StmaA.01291.a

Adenylate kinase (EC 2.7.4.3) (adk)

CENTER ID: StmaA.01291.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01291.a.B1.GE43297 Full length(StmaA.01291.a) 1 154
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3661

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRAEIAAGT ELGKQAKTVM DAGNLVSDDI LLGMLESRLT QPDVAKGFIL DGYPRNVAQA NAMDGLLAKI GQPLDAVVQL DVATELLVER IAGRAKEQGR ADDTPEAVRQ RLQVYNDQTA PVVDFYAGRG TLARVDGVGE LDEIEARILA AIKA
NT Sequence
ATGCTGCGCG CGGAAATCGC CGCGGGCACG GAGCTGGGCA AGCAGGCCAA GACGGTGATG GATGCCGGCA ACCTGGTGTC GGATGACATC CTGCTGGGCA TGCTGGAGTC GCGCCTGACC CAGCCCGATG TCGCCAAGGG CTTCATTCTT GATGGCTACC CGCGCAATGT GGCCCAGGCC AATGCGATGG ACGGCCTGCT GGCCAAGATC GGCCAGCCGC TGGATGCCGT GGTGCAGCTG GACGTGGCCA CCGAGCTGCT GGTCGAGCGC ATCGCCGGTC GTGCCAAGGA ACAGGGCCGC GCCGACGACA CGCCGGAAGC GGTGCGCCAG CGCCTGCAGG TCTACAACGA CCAGACCGCC CCGGTGGTCG ACTTCTACGC CGGCCGTGGC ACCCTGGCCC GCGTCGATGG CGTCGGTGAG CTGGACGAGA TCGAAGCCCG CATCCTGGCG GCGATCAAGG CC
Details for StmaA.01291.a.B1.GE43297
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 17kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHML RAEIAAGTEL GKQAKTVMDA GNLVSDDILL GMLESRLTQP DVAKGFILDG YPRNVAQANA MDGLLAKIGQ PLDAVVQLDV ATELLVERIA GRAKEQGRAD DTPEAVRQRL QVYNDQTAPV VDFYAGRGTL ARVDGVGELD EIEARILAAI KALSE
Validated NT Sequence
cnnnnnttcg ggctttgtta gcagccggat cctatcttac tcacttaagg ccttgatcgc cgccaggatg cgggcttcga tctcgtccag ctcaccgacg ccatcgacgc gggccagggt gccacggccg gcgtagaagt cgaccaccgg ggcggtctgg tcgttgtaga cctgcaggcg ctggcgcacc gcttccggcg tgtcgtcggc gcggccctgt tccttggcac gaccggcgat gcgctcgacc agcagctcgg tggccacgtc cagctgcacc acggcatcca gcggctggcc gatcttggcc agcaggccgt ccatcgcatt ggcctgggcc acattgcgcg ggtagccatc aagaatgaag cccttggcga catcgggctg ggtcaggcgc gactccagca tgcccagcag gatgtcatcc gacaccaggt tgccggcatc catcaccgtc ttggcctgct tgcccagctc cgtgcccgcg gcgatttccg cgcgcagcat atggtggtgg tggtggtgag ccatggtata tctccttctt aaagttaaac aaaattattt ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata anggganagc gtcnaccgat gcccttgaga gccttcaccc agtcagctnc nttccggtgg gcgcngnatg actancgtcg ccgccttatg actgtctnct tttatcatgc antcgtaagn naggngccgg cgnnnntggt cattttcgnn naggaccnct ttcnntggan nnngannang atnnnngnnn nntnnngnat nnnanntnnn nnnccnnncn ntcanncntc tcnnngnncn nnnccnaann nttntnnnna
Expected Protein Sequence
MAHHHHHHML RAEIAAGTEL GKQAKTVMDA GNLVSDDILL GMLESRLTQP DVAKGFILDG YPRNVAQANA MDGLLAKIGQ PLDAVVQLDV ATELLVERIA GRAKEQGRAD DTPEAVRQRL QVYNDQTAPV VDFYAGRGTL ARVDGVGELD EIEARILAAI KA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcgcggaaat cgccgcgggc acggagctgg gcaagcaggc caagacggtg atggatgccg gcaacctggt gtcggatgac atcctgctgg gcatgctgga gtcgcgcctg acccagcccg atgtcgccaa gggcttcatt cttgatggct acccgcgcaa tgtggcccag gccaatgcga tggacggcct gctggccaag atcggccagc cgctggatgc cgtggtgcag ctggacgtgg ccaccgagct gctggtcgag cgcatcgccg gtcgtgccaa ggaacagggc cgcgccgacg acacgccgga agcggtgcgc cagcgcctgc aggtctacaa cgaccagacc gccccggtgg tcgacttcta cgccggccgt ggcaccctgg cccgcgtcga tggcgtcggt gagctggacg agatcgaagc ccgcatcctg gcggcgatca aggcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat