SSGCID: Target Page

StmaA.10014.k

ABC transporter involved in cytochrome c biogenesis, ATPase component CcmA (ccmA)

CENTER ID:	StmaA.10014.k
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.10014.k.B1.GE43374	Full length(StmaA.10014.k)		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3066

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLAASGLSFS RNDEPVFGPL DFHVDAGEAL LVQGGNGAGK TTLLRVLAGL ARPGAGQVRI DGKPASNAER ARYVAYLSHL PALKPDLDTL ENLHFLCGLH GRRARQMPGN ALAIVGLAGY EDTLVRHLSA GQKRRLALAR IWLSPAPLWL LDEPYANLDL EGITLVNRMI SAHLRAGGAA LVTTHGAYAA PPVRTRQLDL GGAA

NT Sequence

TTGCTGGCCG CCAGCGGCCT GAGCTTCTCC CGCAATGACG AGCCGGTGTT CGGCCCGCTG GACTTCCATG TGGACGCCGG CGAAGCCCTG CTGGTGCAGG GCGGCAATGG CGCCGGCAAG ACCACCCTGC TGCGCGTGCT GGCCGGCCTG GCCCGGCCCG GCGCCGGCCA GGTGCGGATC GACGGCAAGC CGGCGAGCAA TGCCGAACGT GCACGCTACG TGGCCTACCT CAGCCATCTG CCGGCCCTGA AGCCAGACCT GGACACGCTG GAGAACCTGC ACTTCCTGTG CGGCCTGCAT GGACGGCGTG CGCGGCAGAT GCCCGGCAAC GCGCTGGCCA TCGTGGGCCT GGCCGGTTAT GAGGACACCC TCGTTCGGCA TCTGTCCGCC GGGCAGAAGC GGCGGCTGGC ACTGGCACGC ATCTGGCTGT CGCCGGCGCC GCTGTGGCTG CTGGACGAGC CCTATGCCAA CCTCGACCTG GAGGGCATCA CCCTGGTCAA CCGGATGATC TCGGCGCACC TGCGTGCCGG CGGCGCTGCG CTGGTCACCA CCCATGGCGC CTATGCCGCA CCGCCGGTGC GTACCCGCCA GCTCGACCTC GGCGGTGCCG CA

Details for StmaA.10014.k.B1.GE43374

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML LAASGLSFSR NDEPVFGPLD FHVDAGEALL VQGGNGAGKT TLLRVLAGLA RPGAGQVRID GKPASNAERA RYVAYLSHLP ALKPDLDTLE NLHFLCGLHG RRARQMPGNA LAIVGLAGYE DTLVRHLSAG QKRRLALARI WLSPAPLWLL DEPYANLDLE GITLVNRMIS AHLRAGGAAL VTTHGAYAAP PVRTRQLDLG GAA

Validated NT Sequence

atcttactca cttatgcggc accgccgagg tcgagctggc gggtacgcac cggcggtgcg gcataggcgc catgggtggt gaccagcgca gcgccgccgg cacgcaggtg cgccgagatc atccggttga ccagggtgat gccctccagg tcgaggttgg catagggctc gtccagcagc cacagcggcg ccggcgacag ccagatgcgt gccagtgcca gccgccgctt ctgcccggcg gacagatgcc gaacgagggt gtcctcataa ccggccaggc ccacgatggc cagcgcgttg ccgggcatct gccgcgcacg ccgtccatgc aggccgcaca ggaagtgcag gttctccagc gtgtccaggt ctggcttcag ggccggcaga tggctgaggt aggccacgta gcgtgcacgt tcggcattgc tcgccggctt gccgtcgatc cgcacctggc cggcgccggg ccgggccagg ccggccagca cgcgcagcag ggtggtcttg ccggcgccat tgccgccctg caccagcagg gcttcgccgg cgtccacatg gaagtccagc gggccgaaca ccggctcgtc attgcgggag aagctcaggc cgctggcggc cagcaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHML AASGLSFSRN DEPVFGPLDF HVDAGEALLV QGGNGAGKTT LLRVLAGLAR PGAGQVRIDG KPASNAERAR YVAYLSHLPA LKPDLDTLEN LHFLCGLHGR RARQMPGNAL AIVGLAGYED TLVRHLSAGQ KRRLALARIW LSPAPLWLLD EPYANLDLEG ITLVNRMISA HLRAGGAALV TTHGAYAAPP VRTRQLDLGG AA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgctgg ccgccagcgg cctgagcttc tcccgcaatg acgagccggt gttcggcccg ctggacttcc atgtggacgc cggcgaagcc ctgctggtgc agggcggcaa tggcgccggc aagaccaccc tgctgcgcgt gctggccggc ctggcccggc ccggcgccgg ccaggtgcgg atcgacggca agccggcgag caatgccgaa cgtgcacgct acgtggccta cctcagccat ctgccggccc tgaagccaga cctggacacg ctggagaacc tgcacttcct gtgcggcctg catggacggc gtgcgcggca gatgcccggc aacgcgctgg ccatcgtggg cctggccggt tatgaggaca ccctcgttcg gcatctgtcc gccgggcaga agcggcggct ggcactggca cgcatctggc tgtcgccggc gccgctgtgg ctgctggacg agccctatgc caacctcgac ctggagggca tcaccctggt caaccggatg atctcggcgc acctgcgtgc cggcggcgct gcgctggtca ccacccatgg cgcctatgcc gcaccgccgg tgcgtacccg ccagctcgac ctcggcggtg ccgcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat