StmaA.01469.a

Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)

CENTER ID: StmaA.01469.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01469.a.B1.GE43362 Full length(StmaA.01469.a) 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.4109

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQTKNFNLP DLGEGLPDAT IVEWFVKEGD VIKLDEPLVS METAKAVVEV PSPFSGKVLK LSGGAGDIIP TGSVLASFEL DPNLPQRADG QDTGHSHGHA APAAPAAAPT PPPLPAAAAP VAAEEAKPAA AERDDAGTVV GAMQSSNAVH AEQALAVGGV KAVPAVRAMA RKLGVDLSRV AATGTDGAVT MADVKQAAAN GTAKLGAAPA PVAAAAYAAP APAQVSAPVQ SEARTPLSAA GKPMRTQPPG VVAKGQPEPL KGVRRNMARV MADAHSKVVP TTLNDDADIH AWLPGNDVTA RLVRSIVVAA QKVPAMNAWF DGEALTRTLH AQVDIGIAVD TDDGLFVPAL RNADMLDARG IREGVNRLRE QVESRSIAAS ELSGYTISLS NFGMFAGRYA TPVVVPPCVA IVGAGRARHQ MTPVMGGVEA HKVIPLSVTF DHRAATGGEA ARFLRAMMDD LALAS
NT Sequence
ATGAGCCAGA CCAAGAACTT CAACCTGCCC GACCTGGGCG AAGGCCTGCC GGACGCGACC ATCGTCGAGT GGTTCGTCAA GGAAGGCGAT GTGATCAAGC TGGACGAGCC GCTGGTGTCG ATGGAGACCG CCAAGGCCGT GGTGGAAGTG CCCTCGCCGT TCTCCGGCAA GGTGCTGAAG CTGTCCGGCG GTGCCGGCGA CATCATCCCG ACCGGTTCGG TGCTGGCCAG CTTCGAACTG GACCCGAACC TGCCGCAGCG TGCCGATGGC CAGGACACCG GCCACAGCCA TGGTCATGCC GCACCGGCCG CGCCGGCTGC CGCACCGACC CCGCCGCCGC TTCCCGCTGC TGCCGCACCG GTGGCCGCAG AAGAAGCCAA GCCCGCCGCT GCCGAGCGTG ATGACGCCGG TACCGTGGTC GGTGCGATGC AGAGCTCCAA TGCCGTGCAT GCCGAACAGG CGCTGGCCGT CGGTGGCGTC AAGGCCGTGC CGGCCGTGCG TGCGATGGCG CGCAAGCTGG GCGTGGACCT GAGCCGCGTG GCCGCTACCG GCACCGATGG CGCGGTCACC ATGGCCGACG TCAAGCAGGC TGCCGCCAAC GGCACCGCCA AGCTCGGCGC TGCTCCGGCA CCGGTTGCCG CTGCTGCCTA TGCCGCGCCG GCCCCGGCGC AGGTGTCTGC CCCGGTGCAG AGCGAAGCCC GCACCCCGCT GTCCGCCGCC GGCAAGCCGA TGCGCACCCA GCCGCCGGGC GTGGTCGCCA AGGGCCAGCC GGAACCGCTG AAGGGCGTGC GCCGCAACAT GGCCCGCGTG ATGGCCGATG CGCACAGCAA GGTGGTGCCG ACCACGCTGA ACGACGACGC CGACATCCAT GCCTGGCTGC CGGGCAACGA CGTCACCGCG CGCCTGGTGC GTTCGATCGT GGTCGCTGCA CAGAAGGTGC CGGCGATGAA CGCCTGGTTC GACGGTGAAG CGCTGACCCG TACCCTGCAC GCGCAGGTGG ACATCGGCAT CGCCGTGGAC ACCGACGACG GCCTGTTCGT GCCGGCCCTG CGCAATGCCG ACATGCTGGA TGCACGTGGC ATCCGCGAAG GCGTCAACCG CCTGCGCGAG CAGGTGGAAT CGCGTTCGAT CGCCGCCTCG GAACTGAGCG GCTACACCAT CTCGCTGTCC AACTTCGGCA TGTTCGCCGG CCGCTACGCG ACCCCGGTCG TGGTGCCGCC GTGCGTGGCC ATCGTCGGTG CCGGCCGTGC CCGCCACCAG ATGACCCCGG TGATGGGCGG CGTGGAAGCG CACAAGGTGA TCCCGCTGTC GGTCACCTTC GACCACCGCG CGGCCACCGG CGGCGAAGCG GCGCGCTTCC TGCGCGCAAT GATGGACGAC CTGGCGCTGG CCAGC
Details for StmaA.01469.a.B1.GE43362
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 69
Validated AA Sequence
MAHHHHHHHM SQTKNFNLPD LGEGLPDATI VEWFVKEGDV IKLDEPLVSM ETAKAVVEVP SPFSGKVLKL SGGAGDIIPT GSVLASFELD PNLPQRADGQ DTGHSHGHAA PAAPAAAPTP PPLPAAAAPV AAEEAKPAAA ERDDAGTVVG AMQSSNAVHA EQALAVGGVK AVPAVRAMAR KLGVDLSRVA ATGTDGAVTM ADVKQAAANG TAKLGAAPAP VAAAAYAAPA PAQVSAPVQS EARTPLSAAG KPMRTQPPGV VAKGQPEPLK GVRRNMARVX GRCAQQGGAD HAERRRRHPC LAAGQRRHRA PGAFDRGRCT EGAGDERLVR R
Validated NT Sequence
ttgcgcgcag gaagcgcgcc gcttcgccgc cggtggccgc gcggtggtcg aaggtgaccg acagcgggat caccttgtgc gcttccacgc cgcccatcac cggggtcatc tggtggcggg cacggccggc accgacgatg gccacgcacg gcggcaccac gaccggggtc gcgtagcggc cggcgaacat gccgaagttg gacagcgaga tggtgtagcc gctcagttcc gaggcggcga tcgaacgcga ttccacctgc tcgcgcaggc ggttgacgcc ttcgcggatg ccacgtgcat ccagcatgtc ggcattgcgc agggccggca cgaacaggcc gtcgtcggtg tccacggcga tgccgatgtc cacctgcgcg tgcagggtac gggtcagcgc ttcaccgtcg aaccaggcgt tcatcgccgg caccttctgt gcagcgacca cgatcgaacg caccaggcgc gcggtgacgt cgttgcccgg cagccaggca tggatgtcgg cgtcgtcgtt cagcgtggtc ggcaccacct tgctgtgcgc atcggccnat cacgcgggcc atgttgcggc gcacgccctt cagcggttcc ggctggccct tggcgaccac gcccggcggc tgggtgcgca tcggcttgcc ggcggcggac agcggggtgc gggcttcgct ctgcaccggg gcagacacct gcgccggggc cggcgcggca taggcagcag cggcaaccgg tgccggagca gcgccgagct tggcggtgcc gttggcggca gcctgcttga cgtcggccat ggtgaccgcg ccatcggtgc cggtagcggc cacgcggctc aggtccacgc ccagcttgcg cgccatcgca cgcacggccg gcacggcctt gacgccaccg acggccagcg cctgttcggc atgcacggca ttggagctct gcatcgcacc gaccacggta ccggcgtcat cacgctcggc agcggcgggc ttggcttctt ctgcggccac cggtgcggca gcagcgggaa gcggcggcgg ggtcggtgcg gcagccggcg cggccggtgc ggcatgacca tggctgtggc cggtgtcctg gccatcggca cgctgcggca ggttcgggtc cagttcgaag ctggccagca ccgaaccggt cgggatgatg tcgccggcac cgccggacag cttcagcacc ttgccggaga acggcgaggg cacttccacc acggccttgg cggtctccat cgacaccagc ggctcgtcca gcttgatcac atcgccttcc ttgacgaacc actcgacgat ggtcgcgtcc ggcaggcctt cgcccaggtc gggcaggttg aagttcttgg tctggctcat atggtggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS QTKNFNLPDL GEGLPDATIV EWFVKEGDVI KLDEPLVSME TAKAVVEVPS PFSGKVLKLS GGAGDIIPTG SVLASFELDP NLPQRADGQD TGHSHGHAAP AAPAAAPTPP PLPAAAAPVA AEEAKPAAAE RDDAGTVVGA MQSSNAVHAE QALAVGGVKA VPAVRAMARK LGVDLSRVAA TGTDGAVTMA DVKQAAANGT AKLGAAPAPV AAAAYAAPAP AQVSAPVQSE ARTPLSAAGK PMRTQPPGVV AKGQPEPLKG VRRNMARVMA DAHSKVVPTT LNDDADIHAW LPGNDVTARL VRSIVVAAQK VPAMNAWFDG EALTRTLHAQ VDIGIAVDTD DGLFVPALRN ADMLDARGIR EGVNRLREQV ESRSIAASEL SGYTISLSNF GMFAGRYATP VVVPPCVAIV GAGRARHQMT PVMGGVEAHK VIPLSVTFDH RAATGGEAAR FLRAMMDDLA LAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc agaccaagaa cttcaacctg cccgacctgg gcgaaggcct gccggacgcg accatcgtcg agtggttcgt caaggaaggc gatgtgatca agctggacga gccgctggtg tcgatggaga ccgccaaggc cgtggtggaa gtgccctcgc cgttctccgg caaggtgctg aagctgtccg gcggtgccgg cgacatcatc ccgaccggtt cggtgctggc cagcttcgaa ctggacccga acctgccgca gcgtgccgat ggccaggaca ccggccacag ccatggtcat gccgcaccgg ccgcgccggc tgccgcaccg accccgccgc cgcttcccgc tgctgccgca ccggtggccg cagaagaagc caagcccgcc gctgccgagc gtgatgacgc cggtaccgtg gtcggtgcga tgcagagctc caatgccgtg catgccgaac aggcgctggc cgtcggtggc gtcaaggccg tgccggccgt gcgtgcgatg gcgcgcaagc tgggcgtgga cctgagccgc gtggccgcta ccggcaccga tggcgcggtc accatggccg acgtcaagca ggctgccgcc aacggcaccg ccaagctcgg cgctgctccg gcaccggttg ccgctgctgc ctatgccgcg ccggccccgg cgcaggtgtc tgccccggtg cagagcgaag cccgcacccc gctgtccgcc gccggcaagc cgatgcgcac ccagccgccg ggcgtggtcg ccaagggcca gccggaaccg ctgaagggcg tgcgccgcaa catggcccgc gtgatggccg atgcgcacag caaggtggtg ccgaccacgc tgaacgacga cgccgacatc catgcctggc tgccgggcaa cgacgtcacc gcgcgcctgg tgcgttcgat cgtggtcgct gcacagaagg tgccggcgat gaacgcctgg ttcgacggtg aagcgctgac ccgtaccctg cacgcgcagg tggacatcgg catcgccgtg gacaccgacg acggcctgtt cgtgccggcc ctgcgcaatg ccgacatgct ggatgcacgt ggcatccgcg aaggcgtcaa ccgcctgcgc gagcaggtgg aatcgcgttc gatcgccgcc tcggaactga gcggctacac catctcgctg tccaacttcg gcatgttcgc cggccgctac gcgaccccgg tcgtggtgcc gccgtgcgtg gccatcgtcg gtgccggccg tgcccgccac cagatgaccc cggtgatggg cggcgtggaa gcgcacaagg tgatcccgct gtcggtcacc ttcgaccacc gcgcggccac cggcggcgaa gcggcgcgct tcctgcgcgc aatgatggac gacctggcgc tggccagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat