StmaA.17901.a

Peptide chain release factor 1 (prfA)

CENTER ID: StmaA.17901.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17901.a.B1.GE43428 Full length(StmaA.17901.a) 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17901.a.B1.PW38770 Full length(StmaA.17901.a) 1 360

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.842

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPTLRRKLE ALAERREELE RLLAEPDVVA DNTRFRDLSR EFAQLEPIAI ALADESRAKA DLAAAEGMRA DPDLRELADE EIAAAQARLL ELEQELALLL VPRDPRDEGN LFLEVRAGTG GDEAAIFAGD LFRMYARYAE RQGWKVEIES DNPGEHGGYK EVVARVVGRG AFSRLKFESG THRVQRVPAT ESQGRIHTSA ATVAIIPEAD EVDDIVINPA DLKVDTFRSS GAGGQHVNKT ESAIRITHVP TGVVVECQTE RSQHANRDKA MKRLKAQLLD AERQRQDAAQ AESRRLQVGS GDRSQRIRTY NFPQGRITDH RVEGLTLYDL PNILAGDLDP LLQRLSHEHQ VDALAQLSAG
NT Sequence
ATGACGCCGA CCCTGCGCCG TAAGCTGGAA GCGCTGGCCG AGCGCCGCGA AGAACTGGAA CGCCTGCTCG CCGAACCCGA CGTGGTCGCC GACAACACCC GTTTCCGCGA CCTTTCGCGC GAATTCGCCC AACTTGAACC GATCGCCATC GCCTTGGCCG ATGAATCCCG TGCCAAGGCT GATCTGGCCG CCGCCGAAGG CATGCGCGCC GATCCCGACC TGCGCGAACT GGCCGACGAG GAAATCGCCG CCGCGCAGGC GCGCCTGCTG GAACTGGAGC AGGAACTGGC GCTGCTGCTG GTACCGCGCG ACCCGCGCGA CGAAGGCAAC CTGTTCCTGG AAGTGCGCGC GGGTACCGGC GGCGATGAAG CGGCGATCTT TGCCGGCGAC CTGTTCCGCA TGTACGCCCG CTACGCCGAG CGCCAGGGCT GGAAGGTCGA GATCGAATCG GACAATCCCG GCGAGCACGG TGGCTACAAG GAAGTGGTGG CACGGGTGGT CGGCCGTGGT GCGTTCTCGC GCCTGAAGTT CGAATCGGGC ACGCACCGTG TGCAGCGCGT GCCGGCCACC GAATCGCAGG GCCGCATCCA TACCTCGGCG GCCACCGTGG CGATCATCCC GGAGGCCGAC GAAGTCGATG ACATCGTCAT CAACCCGGCC GACCTGAAGG TGGATACGTT CCGCTCCTCC GGCGCCGGTG GCCAGCACGT CAACAAGACC GAGTCGGCGA TCCGCATCAC CCACGTACCG ACCGGCGTGG TGGTGGAATG CCAGACCGAA CGCAGCCAGC ACGCCAACCG CGACAAGGCG ATGAAGCGCC TGAAAGCGCA GCTGCTCGAC GCCGAGCGCC AACGCCAGGA CGCGGCCCAG GCCGAGTCGA GGCGCCTGCA GGTCGGCAGT GGTGACCGCA GCCAGCGCAT CCGCACCTAC AACTTCCCGC AGGGGCGCAT CACCGACCAC CGCGTGGAGG GCCTGACCCT GTACGACCTG CCCAACATCC TGGCCGGTGA CCTCGATCCG CTGCTGCAGC GGCTCAGCCA CGAACACCAG GTCGACGCCC TGGCCCAGCT GTCGGCGGGC
Details for StmaA.17901.a.B1.GE43428
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT PTLRRKLEAL AERREELERL LAEPDVVADN TRFRDLSREF AQLEPIAIAL ADESRAKADL AAAEGMRADP DLRELADEEI AAAQARLLEL EQELALLLVP RDPRDEGNLF LEVRAGTGGD EAAIFAGDLF RMYARYAERQ GWKVEIESDN PGEHGGYKEV VARVVGRGAF SRLKFESGTH RVQRVPATES QGRIHTSAAT VAIIPEADEV DDIVINPADL KVDTFRSSGA GGQHVNKTES AIRITHVPTG VVVECQTERS QHANRDKAMK RLKAQLLDAE RQRQDAAQAE SRRLQVGSGD RSQRIRTYNF PQGRITDHRV EGLTLYDLPN ILAGDLDPLL QRLSHEHQXX
Validated NT Sequence
ggngtnnncc tggtgttcgt ggctgagccg ctgcagcagc ggatcgaggt caccggccag gatgttgggc aggtcgtaca gggtcaggcc ctccacgcgg tggtcggtga tgcgcccctg cgggaagttg taggtgcgga tgcgctggct gcggtcacca ctgccgacct gcaggcgcct cgactcggcc tgggccgcgt cctggcgttg gcgctcggcg tcgagcagct gcgctttcag gcgcttcatc gccttgtcgc ggttggcgtg ctggctgcgt tcggtctggc attccaccac cacgccggtc ggtacgtggg tgatgcggat cgccgactcg gtcttgttga cgtgctggcc accggcgccg gaggagcgga acgtatccac cttcaggtcg gccgggttga tgacgatgtc atcgacttcg tcggcctccg ggatgatcgc cacggtggcc gccgaggtat ggatgcggcc ctgcgattcg gtggccggca cgcgctgcac acggtgcgtg cccgattcga acttcaggcg cgagaacgca ccacggccga ccacccgtgc caccacttcc ttgtagccac cgtgctcgcc gggattgtcc gattcgatct cgaccttcca gccctggcgc tcggcgtagc gggcgtacat gcggaacagg tcgccggcaa agatcgccgc ttcatcgccg ccggtacccg cgcgcacttc caggaacagg ttgccttcgt cgcgcgggtc gcgcggtacc agcagcagcg ccagttcctg ctccagttcc agcaggcgcg cctgcgcggc ggcgatttcc tcgtcggcca gttcgcgcag gtcgggatcg gcgcgcatgc cttcggcggc ggccagatca gccttggcac gggattcatc ggccaaggcg atggcgatcg gttcaagttg ggcgaattcg cgcgaaaggt cgcggaaacg ggtgttgtcg gcgaccacgt cgggttcggc gagcaggcgt tccagttctt cgcggcgctc ggccagcgct tccagcttac ggcgcaaggt cggcgtcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT PTLRRKLEAL AERREELERL LAEPDVVADN TRFRDLSREF AQLEPIAIAL ADESRAKADL AAAEGMRADP DLRELADEEI AAAQARLLEL EQELALLLVP RDPRDEGNLF LEVRAGTGGD EAAIFAGDLF RMYARYAERQ GWKVEIESDN PGEHGGYKEV VARVVGRGAF SRLKFESGTH RVQRVPATES QGRIHTSAAT VAIIPEADEV DDIVINPADL KVDTFRSSGA GGQHVNKTES AIRITHVPTG VVVECQTERS QHANRDKAMK RLKAQLLDAE RQRQDAAQAE SRRLQVGSGD RSQRIRTYNF PQGRITDHRV EGLTLYDLPN ILAGDLDPLL QRLSHEHQVD ALAQLSAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgc cgaccctgcg ccgtaagctg gaagcgctgg ccgagcgccg cgaagaactg gaacgcctgc tcgccgaacc cgacgtggtc gccgacaaca cccgtttccg cgacctttcg cgcgaattcg cccaacttga accgatcgcc atcgccttgg ccgatgaatc ccgtgccaag gctgatctgg ccgccgccga aggcatgcgc gccgatcccg acctgcgcga actggccgac gaggaaatcg ccgccgcgca ggcgcgcctg ctggaactgg agcaggaact ggcgctgctg ctggtaccgc gcgacccgcg cgacgaaggc aacctgttcc tggaagtgcg cgcgggtacc ggcggcgatg aagcggcgat ctttgccggc gacctgttcc gcatgtacgc ccgctacgcc gagcgccagg gctggaaggt cgagatcgaa tcggacaatc ccggcgagca cggtggctac aaggaagtgg tggcacgggt ggtcggccgt ggtgcgttct cgcgcctgaa gttcgaatcg ggcacgcacc gtgtgcagcg cgtgccggcc accgaatcgc agggccgcat ccatacctcg gcggccaccg tggcgatcat cccggaggcc gacgaagtcg atgacatcgt catcaacccg gccgacctga aggtggatac gttccgctcc tccggcgccg gtggccagca cgtcaacaag accgagtcgg cgatccgcat cacccacgta ccgaccggcg tggtggtgga atgccagacc gaacgcagcc agcacgccaa ccgcgacaag gcgatgaagc gcctgaaagc gcagctgctc gacgccgagc gccaacgcca ggacgcggcc caggccgagt cgaggcgcct gcaggtcggc agtggtgacc gcagccagcg catccgcacc tacaacttcc cgcaggggcg catcaccgac caccgcgtgg agggcctgac cctgtacgac ctgcccaaca tcctggccgg tgacctcgat ccgctgctgc agcggctcag ccacgaacac caggtcgacg ccctggccca gctgtcggcg ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for StmaA.17901.a.B1.PW38770
PURIFICATION DATe: 12/10/2019
CONCENTRATION: 44mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT PTLRRKLEAL AERREELERL LAEPDVVADN TRFRDLSREF AQLEPIAIAL ADESRAKADL AAAEGMRADP DLRELADEEI AAAQARLLEL EQELALLLVP RDPRDEGNLF LEVRAGTGGD EAAIFAGDLF RMYARYAERQ GWKVEIESDN PGEHGGYKEV VARVVGRGAF SRLKFESGTH RVQRVPATES QGRIHTSAAT VAIIPEADEV DDIVINPADL KVDTFRSSGA GGQHVNKTES AIRITHVPTG VVVECQTERS QHANRDKAMK RLKAQLLDAE RQRQDAAQAE SRRLQVGSGD RSQRIRTYNF PQGRITDHRV EGLTLYDLPN ILAGDLDPLL QRLSHEHQXX
Validated NT Sequence
ggngtnnncc tggtgttcgt ggctgagccg ctgcagcagc ggatcgaggt caccggccag gatgttgggc aggtcgtaca gggtcaggcc ctccacgcgg tggtcggtga tgcgcccctg cgggaagttg taggtgcgga tgcgctggct gcggtcacca ctgccgacct gcaggcgcct cgactcggcc tgggccgcgt cctggcgttg gcgctcggcg tcgagcagct gcgctttcag gcgcttcatc gccttgtcgc ggttggcgtg ctggctgcgt tcggtctggc attccaccac cacgccggtc ggtacgtggg tgatgcggat cgccgactcg gtcttgttga cgtgctggcc accggcgccg gaggagcgga acgtatccac cttcaggtcg gccgggttga tgacgatgtc atcgacttcg tcggcctccg ggatgatcgc cacggtggcc gccgaggtat ggatgcggcc ctgcgattcg gtggccggca cgcgctgcac acggtgcgtg cccgattcga acttcaggcg cgagaacgca ccacggccga ccacccgtgc caccacttcc ttgtagccac cgtgctcgcc gggattgtcc gattcgatct cgaccttcca gccctggcgc tcggcgtagc gggcgtacat gcggaacagg tcgccggcaa agatcgccgc ttcatcgccg ccggtacccg cgcgcacttc caggaacagg ttgccttcgt cgcgcgggtc gcgcggtacc agcagcagcg ccagttcctg ctccagttcc agcaggcgcg cctgcgcggc ggcgatttcc tcgtcggcca gttcgcgcag gtcgggatcg gcgcgcatgc cttcggcggc ggccagatca gccttggcac gggattcatc ggccaaggcg atggcgatcg gttcaagttg ggcgaattcg cgcgaaaggt cgcggaaacg ggtgttgtcg gcgaccacgt cgggttcggc gagcaggcgt tccagttctt cgcggcgctc ggccagcgct tccagcttac ggcgcaaggt cggcgtcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMT PTLRRKLEAL AERREELERL LAEPDVVADN TRFRDLSREF AQLEPIAIAL ADESRAKADL AAAEGMRADP DLRELADEEI AAAQARLLEL EQELALLLVP RDPRDEGNLF LEVRAGTGGD EAAIFAGDLF RMYARYAERQ GWKVEIESDN PGEHGGYKEV VARVVGRGAF SRLKFESGTH RVQRVPATES QGRIHTSAAT VAIIPEADEV DDIVINPADL KVDTFRSSGA GGQHVNKTES AIRITHVPTG VVVECQTERS QHANRDKAMK RLKAQLLDAE RQRQDAAQAE SRRLQVGSGD RSQRIRTYNF PQGRITDHRV EGLTLYDLPN ILAGDLDPLL QRLSHEHQVD ALAQLSAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGC CGACCCTGCG CCGTAAGCTG GAAGCGCTGG CCGAGCGCCG CGAAGAACTG GAACGCCTGC TCGCCGAACC CGACGTGGTC GCCGACAACA CCCGTTTCCG CGACCTTTCG CGCGAATTCG CCCAACTTGA ACCGATCGCC ATCGCCTTGG CCGATGAATC CCGTGCCAAG GCTGATCTGG CCGCCGCCGA AGGCATGCGC GCCGATCCCG ACCTGCGCGA ACTGGCCGAC GAGGAAATCG CCGCCGCGCA GGCGCGCCTG CTGGAACTGG AGCAGGAACT GGCGCTGCTG CTGGTACCGC GCGACCCGCG CGACGAAGGC AACCTGTTCC TGGAAGTGCG CGCGGGTACC GGCGGCGATG AAGCGGCGAT CTTTGCCGGC GACCTGTTCC GCATGTACGC CCGCTACGCC GAGCGCCAGG GCTGGAAGGT CGAGATCGAA TCGGACAATC CCGGCGAGCA CGGTGGCTAC AAGGAAGTGG TGGCACGGGT GGTCGGCCGT GGTGCGTTCT CGCGCCTGAA GTTCGAATCG GGCACGCACC GTGTGCAGCG CGTGCCGGCC ACCGAATCGC AGGGCCGCAT CCATACCTCG GCGGCCACCG TGGCGATCAT CCCGGAGGCC GACGAAGTCG ATGACATCGT CATCAACCCG GCCGACCTGA AGGTGGATAC GTTCCGCTCC TCCGGCGCCG GTGGCCAGCA CGTCAACAAG ACCGAGTCGG CGATCCGCAT CACCCACGTA CCGACCGGCG TGGTGGTGGA ATGCCAGACC GAACGCAGCC AGCACGCCAA CCGCGACAAG GCGATGAAGC GCCTGAAAGC GCAGCTGCTC GACGCCGAGC GCCAACGCCA GGACGCGGCC CAGGCCGAGT CGAGGCGCCT GCAGGTCGGC AGTGGTGACC GCAGCCAGCG CATCCGCACC TACAACTTCC CGCAGGGGCG CATCACCGAC CACCGCGTGG AGGGCCTGAC CCTGTACGAC CTGCCCAACA TCCTGGCCGG TGACCTCGAT CCGCTGCTGC AGCGGCTCAG CCACGAACAC CAGGTCGACG CCCTGGCCCA GCTGTCGGCG GGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT