SSGCID: Target Page

StmaA.10014.e

Phospholipid ABC transporter ATP-binding protein MlaF

CENTER ID:	StmaA.10014.e
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.10014.e.B1.GE43372	Full length(StmaA.10014.e)		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.4403

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSASTSSLVQ LSNVRIDRSG RTILRDVSLQ VPQGSITAVL GPSGSGKSTL LAALTGELRP VAGDVTLFGK PIPHDSSALL ETRKSVGVLL QGNGLLTDLT VAENVALPLR THTRLPAAVL RRLVEMKLHA VGLLAAADAW PRELSGGMAR RVALARALAL DPPLMIYDEP LTGLDPIASG VIMSLIQRLN HSLGLTSIIV SHHVHETLPI CDQVIAIANG GVVFQGTPEA LQSSQDPLLR QFLHGQPDGP IPFDAAPRAR VA

NT Sequence

ATGTCGGCTT CCACCTCCAG TCTGGTGCAG TTGTCCAACG TCCGCATCGA CCGGAGCGGA CGCACGATCC TGCGTGACGT GTCGCTGCAG GTGCCGCAGG GCAGCATCAC CGCCGTGCTC GGACCCTCCG GCAGCGGCAA GTCGACCCTG CTGGCGGCGC TGACCGGCGA ACTGCGACCG GTGGCCGGCG ACGTCACCCT GTTCGGCAAG CCGATCCCGC ATGACAGCAG CGCGCTGCTG GAGACGCGCA AGAGCGTGGG CGTGCTGCTG CAGGGCAATG GTCTGCTGAC CGACCTCACC GTGGCCGAGA ACGTCGCCCT GCCGCTGCGT ACGCATACCC GCCTGCCGGC CGCCGTGCTG CGCCGGCTGG TGGAGATGAA GCTGCATGCG GTGGGCCTGC TGGCCGCCGC CGACGCCTGG CCGCGCGAAC TGTCCGGCGG CATGGCGCGC CGCGTGGCGC TGGCCCGTGC GCTGGCGCTG GACCCACCAC TGATGATCTA CGACGAGCCG CTGACCGGGC TGGACCCGAT CGCCTCCGGG GTGATCATGA GCCTGATCCA GCGCCTCAAC CACAGCCTGG GCCTGACCAG CATCATCGTC AGCCACCACG TGCACGAGAC CCTGCCGATC TGCGACCAGG TGATCGCCAT CGCCAATGGC GGCGTCGTGT TCCAGGGGAC GCCCGAGGCA CTGCAGTCCA GCCAGGATCC GCTGCTGCGG CAGTTCCTGC ATGGCCAGCC GGATGGCCCG ATTCCGTTCG ATGCCGCGCC ACGCGCGAGG GTTGCC

Details for StmaA.10014.e.B1.GE43372

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS ASTSSLVQLS NVRIDRSGRT ILRDVSLQVP QGSITAVLGP SGSGKSTLLA ALTGELRPVA GDVTLFGKPI PHDSSALLET RKSVGVLLQG NGLLTDLTVA ENVALPLRTH TRLPAAVLRR LVEMKLHAVG LLAAADAWPR ELSGGMARRV ALARALALDP PLMIYDEPLT GLDPIASGVI MSLIQRLNHS LGLTSIIVSH HVHETLPICD QVIAIANGGV VFQGTPEALQ SSQDPLLRQF LHGQPDGPIP FDAAPRARVA

Validated NT Sequence

atcttactca cttaggcaac cctcgcgcgt ggcgcggcat cgaacggaat cgggccatcc ggctggccat gcaggaactg ccgcagcagc ggatcctggc tggactgcag tgcctcgggc gtcccctgga acacgacgcc gccattggcg atggcgatca cctggtcgca gatcggcagg gtctcgtgca cgtggtggct gacgatgatg ctggtcaggc ccaggctgtg gttgaggcgc tggatcaggc tcatgatcac cccggaggcg atcgggtcca gcccggtcag cggctcgtcg tagatcatca gtggtgggtc cagcgccagc gcacgggcca gcgccacgcg gcgcgccatg ccgccggaca gttcgcgcgg ccaggcgtcg gcggcggcca gcaggcccac cgcatgcagc ttcatctcca ccagccggcg cagcacggcg gccggcaggc gggtatgcgt acgcagcggc agggcgacgt tctcggccac ggtgaggtcg gtcagcagac cattgccctg cagcagcacg cccacgctct tgcgcgtctc cagcagcgcg ctgctgtcat gcgggatcgg cttgccgaac agggtgacgt cgccggccac cggtcgcagt tcgccggtca gcgccgccag cagggtcgac ttgccgctgc cggagggtcc gagcacggcg gtgatgctgc cctgcggcac ctgcagcgac acgtcacgca ggatcgtgcg tccgctccgg tcgatgcgga cgttggacaa ctgcaccaga ctggaggtgg aagccgacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgg cttccacctc cagtctggtg cagttgtcca acgtccgcat cgaccggagc ggacgcacga tcctgcgtga cgtgtcgctg caggtgccgc agggcagcat caccgccgtg ctcggaccct ccggcagcgg caagtcgacc ctgctggcgg cgctgaccgg cgaactgcga ccggtggccg gcgacgtcac cctgttcggc aagccgatcc cgcatgacag cagcgcgctg ctggagacgc gcaagagcgt gggcgtgctg ctgcagggca atggtctgct gaccgacctc accgtggccg agaacgtcgc cctgccgctg cgtacgcata cccgcctgcc ggccgccgtg ctgcgccggc tggtggagat gaagctgcat gcggtgggcc tgctggccgc cgccgacgcc tggccgcgcg aactgtccgg cggcatggcg cgccgcgtgg cgctggcccg tgcgctggcg ctggacccac cactgatgat ctacgacgag ccgctgaccg ggctggaccc gatcgcctcc ggggtgatca tgagcctgat ccagcgcctc aaccacagcc tgggcctgac cagcatcatc gtcagccacc acgtgcacga gaccctgccg atctgcgacc aggtgatcgc catcgccaat ggcggcgtcg tgttccaggg gacgcccgag gcactgcagt ccagccagga tccgctgctg cggcagttcc tgcatggcca gccggatggc ccgattccgt tcgatgccgc gccacgcgcg agggttgcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat