StmaA.01536.a
Pyridoxine 5'-phosphate synthase (EC 2.6.99.2)
| CENTER ID: | StmaA.01536.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01536.a.B1.GE43363 | Full length(StmaA.01536.a) | 1 | 247 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01536.a.B1.PW38756 | Full length(StmaA.01536.a) | 1 | 247 |
Structures
6W6A
DEPOSITED: 3/16/2020
DETERMINATION: XRay
CLONE: StmaA.01536.a.B1.GE43363
PROTEIN: StmaA.01536.a.B1.PW38756
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|522373.3.peg.15 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQLSVNVNK IAVLRNSRGG AEPDVVRAAQ ACLDAGAHGI TVHPRPDRRH ITAEDVLALS TLTRARGVEF NIEGNPFAPP REGYPGLLPL CAQTRPAQAT LVPDGDGQIT SDHGFDFERD AERLRPLVAE LKAMGCRVSL FVDAGNPLLE QAAEVGADRV ELYTGPYAEA HAAGDAAAML ELFATAARRA QAMGLGVNAG HDLSQDNLRD FLAHVPDVLE VSIGHALIGE ALYDGLDATV RGYLALL
NT Sequence
ATGACCCAGC TCAGCGTCAA CGTCAACAAG ATCGCCGTCC TGCGCAATTC GCGCGGCGGC GCCGAACCGG ACGTGGTGCG CGCCGCCCAG GCCTGCCTGG ATGCCGGTGC ACATGGCATC ACCGTGCACC CGCGGCCGGA CCGCCGCCAT ATCACCGCCG AGGACGTGCT GGCGCTGTCC ACGCTGACCC GCGCGCGTGG TGTCGAGTTC AACATCGAAG GCAACCCGTT CGCACCGCCG CGCGAGGGCT ACCCAGGCCT GCTGCCGCTG TGCGCGCAGA CCCGCCCGGC GCAGGCGACG CTGGTGCCGG ATGGCGATGG ACAGATCACC TCCGACCATG GCTTCGACTT CGAGCGGGAT GCCGAGCGGC TGCGACCACT GGTCGCCGAA CTGAAGGCGA TGGGCTGCCG GGTCAGCCTG TTCGTCGATG CTGGCAACCC ACTGCTGGAG CAGGCGGCCG AAGTCGGTGC AGATCGTGTC GAGCTGTATA CCGGCCCCTA CGCCGAAGCA CACGCGGCCG GCGACGCTGC GGCGATGCTG GAACTGTTCG CCACTGCGGC GCGGCGGGCG CAGGCGATGG GCCTGGGCGT CAACGCCGGG CACGACCTGT CACAGGACAA CCTACGCGAT TTCCTGGCCC ACGTGCCGGA CGTGCTGGAG GTGTCGATCG GTCACGCGCT GATCGGTGAG GCGCTGTACG ACGGCTTGGA TGCCACCGTG CGCGGGTACC TGGCGTTGCT C
Details for StmaA.01536.a.B1.GE43363
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT QLSVNVNKIA VLRNSRGGAE PDVVRAAQAC LDAGAHGITV HPRPDRRHIT AEDVLALSTL TRARGVEFNI EGNPFAPPRE GYPGLLPLCA QTRPAQATLV PDGDGQITSD HGFDFERDAE RLRPLVAELK AMGCRVSLFV DAGNPLLEQA AEVGADRVEL YTGPYAEAHA AGDAAAMLEL FATAARRAQA MGLGVNAGHD LSQDNLRDFL AHVPDVLEVS IGHALIGEAL YDGLDATVRG YLALL
Validated NT Sequence
atcttactca cttagagcaa cgccaggtac ccgcgcacgg tggcatccaa gccgtcgtac agcgcctcac cgatcagcgc gtgaccgatc gacacctcca gcacgtccgg cacgtgggcc aggaaatcgc gtaggttgtc ctgtgacagg tcgtgcccgg cgttgacgcc caggcccatc gcctgcgccc gccgcgccgc agtggcgaac agttccagca tcgccgcagc gtcgccggcc gcgtgtgctt cggcgtaggg gccggtatac agctcgacac gatctgcacc gacttcggcc gcctgctcca gcagtgggtt gccagcatcg acgaacaggc tgacccggca gcccatcgcc ttcagttcgg cgaccagtgg tcgcagccgc tcggcatccc gctcgaagtc gaagccatgg tcggaggtga tctgtccatc gccatccggc accagcgtcg cctgcgccgg gcgggtctgc gcgcacagcg gcagcaggcc tgggtagccc tcgcgcggcg gtgcgaacgg gttgccttcg atgttgaact cgacaccacg cgcgcgggtc agcgtggaca gcgccagcac gtcctcggcg gtgatatggc ggcggtccgg ccgcgggtgc acggtgatgc catgtgcacc ggcatccagg caggcctggg cggcgcgcac cacgtccggt tcggcgccgc cgcgcgaatt gcgcaggacg gcgatcttgt tgacgttgac gctgagctgg gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT QLSVNVNKIA VLRNSRGGAE PDVVRAAQAC LDAGAHGITV HPRPDRRHIT AEDVLALSTL TRARGVEFNI EGNPFAPPRE GYPGLLPLCA QTRPAQATLV PDGDGQITSD HGFDFERDAE RLRPLVAELK AMGCRVSLFV DAGNPLLEQA AEVGADRVEL YTGPYAEAHA AGDAAAMLEL FATAARRAQA MGLGVNAGHD LSQDNLRDFL AHVPDVLEVS IGHALIGEAL YDGLDATVRG YLALL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc agctcagcgt caacgtcaac aagatcgccg tcctgcgcaa ttcgcgcggc ggcgccgaac cggacgtggt gcgcgccgcc caggcctgcc tggatgccgg tgcacatggc atcaccgtgc acccgcggcc ggaccgccgc catatcaccg ccgaggacgt gctggcgctg tccacgctga cccgcgcgcg tggtgtcgag ttcaacatcg aaggcaaccc gttcgcaccg ccgcgcgagg gctacccagg cctgctgccg ctgtgcgcgc agacccgccc ggcgcaggcg acgctggtgc cggatggcga tggacagatc acctccgacc atggcttcga cttcgagcgg gatgccgagc ggctgcgacc actggtcgcc gaactgaagg cgatgggctg ccgggtcagc ctgttcgtcg atgctggcaa cccactgctg gagcaggcgg ccgaagtcgg tgcagatcgt gtcgagctgt ataccggccc ctacgccgaa gcacacgcgg ccggcgacgc tgcggcgatg ctggaactgt tcgccactgc ggcgcggcgg gcgcaggcga tgggcctggg cgtcaacgcc gggcacgacc tgtcacagga caacctacgc gatttcctgg cccacgtgcc ggacgtgctg gaggtgtcga tcggtcacgc gctgatcggt gaggcgctgt acgacggctt ggatgccacc gtgcgcgggt acctggcgtt gctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for StmaA.01536.a.B1.PW38756
| PURIFICATION DATe: | 11/12/2019 |
| CONCENTRATION: | 11.4mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QLSVNVNKIA VLRNSRGGAE PDVVRAAQAC LDAGAHGITV HPRPDRRHIT AEDVLALSTL TRARGVEFNI EGNPFAPPRE GYPGLLPLCA QTRPAQATLV PDGDGQITSD HGFDFERDAE RLRPLVAELK AMGCRVSLFV DAGNPLLEQA AEVGADRVEL YTGPYAEAHA AGDAAAMLEL FATAARRAQA MGLGVNAGHD LSQDNLRDFL AHVPDVLEVS IGHALIGEAL YDGLDATVRG YLALL
Validated NT Sequence
atcttactca cttagagcaa cgccaggtac ccgcgcacgg tggcatccaa gccgtcgtac agcgcctcac cgatcagcgc gtgaccgatc gacacctcca gcacgtccgg cacgtgggcc aggaaatcgc gtaggttgtc ctgtgacagg tcgtgcccgg cgttgacgcc caggcccatc gcctgcgccc gccgcgccgc agtggcgaac agttccagca tcgccgcagc gtcgccggcc gcgtgtgctt cggcgtaggg gccggtatac agctcgacac gatctgcacc gacttcggcc gcctgctcca gcagtgggtt gccagcatcg acgaacaggc tgacccggca gcccatcgcc ttcagttcgg cgaccagtgg tcgcagccgc tcggcatccc gctcgaagtc gaagccatgg tcggaggtga tctgtccatc gccatccggc accagcgtcg cctgcgccgg gcgggtctgc gcgcacagcg gcagcaggcc tgggtagccc tcgcgcggcg gtgcgaacgg gttgccttcg atgttgaact cgacaccacg cgcgcgggtc agcgtggaca gcgccagcac gtcctcggcg gtgatatggc ggcggtccgg ccgcgggtgc acggtgatgc catgtgcacc ggcatccagg caggcctggg cggcgcgcac cacgtccggt tcggcgccgc cgcgcgaatt gcgcaggacg gcgatcttgt tgacgttgac gctgagctgg gtcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMT QLSVNVNKIA VLRNSRGGAE PDVVRAAQAC LDAGAHGITV HPRPDRRHIT AEDVLALSTL TRARGVEFNI EGNPFAPPRE GYPGLLPLCA QTRPAQATLV PDGDGQITSD HGFDFERDAE RLRPLVAELK AMGCRVSLFV DAGNPLLEQA AEVGADRVEL YTGPYAEAHA AGDAAAMLEL FATAARRAQA MGLGVNAGHD LSQDNLRDFL AHVPDVLEVS IGHALIGEAL YDGLDATVRG YLALL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AGCTCAGCGT CAACGTCAAC AAGATCGCCG TCCTGCGCAA TTCGCGCGGC GGCGCCGAAC CGGACGTGGT GCGCGCCGCC CAGGCCTGCC TGGATGCCGG TGCACATGGC ATCACCGTGC ACCCGCGGCC GGACCGCCGC CATATCACCG CCGAGGACGT GCTGGCGCTG TCCACGCTGA CCCGCGCGCG TGGTGTCGAG TTCAACATCG AAGGCAACCC GTTCGCACCG CCGCGCGAGG GCTACCCAGG CCTGCTGCCG CTGTGCGCGC AGACCCGCCC GGCGCAGGCG ACGCTGGTGC CGGATGGCGA TGGACAGATC ACCTCCGACC ATGGCTTCGA CTTCGAGCGG GATGCCGAGC GGCTGCGACC ACTGGTCGCC GAACTGAAGG CGATGGGCTG CCGGGTCAGC CTGTTCGTCG ATGCTGGCAA CCCACTGCTG GAGCAGGCGG CCGAAGTCGG TGCAGATCGT GTCGAGCTGT ATACCGGCCC CTACGCCGAA GCACACGCGG CCGGCGACGC TGCGGCGATG CTGGAACTGT TCGCCACTGC GGCGCGGCGG GCGCAGGCGA TGGGCCTGGG CGTCAACGCC GGGCACGACC TGTCACAGGA CAACCTACGC GATTTCCTGG CCCACGTGCC GGACGTGCTG GAGGTGTCGA TCGGTCACGC GCTGATCGGT GAGGCGCTGT ACGACGGCTT GGATGCCACC GTGCGCGGGT ACCTGGCGTT GCTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT