StmaA.01413.a

Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) (purN)

CENTER ID: StmaA.01413.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01413.a.B1.GE43359 Full length(StmaA.01413.a) 1 219
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01413.a.B1.PW38754 Full length(StmaA.01413.a) 1 219

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1086

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAPTRIAVL ASGRGSNLQA ILDAIGSGRL SAAVVGVFSD RPAAEALLRV DAGLRWAHAP KEFSDRASYE QALGDALAAV QPDWIVCAGY MRILGPAFVQ RFDGRLVNIH PSLLPLHKGL DTHARALQAG DAEHGASVHL VVPELDAGAV LAQARVPVRP GDDAQALAAR VLAVEHPLLI ATLQLLCEGR LAEREGQPWL DGHPLFSPLA LDSAGNLNR
NT Sequence
ATGACCGCCC CAACCCGCAT CGCCGTGCTC GCCTCGGGCC GCGGCAGCAA CCTGCAGGCC ATCCTCGACG CGATCGGCAG CGGCCGCCTG TCGGCCGCGG TGGTCGGCGT GTTCTCCGAC CGCCCCGCCG CAGAGGCCCT GCTGCGTGTC GATGCCGGCC TGCGCTGGGC GCATGCACCG AAGGAATTCA GCGACCGCGC CAGCTACGAG CAGGCGCTCG GCGATGCGCT TGCCGCGGTA CAGCCGGACT GGATCGTCTG TGCCGGCTAC ATGCGCATCC TTGGTCCGGC CTTCGTGCAG CGTTTCGATG GCCGCCTGGT CAACATCCAC CCGTCGCTGC TGCCGCTGCA CAAGGGCCTG GACACCCACG CGCGGGCGCT GCAGGCCGGT GACGCCGAGC ATGGCGCCAG CGTGCACCTG GTGGTCCCCG AACTGGATGC CGGCGCGGTG CTGGCGCAGG CCCGCGTGCC GGTGCGGCCC GGCGATGACG CGCAGGCGCT GGCCGCACGC GTGCTTGCGG TGGAGCATCC GCTGCTGATC GCCACACTGC AGTTGCTGTG CGAGGGCCGC CTGGCTGAAC GGGAGGGTCA GCCCTGGCTG GACGGTCACC CCCTGTTTAG CCCCTTGGCC CTAGATTCCG CCGGAAACCT GAACCGG
Details for StmaA.01413.a.B1.GE43359
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT APTRIAVLAS GRGSNLQAIL DAIGSGRLSA AVVGVFSDRP AAEALLRVDA GLRWAHAPKE FSDRASYEQA LGDALAAVQP DWIVCAGYMR ILGPAFVQRF DGRLVNIHPS LLPLHKGLDT HARALQAGDA EHGASVHLVV PELDAGAVLA QARVPVRPGD DAQALAARVL AVEHPLLIAT LQLLCEGRLA EREGQPWLDG HPLFSPLALD SAGNLNR
Validated NT Sequence
atcttactca cttaccggtt caggtttccg gcggaatcta gggccaaggg gctaaacagg gggtgaccgt ccagccaggg ctgaccctcc cgttcagcca ggcggccctc gcacagcaac tgcagtgtgg cgatcagcag cggatgctcc accgcaagca cgcgtgcggc cagcgcctgc gcgtcatcgc cgggccgcac cggcacgcgg gcctgcgcca gcaccgcgcc ggcatccagt tcggggacca ccaggtgcac gctggcgcca tgctcggcgt caccggcctg cagcgcccgc gcgtgggtgt ccaggccctt gtgcagcggc agcagcgacg ggtggatgtt gaccaggcgg ccatcgaaac gctgcacgaa ggccggacca aggatgcgca tgtagccggc acagacgatc cagtccggct gtaccgcggc aagcgcatcg ccgagcgcct gctcgtagct ggcgcggtcg ctgaattcct tcggtgcatg cgcccagcgc aggccggcat cgacacgcag cagggcctct gcggcggggc ggtcggagaa cacgccgacc accgcggccg acaggcggcc gctgccgatc gcgtcgagga tggcctgcag gttgctgccg cggcccgagg cgagcacggc gatgcgggtt ggggcggtca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMT APTRIAVLAS GRGSNLQAIL DAIGSGRLSA AVVGVFSDRP AAEALLRVDA GLRWAHAPKE FSDRASYEQA LGDALAAVQP DWIVCAGYMR ILGPAFVQRF DGRLVNIHPS LLPLHKGLDT HARALQAGDA EHGASVHLVV PELDAGAVLA QARVPVRPGD DAQALAARVL AVEHPLLIAT LQLLCEGRLA EREGQPWLDG HPLFSPLALD SAGNLNR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ccccaacccg catcgccgtg ctcgcctcgg gccgcggcag caacctgcag gccatcctcg acgcgatcgg cagcggccgc ctgtcggccg cggtggtcgg cgtgttctcc gaccgccccg ccgcagaggc cctgctgcgt gtcgatgccg gcctgcgctg ggcgcatgca ccgaaggaat tcagcgaccg cgccagctac gagcaggcgc tcggcgatgc gcttgccgcg gtacagccgg actggatcgt ctgtgccggc tacatgcgca tccttggtcc ggccttcgtg cagcgtttcg atggccgcct ggtcaacatc cacccgtcgc tgctgccgct gcacaagggc ctggacaccc acgcgcgggc gctgcaggcc ggtgacgccg agcatggcgc cagcgtgcac ctggtggtcc ccgaactgga tgccggcgcg gtgctggcgc aggcccgcgt gccggtgcgg cccggcgatg acgcgcaggc gctggccgca cgcgtgcttg cggtggagca tccgctgctg atcgccacac tgcagttgct gtgcgagggc cgcctggctg aacgggaggg tcagccctgg ctggacggtc accccctgtt tagccccttg gccctagatt ccgccggaaa cctgaaccgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for StmaA.01413.a.B1.PW38754
PURIFICATION DATe: 11/10/2019
CONCENTRATION: 43mg/ml
OBSERVED MW: 24kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT APTRIAVLAS GRGSNLQAIL DAIGSGRLSA AVVGVFSDRP AAEALLRVDA GLRWAHAPKE FSDRASYEQA LGDALAAVQP DWIVCAGYMR ILGPAFVQRF DGRLVNIHPS LLPLHKGLDT HARALQAGDA EHGASVHLVV PELDAGAVLA QARVPVRPGD DAQALAARVL AVEHPLLIAT LQLLCEGRLA EREGQPWLDG HPLFSPLALD SAGNLNR
Validated NT Sequence
atcttactca cttaccggtt caggtttccg gcggaatcta gggccaaggg gctaaacagg gggtgaccgt ccagccaggg ctgaccctcc cgttcagcca ggcggccctc gcacagcaac tgcagtgtgg cgatcagcag cggatgctcc accgcaagca cgcgtgcggc cagcgcctgc gcgtcatcgc cgggccgcac cggcacgcgg gcctgcgcca gcaccgcgcc ggcatccagt tcggggacca ccaggtgcac gctggcgcca tgctcggcgt caccggcctg cagcgcccgc gcgtgggtgt ccaggccctt gtgcagcggc agcagcgacg ggtggatgtt gaccaggcgg ccatcgaaac gctgcacgaa ggccggacca aggatgcgca tgtagccggc acagacgatc cagtccggct gtaccgcggc aagcgcatcg ccgagcgcct gctcgtagct ggcgcggtcg ctgaattcct tcggtgcatg cgcccagcgc aggccggcat cgacacgcag cagggcctct gcggcggggc ggtcggagaa cacgccgacc accgcggccg acaggcggcc gctgccgatc gcgtcgagga tggcctgcag gttgctgccg cggcccgagg cgagcacggc gatgcgggtt ggggcggtca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMT APTRIAVLAS GRGSNLQAIL DAIGSGRLSA AVVGVFSDRP AAEALLRVDA GLRWAHAPKE FSDRASYEQA LGDALAAVQP DWIVCAGYMR ILGPAFVQRF DGRLVNIHPS LLPLHKGLDT HARALQAGDA EHGASVHLVV PELDAGAVLA QARVPVRPGD DAQALAARVL AVEHPLLIAT LQLLCEGRLA EREGQPWLDG HPLFSPLALD SAGNLNR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG CCCCAACCCG CATCGCCGTG CTCGCCTCGG GCCGCGGCAG CAACCTGCAG GCCATCCTCG ACGCGATCGG CAGCGGCCGC CTGTCGGCCG CGGTGGTCGG CGTGTTCTCC GACCGCCCCG CCGCAGAGGC CCTGCTGCGT GTCGATGCCG GCCTGCGCTG GGCGCATGCA CCGAAGGAAT TCAGCGACCG CGCCAGCTAC GAGCAGGCGC TCGGCGATGC GCTTGCCGCG GTACAGCCGG ACTGGATCGT CTGTGCCGGC TACATGCGCA TCCTTGGTCC GGCCTTCGTG CAGCGTTTCG ATGGCCGCCT GGTCAACATC CACCCGTCGC TGCTGCCGCT GCACAAGGGC CTGGACACCC ACGCGCGGGC GCTGCAGGCC GGTGACGCCG AGCATGGCGC CAGCGTGCAC CTGGTGGTCC CCGAACTGGA TGCCGGCGCG GTGCTGGCGC AGGCCCGCGT GCCGGTGCGG CCCGGCGATG ACGCGCAGGC GCTGGCCGCA CGCGTGCTTG CGGTGGAGCA TCCGCTGCTG ATCGCCACAC TGCAGTTGCT GTGCGAGGGC CGCCTGGCTG AACGGGAGGG TCAGCCCTGG CTGGACGGTC ACCCCCTGTT TAGCCCCTTG GCCCTAGATT CCGCCGGAAA CCTGAACCGG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT