SSGCID: Target Page

StmaA.01540.a

Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) (sucB)

CENTER ID:	StmaA.01540.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.01540.a.B1.GE43364	Full length(StmaA.01540.a)		1	400

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.2993

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATEVKAPVL PESVADGTIA TWHKKVGDAV KRDENLLDLE TDKVVLEVPS PVDGVLKEIK FAEGSTVTSS QVVAIIEEGA VAAAPAPAAE AAPAAAAAPA AAAPAAAAAP APAAKSAADA LPPGARFTAI TEGVNPADVD GTGRRGAVTK EDIVNFARNG GAGKAGGARP EERVPMTRIR KRIAERLMES KNSTAMLTTF NEVDLSKVSA ARKELQDEFV KAHGIKLGFM SFFVKAAANA LQRFPLVNAS IDGDDIIYHG YSDISIAVST EKGLVTPVLR NVERMSFADI EKTIADYAKK ARDGKLGLDE LQGGTFTVTN GGTFGSLLST PIINPPQSAI LGMHAIKERP IAQNGQVVIA PMMYLALSYD HRIIDGKDSV QFLVDIKNQL ENPGRMLFGL

NT Sequence

ATGGCCACCG AAGTCAAAGC CCCGGTACTG CCCGAATCCG TCGCCGACGG CACCATCGCC ACCTGGCACA AGAAGGTGGG CGACGCCGTC AAGCGCGACG AAAACCTGCT TGACCTGGAA ACCGACAAGG TCGTCCTGGA AGTGCCGTCG CCGGTCGACG GCGTGCTGAA GGAAATCAAG TTCGCCGAAG GCTCGACCGT GACCTCCAGC CAGGTCGTGG CGATCATCGA AGAAGGCGCC GTGGCTGCTG CCCCGGCTCC GGCTGCCGAA GCCGCTCCGG CTGCAGCCGC TGCCCCGGCC GCTGCTGCTC CGGCCGCTGC CGCCGCTCCG GCCCCGGCCG CCAAGTCGGC CGCCGATGCG CTGCCGCCGG GCGCCCGCTT CACCGCCATC ACCGAAGGCG TGAACCCGGC CGACGTCGAC GGCACCGGCC GTCGCGGCGC GGTGACCAAG GAAGACATCG TCAACTTCGC CCGCAACGGC GGCGCCGGCA AGGCCGGTGG CGCACGTCCG GAAGAACGCG TGCCGATGAC CCGCATCCGC AAGCGCATCG CCGAGCGCCT GATGGAGTCG AAGAACTCCA CCGCGATGCT GACCACCTTC AACGAAGTCG ACCTGTCCAA GGTCTCGGCG GCGCGCAAGG AACTGCAGGA CGAGTTCGTC AAGGCACACG GCATCAAGCT GGGCTTCATG AGCTTCTTCG TGAAGGCCGC CGCCAACGCG CTGCAGCGCT TCCCGCTGGT CAACGCCTCG ATCGACGGCG ACGACATCAT CTACCACGGC TACTCGGACA TCTCGATCGC CGTCTCGACC GAAAAGGGCC TGGTCACCCC GGTCCTGCGC AATGTCGAGC GCATGTCGTT CGCCGACATC GAGAAGACCA TCGCCGACTA CGCCAAGAAG GCGCGTGACG GCAAGCTGGG CCTGGATGAA CTGCAGGGCG GTACCTTCAC CGTGACCAAC GGCGGCACCT TCGGTTCGCT GCTGTCGACC CCGATCATCA ACCCGCCGCA GAGCGCCATC CTGGGCATGC ACGCCATCAA GGAGCGTCCG ATCGCCCAGA ACGGCCAGGT CGTGATCGCG CCGATGATGT ACCTGGCGCT GTCGTACGAC CACCGCATCA TCGACGGCAA GGATTCGGTG CAGTTCCTGG TGGACATCAA GAACCAGCTG GAAAACCCGG GCCGCATGCT GTTCGGCCTG

Details for StmaA.01540.a.B1.GE43364

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA TEVKAPVLPE SVADGTIATW HKKVGDAVKR DENLLDLETD KVVLEVPSPV DGVLKEIKFA EGSTVTSSQV VAIIEEGAVA AAPAPAAEAA PAAAAAPAAA APAAAAAPAP AAKSAADALP PGARFTAITE GVNPADVDGT GRRGAVTKED IVNFARNGGA GKAGGARPEE RVPMTRIRKR IAERLMESKN STAMLTTFNE VDLSKVSAAR KELQDEFVKA HGIKLGFMSF FVKAAANALQ RFPLVNASID GDDIIYHGYS DISIAVSTEK GLVTPVLRNV ERMSFADIEK TIADYAKKAR DGKLGLDELQ GGTFTVTNGG TFGSLLSTPI INPPQSAILG MHAIKERPIA QNGQVVIAPM MYLALSYDHR IIDGKDSVQF LVDIKNQXEX XX

Validated NT Sequence

ggcnnnnntt ttccngctgg ttcttgatgt ccaccaggaa ctgcaccgaa tccttgccgt cgatgatgcg gtggtcgtac gacagcgcca ggtacatcat cggcgcgatc acgacctggc cgttctgggc gatcggacgc tccttgatgg cgtgcatgcc caggatggcg ctctgcggcg ggttgatgat cggggtcgac agcagcgaac cgaaggtgcc gccgttggtc acggtgaagg taccgccctg cagttcatcc aggcccagct tgccgtcacg cgccttcttg gcgtagtcgg cgatggtctt ctcgatgtcg gcgaacgaca tgcgctcgac attgcgcagg accggggtga ccaggccctt ttcggtcgag acggcgatcg agatgtccga gtagccgtgg tagatgatgt cgtcgccgtc gatcgaggcg ttgaccagcg ggaagcgctg cagcgcgttg gcggcggcct tcacgaagaa gctcatgaag cccagcttga tgccgtgtgc cttgacgaac tcgtcctgca gttccttgcg cgccgccgag accttggaca ggtcgacttc gttgaaggtg gtcagcatcg cggtggagtt cttcgactcc atcaggcgct cggcgatgcg cttgcggatg cgggtcatcg gcacgcgttc ttccggacgt gcgccaccgg ccttgccggc gccgccgttg cgggcgaagt tgacgatgtc ttccttggtc accgcgccgc gacggccggt gccgtcgacg tcggccgggt tcacgccttc ggtgatggcg gtgaagcggg cgcccggcgg cagcgcatcg gcggccgact tggcggccgg ggccggagcg gcggcagcgg ccggagcagc agcggccggg gcagcggctg cagccggagc ggcttcggca gccggagccg gggcagcagc cacggcgcct tcttcgatga tcgccacgac ctggctggag gtcacggtcg agccttcggc gaacttgatt tccttcagca cgccgtcgac cggcgacggc acttccagga cgaccttgtc ggtttccagg tcaagcaggt tttcgtcgcg cttgacggcg tcgcccacct tcttgtgcca ggtggcgatg gtgccgtcgg cgacggattc gggcagtacc ggggctttga cttcggtggc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca ccgaagtcaa agccccggta ctgcccgaat ccgtcgccga cggcaccatc gccacctggc acaagaaggt gggcgacgcc gtcaagcgcg acgaaaacct gcttgacctg gaaaccgaca aggtcgtcct ggaagtgccg tcgccggtcg acggcgtgct gaaggaaatc aagttcgccg aaggctcgac cgtgacctcc agccaggtcg tggcgatcat cgaagaaggc gccgtggctg ctgccccggc tccggctgcc gaagccgctc cggctgcagc cgctgccccg gccgctgctg ctccggccgc tgccgccgct ccggccccgg ccgccaagtc ggccgccgat gcgctgccgc cgggcgcccg cttcaccgcc atcaccgaag gcgtgaaccc ggccgacgtc gacggcaccg gccgtcgcgg cgcggtgacc aaggaagaca tcgtcaactt cgcccgcaac ggcggcgccg gcaaggccgg tggcgcacgt ccggaagaac gcgtgccgat gacccgcatc cgcaagcgca tcgccgagcg cctgatggag tcgaagaact ccaccgcgat gctgaccacc ttcaacgaag tcgacctgtc caaggtctcg gcggcgcgca aggaactgca ggacgagttc gtcaaggcac acggcatcaa gctgggcttc atgagcttct tcgtgaaggc cgccgccaac gcgctgcagc gcttcccgct ggtcaacgcc tcgatcgacg gcgacgacat catctaccac ggctactcgg acatctcgat cgccgtctcg accgaaaagg gcctggtcac cccggtcctg cgcaatgtcg agcgcatgtc gttcgccgac atcgagaaga ccatcgccga ctacgccaag aaggcgcgtg acggcaagct gggcctggat gaactgcagg gcggtacctt caccgtgacc aacggcggca ccttcggttc gctgctgtcg accccgatca tcaacccgcc gcagagcgcc atcctgggca tgcacgccat caaggagcgt ccgatcgccc agaacggcca ggtcgtgatc gcgccgatga tgtacctggc gctgtcgtac gaccaccgca tcatcgacgg caaggattcg gtgcagttcc tggtggacat caagaaccag ctggaaaacc cgggccgcat gctgttcggc ctgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat