StmaA.10014.a

Efflux ABC transporter, ATP-binding protein

CENTER ID: StmaA.10014.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.10014.a.B1.GE43368 Full length(StmaA.10014.a) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1532

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHAQGLTRRF GDLLAVDNVD LTVPRGQVYG FLGPNGSGKS TTIRMLCGLL EPSAGQIEVL GLAVPEQAEA LRRRIGYMTQ RFSLYEDLSV RENLEFLAAI QDLPRTQARQ RVDELLQQYR LLDRQPQLAG TLSGGQKQRL ALAGAVVHAP ELLFLDEPTS AVDPESRRDF WEALFELADA GTTVLVSTHY MDEAERCHRL AILDRGALVA DGTPAELCAR LDGRTLQVTS AQPRQASRAL SELPGVLSVA QIGTQLRVLC SEAAADTPAL RRALASADPQ ARIEAVAPNL EDVFVAATRG RGREPAA
NT Sequence
GTGCATGCCC AGGGCCTGAC CCGCCGCTTC GGCGATCTGC TGGCCGTGGA CAATGTCGAC CTGACCGTGC CGCGTGGCCA GGTCTATGGC TTCCTGGGCC CGAACGGGTC GGGCAAGTCG ACCACCATCC GCATGCTGTG CGGCCTGCTG GAACCGAGCG CCGGCCAGAT CGAGGTGCTC GGCCTGGCCG TGCCGGAGCA GGCCGAGGCA CTGCGCCGGC GCATCGGCTA CATGACCCAG CGTTTCTCGT TGTATGAAGA CCTTTCGGTG CGCGAGAACC TGGAATTCCT CGCCGCCATC CAGGACCTGC CGCGTACGCA GGCCCGGCAG CGGGTCGACG AACTGCTGCA GCAGTACCGT CTCCTGGACC GGCAGCCGCA GCTGGCCGGC ACCCTCAGTG GTGGCCAGAA GCAGCGCTTG GCGCTGGCCG GCGCCGTCGT GCACGCGCCC GAGCTGTTGT TCCTGGACGA GCCGACCAGC GCGGTCGACC CGGAGTCACG CCGCGATTTC TGGGAGGCCC TGTTTGAACT TGCCGACGCC GGTACCACCG TGCTGGTATC GACCCACTAC ATGGACGAAG CCGAGCGCTG CCACCGGCTG GCGATCCTCG ATCGAGGCGC GCTGGTCGCC GATGGCACGC CGGCCGAACT GTGTGCACGG CTGGACGGCC GCACGTTGCA GGTCACGTCG GCGCAGCCGC GGCAGGCCAG CCGCGCGTTG TCTGAGCTGC CCGGCGTGCT GAGCGTGGCA CAGATCGGCA CGCAGCTGCG CGTGCTGTGC AGCGAGGCGG CCGCCGATAC GCCAGCGTTG CGGCGCGCGC TGGCCAGCGC CGATCCGCAG GCCCGCATCG AGGCGGTGGC GCCGAACCTG GAAGACGTCT TCGTCGCCGC CACCCGCGGC CGTGGCCGGG AGCCAGCGGC A
Details for StmaA.10014.a.B1.GE43368
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV HAQGLTRRFG DLLAVDNVDL TVPRGQVYGF LGPNGSGKST TIRMLCGLLE PSAGQIEVLG LAVPEQAEAL RRRIGYMTQR FSLYEDLSVR ENLEFLAAIQ DLPRTQARQR VDELLQQYRL LDRQPQLAGT LSGGQKQRLA LAGAVVHAPE LLFLDEPTSA VDPESRRDFW EALFELADAG TTVLVSTHYM DEAERCHRLA ILDRGALVAD GTPAELCARL DGRTLQVTSA QPRQASRALS ELPGVLSVAQ IGTQLRVLCS EAAADTPALR RALASADPQA RIEAVAPNLE DVFVAATXGR GREPAA
Validated NT Sequence
tactcnctta tgccgctggc tcccggccac ggccgnnggt ggcggcgacg aagacgtctt ccaggttcgg cgccaccgcc tcgatgcggg cctgcggatc ggcgctggcc agcgcgcgcc gcaacgctgg cgtatcggcg gccgcctcgc tgcacagcac gcgcagctgc gtgccgatct gtgccacgct cagcacgccg ggcagctcag acaacgcgcg gctggcctgc cgcggctgcg ccgacgtgac ctgcaacgtg cggccgtcca gccgtgcaca cagttcggcc ggcgtgccat cggcgaccag cgcgcctcga tcgaggatcg ccagccggtg gcagcgctcg gcttcgtcca tgtagtgggt cgataccagc acggtggtac cggcgtcggc aagttcaaac agggcctccc agaaatcgcg gcgtgactcc gggtcgaccg cgctggtcgg ctcgtccagg aacaacagct cgggcgcgtg cacgacggcg ccggccagcg ccaagcgctg cttctggcca ccactgaggg tgccggccag ctgcggctgc cggtccagga gacggtactg ctgcagcagt tcgtcgaccc gctgccgggc ctgcgtacgc ggcaggtcct ggatggcggc gaggaattcc aggttctcgc gcaccgaaag gtcttcatac aacgagaaac gctgggtcat gtagccgatg cgccggcgca gtgcctcggc ctgctccggc acggccaggc cgagcacctc gatctggccg gcgctcggtt ccagcaggcc gcacagcatg cggatggtgg tcgacttgcc cgacccgttc gggcccagga agccatagac ctggccacgc ggcacggtca ggtcgacatt gtccacggcc agcagatcgc cgaagcggcg ggtcaggccc tgggcatgca ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMH AQGLTRRFGD LLAVDNVDLT VPRGQVYGFL GPNGSGKSTT IRMLCGLLEP SAGQIEVLGL AVPEQAEALR RRIGYMTQRF SLYEDLSVRE NLEFLAAIQD LPRTQARQRV DELLQQYRLL DRQPQLAGTL SGGQKQRLAL AGAVVHAPEL LFLDEPTSAV DPESRRDFWE ALFELADAGT TVLVSTHYMD EAERCHRLAI LDRGALVADG TPAELCARLD GRTLQVTSAQ PRQASRALSE LPGVLSVAQI GTQLRVLCSE AAADTPALRR ALASADPQAR IEAVAPNLED VFVAATRGRG REPAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcatg cccagggcct gacccgccgc ttcggcgatc tgctggccgt ggacaatgtc gacctgaccg tgccgcgtgg ccaggtctat ggcttcctgg gcccgaacgg gtcgggcaag tcgaccacca tccgcatgct gtgcggcctg ctggaaccga gcgccggcca gatcgaggtg ctcggcctgg ccgtgccgga gcaggccgag gcactgcgcc ggcgcatcgg ctacatgacc cagcgtttct cgttgtatga agacctttcg gtgcgcgaga acctggaatt cctcgccgcc atccaggacc tgccgcgtac gcaggcccgg cagcgggtcg acgaactgct gcagcagtac cgtctcctgg accggcagcc gcagctggcc ggcaccctca gtggtggcca gaagcagcgc ttggcgctgg ccggcgccgt cgtgcacgcg cccgagctgt tgttcctgga cgagccgacc agcgcggtcg acccggagtc acgccgcgat ttctgggagg ccctgtttga acttgccgac gccggtacca ccgtgctggt atcgacccac tacatggacg aagccgagcg ctgccaccgg ctggcgatcc tcgatcgagg cgcgctggtc gccgatggca cgccggccga actgtgtgca cggctggacg gccgcacgtt gcaggtcacg tcggcgcagc cgcggcaggc cagccgcgcg ttgtctgagc tgcccggcgt gctgagcgtg gcacagatcg gcacgcagct gcgcgtgctg tgcagcgagg cggccgccga tacgccagcg ttgcggcgcg cgctggccag cgccgatccg caggcccgca tcgaggcggt ggcgccgaac ctggaagacg tcttcgtcgc cgccacccgc ggccgtggcc gggagccagc ggcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat