SSGCID: Target Page

StmaA.17867.a

Electron transfer flavoprotein, alpha subunit (etfA)

CENTER ID:	StmaA.17867.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.17867.a.B1.GE43419	Full length(StmaA.17867.a)		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.614

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKILVIAEH HDGKLNAATA KTVSAAAAIS GASIDVLVLA ADPAAVAAEA AKIAGVAKVL TVANAANAQA IAQVLAPQIA QLAKGYTHVF GPSTTFGKDL MPCVAALLGV NQVSDLMTVE GSHTFKRPIY AGNAIITVEA PADQIVVATV RAASWPEAAQ GGSAAIEAAS VDAALPTHTR FVGLAAGASD RPDLQSAKRV VSGGRGVGSE ENFKVIFQLA DKLGAAVGAS RAAVDAGYVP SDLQVGQTGK IIAPELYVAV GISGAIQHLT GIKDAGTIVA INKDGDAPIF EIADIGLVGD LFAILPELEK AL

NT Sequence

ATGAGCAAGA TTCTCGTCAT CGCCGAACAC CACGACGGCA AGCTCAATGC CGCCACCGCC AAGACTGTCA GCGCCGCCGC CGCCATCAGC GGTGCCAGCA TCGACGTGCT GGTGCTGGCC GCCGATCCGG CCGCTGTCGC CGCTGAAGCC GCGAAGATCG CTGGCGTCGC CAAGGTCCTG ACCGTGGCCA ATGCCGCCAA CGCGCAGGCC ATCGCCCAGG TGCTGGCGCC GCAGATCGCA CAGCTGGCCA AGGGCTACAC CCACGTGTTC GGCCCGTCGA CCACCTTCGG CAAGGACCTG ATGCCGTGCG TGGCCGCGCT GCTCGGCGTC AACCAGGTCT CCGACCTGAT GACCGTCGAA GGCAGCCACA CCTTCAAGCG CCCGATCTAC GCCGGCAACG CGATCATCAC CGTGGAAGCC CCAGCCGACC AGATCGTGGT TGCCACCGTG CGTGCCGCGT CGTGGCCGGA AGCCGCCCAG GGCGGCAGCG CCGCCATTGA AGCGGCGAGC GTCGACGCCG CACTGCCAAC CCACACCCGC TTCGTCGGCC TCGCCGCCGG TGCCAGCGAC CGCCCGGACC TGCAGAGCGC CAAGCGCGTG GTCTCCGGCG GCCGTGGCGT CGGCTCGGAA GAGAACTTCA AGGTCATCTT CCAGCTGGCC GACAAGCTGG GTGCCGCCGT CGGTGCCTCG CGCGCCGCGG TCGATGCCGG TTACGTGCCC AGCGACCTGC AGGTCGGCCA GACCGGCAAG ATCATCGCGC CGGAACTGTA CGTGGCCGTG GGTATCAGCG GCGCGATCCA GCACCTGACC GGAATCAAGG ATGCCGGCAC CATCGTGGCC ATCAACAAGG ACGGCGATGC GCCGATCTTT GAGATTGCCG ATATCGGCCT GGTGGGGGAT CTGTTTGCGA TCCTGCCGGA GCTGGAAAAG GCGCTC

Details for StmaA.17867.a.B1.GE43419

HARVESTED ON:	9/18/2019
SEQUENCED ON:	10/1/2019
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS KILVIAEHHD GKLNAATAKT VSAAAAISGA SIDVLVLAAD PAAVAAEAAK IAGVAKVLTV ANAANAQAIA QVLAPQIAQL AKGYTHVFGP STTFGKDLMP CVAALLGVNQ VSDLMTVEGS HTFKRPIYAG NAIITVEAPA DQIVVATVRA ASWPEAAQGG SAAIEAASVD AALPTHTRFV GLAAGASDRP DLQSAKRVVS GGRGVGSEEN FKVIFQLADK LGAAVGASRA AVDAGYVPSD LQVGQTGKII APELYVAVGI SGAIQHLTGI KDAGTIVAIN KDGDAPIFEI ADIGLVGDLF XXL

Validated NT Sequence

gcaggntcnn aaacagatcc cccaccaggc cgatatcggc aatctcaaag atcggcgcat cgccgtcctt gttgatggcc acgatggtgc cggcatcctt gattccggtc aggtgctgga tcgcgccgct gatacccacg gccacgtaca gttccggcgc gatgatcttg ccggtctggc cgacctgcag gtcgctgggc acgtaaccgg catcgaccgc ggcgcgcgag gcaccgacgg cggcacccag cttgtcggcc agctggaaga tgaccttgaa gttctcttcc gagccgacgc cacggccgcc ggagaccacg cgcttggcgc tctgcaggtc cgggcggtcg ctggcaccgg cggcgaggcc gacgaagcgg gtgtgggttg gcagtgcggc gtcgacgctc gccgcttcaa tggcggcgct gccgccctgg gcggcttccg gccacgacgc ggcacgcacg gtggcaacca cgatctggtc ggctggggct tccacggtga tgatcgcgtt gccggcgtag atcgggcgct tgaaggtgtg gctgccttcg acggtcatca ggtcggagac ctggttgacg ccgagcagcg cggccacgca cggcatcagg tccttgccga aggtggtcga cgggccgaac acgtgggtgt agcccttggc cagctgtgcg atctgcggcg ccagcacctg ggcgatggcc tgcgcgttgg cggcattggc cacggtcagg accttggcga cgccagcgat cttcgcggct tcagcggcga cagcggccgg atcggcggcc agcaccagca cgtcgatgct ggcaccgctg atggcggcgg cggcgctgac agtcttggcg gtggcggcat tgagcttgcc gtcgtggtgt tcggcgatga cgagaatctt gctcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agattctcgt catcgccgaa caccacgacg gcaagctcaa tgccgccacc gccaagactg tcagcgccgc cgccgccatc agcggtgcca gcatcgacgt gctggtgctg gccgccgatc cggccgctgt cgccgctgaa gccgcgaaga tcgctggcgt cgccaaggtc ctgaccgtgg ccaatgccgc caacgcgcag gccatcgccc aggtgctggc gccgcagatc gcacagctgg ccaagggcta cacccacgtg ttcggcccgt cgaccacctt cggcaaggac ctgatgccgt gcgtggccgc gctgctcggc gtcaaccagg tctccgacct gatgaccgtc gaaggcagcc acaccttcaa gcgcccgatc tacgccggca acgcgatcat caccgtggaa gccccagccg accagatcgt ggttgccacc gtgcgtgccg cgtcgtggcc ggaagccgcc cagggcggca gcgccgccat tgaagcggcg agcgtcgacg ccgcactgcc aacccacacc cgcttcgtcg gcctcgccgc cggtgccagc gaccgcccgg acctgcagag cgccaagcgc gtggtctccg gcggccgtgg cgtcggctcg gaagagaact tcaaggtcat cttccagctg gccgacaagc tgggtgccgc cgtcggtgcc tcgcgcgccg cggtcgatgc cggttacgtg cccagcgacc tgcaggtcgg ccagaccggc aagatcatcg cgccggaact gtacgtggcc gtgggtatca gcggcgcgat ccagcacctg accggaatca aggatgccgg caccatcgtg gccatcaaca aggacggcga tgcgccgatc tttgagattg ccgatatcgg cctggtgggg gatctgtttg cgatcctgcc ggagctggaa aaggcgctct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat