SSGCID: Target Page

StmaA.17369.a

ATP synthase beta chain (EC 3.6.3.14) (atpD)

CENTER ID:	StmaA.17369.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.17369.a.B1.GE43384	Full length(StmaA.17369.a)		1	468

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3882

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQGKIVQII GAVVDVEFPR ESVPKVYDAL KVENTEITLE VQQQLGDGVV RTIALGSTDG LKRNLVAVNT GRGISVPVGA GTLGRIMDVL GRPIDEAGPV AASDSWEIHR AAPSYEDQSP ATELLETGIK VIDLMCPFAK GGKVGLFGGA GVGKTVNMME LINNIAKAHS GLSVFAGVGE RTREGNDFYH EMKDSNVLDK VAMVYGQMNE PPGNRLRVAL TGLTMAEYFR DEKDENGKGK DVLLFVDNIY RYTLAGTEVS ALLGRMPSAV GYQPTLAEEM GVLQERITST KNGSITSIQA VYVPADDLTD PSPATTFAHL DSTVTLSRSI ASLGIYPAVD PLDSTSRQMD PLVIGHEHYD TAQRVQQTLQ KYKELKDIIA ILGMDELSEE DKQAVSRARK IERFFSQPFH VAEVFTGSPG KYVPLKDTIR GFKAIVDGEY DHLPEQAFYM VGGIEEAVEK AKKMAEKA

NT Sequence

ATGAGTCAGG GCAAGATCGT TCAGATCATC GGCGCGGTCG TCGACGTCGA ATTCCCGCGT GAATCGGTGC CGAAGGTGTA CGACGCACTG AAGGTGGAAA ACACCGAGAT CACCCTCGAA GTCCAGCAGC AGCTGGGCGA CGGCGTGGTG CGTACCATCG CCCTCGGTTC CACCGACGGC CTGAAGCGCA ACCTGGTTGC CGTCAACACC GGCCGTGGCA TCTCGGTGCC GGTCGGCGCC GGCACCCTGG GCCGCATCAT GGACGTGCTC GGCCGTCCGA TCGACGAAGC CGGCCCGGTG GCCGCCAGCG ACAGCTGGGA AATCCACCGT GCGGCCCCGT CGTACGAAGA CCAGTCCCCG GCCACCGAAC TGCTGGAAAC CGGCATCAAG GTCATCGACC TGATGTGCCC GTTCGCCAAG GGCGGCAAGG TCGGCCTGTT CGGCGGCGCC GGCGTCGGCA AGACCGTCAA CATGATGGAG CTGATCAACA ACATCGCCAA GGCGCACAGC GGTCTGTCCG TGTTCGCCGG CGTGGGTGAG CGTACCCGTG AGGGCAACGA CTTCTACCAC GAAATGAAGG ACTCCAACGT CCTCGACAAG GTGGCGATGG TGTACGGCCA GATGAACGAG CCGCCGGGCA ACCGTCTGCG CGTCGCCCTG ACCGGCCTGA CCATGGCCGA GTACTTCCGC GACGAGAAGG ACGAAAACGG CAAGGGCAAG GACGTCCTGC TGTTCGTCGA CAACATCTAC CGCTACACCC TGGCCGGTAC CGAAGTGTCG GCACTGCTGG GCCGCATGCC GTCCGCCGTC GGTTACCAGC CGACCCTGGC CGAGGAAATG GGCGTCCTGC AGGAGCGCAT CACTTCGACC AAGAACGGTT CGATCACCTC GATCCAGGCC GTCTACGTTC CCGCGGACGA CCTGACCGAC CCGTCGCCGG CAACCACCTT CGCCCACCTG GACTCGACCG TTACCCTGTC GCGTTCGATC GCCTCGCTGG GTATCTACCC GGCCGTCGAT CCGCTGGACT CCACCAGCCG CCAGATGGAC CCGCTGGTCA TCGGCCACGA GCACTACGAC ACCGCCCAGC GCGTTCAGCA GACCCTGCAG AAGTACAAGG AACTGAAGGA CATCATCGCG ATCCTGGGCA TGGACGAACT GTCCGAAGAG GACAAGCAGG CCGTGTCGCG CGCCCGTAAG ATCGAGCGCT TCTTCAGCCA GCCGTTCCAC GTGGCCGAAG TGTTCACCGG TTCGCCGGGC AAGTACGTTC CGCTGAAGGA CACCATCCGT GGCTTCAAGG CCATCGTTGA TGGCGAGTAC GACCACCTGC CGGAGCAGGC GTTCTACATG GTTGGCGGCA TCGAAGAAGC GGTCGAGAAG GCCAAGAAGA TGGCCGAGAA GGCC

Details for StmaA.17369.a.B1.GE43384

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS QGKIVQIIGA VVDVEFPRES VPKVYDALKV ENTEITLEVQ QQLGDGVVRT IALGSTDGLK RNLVAVNTGR GISVPVGAGT LGRIMDVLGR PIDEAGPVAA SDSWEIHRAA PSYEDQSPAT ELLETGIKVI DLMCPFAKGG KVGLFGGAGV GKTVNMMELI NNIAKAHSGL SVFAGVGERT REGNDFYHEM KDSNVLDKVA MVYGQMNEPP GNRLRVALTG LTMAEYFRDE KDENGKGKDV LLFVDNIYRY TLAGTEVSAL LGRMPSAVGY QPTLAEEMGV LQERITSTKN GSITSIQAVY VPADDLTDPS PATTFAHLDS TVTLSRSIAS LGIYPAVDPL DSTSRQMDPL VIGHEHYDTA QRVQQTLQKY KELKDIIAIL GMDELSEEDK QAVSRARKIE RFFSQPFHVA EVFTGSPGKY VPLKDTIRGF KAIVDGEYDH LPEQAFYMVG GIEEAVE

Validated NT Sequence

tctcgaccgc ttcttcgatg ccgccaacca tgtagaacgc ctgctccggc aggtggtcgt actcgccatc aacgatggcc ttgaagccac ggatggtgtc cttcagcgga acgtacttgc ccggcgaacc ggtgaacact tcggccacgt ggaacggctg gctgaagaag cgctcgatct tacgggcgcg cgacacggcc tgcttgtcct cttcggacag ttcgtccatg cccaggatcg cgatgatgtc cttcagttcc ttgtacttct gcagggtctg ctgaacgcgc tgggcggtgt cgtagtgctc gtggccgatg accagcgggt ccatctggcg gctggtggag tccagcggat cgacggccgg gtagataccc agcgaggcga tcgaacgcga cagggtaacg gtcgagtcca ggtgggcgaa ggtggttgcc ggcgacgggt cggtcaggtc gtccgcggga acgtagacgg cctggatcga ggtgatcgaa ccgttcttgg tcgaagtgat gcgctcctgc aggacgccca tttcctcggc cagggtcggc tggtaaccga cggcggacgg catgcggccc agcagtgccg acacttcggt accggccagg gtgtagcggt agatgttgtc gacgaacagc aggacgtcct tgcccttgcc gttttcgtcc ttctcgtcgc ggaagtactc ggccatggtc aggccggtca gggcgacgcg cagacggttg cccggcggct cgttcatctg gccgtacacc atcgccacct tgtcgaggac gttggagtcc ttcatttcgt ggtagaagtc gttgccctca cgggtacgct cacccacgcc ggcgaacacg gacagaccgc tgtgcgcctt ggcgatgttg ttgatcagct ccatcatgtt gacggtcttg ccgacgccgg cgccgccgaa caggccgacc ttgccgccct tggcgaacgg gcacatcagg tcgatgacct tgatgccggt ttccagcagt tcggtggccg gggactggtc ttcgtacgac ggggccgcac ggtggatttc ccagctgtcg ctggcggcca ccgggccggc ttcgtcgatc ggacggccga gcacgtccat gatgcggccc agggtgccgg cgccgaccgg caccgagatg ccacggccgg tgttgacggc aaccaggttg cgcttcaggc cgtcggtgga accgagggcg atggtacgca ccacgccgtc gcccagctgc tgctggactt cgagggtgat ctcggtgttt tccaccttca gtgcgtcgta caccttcggc accgattcac gcgggaattc gacgtcgacg accgcgccga tgatctgaac gatcttgccc tgactcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc agggcaagat cgttcagatc atcggcgcgg tcgtcgacgt cgaattcccg cgtgaatcgg tgccgaaggt gtacgacgca ctgaaggtgg aaaacaccga gatcaccctc gaagtccagc agcagctggg cgacggcgtg gtgcgtacca tcgccctcgg ttccaccgac ggcctgaagc gcaacctggt tgccgtcaac accggccgtg gcatctcggt gccggtcggc gccggcaccc tgggccgcat catggacgtg ctcggccgtc cgatcgacga agccggcccg gtggccgcca gcgacagctg ggaaatccac cgtgcggccc cgtcgtacga agaccagtcc ccggccaccg aactgctgga aaccggcatc aaggtcatcg acctgatgtg cccgttcgcc aagggcggca aggtcggcct gttcggcggc gccggcgtcg gcaagaccgt caacatgatg gagctgatca acaacatcgc caaggcgcac agcggtctgt ccgtgttcgc cggcgtgggt gagcgtaccc gtgagggcaa cgacttctac cacgaaatga aggactccaa cgtcctcgac aaggtggcga tggtgtacgg ccagatgaac gagccgccgg gcaaccgtct gcgcgtcgcc ctgaccggcc tgaccatggc cgagtacttc cgcgacgaga aggacgaaaa cggcaagggc aaggacgtcc tgctgttcgt cgacaacatc taccgctaca ccctggccgg taccgaagtg tcggcactgc tgggccgcat gccgtccgcc gtcggttacc agccgaccct ggccgaggaa atgggcgtcc tgcaggagcg catcacttcg accaagaacg gttcgatcac ctcgatccag gccgtctacg ttcccgcgga cgacctgacc gacccgtcgc cggcaaccac cttcgcccac ctggactcga ccgttaccct gtcgcgttcg atcgcctcgc tgggtatcta cccggccgtc gatccgctgg actccaccag ccgccagatg gacccgctgg tcatcggcca cgagcactac gacaccgccc agcgcgttca gcagaccctg cagaagtaca aggaactgaa ggacatcatc gcgatcctgg gcatggacga actgtccgaa gaggacaagc aggccgtgtc gcgcgcccgt aagatcgagc gcttcttcag ccagccgttc cacgtggccg aagtgttcac cggttcgccg ggcaagtacg ttccgctgaa ggacaccatc cgtggcttca aggccatcgt tgatggcgag tacgaccacc tgccggagca ggcgttctac atggttggcg gcatcgaaga agcggtcgag aaggccaaga agatggccga gaaggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at