StmaA.17369.c

ATP synthase alpha chain (EC 3.6.3.14) (atpA)

CENTER ID: StmaA.17369.c
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17369.c.B11.GE43416 Full length(StmaA.17369.c) 1 515
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.17369.c.B11.PW38758 Full length(StmaA.17369.c) 1 515
StmaA.17369.c.B11.PW38759 Full length(StmaA.17369.c) 1 515

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3884

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATTLNPSEI SELIKNRIEK VKLAAESRNE GTVTSVSDGI VRIFGLADVM QGEMIELPNN TFALALNLER DSVGAVVLGD YEHLREGDVA KTTGRILEVP VGPELLGRVV NALGEPIDGK GPLGTDLTAP VERVAPGVIW RKSVDQPVQT GYKSVDSMIP IGRGQRELII GDRQTGKTAM AIDAVINQKG TGIKCVYVAI GQKASTIANI VRKLEENGAL AHTIVVAATA SESAAMQYIS AYSGCTMGEY FMDRGEDALI VYDDLSKQAV AYRQISLLLK RPPGREAYPG DVFYLHSRLL ERAARVSEEY VEKFTEGKVT GKTGSLTALP IIETQAGDVS AFVPTNVISI TDGQIFLETD LFNAGIRPAV NAGISVSRVG GSAQTKIIKK LSGGIRISLA QYRELAAFAQ FASDLDEATR KQLERGQRVT ELMKQKQYAP MSIANQALSI YAVNEGYLDD VPVNKLLAFE EGLHAHFANT QGELVSKVNA TGGWDNDIEG AFKKGIAEFK TTGSW
NT Sequence
ATGGCAACCA CGCTCAACCC CTCCGAAATC AGCGAACTGA TCAAGAACCG CATCGAGAAG GTCAAGCTGG CCGCGGAATC GCGCAACGAA GGCACCGTGA CCAGCGTGTC CGACGGCATC GTGCGCATCT TCGGTCTGGC CGACGTGATG CAGGGCGAAA TGATCGAACT GCCGAACAAC ACCTTCGCCC TCGCCCTGAA CCTGGAGCGC GACTCGGTCG GCGCCGTGGT GCTGGGTGAC TACGAGCACC TGCGCGAAGG CGACGTCGCC AAGACCACCG GCCGCATCCT GGAAGTGCCG GTCGGTCCGG AACTGCTGGG CCGCGTCGTG AACGCGCTGG GCGAGCCGAT CGACGGCAAG GGCCCGCTGG GCACCGACCT GACCGCTCCG GTGGAGCGTG TTGCGCCGGG CGTGATCTGG CGCAAGTCGG TCGACCAGCC GGTGCAGACC GGCTACAAGT CGGTCGACTC GATGATCCCG ATCGGCCGTG GCCAGCGCGA GCTGATCATC GGTGACCGCC AGACCGGCAA GACCGCGATG GCGATCGATG CCGTGATCAA CCAGAAGGGC ACCGGCATCA AGTGCGTATA CGTTGCGATC GGCCAGAAGG CTTCGACCAT CGCCAACATC GTGCGCAAGC TGGAAGAGAA CGGCGCGCTG GCCCACACCA TCGTGGTGGC CGCGACCGCT TCCGAATCGG CTGCCATGCA GTACATCAGC GCCTACTCGG GCTGCACCAT GGGTGAGTAC TTCATGGACC GTGGCGAAGA CGCGCTGATC GTGTACGACG ATCTGTCCAA GCAGGCTGTC GCCTACCGCC AGATCTCGCT GCTGCTGAAG CGCCCGCCGG GCCGTGAAGC CTACCCGGGT GACGTGTTCT ACCTGCACTC CCGTCTGCTC GAGCGCGCTG CCCGCGTGTC CGAGGAATAC GTCGAGAAGT TCACCGAAGG CAAGGTCACC GGCAAGACCG GTTCGCTGAC CGCGCTGCCG ATCATCGAAA CCCAGGCCGG CGACGTGTCC GCCTTCGTTC CGACCAACGT GATCTCGATC ACCGACGGCC AGATCTTCCT GGAAACCGAT CTGTTCAACG CCGGCATCCG CCCGGCGGTG AACGCCGGTA TCTCGGTGTC GCGCGTCGGT GGCTCGGCCC AGACCAAGAT CATCAAGAAG CTGTCCGGCG GCATCCGTAT CTCGCTGGCC CAGTACCGCG AGCTGGCTGC GTTCGCGCAG TTCGCCTCGG ACCTGGACGA AGCGACCCGC AAGCAGCTGG AGCGTGGCCA GCGCGTCACC GAGCTGATGA AGCAGAAGCA GTACGCGCCG ATGTCGATCG CCAACCAGGC ACTGTCGATC TACGCCGTCA ACGAGGGTTA CCTCGACGAC GTGCCGGTCA ACAAGCTGCT GGCGTTCGAA GAAGGCCTGC ACGCCCACTT CGCCAACACC CAGGGCGAGC TGGTCAGCAA GGTCAACGCC ACCGGCGGTT GGGACAACGA CATCGAAGGT GCCTTCAAGA AGGGCATCGC CGAGTTCAAG ACCACCGGCA GCTGG
Details for StmaA.17369.c.B11.GE43416
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA TTLNPSEISE LIKNRIEKVK LAAESRNEGT VTSVSDGIVR IFGLADVMQG EMIELPNNTF ALALNLERDS VGAVVLGDYE HLREGDVAKT TGRILEVPVG PELLGRVVNA LGEPIDGKGP LGTDLTAPVE RVAPGVIWRK SVDQPVQTGY KSVDSMIPIG RGQRELIIGD RQTGKTAMAI DAVINQKGTG IKCVYVAIGQ KASTIANIVR KLEENGALAH TIVVAATASE SAAMQYISAY SGCTMGEYFM DRGEDALIVY DDLSKQAVAY RQISLLLKRP PGREAYPGDV FYLHSRLLER AARVSEEYVE KFTEGKVTGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETDLF NAGIRPAVNA GISVSRVGGS AQTKIIKKLS GGIRISLAQY RELAAFAQFA SDLDEATRKQ LERGQRVTEL MKQKQYAPMS IANQALSIYA VNEGYLDDVP VNKLLAFEEG LHAHFANTQG ELVSKVNATG GWDNDIEGAF KK
Validated NT Sequence
gcccttcttg aaggcacctt cgatgtcgtt gtcccaaccg ccggtggcgt tgaccttgct gaccagctcg ccctgggtgt tggcgaagtg ggcgtgcagg ccttcttcga acgccagcag cttgttgacc ggcacgtcgt cgaggtaacc ctcgttgacg gcgtagatcg acagtgcctg gttggcgatc gacatcggcg cgtactgctt ctgcttcatc agctcggtga cgcgctggcc acgctccagc tgcttgcggg tcgcttcgtc caggtccgag gcgaactgcg cgaacgcagc cagctcgcgg tactgggcca gcgagatacg gatgccgccg gacagcttct tgatgatctt ggtctgggcc gagccaccga cgcgcgacac cgagataccg gcgttcaccg ccgggcggat gccggcgttg aacagatcgg tttccaggaa gatctggccg tcggtgatcg agatcacgtt ggtcggaacg aaggcggaca cgtcgccggc ctgggtttcg atgatcggca gcgcggtcag cgaaccggtc ttgccggtga ccttgccttc ggtgaacttc tcgacgtatt cctcggacac gcgggcagcg cgctcgagca gacgggagtg caggtagaac acgtcacccg ggtaggcttc acggcccggc gggcgcttca gcagcagcga gatctggcgg taggcgacag cctgcttgga cagatcgtcg tacacgatca gcgcgtcttc gccacggtcc atgaagtact cacccatggt gcagcccgag taggcgctga tgtactgcat ggcagccgat tcggaagcgg tcgcggccac cacgatggtg tgggccagcg cgccgttctc ttccagcttg cgcacgatgt tggcgatggt cgaagccttc tggccgatcg caacgtatac gcacttgatg ccggtgccct tctggttgat cacggcatcg atcgccatcg cggtcttgcc ggtctggcgg tcaccgatga tcagctcgcg ctggccacgg ccgatcggga tcatcgagtc gaccgacttg tagccggtct gcaccggctg gtcgaccgac ttgcgccaga tcacgcccgg cgcaacacgc tccaccggag cggtcaggtc ggtgcccagc gggcccttgc cgtcgatcgg ctcgcccagc gcgttcacga cgcggcccag cagttccgga ccgaccggca cttccaggat gcggccggtg gtcttggcga cgtcgccttc gcgcaggtgc tcgtagtcac ccagcaccac ggcgccgacc gagtcgcgct ccaggttcag ggcgagggcg aaggtgttgt tcggcagttc gatcatttcg ccctgcatca cgtcggccag accgaagatg cgcacgatgc cgtcggacac gctggtcacg gtgccttcgt tgcgcgattc cgcggccagc ttgaccttct cgatgcggtt cttgatcagt tcgctgattt cggaggggtt gagcgtggtt gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMA TTLNPSEISE LIKNRIEKVK LAAESRNEGT VTSVSDGIVR IFGLADVMQG EMIELPNNTF ALALNLERDS VGAVVLGDYE HLREGDVAKT TGRILEVPVG PELLGRVVNA LGEPIDGKGP LGTDLTAPVE RVAPGVIWRK SVDQPVQTGY KSVDSMIPIG RGQRELIIGD RQTGKTAMAI DAVINQKGTG IKCVYVAIGQ KASTIANIVR KLEENGALAH TIVVAATASE SAAMQYISAY SGCTMGEYFM DRGEDALIVY DDLSKQAVAY RQISLLLKRP PGREAYPGDV FYLHSRLLER AARVSEEYVE KFTEGKVTGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETDLF NAGIRPAVNA GISVSRVGGS AQTKIIKKLS GGIRISLAQY RELAAFAQFA SDLDEATRKQ LERGQRVTEL MKQKQYAPMS IANQALSIYA VNEGYLDDVP VNKLLAFEEG LHAHFANTQG ELVSKVNATG GWDNDIEGAF KKGIAEFKTT GSW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa ccacgctcaa cccctccgaa atcagcgaac tgatcaagaa ccgcatcgag aaggtcaagc tggccgcgga atcgcgcaac gaaggcaccg tgaccagcgt gtccgacggc atcgtgcgca tcttcggtct ggccgacgtg atgcagggcg aaatgatcga actgccgaac aacaccttcg ccctcgccct gaacctggag cgcgactcgg tcggcgccgt ggtgctgggt gactacgagc acctgcgcga aggcgacgtc gccaagacca ccggccgcat cctggaagtg ccggtcggtc cggaactgct gggccgcgtc gtgaacgcgc tgggcgagcc gatcgacggc aagggcccgc tgggcaccga cctgaccgct ccggtggagc gtgttgcgcc gggcgtgatc tggcgcaagt cggtcgacca gccggtgcag accggctaca agtcggtcga ctcgatgatc ccgatcggcc gtggccagcg cgagctgatc atcggtgacc gccagaccgg caagaccgcg atggcgatcg atgccgtgat caaccagaag ggcaccggca tcaagtgcgt atacgttgcg atcggccaga aggcttcgac catcgccaac atcgtgcgca agctggaaga gaacggcgcg ctggcccaca ccatcgtggt ggccgcgacc gcttccgaat cggctgccat gcagtacatc agcgcctact cgggctgcac catgggtgag tacttcatgg accgtggcga agacgcgctg atcgtgtacg acgatctgtc caagcaggct gtcgcctacc gccagatctc gctgctgctg aagcgcccgc cgggccgtga agcctacccg ggtgacgtgt tctacctgca ctcccgtctg ctcgagcgcg ctgcccgcgt gtccgaggaa tacgtcgaga agttcaccga aggcaaggtc accggcaaga ccggttcgct gaccgcgctg ccgatcatcg aaacccaggc cggcgacgtg tccgccttcg ttccgaccaa cgtgatctcg atcaccgacg gccagatctt cctggaaacc gatctgttca acgccggcat ccgcccggcg gtgaacgccg gtatctcggt gtcgcgcgtc ggtggctcgg cccagaccaa gatcatcaag aagctgtccg gcggcatccg tatctcgctg gcccagtacc gcgagctggc tgcgttcgcg cagttcgcct cggacctgga cgaagcgacc cgcaagcagc tggagcgtgg ccagcgcgtc accgagctga tgaagcagaa gcagtacgcg ccgatgtcga tcgccaacca ggcactgtcg atctacgccg tcaacgaggg ttacctcgac gacgtgccgg tcaacaagct gctggcgttc gaagaaggcc tgcacgccca cttcgccaac acccagggcg agctggtcag caaggtcaac gccaccggcg gttgggacaa cgacatcgaa ggtgccttca agaagggcat cgccgagttc aagaccaccg gcagctggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for StmaA.17369.c.B11.PW38758
PURIFICATION DATe: 11/21/2019
CONCENTRATION: 18mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA TTLNPSEISE LIKNRIEKVK LAAESRNEGT VTSVSDGIVR IFGLADVMQG EMIELPNNTF ALALNLERDS VGAVVLGDYE HLREGDVAKT TGRILEVPVG PELLGRVVNA LGEPIDGKGP LGTDLTAPVE RVAPGVIWRK SVDQPVQTGY KSVDSMIPIG RGQRELIIGD RQTGKTAMAI DAVINQKGTG IKCVYVAIGQ KASTIANIVR KLEENGALAH TIVVAATASE SAAMQYISAY SGCTMGEYFM DRGEDALIVY DDLSKQAVAY RQISLLLKRP PGREAYPGDV FYLHSRLLER AARVSEEYVE KFTEGKVTGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETDLF NAGIRPAVNA GISVSRVGGS AQTKIIKKLS GGIRISLAQY RELAAFAQFA SDLDEATRKQ LERGQRVTEL MKQKQYAPMS IANQALSIYA VNEGYLDDVP VNKLLAFEEG LHAHFANTQG ELVSKVNATG GWDNDIEGAF KK
Validated NT Sequence
gcccttcttg aaggcacctt cgatgtcgtt gtcccaaccg ccggtggcgt tgaccttgct gaccagctcg ccctgggtgt tggcgaagtg ggcgtgcagg ccttcttcga acgccagcag cttgttgacc ggcacgtcgt cgaggtaacc ctcgttgacg gcgtagatcg acagtgcctg gttggcgatc gacatcggcg cgtactgctt ctgcttcatc agctcggtga cgcgctggcc acgctccagc tgcttgcggg tcgcttcgtc caggtccgag gcgaactgcg cgaacgcagc cagctcgcgg tactgggcca gcgagatacg gatgccgccg gacagcttct tgatgatctt ggtctgggcc gagccaccga cgcgcgacac cgagataccg gcgttcaccg ccgggcggat gccggcgttg aacagatcgg tttccaggaa gatctggccg tcggtgatcg agatcacgtt ggtcggaacg aaggcggaca cgtcgccggc ctgggtttcg atgatcggca gcgcggtcag cgaaccggtc ttgccggtga ccttgccttc ggtgaacttc tcgacgtatt cctcggacac gcgggcagcg cgctcgagca gacgggagtg caggtagaac acgtcacccg ggtaggcttc acggcccggc gggcgcttca gcagcagcga gatctggcgg taggcgacag cctgcttgga cagatcgtcg tacacgatca gcgcgtcttc gccacggtcc atgaagtact cacccatggt gcagcccgag taggcgctga tgtactgcat ggcagccgat tcggaagcgg tcgcggccac cacgatggtg tgggccagcg cgccgttctc ttccagcttg cgcacgatgt tggcgatggt cgaagccttc tggccgatcg caacgtatac gcacttgatg ccggtgccct tctggttgat cacggcatcg atcgccatcg cggtcttgcc ggtctggcgg tcaccgatga tcagctcgcg ctggccacgg ccgatcggga tcatcgagtc gaccgacttg tagccggtct gcaccggctg gtcgaccgac ttgcgccaga tcacgcccgg cgcaacacgc tccaccggag cggtcaggtc ggtgcccagc gggcccttgc cgtcgatcgg ctcgcccagc gcgttcacga cgcggcccag cagttccgga ccgaccggca cttccaggat gcggccggtg gtcttggcga cgtcgccttc gcgcaggtgc tcgtagtcac ccagcaccac ggcgccgacc gagtcgcgct ccaggttcag ggcgagggcg aaggtgttgt tcggcagttc gatcatttcg ccctgcatca cgtcggccag accgaagatg cgcacgatgc cgtcggacac gctggtcacg gtgccttcgt tgcgcgattc cgcggccagc ttgaccttct cgatgcggtt cttgatcagt tcgctgattt cggaggggtt gagcgtggtt gccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMA TTLNPSEISE LIKNRIEKVK LAAESRNEGT VTSVSDGIVR IFGLADVMQG EMIELPNNTF ALALNLERDS VGAVVLGDYE HLREGDVAKT TGRILEVPVG PELLGRVVNA LGEPIDGKGP LGTDLTAPVE RVAPGVIWRK SVDQPVQTGY KSVDSMIPIG RGQRELIIGD RQTGKTAMAI DAVINQKGTG IKCVYVAIGQ KASTIANIVR KLEENGALAH TIVVAATASE SAAMQYISAY SGCTMGEYFM DRGEDALIVY DDLSKQAVAY RQISLLLKRP PGREAYPGDV FYLHSRLLER AARVSEEYVE KFTEGKVTGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETDLF NAGIRPAVNA GISVSRVGGS AQTKIIKKLS GGIRISLAQY RELAAFAQFA SDLDEATRKQ LERGQRVTEL MKQKQYAPMS IANQALSIYA VNEGYLDDVP VNKLLAFEEG LHAHFANTQG ELVSKVNATG GWDNDIEGAF KKGIAEFKTT GSW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA CCACGCTCAA CCCCTCCGAA ATCAGCGAAC TGATCAAGAA CCGCATCGAG AAGGTCAAGC TGGCCGCGGA ATCGCGCAAC GAAGGCACCG TGACCAGCGT GTCCGACGGC ATCGTGCGCA TCTTCGGTCT GGCCGACGTG ATGCAGGGCG AAATGATCGA ACTGCCGAAC AACACCTTCG CCCTCGCCCT GAACCTGGAG CGCGACTCGG TCGGCGCCGT GGTGCTGGGT GACTACGAGC ACCTGCGCGA AGGCGACGTC GCCAAGACCA CCGGCCGCAT CCTGGAAGTG CCGGTCGGTC CGGAACTGCT GGGCCGCGTC GTGAACGCGC TGGGCGAGCC GATCGACGGC AAGGGCCCGC TGGGCACCGA CCTGACCGCT CCGGTGGAGC GTGTTGCGCC GGGCGTGATC TGGCGCAAGT CGGTCGACCA GCCGGTGCAG ACCGGCTACA AGTCGGTCGA CTCGATGATC CCGATCGGCC GTGGCCAGCG CGAGCTGATC ATCGGTGACC GCCAGACCGG CAAGACCGCG ATGGCGATCG ATGCCGTGAT CAACCAGAAG GGCACCGGCA TCAAGTGCGT ATACGTTGCG ATCGGCCAGA AGGCTTCGAC CATCGCCAAC ATCGTGCGCA AGCTGGAAGA GAACGGCGCG CTGGCCCACA CCATCGTGGT GGCCGCGACC GCTTCCGAAT CGGCTGCCAT GCAGTACATC AGCGCCTACT CGGGCTGCAC CATGGGTGAG TACTTCATGG ACCGTGGCGA AGACGCGCTG ATCGTGTACG ACGATCTGTC CAAGCAGGCT GTCGCCTACC GCCAGATCTC GCTGCTGCTG AAGCGCCCGC CGGGCCGTGA AGCCTACCCG GGTGACGTGT TCTACCTGCA CTCCCGTCTG CTCGAGCGCG CTGCCCGCGT GTCCGAGGAA TACGTCGAGA AGTTCACCGA AGGCAAGGTC ACCGGCAAGA CCGGTTCGCT GACCGCGCTG CCGATCATCG AAACCCAGGC CGGCGACGTG TCCGCCTTCG TTCCGACCAA CGTGATCTCG ATCACCGACG GCCAGATCTT CCTGGAAACC GATCTGTTCA ACGCCGGCAT CCGCCCGGCG GTGAACGCCG GTATCTCGGT GTCGCGCGTC GGTGGCTCGG CCCAGACCAA GATCATCAAG AAGCTGTCCG GCGGCATCCG TATCTCGCTG GCCCAGTACC GCGAGCTGGC TGCGTTCGCG CAGTTCGCCT CGGACCTGGA CGAAGCGACC CGCAAGCAGC TGGAGCGTGG CCAGCGCGTC ACCGAGCTGA TGAAGCAGAA GCAGTACGCG CCGATGTCGA TCGCCAACCA GGCACTGTCG ATCTACGCCG TCAACGAGGG TTACCTCGAC GACGTGCCGG TCAACAAGCT GCTGGCGTTC GAAGAAGGCC TGCACGCCCA CTTCGCCAAC ACCCAGGGCG AGCTGGTCAG CAAGGTCAAC GCCACCGGCG GTTGGGACAA CGACATCGAA GGTGCCTTCA AGAAGGGCAT CGCCGAGTTC AAGACCACCG GCAGCTGGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for StmaA.17369.c.B11.PW38759
PURIFICATION DATe: 11/21/2019
CONCENTRATION: 15.7mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA TTLNPSEISE LIKNRIEKVK LAAESRNEGT VTSVSDGIVR IFGLADVMQG EMIELPNNTF ALALNLERDS VGAVVLGDYE HLREGDVAKT TGRILEVPVG PELLGRVVNA LGEPIDGKGP LGTDLTAPVE RVAPGVIWRK SVDQPVQTGY KSVDSMIPIG RGQRELIIGD RQTGKTAMAI DAVINQKGTG IKCVYVAIGQ KASTIANIVR KLEENGALAH TIVVAATASE SAAMQYISAY SGCTMGEYFM DRGEDALIVY DDLSKQAVAY RQISLLLKRP PGREAYPGDV FYLHSRLLER AARVSEEYVE KFTEGKVTGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETDLF NAGIRPAVNA GISVSRVGGS AQTKIIKKLS GGIRISLAQY RELAAFAQFA SDLDEATRKQ LERGQRVTEL MKQKQYAPMS IANQALSIYA VNEGYLDDVP VNKLLAFEEG LHAHFANTQG ELVSKVNATG GWDNDIEGAF KK
Validated NT Sequence
gcccttcttg aaggcacctt cgatgtcgtt gtcccaaccg ccggtggcgt tgaccttgct gaccagctcg ccctgggtgt tggcgaagtg ggcgtgcagg ccttcttcga acgccagcag cttgttgacc ggcacgtcgt cgaggtaacc ctcgttgacg gcgtagatcg acagtgcctg gttggcgatc gacatcggcg cgtactgctt ctgcttcatc agctcggtga cgcgctggcc acgctccagc tgcttgcggg tcgcttcgtc caggtccgag gcgaactgcg cgaacgcagc cagctcgcgg tactgggcca gcgagatacg gatgccgccg gacagcttct tgatgatctt ggtctgggcc gagccaccga cgcgcgacac cgagataccg gcgttcaccg ccgggcggat gccggcgttg aacagatcgg tttccaggaa gatctggccg tcggtgatcg agatcacgtt ggtcggaacg aaggcggaca cgtcgccggc ctgggtttcg atgatcggca gcgcggtcag cgaaccggtc ttgccggtga ccttgccttc ggtgaacttc tcgacgtatt cctcggacac gcgggcagcg cgctcgagca gacgggagtg caggtagaac acgtcacccg ggtaggcttc acggcccggc gggcgcttca gcagcagcga gatctggcgg taggcgacag cctgcttgga cagatcgtcg tacacgatca gcgcgtcttc gccacggtcc atgaagtact cacccatggt gcagcccgag taggcgctga tgtactgcat ggcagccgat tcggaagcgg tcgcggccac cacgatggtg tgggccagcg cgccgttctc ttccagcttg cgcacgatgt tggcgatggt cgaagccttc tggccgatcg caacgtatac gcacttgatg ccggtgccct tctggttgat cacggcatcg atcgccatcg cggtcttgcc ggtctggcgg tcaccgatga tcagctcgcg ctggccacgg ccgatcggga tcatcgagtc gaccgacttg tagccggtct gcaccggctg gtcgaccgac ttgcgccaga tcacgcccgg cgcaacacgc tccaccggag cggtcaggtc ggtgcccagc gggcccttgc cgtcgatcgg ctcgcccagc gcgttcacga cgcggcccag cagttccgga ccgaccggca cttccaggat gcggccggtg gtcttggcga cgtcgccttc gcgcaggtgc tcgtagtcac ccagcaccac ggcgccgacc gagtcgcgct ccaggttcag ggcgagggcg aaggtgttgt tcggcagttc gatcatttcg ccctgcatca cgtcggccag accgaagatg cgcacgatgc cgtcggacac gctggtcacg gtgccttcgt tgcgcgattc cgcggccagc ttgaccttct cgatgcggtt cttgatcagt tcgctgattt cggaggggtt gagcgtggtt gccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMA TTLNPSEISE LIKNRIEKVK LAAESRNEGT VTSVSDGIVR IFGLADVMQG EMIELPNNTF ALALNLERDS VGAVVLGDYE HLREGDVAKT TGRILEVPVG PELLGRVVNA LGEPIDGKGP LGTDLTAPVE RVAPGVIWRK SVDQPVQTGY KSVDSMIPIG RGQRELIIGD RQTGKTAMAI DAVINQKGTG IKCVYVAIGQ KASTIANIVR KLEENGALAH TIVVAATASE SAAMQYISAY SGCTMGEYFM DRGEDALIVY DDLSKQAVAY RQISLLLKRP PGREAYPGDV FYLHSRLLER AARVSEEYVE KFTEGKVTGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETDLF NAGIRPAVNA GISVSRVGGS AQTKIIKKLS GGIRISLAQY RELAAFAQFA SDLDEATRKQ LERGQRVTEL MKQKQYAPMS IANQALSIYA VNEGYLDDVP VNKLLAFEEG LHAHFANTQG ELVSKVNATG GWDNDIEGAF KKGIAEFKTT GSW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA CCACGCTCAA CCCCTCCGAA ATCAGCGAAC TGATCAAGAA CCGCATCGAG AAGGTCAAGC TGGCCGCGGA ATCGCGCAAC GAAGGCACCG TGACCAGCGT GTCCGACGGC ATCGTGCGCA TCTTCGGTCT GGCCGACGTG ATGCAGGGCG AAATGATCGA ACTGCCGAAC AACACCTTCG CCCTCGCCCT GAACCTGGAG CGCGACTCGG TCGGCGCCGT GGTGCTGGGT GACTACGAGC ACCTGCGCGA AGGCGACGTC GCCAAGACCA CCGGCCGCAT CCTGGAAGTG CCGGTCGGTC CGGAACTGCT GGGCCGCGTC GTGAACGCGC TGGGCGAGCC GATCGACGGC AAGGGCCCGC TGGGCACCGA CCTGACCGCT CCGGTGGAGC GTGTTGCGCC GGGCGTGATC TGGCGCAAGT CGGTCGACCA GCCGGTGCAG ACCGGCTACA AGTCGGTCGA CTCGATGATC CCGATCGGCC GTGGCCAGCG CGAGCTGATC ATCGGTGACC GCCAGACCGG CAAGACCGCG ATGGCGATCG ATGCCGTGAT CAACCAGAAG GGCACCGGCA TCAAGTGCGT ATACGTTGCG ATCGGCCAGA AGGCTTCGAC CATCGCCAAC ATCGTGCGCA AGCTGGAAGA GAACGGCGCG CTGGCCCACA CCATCGTGGT GGCCGCGACC GCTTCCGAAT CGGCTGCCAT GCAGTACATC AGCGCCTACT CGGGCTGCAC CATGGGTGAG TACTTCATGG ACCGTGGCGA AGACGCGCTG ATCGTGTACG ACGATCTGTC CAAGCAGGCT GTCGCCTACC GCCAGATCTC GCTGCTGCTG AAGCGCCCGC CGGGCCGTGA AGCCTACCCG GGTGACGTGT TCTACCTGCA CTCCCGTCTG CTCGAGCGCG CTGCCCGCGT GTCCGAGGAA TACGTCGAGA AGTTCACCGA AGGCAAGGTC ACCGGCAAGA CCGGTTCGCT GACCGCGCTG CCGATCATCG AAACCCAGGC CGGCGACGTG TCCGCCTTCG TTCCGACCAA CGTGATCTCG ATCACCGACG GCCAGATCTT CCTGGAAACC GATCTGTTCA ACGCCGGCAT CCGCCCGGCG GTGAACGCCG GTATCTCGGT GTCGCGCGTC GGTGGCTCGG CCCAGACCAA GATCATCAAG AAGCTGTCCG GCGGCATCCG TATCTCGCTG GCCCAGTACC GCGAGCTGGC TGCGTTCGCG CAGTTCGCCT CGGACCTGGA CGAAGCGACC CGCAAGCAGC TGGAGCGTGG CCAGCGCGTC ACCGAGCTGA TGAAGCAGAA GCAGTACGCG CCGATGTCGA TCGCCAACCA GGCACTGTCG ATCTACGCCG TCAACGAGGG TTACCTCGAC GACGTGCCGG TCAACAAGCT GCTGGCGTTC GAAGAAGGCC TGCACGCCCA CTTCGCCAAC ACCCAGGGCG AGCTGGTCAG CAAGGTCAAC GCCACCGGCG GTTGGGACAA CGACATCGAA GGTGCCTTCA AGAAGGGCAT CGCCGAGTTC AAGACCACCG GCAGCTGGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT