaagca cagttcgtag tcgtcgccac cgcgcagtgc gcagtcgcgt gcatcgtcgc ggccgagcag ctcacgcagc gcgtgcgaga tcggcagcga atcggcatcc acatgcgcgg ctacgttgct gcgtgcggcg atgtggccca ggtcggccag caggccgtct gacacgtcca ccgccgcatg cgcgaacgca cgcaggcgca ggccgagggt gacgcgcggc gtcggccgca gcaggcgcag acgcaggctc tcgtagtcgg ccagcaacgt ggcggtggcc aggttcagcg cgccctgctg ccacaggcgc agcgcaccgg cggcatcgcc cagcgtgcca ctgacccaga tgtcgtcgcc caactgcgcg cggtcgcggc gcaatgcctg gccacggccg acctgtccca tcgcagtaac cgacagcgac agcgggccac gcgtggtatc gccaccaatc agtgcgatgt catgctggtc ggcgagggcg aagaagccat cgccgaaggc ctcgatccag tcttcagagg cttccggcag ggacagcgcc agcgtgcacc acgccgggcg cgcgcccatc gcggcgaggt ccgacaggtt gaccgccaag gtcttccagc caatgtcgaa ggcccgggtt tccaccggga aatgcacacc gctgttgagc gtgtcggcgg tgaccaccag ctgttcgttg gcccgcggct gcagcagcgc agcgtcatcg ccgatgccga gcaggatgtc gtcgcgctcg agggtgcggg cgcggatacg gtctatgagg gcgaattcgg ccagggacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS LAEFALIDRI RARTLERDDI LLGIGDDAAL LQPRANEQLV VTADTLNSGV HFPVETRAFD IGWKTLAVNL SDLAAMGARP AWCTLALSLP EASEDWIEAF GDGFFALADQ HDIALIGGDT TRGPLSLSVT AMGQVGRGQA LRRDRAQLGD DIWVSGTLGD AAGALRLWQQ GALNLATATL LADYESLRLR LLRPTPRVTL GLRLRAFAHA AVDVSDGLLA DLGHIAARSN VAAHVDADSL PISHALRELL GRDDARDCAL RGGDDYELCF TAAADQRDAL HYLAESLDLP LTRIGRIADG QGVHVDGEAA DGGYQHFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccc tggccgaatt cgccctcata gaccgtatcc gcgcccgcac cctcgagcgc gacgacatcc tgctcggcat cggcgatgac gctgcgctgc tgcagccgcg ggccaacgaa cagctggtgg tcaccgccga cacgctcaac agcggtgtgc atttcccggt ggaaacccgg gccttcgaca ttggctggaa gaccttggcg gtcaacctgt cggacctcgc cgcgatgggc gcgcgcccgg cgtggtgcac gctggcgctg tccctgccgg aagcctctga agactggatc gaggccttcg gcgatggctt cttcgccctc gccgaccagc atgacatcgc actgattggt ggcgatacca cgcgtggccc gctgtcgctg tcggttactg cgatgggaca ggtcggccgt ggccaggcat tgcgccgcga ccgcgcgcag ttgggcgacg acatctgggt cagtggcacg ctgggcgatg ccgccggtgc gctgcgcctg tggcagcagg gcgcgctgaa cctggccacc gccacgttgc tggccgacta cgagagcctg cgtctgcgcc tgctgcggcc gacgccgcgc gtcaccctcg gcctgcgcct gcgtgcgttc gcgcatgcgg cggtggacgt gtcagacggc ctgctggccg acctgggcca catcgccgca cgcagcaacg tagccgcgca tgtggatgcc gattcgctgc cgatctcgca cgcgctgcgt gagctgctcg gccgcgacga tgcacgcgac tgcgcactgc gcggtggcga cgactacgaa ctgtgcttca ccgccgccgc cgaccagcgc gacgcactgc actacttggc cgaatcgctg gacctgccgc tcacccgcat cggccgcatt gccgatgggc aaggcgtgca cgtggatggc gaagccgccg acggcggcta ccagcacttc gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat