StmaA.01463.a
Guanylate kinase (EC 2.7.4.8) (gmk)
| CENTER ID: | StmaA.01463.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01463.a.B1.GE43361 | Full length(StmaA.01463.a) | 1 | 221 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01463.a.B1.PW38755 | Full length(StmaA.01463.a) | 1 | 221 |
Structures
6WCT
DEPOSITED: 3/31/2020
DETERMINATION: XRay
CLONE: StmaA.01463.a.B1.GE43361
PROTEIN: StmaA.01463.a.B1.PW38755
7S5E
DEPOSITED: 9/10/2021
DETERMINATION: XRay
CLONE: StmaA.01463.a.B1.GE43361
PROTEIN: StmaA.01463.a.B1.PW38755
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3619 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPSKPSDA VARGTLYIVA APSGAGKSSI VNATLARDPQ IALSISFTSR AMRPGEVNGQ HYHFVSAEKF EQMIAAGDFF EHAWVHGDWK GTARQSVEPQ LAAGQDVLLE IDWQGAQQVR QLVPGTVTVF ILPPSKQALQ DRMRKRGQDS EAVIAQRLGA ARDEMLHFNE FDYVIVNEVF DTAVDELCAI FTASRLRREA QKVRHAGLIQ ALLTPDPGAT D
NT Sequence
ATGAGCGCCC CGTCGAAGCC GTCGGACGCC GTTGCGCGCG GCACGCTCTA CATCGTTGCC GCCCCGTCCG GTGCCGGCAA GAGCAGCATC GTCAATGCCA CCCTGGCGCG TGACCCGCAG ATCGCCCTGT CGATCTCCTT CACCTCGCGC GCGATGCGCC CGGGTGAAGT GAACGGCCAG CACTACCATT TCGTCTCTGC GGAGAAGTTC GAGCAAATGA TCGCCGCCGG CGACTTCTTC GAACACGCCT GGGTGCACGG CGACTGGAAG GGCACTGCCC GCCAGTCGGT GGAGCCGCAG CTGGCCGCCG GCCAGGACGT ACTGCTGGAG ATCGACTGGC AGGGCGCGCA GCAGGTGCGC CAGCTGGTGC CGGGCACGGT TACCGTGTTC ATCCTGCCGC CATCCAAGCA GGCCCTGCAG GACCGCATGC GCAAGCGCGG CCAGGACAGC GAGGCGGTCA TCGCCCAGCG CCTGGGCGCG GCCCGCGATG AAATGCTGCA CTTCAACGAG TTCGACTACG TCATCGTCAA CGAGGTGTTC GACACTGCGG TGGACGAGCT GTGCGCCATC TTCACCGCCA GCCGCCTGCG CCGGGAGGCC CAGAAGGTCC GCCATGCCGG CCTGATCCAG GCCCTGCTGA CCCCCGATCC GGGTGCAACT GAC
Details for StmaA.01463.a.B1.GE43361
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS APSKPSDAVA RGTLYIVAAP SGAGKSSIVN ATLARDPQIA LSISFTSRAM RPGEVNGQHY HFVSAEKFEQ MIAAGDFFEH AWVHGDWKGT ARQSVEPQLA AGQDVLLEID WQGAQQVRQL VPGTVTVFIL PPSKQALQDR MRKRGQDSEA VIAQRLGAAR DEMLHFNEFD YVIVNEVFDT AVDELCAIFT ASRLRREAQK VRHAGLIQAL LTPDPGATD
Validated NT Sequence
atcttactca cttagtcagt tgcacccgga tcgggggtca gcagggcctg gatcaggccg gcatggcgga ccttctgggc ctcccggcgc aggcggctgg cggtgaagat ggcgcacagc tcgtccaccg cagtgtcgaa cacctcgttg acgatgacgt agtcgaactc gttgaagtgc agcatttcat cgcgggccgc gcccaggcgc tgggcgatga ccgcctcgct gtcctggccg cgcttgcgca tgcggtcctg cagggcctgc ttggatggcg gcaggatgaa cacggtaacc gtgcccggca ccagctggcg cacctgctgc gcgccctgcc agtcgatctc cagcagtacg tcctggccgg cggccagctg cggctccacc gactggcggg cagtgccctt ccagtcgccg tgcacccagg cgtgttcgaa gaagtcgccg gcggcgatca tttgctcgaa cttctccgca gagacgaaat ggtagtgctg gccgttcact tcacccgggc gcatcgcgcg cgaggtgaag gagatcgaca gggcgatctg cgggtcacgc gccagggtgg cattgacgat gctgctcttg ccggcaccgg acggggcggc aacgatgtag agcgtgccgc gcgcaacggc gtccgacggc ttcgacgggg cgctcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS APSKPSDAVA RGTLYIVAAP SGAGKSSIVN ATLARDPQIA LSISFTSRAM RPGEVNGQHY HFVSAEKFEQ MIAAGDFFEH AWVHGDWKGT ARQSVEPQLA AGQDVLLEID WQGAQQVRQL VPGTVTVFIL PPSKQALQDR MRKRGQDSEA VIAQRLGAAR DEMLHFNEFD YVIVNEVFDT AVDELCAIFT ASRLRREAQK VRHAGLIQAL LTPDPGATD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ccccgtcgaa gccgtcggac gccgttgcgc gcggcacgct ctacatcgtt gccgccccgt ccggtgccgg caagagcagc atcgtcaatg ccaccctggc gcgtgacccg cagatcgccc tgtcgatctc cttcacctcg cgcgcgatgc gcccgggtga agtgaacggc cagcactacc atttcgtctc tgcggagaag ttcgagcaaa tgatcgccgc cggcgacttc ttcgaacacg cctgggtgca cggcgactgg aagggcactg cccgccagtc ggtggagccg cagctggccg ccggccagga cgtactgctg gagatcgact ggcagggcgc gcagcaggtg cgccagctgg tgccgggcac ggttaccgtg ttcatcctgc cgccatccaa gcaggccctg caggaccgca tgcgcaagcg cggccaggac agcgaggcgg tcatcgccca gcgcctgggc gcggcccgcg atgaaatgct gcacttcaac gagttcgact acgtcatcgt caacgaggtg ttcgacactg cggtggacga gctgtgcgcc atcttcaccg ccagccgcct gcgccgggag gcccagaagg tccgccatgc cggcctgatc caggccctgc tgacccccga tccgggtgca actgactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for StmaA.01463.a.B1.PW38755
| PURIFICATION DATe: | 11/12/2019 |
| CONCENTRATION: | 33mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS APSKPSDAVA RGTLYIVAAP SGAGKSSIVN ATLARDPQIA LSISFTSRAM RPGEVNGQHY HFVSAEKFEQ MIAAGDFFEH AWVHGDWKGT ARQSVEPQLA AGQDVLLEID WQGAQQVRQL VPGTVTVFIL PPSKQALQDR MRKRGQDSEA VIAQRLGAAR DEMLHFNEFD YVIVNEVFDT AVDELCAIFT ASRLRREAQK VRHAGLIQAL LTPDPGATD
Validated NT Sequence
atcttactca cttagtcagt tgcacccgga tcgggggtca gcagggcctg gatcaggccg gcatggcgga ccttctgggc ctcccggcgc aggcggctgg cggtgaagat ggcgcacagc tcgtccaccg cagtgtcgaa cacctcgttg acgatgacgt agtcgaactc gttgaagtgc agcatttcat cgcgggccgc gcccaggcgc tgggcgatga ccgcctcgct gtcctggccg cgcttgcgca tgcggtcctg cagggcctgc ttggatggcg gcaggatgaa cacggtaacc gtgcccggca ccagctggcg cacctgctgc gcgccctgcc agtcgatctc cagcagtacg tcctggccgg cggccagctg cggctccacc gactggcggg cagtgccctt ccagtcgccg tgcacccagg cgtgttcgaa gaagtcgccg gcggcgatca tttgctcgaa cttctccgca gagacgaaat ggtagtgctg gccgttcact tcacccgggc gcatcgcgcg cgaggtgaag gagatcgaca gggcgatctg cgggtcacgc gccagggtgg cattgacgat gctgctcttg ccggcaccgg acggggcggc aacgatgtag agcgtgccgc gcgcaacggc gtccgacggc ttcgacgggg cgctcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMS APSKPSDAVA RGTLYIVAAP SGAGKSSIVN ATLARDPQIA LSISFTSRAM RPGEVNGQHY HFVSAEKFEQ MIAAGDFFEH AWVHGDWKGT ARQSVEPQLA AGQDVLLEID WQGAQQVRQL VPGTVTVFIL PPSKQALQDR MRKRGQDSEA VIAQRLGAAR DEMLHFNEFD YVIVNEVFDT AVDELCAIFT ASRLRREAQK VRHAGLIQAL LTPDPGATD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CCCCGTCGAA GCCGTCGGAC GCCGTTGCGC GCGGCACGCT CTACATCGTT GCCGCCCCGT CCGGTGCCGG CAAGAGCAGC ATCGTCAATG CCACCCTGGC GCGTGACCCG CAGATCGCCC TGTCGATCTC CTTCACCTCG CGCGCGATGC GCCCGGGTGA AGTGAACGGC CAGCACTACC ATTTCGTCTC TGCGGAGAAG TTCGAGCAAA TGATCGCCGC CGGCGACTTC TTCGAACACG CCTGGGTGCA CGGCGACTGG AAGGGCACTG CCCGCCAGTC GGTGGAGCCG CAGCTGGCCG CCGGCCAGGA CGTACTGCTG GAGATCGACT GGCAGGGCGC GCAGCAGGTG CGCCAGCTGG TGCCGGGCAC GGTTACCGTG TTCATCCTGC CGCCATCCAA GCAGGCCCTG CAGGACCGCA TGCGCAAGCG CGGCCAGGAC AGCGAGGCGG TCATCGCCCA GCGCCTGGGC GCGGCCCGCG ATGAAATGCT GCACTTCAAC GAGTTCGACT ACGTCATCGT CAACGAGGTG TTCGACACTG CGGTGGACGA GCTGTGCGCC ATCTTCACCG CCAGCCGCCT GCGCCGGGAG GCCCAGAAGG TCCGCCATGC CGGCCTGATC CAGGCCCTGC TGACCCCCGA TCCGGGTGCA ACTGACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T