StmaA.01370.a

Phosphoglucosamine mutase (EC 5.4.2.10)

CENTER ID: StmaA.01370.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01370.a.B1.GE43358 Full length(StmaA.01370.a) 1 453
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01370.a.B1.PW38767 Full length(StmaA.01370.a) 1 453

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3202

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGRKYFGTD GIRGRVGQGV ISADFVLRLG NALGRVLVAQ RGQDGRRPIV VIGKDTRISG YMFEAALEAG LVAAGADVQL LGPMPTPAVA FLTRTLGVDA GIVISASHNP HYDNGIKFFS AQGEKLDDAT ELALEAALDV PFTTAESEKL GKASRAREAV GRYIEFCKAS VPRAFDLRGV RLVLDCAHGA TYQIAPLLFR ELGAEVIGIG AEPNGVNINA GVGSTHIDNL AAKVRETRAD LGIAFDGDGD RVLMADDQGN PVDGDDLLYI LARAWKDEGR LRGPVVGTLM SNYGVEKAFA DLQIPFVRSN VGDRYVHQAL VEGGGVLGGE ASGHLLCLDR ATTGDGIVSA LQVLVALRNG GQTLRQALKG LERVPQKTVN VRLGNVSAKA TVQADSVQAA LAAAQQSVAG RGRAFLRPSG TEPVVRVTVE ADDATLMQET LDRLSAAVRE AAA
NT Sequence
ATGGGAGGCC GCAAGTACTT CGGTACTGAC GGCATCCGTG GGCGGGTCGG CCAAGGCGTG ATCTCGGCCG ACTTCGTGCT GCGCCTGGGC AACGCCCTGG GCCGTGTCCT GGTCGCCCAG CGCGGCCAGG ACGGGCGCCG GCCGATCGTG GTGATCGGCA AGGACACCCG CATTTCCGGC TACATGTTCG AAGCGGCGCT GGAGGCCGGC CTGGTCGCTG CCGGCGCCGA CGTACAGCTG CTGGGGCCGA TGCCGACCCC GGCGGTGGCG TTCCTGACCC GCACGCTGGG CGTGGACGCC GGCATCGTCA TCAGCGCTTC GCACAACCCG CATTACGACA ACGGCATCAA GTTCTTCTCC GCCCAGGGCG AGAAGCTCGA CGACGCCACC GAACTGGCCC TGGAAGCGGC GCTGGACGTT CCGTTCACCA CCGCCGAATC GGAGAAGCTG GGCAAGGCGT CGCGCGCGCG CGAAGCGGTC GGCCGCTACA TCGAGTTCTG CAAGGCCAGC GTGCCGCGCG CGTTCGACCT GCGTGGCGTG CGCCTGGTGC TGGATTGCGC GCACGGTGCC ACCTACCAGA TCGCACCGCT GCTGTTCCGC GAACTGGGCG CCGAGGTGAT CGGCATCGGT GCCGAGCCCA ATGGCGTCAA CATCAACGCA GGCGTGGGTT CCACCCATAT CGACAACCTG GCGGCCAAAG TCCGCGAGAC CCGTGCCGAT CTCGGCATCG CCTTCGATGG TGATGGCGAC CGTGTGCTGA TGGCCGACGA CCAGGGCAAC CCGGTCGACG GTGACGACCT GCTGTACATC CTGGCCCGCG CCTGGAAGGA CGAAGGCCGC CTGCGCGGCC CGGTGGTCGG CACGCTGATG AGCAACTACG GCGTGGAGAA AGCTTTCGCC GATCTGCAGA TCCCGTTCGT GCGCAGCAAC GTTGGTGATC GATACGTGCA CCAGGCGCTG GTCGAAGGCG GGGGCGTGCT TGGCGGTGAG GCCTCGGGCC ACCTGCTGTG CCTGGACCGG GCGACCACCG GCGACGGTAT TGTCAGTGCC CTGCAGGTAC TGGTGGCACT GCGCAACGGC GGCCAGACCC TGCGCCAGGC GCTGAAGGGT TTGGAGCGGG TGCCGCAGAA AACGGTCAAC GTGCGGTTGG GCAATGTCTC GGCCAAGGCC ACGGTGCAGG CCGACAGTGT GCAGGCCGCG CTGGCGGCCG CGCAGCAGTC GGTGGCCGGC CGCGGTCGCG CGTTCCTGCG CCCGTCGGGT ACCGAGCCGG TGGTGCGGGT TACCGTCGAA GCCGATGACG CCACGCTGAT GCAGGAGACA CTCGATCGCT TGTCCGCTGC GGTGCGCGAA GCCGCTGCC
Details for StmaA.01370.a.B1.GE43358
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 78
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMG GRKYFGTDGI RGRVGQGVIS ADFVLRLGNA LGRVLVAQRG QDGRRPIVVI GKDTRISGYM FEAALEAGLV AAGADVQLLG PMPTPAVAFL TRTLGVDAGI VISASHNPHY DNGIKFFSAQ GEKLDDATEL ALEAALDVPF TTAESEKLGK ASRAREAVGP LHRVLQGQRA ARVRPAWRAP GAGLRARCHL PDRTAAVPRT GRRGDRHRCR AQWRQHQRRR GFHPYRQPGG QSPRDPCRSR HRLRW
Validated NT Sequence
acnagcgatc gagtgtctcc tgcatcagcg tggcgtcatc ggcttcgacg gtaacccgca ccaccggctc ggtacccgac gggcgcagga acgcgcgacc gcggccggcc accgactgct gcgcggccgc cagcgcggcc tgcacactgt cggcctgcac cgtggccttg gccgagacat tgcccaaccg cacgttgacc gttttctgcg gcacccgctc caaacccttc agcgcctggc gcagggtctg gccgccgttg cgcagtgcca ccagtacctg cagggcactg acaataccgt cgccggtggt cgcccggtcc aggcacagca ggtggcccga ggcctcaccg ccaagcacgc ccccgccttc gaccagcgcc tggtgcacgt atcgatcacc aacgttgctg cgcacgaacg ggatctgcag atcggcgaaa gctttctcca cgccgtagtt gctcatcagc gtgccgacca ccgggccgcg caggcggcct tcgtccttcc aggcgcgggc caggatgtac agcaggtcgt caccgtcgac cgggttgccc tggtcgtcgg ccatcagcac acggtcgcca tcaccatcga aggcgatgcc gagatcggca cgggtctcgc ggactttggc cgccaggttg tcgatatggg tggaacccac gcctgcgttg atgttgacgc cattgggctc ggcaccgatg ccgatcacct cggcgcccag ttcgcggaac agcagcggtg cgatctggta ggtggcaccg tgcgcgcaat ccagcaccag gcgcacgcca cgcaggtcga acgcgcgcgg cacgctggcc ttgcagaact cgatgtagcg gnccgaccgc ttcgcgcgcg cgcgacgcct tgcccagctt ctccgattcg gcggtggtga acggaacgtc cagcgccgct tccagggcca gttcggtggc gtcgtcgagc ttctcgccct gggcggagaa gaacttgatg ccgttgtcgt aatgcgggtt gtgcgaagcg ctgatgacga tgccggcgtc cacgcccagc gtgcgggtca ggaacgccac cgccggggtc ggcatcggcc ccagcagctg tacgtcggcg ccggcagcga ccaggccggc ctccagcgcc gcttcgaaca tgtagccgga aatgcgggtg tccttgccga tcaccacgat cggccggcgc ccgtcctggc cgcgctgggc gaccaggaca cggcccaggg cgttgcccag gcgcagcacg aagtcggccg agatcacgcc ttggccgacc cgcccacgga tgccgtcagt accgaagtac ttgcggcctc ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMG GRKYFGTDGI RGRVGQGVIS ADFVLRLGNA LGRVLVAQRG QDGRRPIVVI GKDTRISGYM FEAALEAGLV AAGADVQLLG PMPTPAVAFL TRTLGVDAGI VISASHNPHY DNGIKFFSAQ GEKLDDATEL ALEAALDVPF TTAESEKLGK ASRAREAVGR YIEFCKASVP RAFDLRGVRL VLDCAHGATY QIAPLLFREL GAEVIGIGAE PNGVNINAGV GSTHIDNLAA KVRETRADLG IAFDGDGDRV LMADDQGNPV DGDDLLYILA RAWKDEGRLR GPVVGTLMSN YGVEKAFADL QIPFVRSNVG DRYVHQALVE GGGVLGGEAS GHLLCLDRAT TGDGIVSALQ VLVALRNGGQ TLRQALKGLE RVPQKTVNVR LGNVSAKATV QADSVQAALA AAQQSVAGRG RAFLRPSGTE PVVRVTVEAD DATLMQETLD RLSAAVREAA A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggag gccgcaagta cttcggtact gacggcatcc gtgggcgggt cggccaaggc gtgatctcgg ccgacttcgt gctgcgcctg ggcaacgccc tgggccgtgt cctggtcgcc cagcgcggcc aggacgggcg ccggccgatc gtggtgatcg gcaaggacac ccgcatttcc ggctacatgt tcgaagcggc gctggaggcc ggcctggtcg ctgccggcgc cgacgtacag ctgctggggc cgatgccgac cccggcggtg gcgttcctga cccgcacgct gggcgtggac gccggcatcg tcatcagcgc ttcgcacaac ccgcattacg acaacggcat caagttcttc tccgcccagg gcgagaagct cgacgacgcc accgaactgg ccctggaagc ggcgctggac gttccgttca ccaccgccga atcggagaag ctgggcaagg cgtcgcgcgc gcgcgaagcg gtcggccgct acatcgagtt ctgcaaggcc agcgtgccgc gcgcgttcga cctgcgtggc gtgcgcctgg tgctggattg cgcgcacggt gccacctacc agatcgcacc gctgctgttc cgcgaactgg gcgccgaggt gatcggcatc ggtgccgagc ccaatggcgt caacatcaac gcaggcgtgg gttccaccca tatcgacaac ctggcggcca aagtccgcga gacccgtgcc gatctcggca tcgccttcga tggtgatggc gaccgtgtgc tgatggccga cgaccagggc aacccggtcg acggtgacga cctgctgtac atcctggccc gcgcctggaa ggacgaaggc cgcctgcgcg gcccggtggt cggcacgctg atgagcaact acggcgtgga gaaagctttc gccgatctgc agatcccgtt cgtgcgcagc aacgttggtg atcgatacgt gcaccaggcg ctggtcgaag gcgggggcgt gcttggcggt gaggcctcgg gccacctgct gtgcctggac cgggcgacca ccggcgacgg tattgtcagt gccctgcagg tactggtggc actgcgcaac ggcggccaga ccctgcgcca ggcgctgaag ggtttggagc gggtgccgca gaaaacggtc aacgtgcggt tgggcaatgt ctcggccaag gccacggtgc aggccgacag tgtgcaggcc gcgctggcgg ccgcgcagca gtcggtggcc ggccgcggtc gcgcgttcct gcgcccgtcg ggtaccgagc cggtggtgcg ggttaccgtc gaagccgatg acgccacgct gatgcaggag acactcgatc gcttgtccgc tgcggtgcgc gaagccgctg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for StmaA.01370.a.B1.PW38767
PURIFICATION DATe: 12/6/2019
CONCENTRATION: 5mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 78
PERCENT COVERAGE: 55
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG GRKYFGTDGI RGRVGQGVIS ADFVLRLGNA LGRVLVAQRG QDGRRPIVVI GKDTRISGYM FEAALEAGLV AAGADVQLLG PMPTPAVAFL TRTLGVDAGI VISASHNPHY DNGIKFFSAQ GEKLDDATEL ALEAALDVPF TTAESEKLGK ASRAREAVGP LHRVLQGQRA ARVRPAWRAP GAGLRARCHL PDRTAAVPRT GRRGDRHRCR AQWRQHQRRR GFHPYRQPGG QSPRDPCRSR HRLRW
Validated NT Sequence
acnagcgatc gagtgtctcc tgcatcagcg tggcgtcatc ggcttcgacg gtaacccgca ccaccggctc ggtacccgac gggcgcagga acgcgcgacc gcggccggcc accgactgct gcgcggccgc cagcgcggcc tgcacactgt cggcctgcac cgtggccttg gccgagacat tgcccaaccg cacgttgacc gttttctgcg gcacccgctc caaacccttc agcgcctggc gcagggtctg gccgccgttg cgcagtgcca ccagtacctg cagggcactg acaataccgt cgccggtggt cgcccggtcc aggcacagca ggtggcccga ggcctcaccg ccaagcacgc ccccgccttc gaccagcgcc tggtgcacgt atcgatcacc aacgttgctg cgcacgaacg ggatctgcag atcggcgaaa gctttctcca cgccgtagtt gctcatcagc gtgccgacca ccgggccgcg caggcggcct tcgtccttcc aggcgcgggc caggatgtac agcaggtcgt caccgtcgac cgggttgccc tggtcgtcgg ccatcagcac acggtcgcca tcaccatcga aggcgatgcc gagatcggca cgggtctcgc ggactttggc cgccaggttg tcgatatggg tggaacccac gcctgcgttg atgttgacgc cattgggctc ggcaccgatg ccgatcacct cggcgcccag ttcgcggaac agcagcggtg cgatctggta ggtggcaccg tgcgcgcaat ccagcaccag gcgcacgcca cgcaggtcga acgcgcgcgg cacgctggcc ttgcagaact cgatgtagcg gnccgaccgc ttcgcgcgcg cgcgacgcct tgcccagctt ctccgattcg gcggtggtga acggaacgtc cagcgccgct tccagggcca gttcggtggc gtcgtcgagc ttctcgccct gggcggagaa gaacttgatg ccgttgtcgt aatgcgggtt gtgcgaagcg ctgatgacga tgccggcgtc cacgcccagc gtgcgggtca ggaacgccac cgccggggtc ggcatcggcc ccagcagctg tacgtcggcg ccggcagcga ccaggccggc ctccagcgcc gcttcgaaca tgtagccgga aatgcgggtg tccttgccga tcaccacgat cggccggcgc ccgtcctggc cgcgctgggc gaccaggaca cggcccaggg cgttgcccag gcgcagcacg aagtcggccg agatcacgcc ttggccgacc cgcccacgga tgccgtcagt accgaagtac ttgcggcctc ccatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMG GRKYFGTDGI RGRVGQGVIS ADFVLRLGNA LGRVLVAQRG QDGRRPIVVI GKDTRISGYM FEAALEAGLV AAGADVQLLG PMPTPAVAFL TRTLGVDAGI VISASHNPHY DNGIKFFSAQ GEKLDDATEL ALEAALDVPF TTAESEKLGK ASRAREAVGR YIEFCKASVP RAFDLRGVRL VLDCAHGATY QIAPLLFREL GAEVIGIGAE PNGVNINAGV GSTHIDNLAA KVRETRADLG IAFDGDGDRV LMADDQGNPV DGDDLLYILA RAWKDEGRLR GPVVGTLMSN YGVEKAFADL QIPFVRSNVG DRYVHQALVE GGGVLGGEAS GHLLCLDRAT TGDGIVSALQ VLVALRNGGQ TLRQALKGLE RVPQKTVNVR LGNVSAKATV QADSVQAALA AAQQSVAGRG RAFLRPSGTE PVVRVTVEAD DATLMQETLD RLSAAVREAA A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGAG GCCGCAAGTA CTTCGGTACT GACGGCATCC GTGGGCGGGT CGGCCAAGGC GTGATCTCGG CCGACTTCGT GCTGCGCCTG GGCAACGCCC TGGGCCGTGT CCTGGTCGCC CAGCGCGGCC AGGACGGGCG CCGGCCGATC GTGGTGATCG GCAAGGACAC CCGCATTTCC GGCTACATGT TCGAAGCGGC GCTGGAGGCC GGCCTGGTCG CTGCCGGCGC CGACGTACAG CTGCTGGGGC CGATGCCGAC CCCGGCGGTG GCGTTCCTGA CCCGCACGCT GGGCGTGGAC GCCGGCATCG TCATCAGCGC TTCGCACAAC CCGCATTACG ACAACGGCAT CAAGTTCTTC TCCGCCCAGG GCGAGAAGCT CGACGACGCC ACCGAACTGG CCCTGGAAGC GGCGCTGGAC GTTCCGTTCA CCACCGCCGA ATCGGAGAAG CTGGGCAAGG CGTCGCGCGC GCGCGAAGCG GTCGGCCGCT ACATCGAGTT CTGCAAGGCC AGCGTGCCGC GCGCGTTCGA CCTGCGTGGC GTGCGCCTGG TGCTGGATTG CGCGCACGGT GCCACCTACC AGATCGCACC GCTGCTGTTC CGCGAACTGG GCGCCGAGGT GATCGGCATC GGTGCCGAGC CCAATGGCGT CAACATCAAC GCAGGCGTGG GTTCCACCCA TATCGACAAC CTGGCGGCCA AAGTCCGCGA GACCCGTGCC GATCTCGGCA TCGCCTTCGA TGGTGATGGC GACCGTGTGC TGATGGCCGA CGACCAGGGC AACCCGGTCG ACGGTGACGA CCTGCTGTAC ATCCTGGCCC GCGCCTGGAA GGACGAAGGC CGCCTGCGCG GCCCGGTGGT CGGCACGCTG ATGAGCAACT ACGGCGTGGA GAAAGCTTTC GCCGATCTGC AGATCCCGTT CGTGCGCAGC AACGTTGGTG ATCGATACGT GCACCAGGCG CTGGTCGAAG GCGGGGGCGT GCTTGGCGGT GAGGCCTCGG GCCACCTGCT GTGCCTGGAC CGGGCGACCA CCGGCGACGG TATTGTCAGT GCCCTGCAGG TACTGGTGGC ACTGCGCAAC GGCGGCCAGA CCCTGCGCCA GGCGCTGAAG GGTTTGGAGC GGGTGCCGCA GAAAACGGTC AACGTGCGGT TGGGCAATGT CTCGGCCAAG GCCACGGTGC AGGCCGACAG TGTGCAGGCC GCGCTGGCGG CCGCGCAGCA GTCGGTGGCC GGCCGCGGTC GCGCGTTCCT GCGCCCGTCG GGTACCGAGC CGGTGGTGCG GGTTACCGTC GAAGCCGATG ACGCCACGCT GATGCAGGAG ACACTCGATC GCTTGTCCGC TGCGGTGCGC GAAGCCGCTG CCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT