StmaA.01271.b

GTP-binding protein TypA/BipA (typA)

CENTER ID: StmaA.01271.b
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01271.b.B1.GE43296 Full length(StmaA.01271.b) 1 615
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01271.b.B1.PW38773 Full length(StmaA.01271.b) 1 615

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.906

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIENLRNIA IVAHVDHGKT TLVDQLLKQS GTLSERTVLA ERVMDSNDQE KERGITILAK NTAITWEDKK TGIKNRINIV DTPGHADFGG EVERVLSMVD TVLILVDAMD GPMPQTRFVT QKAFAMGFKP IVVVNKVDRP GARPEWVIDQ VFDLFDKLGA TNEQLDFPIV YASGLNGYAG LEDTVRDGDM TPLYEAIMQH APKPEVDPEG PFQMRISQLD YNNFVGVIGI GRIQRGTLKK NMQVAVIDRE GKKRNGKVLQ VLGFMGLERI EQDSAEAGDI VAISGIQELT ISDTICAPDF PEALPALTVD EPTISMTFQV NNSPFAGNKD LSGGKFLTSR QIKDRLDREK VHNVALKVEQ LEDADKFLVS GRGELHLSVL IENMRREGYE LAVSRPEVII KEIDGQLMEP IEQLVVDIEE IHQGGVMEKL GTRKGQLKNM ESDGKGRVRL EYQIPARGLI GFQNEFKTLT QGSGLLFHVF DHYGPKEQGA IAKRINGVMI ANAPGTTPAY SLGPLQERGK LFAAEGDNVY EGQLVGIHSK DNDLTVNAIK TKPLTNMRAS GKDDAIQLTP AIKYSLEQAL DFIEDDELVE ITPKEIRLRK KFLTESDRKK ASRGG
NT Sequence
ATGTCCATCG AAAATCTTCG CAACATCGCC ATCGTCGCCC ACGTCGACCA TGGCAAGACC ACCCTGGTCG ACCAGCTGCT GAAGCAGTCC GGCACCCTGT CCGAGCGCAC CGTCCTCGCC GAGCGCGTGA TGGACAGCAA CGACCAGGAA AAGGAACGCG GCATCACCAT CCTGGCCAAG AACACCGCCA TCACCTGGGA AGACAAGAAG ACCGGCATCA AGAACCGGAT CAACATCGTC GACACCCCCG GCCACGCCGA CTTCGGTGGT GAGGTCGAGC GCGTGCTGTC GATGGTCGAT ACCGTGCTGA TCCTGGTCGA CGCGATGGAC GGCCCGATGC CGCAGACCCG CTTCGTGACC CAGAAGGCCT TCGCGATGGG CTTCAAGCCG ATCGTCGTGG TCAACAAGGT CGACCGTCCG GGCGCCCGTC CGGAATGGGT GATCGACCAG GTCTTCGACC TGTTCGACAA GCTCGGCGCC ACCAACGAGC AGCTGGACTT CCCGATCGTC TACGCCTCGG GCCTGAACGG CTATGCCGGC CTGGAAGACA CCGTGCGCGA CGGCGACATG ACCCCGCTGT ACGAAGCGAT CATGCAGCAC GCGCCGAAGC CGGAAGTGGA TCCGGAAGGC CCGTTCCAGA TGCGCATCAG CCAGCTGGAC TACAACAACT TCGTGGGCGT GATCGGCATC GGCCGCATCC AGCGCGGCAC CCTGAAGAAG AACATGCAGG TCGCGGTCAT CGACCGTGAA GGCAAGAAGC GCAACGGCAA GGTGCTGCAG GTGCTGGGCT TCATGGGCCT GGAGCGCATC GAGCAGGATT CGGCCGAGGC CGGCGACATC GTGGCCATCT CCGGCATCCA GGAGCTGACC ATCTCCGACA CCATCTGCGC ACCGGACTTC CCGGAAGCGC TGCCGGCGCT GACCGTCGAC GAGCCGACCA TCTCGATGAC CTTCCAGGTC AACAACTCGC CGTTCGCCGG CAACAAGGAC CTGTCCGGTG GCAAGTTCCT GACCAGCCGC CAGATCAAGG ACCGCCTGGA CCGTGAAAAG GTCCACAACG TGGCCCTGAA GGTCGAGCAG CTGGAAGACG CCGACAAGTT CCTGGTCTCC GGCCGTGGCG AACTGCACCT GTCGGTGCTG ATCGAGAACA TGCGCCGCGA AGGCTACGAG CTGGCCGTGT CGCGCCCGGA AGTGATCATC AAGGAAATCG ACGGCCAGCT GATGGAGCCG ATCGAGCAGC TGGTGGTGGA CATCGAAGAA ATCCACCAGG GCGGCGTGAT GGAAAAGCTG GGCACCCGCA AGGGCCAGCT GAAGAACATG GAATCGGACG GCAAGGGCCG CGTGCGCCTG GAGTACCAGA TCCCGGCCCG TGGCCTGATC GGTTTCCAGA ACGAGTTCAA GACCCTGACC CAGGGTTCGG GCCTGCTGTT CCACGTGTTC GACCACTACG GTCCGAAGGA ACAGGGCGCG ATTGCCAAGC GCATCAACGG CGTGATGATC GCCAATGCAC CGGGCACCAC GCCGGCCTAC TCGCTGGGCC CGCTGCAGGA GCGCGGCAAG CTGTTCGCTG CTGAAGGCGA CAACGTGTAT GAAGGTCAGC TGGTCGGCAT CCACTCCAAG GACAACGACC TGACCGTCAA CGCGATCAAG ACCAAGCCGC TGACCAACAT GCGCGCTTCG GGCAAGGACG ATGCGATCCA GCTGACCCCG GCGATCAAGT ACTCGCTGGA ACAGGCCCTG GACTTCATCG AAGACGACGA GCTGGTCGAG ATCACCCCGA AGGAGATCCG CCTGCGCAAG AAGTTCCTGA CCGAGAGCGA TCGCAAGAAG GCTTCGCGCG GCGGC
Details for StmaA.01271.b.B1.GE43296
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 85
PERCENT COVERAGE: 56
Validated AA Sequence
MAHHHHHHMS IENLRNIAIV AHVDHGKTTL VDQLLKQSGT LSERTVLAER VMDSNDQEKE RGITILAKNT AITWEDKKTG IKNRINIVDT PGHADFGGEV ERVLSMVDTV LILVDAMDGP MPQTRFVTQK AFAMGFKPIV VVNKVDRPGA RPEWVIDQVF DLFDKLGATN EQLDFPIVYA SGLNGYAGLE DTVRDGDMTP LYEAIMQHAP KPEVDPEGPF QMRISQLDYN NFVGVIGIGR IQRGTLKKNM XVAVIDREGK KRNGKVLXXL GXXGLXRIEQ XSXXAXXXXX XSXIXELTXS XXXXAPXXXX AXXALTVDEP XXXMXXGQXL AVRXQQGPXR WQVPXQPPDQ GPPGP
Validated NT Sequence
tgnnatcgct ctcggtcagg aacttcttgc gcaggcggat ctccttcggg gtgatctcga ccagctcgtc gtcttcgatg aagtccaggg cctgttccag cgagtacttg atcgccgggg tcagctggat cgcatcgtcc ttgcccgaag cgcgcatgtt ggtcagcggc ttggtcttga tcgcgttgac ggtcaggtcg ttgtccttgg agtggatgcc gaccagctga ccttcataca cgttgtcgcc ttcagcagcg aacagcttgc cgcgctcctg cagcgggccc agcgagtagg ccggcgtggt gcccggtgca ttggcgatca tcacgccgtt gatgcgcttg gcaatcgcgc cctgttcctt cggaccgtag tggtcgaaca cgtggaacag caggcccgaa ccctgggtca gggtcttgaa ctcgttctgg aaaccgatca ggccacgggc cgggatctgg tactccaggc gcacgcggcc cttgccgtcc gattccatgt tcttcagctg gcccttgcgg gtgcccagct tttccatcac gccgccctgg tggatttctt cgatgtccac caccagctgc tcgatcggct ccatcagctg gccgtcgatt tccttgatga tcacttccgg gcgcgacacg gccagctcgt agccttcgcg gcgcatgttc tcgatcagca ccgacaggtg cagttcgcca cggccggana ncaggaactt gtcggcgtct tccagctgct cgaccttcnn ggncacgttg tggacctttt cacggtccng gcggtccttg atctggcggc tggncaggaa cttgccaccg gannggtcct tgttgccngc gaacngcgag tngttgacct nnnnncatcn anatggncgg ctcgtcgacg gtcagcgcnn gcnncgcttn cngnnantnn ggngcncaga nggnntcgga ganggtcagc tccnngatnc nggagannnc cncganntng cnggcntngg nnganncctg ctcgatgcgn tcnaggccca ngnngcccag cnccngcagc accttgccgt tgcgcttctt nccttcacgg tcgatgaccg cgacntgcat gttcttcttc agggtgccgc gctggatgcg gccgatgccg atcacgccca cgaagttgtt gtagtccagc tggctgatgc gcatctggaa cggnccttcc ggatccactt ccggcttcgg cgcgtgctgc atgatcgctt cgtacagcgg ggtcatgtcg ccgtcgcgca cggtgtcttc caggccggca tagccgttca ggcccgaggc gtagacgatc gggaagtcca gctgctcgtt ggtggcgccg agcttgtcga acaggtcgaa gacctggtcg atcacccatt ccggacgggc gcccggacgg tcgaccttgt tgaccacgac gatcggcttg aagcccatcg cgaaggcctt ctgggtcacg aagcgggtct gcggcatcgg gccgtccatc gcgtcgacca ggatcagcac ggtatcgacc atcgacagca cgcgctcgac ctcaccaccg aagtcggcgt ggccgggggt gtcgacgatg ttgatccggt tcttgatgcc ggtcttcttg tcttcccagg tgatggcggt gttcttggcc aggatggtga tgccgcgttc cttttcctgg tcgttgctgt ccatcacgcg ctcggcgagg acggtgcgct cggacagggt gccggactgc ttcagcagct ggtcgaccag ggtggtcttg ccatggtcga cgtgggcgac gatggcgatg ttgcgaagat tttcgatgga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS IENLRNIAIV AHVDHGKTTL VDQLLKQSGT LSERTVLAER VMDSNDQEKE RGITILAKNT AITWEDKKTG IKNRINIVDT PGHADFGGEV ERVLSMVDTV LILVDAMDGP MPQTRFVTQK AFAMGFKPIV VVNKVDRPGA RPEWVIDQVF DLFDKLGATN EQLDFPIVYA SGLNGYAGLE DTVRDGDMTP LYEAIMQHAP KPEVDPEGPF QMRISQLDYN NFVGVIGIGR IQRGTLKKNM QVAVIDREGK KRNGKVLQVL GFMGLERIEQ DSAEAGDIVA ISGIQELTIS DTICAPDFPE ALPALTVDEP TISMTFQVNN SPFAGNKDLS GGKFLTSRQI KDRLDREKVH NVALKVEQLE DADKFLVSGR GELHLSVLIE NMRREGYELA VSRPEVIIKE IDGQLMEPIE QLVVDIEEIH QGGVMEKLGT RKGQLKNMES DGKGRVRLEY QIPARGLIGF QNEFKTLTQG SGLLFHVFDH YGPKEQGAIA KRINGVMIAN APGTTPAYSL GPLQERGKLF AAEGDNVYEG QLVGIHSKDN DLTVNAIKTK PLTNMRASGK DDAIQLTPAI KYSLEQALDF IEDDELVEIT PKEIRLRKKF LTESDRKKAS RGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca tcgaaaatct tcgcaacatc gccatcgtcg cccacgtcga ccatggcaag accaccctgg tcgaccagct gctgaagcag tccggcaccc tgtccgagcg caccgtcctc gccgagcgcg tgatggacag caacgaccag gaaaaggaac gcggcatcac catcctggcc aagaacaccg ccatcacctg ggaagacaag aagaccggca tcaagaaccg gatcaacatc gtcgacaccc ccggccacgc cgacttcggt ggtgaggtcg agcgcgtgct gtcgatggtc gataccgtgc tgatcctggt cgacgcgatg gacggcccga tgccgcagac ccgcttcgtg acccagaagg ccttcgcgat gggcttcaag ccgatcgtcg tggtcaacaa ggtcgaccgt ccgggcgccc gtccggaatg ggtgatcgac caggtcttcg acctgttcga caagctcggc gccaccaacg agcagctgga cttcccgatc gtctacgcct cgggcctgaa cggctatgcc ggcctggaag acaccgtgcg cgacggcgac atgaccccgc tgtacgaagc gatcatgcag cacgcgccga agccggaagt ggatccggaa ggcccgttcc agatgcgcat cagccagctg gactacaaca acttcgtggg cgtgatcggc atcggccgca tccagcgcgg caccctgaag aagaacatgc aggtcgcggt catcgaccgt gaaggcaaga agcgcaacgg caaggtgctg caggtgctgg gcttcatggg cctggagcgc atcgagcagg attcggccga ggccggcgac atcgtggcca tctccggcat ccaggagctg accatctccg acaccatctg cgcaccggac ttcccggaag cgctgccggc gctgaccgtc gacgagccga ccatctcgat gaccttccag gtcaacaact cgccgttcgc cggcaacaag gacctgtccg gtggcaagtt cctgaccagc cgccagatca aggaccgcct ggaccgtgaa aaggtccaca acgtggccct gaaggtcgag cagctggaag acgccgacaa gttcctggtc tccggccgtg gcgaactgca cctgtcggtg ctgatcgaga acatgcgccg cgaaggctac gagctggccg tgtcgcgccc ggaagtgatc atcaaggaaa tcgacggcca gctgatggag ccgatcgagc agctggtggt ggacatcgaa gaaatccacc agggcggcgt gatggaaaag ctgggcaccc gcaagggcca gctgaagaac atggaatcgg acggcaaggg ccgcgtgcgc ctggagtacc agatcccggc ccgtggcctg atcggtttcc agaacgagtt caagaccctg acccagggtt cgggcctgct gttccacgtg ttcgaccact acggtccgaa ggaacagggc gcgattgcca agcgcatcaa cggcgtgatg atcgccaatg caccgggcac cacgccggcc tactcgctgg gcccgctgca ggagcgcggc aagctgttcg ctgctgaagg cgacaacgtg tatgaaggtc agctggtcgg catccactcc aaggacaacg acctgaccgt caacgcgatc aagaccaagc cgctgaccaa catgcgcgct tcgggcaagg acgatgcgat ccagctgacc ccggcgatca agtactcgct ggaacaggcc ctggacttca tcgaagacga cgagctggtc gagatcaccc cgaaggagat ccgcctgcgc aagaagttcc tgaccgagag cgatcgcaag aaggcttcgc gcggcggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for StmaA.01271.b.B1.PW38773
PURIFICATION DATe: 12/16/2019
CONCENTRATION: 5mg/ml
OBSERVED MW: 70kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 85
PERCENT COVERAGE: 56
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS IENLRNIAIV AHVDHGKTTL VDQLLKQSGT LSERTVLAER VMDSNDQEKE RGITILAKNT AITWEDKKTG IKNRINIVDT PGHADFGGEV ERVLSMVDTV LILVDAMDGP MPQTRFVTQK AFAMGFKPIV VVNKVDRPGA RPEWVIDQVF DLFDKLGATN EQLDFPIVYA SGLNGYAGLE DTVRDGDMTP LYEAIMQHAP KPEVDPEGPF QMRISQLDYN NFVGVIGIGR IQRGTLKKNM XVAVIDREGK KRNGKVLXXL GXXGLXRIEQ XSXXAXXXXX XSXIXELTXS XXXXAPXXXX AXXALTVDEP XXXMXXGQXL AVRXQQGPXR WQVPXQPPDQ GPPGP
Validated NT Sequence
tgnnatcgct ctcggtcagg aacttcttgc gcaggcggat ctccttcggg gtgatctcga ccagctcgtc gtcttcgatg aagtccaggg cctgttccag cgagtacttg atcgccgggg tcagctggat cgcatcgtcc ttgcccgaag cgcgcatgtt ggtcagcggc ttggtcttga tcgcgttgac ggtcaggtcg ttgtccttgg agtggatgcc gaccagctga ccttcataca cgttgtcgcc ttcagcagcg aacagcttgc cgcgctcctg cagcgggccc agcgagtagg ccggcgtggt gcccggtgca ttggcgatca tcacgccgtt gatgcgcttg gcaatcgcgc cctgttcctt cggaccgtag tggtcgaaca cgtggaacag caggcccgaa ccctgggtca gggtcttgaa ctcgttctgg aaaccgatca ggccacgggc cgggatctgg tactccaggc gcacgcggcc cttgccgtcc gattccatgt tcttcagctg gcccttgcgg gtgcccagct tttccatcac gccgccctgg tggatttctt cgatgtccac caccagctgc tcgatcggct ccatcagctg gccgtcgatt tccttgatga tcacttccgg gcgcgacacg gccagctcgt agccttcgcg gcgcatgttc tcgatcagca ccgacaggtg cagttcgcca cggccggana ncaggaactt gtcggcgtct tccagctgct cgaccttcnn ggncacgttg tggacctttt cacggtccng gcggtccttg atctggcggc tggncaggaa cttgccaccg gannggtcct tgttgccngc gaacngcgag tngttgacct nnnnncatcn anatggncgg ctcgtcgacg gtcagcgcnn gcnncgcttn cngnnantnn ggngcncaga nggnntcgga ganggtcagc tccnngatnc nggagannnc cncganntng cnggcntngg nnganncctg ctcgatgcgn tcnaggccca ngnngcccag cnccngcagc accttgccgt tgcgcttctt nccttcacgg tcgatgaccg cgacntgcat gttcttcttc agggtgccgc gctggatgcg gccgatgccg atcacgccca cgaagttgtt gtagtccagc tggctgatgc gcatctggaa cggnccttcc ggatccactt ccggcttcgg cgcgtgctgc atgatcgctt cgtacagcgg ggtcatgtcg ccgtcgcgca cggtgtcttc caggccggca tagccgttca ggcccgaggc gtagacgatc gggaagtcca gctgctcgtt ggtggcgccg agcttgtcga acaggtcgaa gacctggtcg atcacccatt ccggacgggc gcccggacgg tcgaccttgt tgaccacgac gatcggcttg aagcccatcg cgaaggcctt ctgggtcacg aagcgggtct gcggcatcgg gccgtccatc gcgtcgacca ggatcagcac ggtatcgacc atcgacagca cgcgctcgac ctcaccaccg aagtcggcgt ggccgggggt gtcgacgatg ttgatccggt tcttgatgcc ggtcttcttg tcttcccagg tgatggcggt gttcttggcc aggatggtga tgccgcgttc cttttcctgg tcgttgctgt ccatcacgcg ctcggcgagg acggtgcgct cggacagggt gccggactgc ttcagcagct ggtcgaccag ggtggtcttg ccatggtcga cgtgggcgac gatggcgatg ttgcgaagat tttcgatgga catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMS IENLRNIAIV AHVDHGKTTL VDQLLKQSGT LSERTVLAER VMDSNDQEKE RGITILAKNT AITWEDKKTG IKNRINIVDT PGHADFGGEV ERVLSMVDTV LILVDAMDGP MPQTRFVTQK AFAMGFKPIV VVNKVDRPGA RPEWVIDQVF DLFDKLGATN EQLDFPIVYA SGLNGYAGLE DTVRDGDMTP LYEAIMQHAP KPEVDPEGPF QMRISQLDYN NFVGVIGIGR IQRGTLKKNM QVAVIDREGK KRNGKVLQVL GFMGLERIEQ DSAEAGDIVA ISGIQELTIS DTICAPDFPE ALPALTVDEP TISMTFQVNN SPFAGNKDLS GGKFLTSRQI KDRLDREKVH NVALKVEQLE DADKFLVSGR GELHLSVLIE NMRREGYELA VSRPEVIIKE IDGQLMEPIE QLVVDIEEIH QGGVMEKLGT RKGQLKNMES DGKGRVRLEY QIPARGLIGF QNEFKTLTQG SGLLFHVFDH YGPKEQGAIA KRINGVMIAN APGTTPAYSL GPLQERGKLF AAEGDNVYEG QLVGIHSKDN DLTVNAIKTK PLTNMRASGK DDAIQLTPAI KYSLEQALDF IEDDELVEIT PKEIRLRKKF LTESDRKKAS RGG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCA TCGAAAATCT TCGCAACATC GCCATCGTCG CCCACGTCGA CCATGGCAAG ACCACCCTGG TCGACCAGCT GCTGAAGCAG TCCGGCACCC TGTCCGAGCG CACCGTCCTC GCCGAGCGCG TGATGGACAG CAACGACCAG GAAAAGGAAC GCGGCATCAC CATCCTGGCC AAGAACACCG CCATCACCTG GGAAGACAAG AAGACCGGCA TCAAGAACCG GATCAACATC GTCGACACCC CCGGCCACGC CGACTTCGGT GGTGAGGTCG AGCGCGTGCT GTCGATGGTC GATACCGTGC TGATCCTGGT CGACGCGATG GACGGCCCGA TGCCGCAGAC CCGCTTCGTG ACCCAGAAGG CCTTCGCGAT GGGCTTCAAG CCGATCGTCG TGGTCAACAA GGTCGACCGT CCGGGCGCCC GTCCGGAATG GGTGATCGAC CAGGTCTTCG ACCTGTTCGA CAAGCTCGGC GCCACCAACG AGCAGCTGGA CTTCCCGATC GTCTACGCCT CGGGCCTGAA CGGCTATGCC GGCCTGGAAG ACACCGTGCG CGACGGCGAC ATGACCCCGC TGTACGAAGC GATCATGCAG CACGCGCCGA AGCCGGAAGT GGATCCGGAA GGCCCGTTCC AGATGCGCAT CAGCCAGCTG GACTACAACA ACTTCGTGGG CGTGATCGGC ATCGGCCGCA TCCAGCGCGG CACCCTGAAG AAGAACATGC AGGTCGCGGT CATCGACCGT GAAGGCAAGA AGCGCAACGG CAAGGTGCTG CAGGTGCTGG GCTTCATGGG CCTGGAGCGC ATCGAGCAGG ATTCGGCCGA GGCCGGCGAC ATCGTGGCCA TCTCCGGCAT CCAGGAGCTG ACCATCTCCG ACACCATCTG CGCACCGGAC TTCCCGGAAG CGCTGCCGGC GCTGACCGTC GACGAGCCGA CCATCTCGAT GACCTTCCAG GTCAACAACT CGCCGTTCGC CGGCAACAAG GACCTGTCCG GTGGCAAGTT CCTGACCAGC CGCCAGATCA AGGACCGCCT GGACCGTGAA AAGGTCCACA ACGTGGCCCT GAAGGTCGAG CAGCTGGAAG ACGCCGACAA GTTCCTGGTC TCCGGCCGTG GCGAACTGCA CCTGTCGGTG CTGATCGAGA ACATGCGCCG CGAAGGCTAC GAGCTGGCCG TGTCGCGCCC GGAAGTGATC ATCAAGGAAA TCGACGGCCA GCTGATGGAG CCGATCGAGC AGCTGGTGGT GGACATCGAA GAAATCCACC AGGGCGGCGT GATGGAAAAG CTGGGCACCC GCAAGGGCCA GCTGAAGAAC ATGGAATCGG ACGGCAAGGG CCGCGTGCGC CTGGAGTACC AGATCCCGGC CCGTGGCCTG ATCGGTTTCC AGAACGAGTT CAAGACCCTG ACCCAGGGTT CGGGCCTGCT GTTCCACGTG TTCGACCACT ACGGTCCGAA GGAACAGGGC GCGATTGCCA AGCGCATCAA CGGCGTGATG ATCGCCAATG CACCGGGCAC CACGCCGGCC TACTCGCTGG GCCCGCTGCA GGAGCGCGGC AAGCTGTTCG CTGCTGAAGG CGACAACGTG TATGAAGGTC AGCTGGTCGG CATCCACTCC AAGGACAACG ACCTGACCGT CAACGCGATC AAGACCAAGC CGCTGACCAA CATGCGCGCT TCGGGCAAGG ACGATGCGAT CCAGCTGACC CCGGCGATCA AGTACTCGCT GGAACAGGCC CTGGACTTCA TCGAAGACGA CGAGCTGGTC GAGATCACCC CGAAGGAGAT CCGCCTGCGC AAGAAGTTCC TGACCGAGAG CGATCGCAAG AAGGCTTCGC GCGGCGGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT