SSGCID: Target Page

StmaA.17266.a

Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8)

CENTER ID:	StmaA.17266.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.17266.a.B1.GE43383	Full length(StmaA.17266.a)		1	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.2606

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSLPKFLPI TVIDSAAPVP AEAHSHHHDA AAQGPRSLGQ PAPCRLFVDE TAISEADIAR EMQHHRAMRP EQSRAEAARA LVVRELLRLE AQRLGLQAPE GNRVSDEEVL IQQLLEDAIK DRVPSDEDCR RYFEQNPERF RSPDRVRLRH ILLAAPADDV AGRFAARTEG ERLVGLLKES PHLFADFALR HSRCPSSSEG GDLGWLQRGQ TTPEFDRQVF RLREGLAGFP VESRWGYHVV CVDAREEGQP QPFEAVLPQL RDYLELQVRQ REVQAYLLQL QERYPVRGLD EIEAEADIG

NT Sequence

ATGGGCAGCC TGCCGAAATT CCTGCCGATC ACCGTGATCG ACTCCGCTGC GCCGGTTCCC GCCGAGGCGC ATTCGCACCA CCATGACGCG GCAGCGCAGG GGCCGCGCTC GCTCGGGCAG CCGGCACCGT GCCGGCTGTT CGTGGACGAG ACCGCGATCA GCGAGGCCGA CATCGCCCGC GAGATGCAGC ACCACCGGGC GATGCGCCCG GAGCAGTCTC GTGCCGAAGC GGCACGTGCA CTGGTGGTGC GCGAGCTGCT GCGCCTGGAA GCGCAGCGGC TTGGGCTGCA GGCGCCGGAG GGCAACCGAG TAAGCGACGA GGAAGTGCTG ATCCAACAGC TGCTGGAAGA TGCGATCAAA GACCGCGTAC CGAGTGATGA AGACTGCCGC CGGTACTTCG AGCAGAACCC GGAGCGCTTC CGCTCCCCCG ACCGCGTGCG TCTGCGCCAT ATCCTGCTGG CCGCGCCGGC CGACGACGTG GCTGGGCGCT TCGCCGCACG TACCGAGGGC GAGCGGCTGG TCGGGCTGCT GAAAGAGTCG CCGCACCTGT TCGCCGATTT CGCCCTGCGC CATTCGCGTT GCCCATCCAG CAGCGAGGGC GGCGACCTGG GCTGGCTGCA GCGTGGGCAG ACCACGCCGG AGTTCGACCG CCAGGTGTTC CGCCTGCGCG AGGGCCTGGC CGGTTTCCCG GTGGAATCGC GCTGGGGCTA CCACGTGGTC TGCGTCGATG CACGCGAGGA AGGCCAGCCG CAGCCGTTCG AGGCGGTGCT GCCGCAGCTG CGTGACTACC TGGAGCTGCA GGTACGCCAG CGTGAAGTGC AGGCCTACCT GCTGCAGCTG CAGGAACGCT ACCCGGTGCG CGGGCTGGAT GAGATCGAAG CCGAAGCGGA TATCGGC

Details for StmaA.17266.a.B1.GE43383

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG SLPKFLPITV IDSAAPVPAE AHSHHHDAAA QGPRSLGQPA PCRLFVDETA ISEADIAREM QHHRAMRPEQ SRAEAARALV VRELLRLEAQ RLGLQAPEGN RVSDEEVLIQ QLLEDAIKDR VPSDEDCRRY FEQNPERFRS PDRVRLRHIL LAAPADDVAG RFAARTEGER LVGLLKESPH LFADFALRHS RCPSSSEGGD LGWLQRGQTT PEFDRQVFRL REGLAGFPVE SRWGYHVVCV DAREEGQPQP FEAVLPQLRD YLELQVRQRE VQAYLLQLQE RYPVRGLDEI EAEADIG

Validated NT Sequence

tactcnctta gccgatatcc gcttcggctt cgatctcatc cagcccgcgc accgggtagc gttcctgcag ctgcagcagg taggcctgca cttcacgctg gcgtacctgc agctccaggt agtcacgcag ctgcggcagc accgcctcga acggctgcgg ctggccttcc tcgcgtgcat cgacgcagac cacgtggtag ccccagcgcg attccaccgg gaaaccggcc aggccctcgc gcaggcggaa cacctggcgg tcgaactccg gcgtggtctg cccacgctgc agccagccca ggtcgccgcc ctcgctgctg gatgggcaac gcgaatggcg cagggcgaaa tcggcgaaca ggtgcggcga ctctttcagc agcccgacca gccgctcgcc ctcggtacgt gcggcgaagc gcccagccac gtcgtcggcc ggcgcggcca gcaggatatg gcgcagacgc acgcggtcgg gggagcggaa gcgctccggg ttctgctcga agtaccggcg gcagtcttca tcactcggta cgcggtcttt gatcgcatct tccagcagct gttggatcag cacttcctcg tcgcttactc ggttgccctc cggcgcctgc agcccaagcc gctgcgcttc caggcgcagc agctcgcgca ccaccagtgc acgtgccgct tcggcacgag actgctccgg gcgcatcgcc cggtggtgct gcatctcgcg ggcgatgtcg gcctcgctga tcgcggtctc gtccacgaac agccggcacg gtgccggctg cccgagcgag cgcggcccct gcgctgccgc gtcatggtgg tgcgaatgcg cctcggcggg aaccggcgca gcggagtcga tcacggtgat cggcaggaat ttcggcaggc tgcccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca gcctgccgaa attcctgccg atcaccgtga tcgactccgc tgcgccggtt cccgccgagg cgcattcgca ccaccatgac gcggcagcgc aggggccgcg ctcgctcggg cagccggcac cgtgccggct gttcgtggac gagaccgcga tcagcgaggc cgacatcgcc cgcgagatgc agcaccaccg ggcgatgcgc ccggagcagt ctcgtgccga agcggcacgt gcactggtgg tgcgcgagct gctgcgcctg gaagcgcagc ggcttgggct gcaggcgccg gagggcaacc gagtaagcga cgaggaagtg ctgatccaac agctgctgga agatgcgatc aaagaccgcg taccgagtga tgaagactgc cgccggtact tcgagcagaa cccggagcgc ttccgctccc ccgaccgcgt gcgtctgcgc catatcctgc tggccgcgcc ggccgacgac gtggctgggc gcttcgccgc acgtaccgag ggcgagcggc tggtcgggct gctgaaagag tcgccgcacc tgttcgccga tttcgccctg cgccattcgc gttgcccatc cagcagcgag ggcggcgacc tgggctggct gcagcgtggg cagaccacgc cggagttcga ccgccaggtg ttccgcctgc gcgagggcct ggccggtttc ccggtggaat cgcgctgggg ctaccacgtg gtctgcgtcg atgcacgcga ggaaggccag ccgcagccgt tcgaggcggt gctgccgcag ctgcgtgact acctggagct gcaggtacgc cagcgtgaag tgcaggccta cctgctgcag ctgcaggaac gctacccggt gcgcgggctg gatgagatcg aagccgaagc ggatatcggc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat