StmaA.17885.a

Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) (asd)

CENTER ID: StmaA.17885.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17885.a.B1.GE43424 Full length(StmaA.17885.a) 1 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17885.a.B1.PW38780 Full length(StmaA.17885.a) 1 342

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3213

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNAQRSFHV AVVGATGAVG ETMLSILAER GFPVGTLSVL ASERSAGGEI EFNGQKVTVQ DLATFDPSGV EIALFSAGGS VSKEYAPKFA AAGAVVIDNS SAFRYDDDVP LVVSEVNPEQ VANRPRGIIA NPNCSTMQML VALAPLHRKY GIERINVSTY QSVSGGGRSA TEELGKQTGQ LLSFQEIDPQ RFPVQIAFNL IPHIDDFQDN GFTKEEMKLI WETRKILGDD SILVNPTAVR VPVFYGHSES VAIETRDKVT VAEARALLEQ SPGIEVVDRH EAGGYPTPVT HASGTDAVYV GRIREDLSHP RGLNLWIVSD NVRKGAALNA VQLAELVAAE KR
NT Sequence
ATGAGCAATG CACAGCGCAG TTTCCACGTC GCCGTCGTCG GTGCCACCGG TGCGGTCGGC GAGACCATGT TGTCGATCCT GGCCGAGCGT GGTTTCCCGG TCGGTACCCT GAGCGTCCTG GCTTCCGAGC GTTCGGCCGG CGGCGAGATC GAATTCAACG GCCAGAAGGT CACGGTCCAG GACCTGGCCA CCTTCGATCC GAGCGGCGTC GAGATCGCGC TGTTCTCGGC CGGTGGCAGC GTGTCCAAGG AATACGCACC GAAGTTCGCC GCTGCCGGTG CGGTGGTGAT CGACAACTCT TCGGCCTTCC GTTACGACGA TGACGTGCCG CTGGTGGTGT CCGAGGTCAA TCCGGAACAG GTGGCCAACC GTCCGCGCGG CATCATCGCC AACCCGAACT GCTCGACCAT GCAGATGCTG GTGGCGCTGG CGCCACTGCA CCGCAAGTAC GGCATCGAGC GCATCAACGT CTCCACCTAC CAGTCGGTGT CCGGCGGCGG TCGTTCGGCT ACCGAGGAAC TGGGCAAGCA GACCGGCCAG CTGCTGAGCT TCCAGGAAAT CGATCCGCAG CGCTTCCCGG TGCAGATTGC TTTCAACCTG ATCCCGCACA TCGACGACTT CCAGGACAAT GGCTTCACCA AGGAAGAAAT GAAGCTGATC TGGGAAACCC GCAAGATCCT CGGCGACGAC AGCATCCTGG TGAACCCGAC TGCGGTGCGC GTGCCGGTGT TCTACGGCCA CTCCGAGTCG GTGGCGATCG AGACCCGCGA CAAGGTCACC GTCGCCGAAG CGCGCGCGCT GCTGGAGCAG TCGCCGGGCA TCGAAGTGGT GGATCGTCAC GAAGCCGGTG GTTACCCAAC CCCGGTCACC CACGCCTCGG GCACGGACGC GGTGTACGTC GGCCGCATCC GCGAGGACCT CTCGCACCCG CGCGGCCTGA ACCTGTGGAT CGTGTCGGAC AACGTGCGCA AGGGCGCGGC GTTGAATGCC GTGCAGCTGG CCGAGCTGGT CGCCGCCGAG AAGCGC
Details for StmaA.17885.a.B1.GE43424
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMS NAQRSFHVAV VGATGAVGET MLSILAERGF PVGTLSVLAS ERSAGGEIEF NGQKVTVQDL ATFDPSGVEI ALFSAGGSVS KEYAPKFAAA GAVVIDNSSA FRYDDDVPLV VSEVNPEQVA NRPRGIIANP NCSTMQMLVA LAPLHRKYGI ERINVSTYQS VSGGGRSATE ELGKQTGQLL SFQEIDPQRF PVQIAFNLIP HIDDFQDNGF TKEEMKLIWE TRKILGDDSI LVNPTAVRVP VFYGHSESVA IETRDKVTVA EARALLEQSP GIEVVDRHEA GGYPTPVTHA SGTDAVYVGR IREDLSHPRG LNLWIVSDNV RKGAALNAV
Validated NT Sequence
tgcacggcat tcaacgccgc gcccttgcgc acgttgtccg acacgatcca caggttcagg ccgcgcgggt gcgagaggtc ctcgcggatg cggccgacgt acaccgcgtc cgtgcccgag gcgtgggtga ccggggttgg gtaaccaccg gcttcgtgac gatccaccac ttcgatgccc ggcgactgct ccagcagcgc gcgcgcttcg gcgacggtga ccttgtcgcg ggtctcgatc gccaccgact cggagtggcc gtagaacacc ggcacgcgca ccgcagtcgg gttcaccagg atgctgtcgt cgccgaggat cttgcgggtt tcccagatca gcttcatttc ttccttggtg aagccattgt cctggaagtc gtcgatgtgc gggatcaggt tgaaagcaat ctgcaccggg aagcgctgcg gatcgatttc ctggaagctc agcagctggc cggtctgctt gcccagttcc tcggtagccg aacgaccgcc gccggacacc gactggtagg tggagacgtt gatgcgctcg atgccgtact tgcggtgcag tggcgccagc gccaccagca tctgcatggt cgagcagttc gggttggcga tgatgccgcg cggacggttg gccacctgtt ccggattgac ctcggacacc accagcggca cgtcatcgtc gtaacggaag gccgaagagt tgtcgatcac caccgcaccg gcagcggcga acttcggtgc gtattccttg gacacgctgc caccggccga gaacagcgcg atctcgacgc cgctcggatc gaaggtggcc aggtcctgga ccgtgacctt ctggccgttg aattcgatct cgccgccggc cgaacgctcg gaagccagga cgctcagggt accgaccggg aaaccacgct cggccaggat cgacaacatg gtctcgccga ccgcaccggt ggcaccgacg acggcgacgt ggaaactgcg ctgtgcattg ctcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMS NAQRSFHVAV VGATGAVGET MLSILAERGF PVGTLSVLAS ERSAGGEIEF NGQKVTVQDL ATFDPSGVEI ALFSAGGSVS KEYAPKFAAA GAVVIDNSSA FRYDDDVPLV VSEVNPEQVA NRPRGIIANP NCSTMQMLVA LAPLHRKYGI ERINVSTYQS VSGGGRSATE ELGKQTGQLL SFQEIDPQRF PVQIAFNLIP HIDDFQDNGF TKEEMKLIWE TRKILGDDSI LVNPTAVRVP VFYGHSESVA IETRDKVTVA EARALLEQSP GIEVVDRHEA GGYPTPVTHA SGTDAVYVGR IREDLSHPRG LNLWIVSDNV RKGAALNAVQ LAELVAAEKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca atgcacagcg cagtttccac gtcgccgtcg tcggtgccac cggtgcggtc ggcgagacca tgttgtcgat cctggccgag cgtggtttcc cggtcggtac cctgagcgtc ctggcttccg agcgttcggc cggcggcgag atcgaattca acggccagaa ggtcacggtc caggacctgg ccaccttcga tccgagcggc gtcgagatcg cgctgttctc ggccggtggc agcgtgtcca aggaatacgc accgaagttc gccgctgccg gtgcggtggt gatcgacaac tcttcggcct tccgttacga cgatgacgtg ccgctggtgg tgtccgaggt caatccggaa caggtggcca accgtccgcg cggcatcatc gccaacccga actgctcgac catgcagatg ctggtggcgc tggcgccact gcaccgcaag tacggcatcg agcgcatcaa cgtctccacc taccagtcgg tgtccggcgg cggtcgttcg gctaccgagg aactgggcaa gcagaccggc cagctgctga gcttccagga aatcgatccg cagcgcttcc cggtgcagat tgctttcaac ctgatcccgc acatcgacga cttccaggac aatggcttca ccaaggaaga aatgaagctg atctgggaaa cccgcaagat cctcggcgac gacagcatcc tggtgaaccc gactgcggtg cgcgtgccgg tgttctacgg ccactccgag tcggtggcga tcgagacccg cgacaaggtc accgtcgccg aagcgcgcgc gctgctggag cagtcgccgg gcatcgaagt ggtggatcgt cacgaagccg gtggttaccc aaccccggtc acccacgcct cgggcacgga cgcggtgtac gtcggccgca tccgcgagga cctctcgcac ccgcgcggcc tgaacctgtg gatcgtgtcg gacaacgtgc gcaagggcgc ggcgttgaat gccgtgcagc tggccgagct ggtcgccgcc gagaagcgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for StmaA.17885.a.B1.PW38780
PURIFICATION DATe: 12/19/2019
CONCENTRATION: 36mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NAQRSFHVAV VGATGAVGET MLSILAERGF PVGTLSVLAS ERSAGGEIEF NGQKVTVQDL ATFDPSGVEI ALFSAGGSVS KEYAPKFAAA GAVVIDNSSA FRYDDDVPLV VSEVNPEQVA NRPRGIIANP NCSTMQMLVA LAPLHRKYGI ERINVSTYQS VSGGGRSATE ELGKQTGQLL SFQEIDPQRF PVQIAFNLIP HIDDFQDNGF TKEEMKLIWE TRKILGDDSI LVNPTAVRVP VFYGHSESVA IETRDKVTVA EARALLEQSP GIEVVDRHEA GGYPTPVTHA SGTDAVYVGR IREDLSHPRG LNLWIVSDNV RKGAALNAV
Validated NT Sequence
tgcacggcat tcaacgccgc gcccttgcgc acgttgtccg acacgatcca caggttcagg ccgcgcgggt gcgagaggtc ctcgcggatg cggccgacgt acaccgcgtc cgtgcccgag gcgtgggtga ccggggttgg gtaaccaccg gcttcgtgac gatccaccac ttcgatgccc ggcgactgct ccagcagcgc gcgcgcttcg gcgacggtga ccttgtcgcg ggtctcgatc gccaccgact cggagtggcc gtagaacacc ggcacgcgca ccgcagtcgg gttcaccagg atgctgtcgt cgccgaggat cttgcgggtt tcccagatca gcttcatttc ttccttggtg aagccattgt cctggaagtc gtcgatgtgc gggatcaggt tgaaagcaat ctgcaccggg aagcgctgcg gatcgatttc ctggaagctc agcagctggc cggtctgctt gcccagttcc tcggtagccg aacgaccgcc gccggacacc gactggtagg tggagacgtt gatgcgctcg atgccgtact tgcggtgcag tggcgccagc gccaccagca tctgcatggt cgagcagttc gggttggcga tgatgccgcg cggacggttg gccacctgtt ccggattgac ctcggacacc accagcggca cgtcatcgtc gtaacggaag gccgaagagt tgtcgatcac caccgcaccg gcagcggcga acttcggtgc gtattccttg gacacgctgc caccggccga gaacagcgcg atctcgacgc cgctcggatc gaaggtggcc aggtcctgga ccgtgacctt ctggccgttg aattcgatct cgccgccggc cgaacgctcg gaagccagga cgctcagggt accgaccggg aaaccacgct cggccaggat cgacaacatg gtctcgccga ccgcaccggt ggcaccgacg acggcgacgt ggaaactgcg ctgtgcattg ctcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMS NAQRSFHVAV VGATGAVGET MLSILAERGF PVGTLSVLAS ERSAGGEIEF NGQKVTVQDL ATFDPSGVEI ALFSAGGSVS KEYAPKFAAA GAVVIDNSSA FRYDDDVPLV VSEVNPEQVA NRPRGIIANP NCSTMQMLVA LAPLHRKYGI ERINVSTYQS VSGGGRSATE ELGKQTGQLL SFQEIDPQRF PVQIAFNLIP HIDDFQDNGF TKEEMKLIWE TRKILGDDSI LVNPTAVRVP VFYGHSESVA IETRDKVTVA EARALLEQSP GIEVVDRHEA GGYPTPVTHA SGTDAVYVGR IREDLSHPRG LNLWIVSDNV RKGAALNAVQ LAELVAAEKR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA ATGCACAGCG CAGTTTCCAC GTCGCCGTCG TCGGTGCCAC CGGTGCGGTC GGCGAGACCA TGTTGTCGAT CCTGGCCGAG CGTGGTTTCC CGGTCGGTAC CCTGAGCGTC CTGGCTTCCG AGCGTTCGGC CGGCGGCGAG ATCGAATTCA ACGGCCAGAA GGTCACGGTC CAGGACCTGG CCACCTTCGA TCCGAGCGGC GTCGAGATCG CGCTGTTCTC GGCCGGTGGC AGCGTGTCCA AGGAATACGC ACCGAAGTTC GCCGCTGCCG GTGCGGTGGT GATCGACAAC TCTTCGGCCT TCCGTTACGA CGATGACGTG CCGCTGGTGG TGTCCGAGGT CAATCCGGAA CAGGTGGCCA ACCGTCCGCG CGGCATCATC GCCAACCCGA ACTGCTCGAC CATGCAGATG CTGGTGGCGC TGGCGCCACT GCACCGCAAG TACGGCATCG AGCGCATCAA CGTCTCCACC TACCAGTCGG TGTCCGGCGG CGGTCGTTCG GCTACCGAGG AACTGGGCAA GCAGACCGGC CAGCTGCTGA GCTTCCAGGA AATCGATCCG CAGCGCTTCC CGGTGCAGAT TGCTTTCAAC CTGATCCCGC ACATCGACGA CTTCCAGGAC AATGGCTTCA CCAAGGAAGA AATGAAGCTG ATCTGGGAAA CCCGCAAGAT CCTCGGCGAC GACAGCATCC TGGTGAACCC GACTGCGGTG CGCGTGCCGG TGTTCTACGG CCACTCCGAG TCGGTGGCGA TCGAGACCCG CGACAAGGTC ACCGTCGCCG AAGCGCGCGC GCTGCTGGAG CAGTCGCCGG GCATCGAAGT GGTGGATCGT CACGAAGCCG GTGGTTACCC AACCCCGGTC ACCCACGCCT CGGGCACGGA CGCGGTGTAC GTCGGCCGCA TCCGCGAGGA CCTCTCGCAC CCGCGCGGCC TGAACCTGTG GATCGTGTCG GACAACGTGC GCAAGGGCGC GGCGTTGAAT GCCGTGCAGC TGGCCGAGCT GGTCGCCGCC GAGAAGCGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT