SSGCID: Target Page

StmaA.01271.a

Translation elongation factor LepA (lepA)

CENTER ID:	StmaA.01271.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.01271.a.B1.GE43295	Full length(StmaA.01271.a)		1	597

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3334

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRNIRNFSII AHVDHGKSTL ADRIIQLCGG LQAREMEAQV LDSNPIERER GITIKAQSVS LPYLAKDGQT YHLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAAQGVEA QSVANCYTAV EQGLEVVPVI NKIDLPTADI ERAKAEIEAV IGIDASDAVP VSAKTGLNVQ DVLEAIVHRI PPPTPRDTEK LQALIIDSWF DNYLGVVSLV RVMQGEIKAG DKLQVMSTGR NHQVDNVGVF TPKRKVLPAL RAGEVGWVTA SIKDVHGAPV GDTLTLAGDP APKPLPGFQE MQPRVFAGLF PVDAEDYPDL REALDKLRLN DAALRFEPES SEAMGFGFRC GFLGMLHMEI VQERLEREYN LDLISTAPTV VYEVLKTDGS IINMDNPAKL PPVNQVEEIR EPIIRANVLT PEEYIGNIIK LCEEKRGSQI GINYLGSQVQ ISYELPMAEV VLDFFDKLKS VSRGYASLDY HFVRFDAGPF VRVDVLINGD KVDALSLIVH RSHADRRGRE LCEKMKDLIP RQMFDVAIQA AVGSQIIART TVKAMRKNVL AKCYGGDVSR KKKLLEKQKE GKKRMKQVGR VEIPQEAFLA VLQMDNK

NT Sequence

ATGCGGAACA TCCGCAACTT CTCCATCATC GCCCATGTCG ACCACGGCAA GTCCACGCTG GCCGACCGCA TCATCCAGCT CTGTGGTGGC CTGCAGGCCC GCGAGATGGA AGCGCAGGTG CTCGACTCCA ACCCGATCGA GCGCGAGCGC GGCATCACGA TCAAGGCGCA GTCGGTGTCG TTGCCGTACC TGGCCAAGGA CGGGCAGACC TACCATCTGA ACTTCATCGA CACCCCCGGC CACGTCGACT TCTCCTATGA AGTCAGCCGC TCGCTGGCCG CCTGCGAAGG CGCGCTGCTG GTGGTCGATG CGGCCCAGGG CGTGGAAGCG CAGTCAGTGG CCAACTGCTA CACCGCGGTG GAGCAGGGCC TGGAAGTGGT GCCGGTGATC AACAAGATCG ACCTGCCGAC TGCCGACATC GAGCGCGCCA AGGCCGAGAT CGAGGCCGTG ATCGGCATCG ACGCCTCCGA CGCCGTGCCG GTCAGTGCCA AGACCGGCCT GAACGTGCAG GACGTGCTGG AAGCGATCGT GCATCGCATC CCGCCGCCGA CACCGCGCGA CACCGAGAAG CTGCAGGCGC TGATCATCGA CTCCTGGTTC GACAACTACC TGGGCGTGGT CTCGCTGGTA CGCGTGATGC AGGGCGAGAT CAAGGCCGGT GACAAGCTGC AGGTGATGTC CACCGGCCGC AACCACCAGG TCGACAACGT CGGCGTGTTC ACGCCGAAGC GGAAAGTGCT GCCTGCGCTG CGTGCCGGTG AAGTGGGCTG GGTCACCGCC AGCATCAAGG ACGTGCACGG TGCGCCGGTC GGCGACACCC TGACCCTGGC CGGCGACCCG GCACCGAAGC CGCTGCCGGG CTTCCAGGAA ATGCAGCCGC GCGTGTTCGC CGGTCTGTTC CCGGTCGATG CCGAAGATTA CCCGGACCTG CGCGAAGCGC TGGACAAGCT GCGCCTGAAC GATGCCGCGC TGCGTTTCGA GCCGGAAAGC TCCGAGGCGA TGGGCTTCGG CTTCCGCTGC GGCTTCCTGG GCATGCTGCA CATGGAAATC GTGCAGGAGC GACTGGAGCG CGAGTACAAC CTGGACCTGA TCAGCACCGC GCCGACGGTG GTGTATGAAG TCCTGAAGAC CGATGGCTCG ATCATCAACA TGGACAACCC GGCCAAGCTG CCGCCGGTGA ACCAGGTCGA GGAGATCCGC GAGCCGATCA TCCGTGCCAA CGTGCTCACT CCCGAGGAGT ACATCGGCAA CATCATCAAG CTGTGCGAAG AAAAGCGCGG CAGCCAGATC GGCATCAACT ACCTGGGCAG CCAGGTGCAG ATCAGCTATG AGCTGCCGAT GGCCGAGGTA GTGCTGGACT TCTTCGACAA GCTGAAGTCG GTCAGCCGTG GCTACGCCTC GCTGGACTAC CACTTCGTCC GCTTCGACGC CGGCCCGTTC GTGCGCGTGG ACGTGCTGAT CAACGGCGAC AAGGTCGACG CACTGTCGCT GATCGTGCAC CGCAGCCATG CCGACCGTCG TGGCCGTGAG CTGTGCGAGA AGATGAAGGA CCTGATTCCG CGCCAGATGT TCGACGTCGC CATCCAGGCT GCGGTCGGCT CGCAGATCAT CGCCCGTACC ACGGTCAAGG CCATGCGCAA GAACGTGCTG GCCAAGTGCT ATGGTGGCGA CGTTTCGCGC AAGAAGAAGC TTCTGGAAAA GCAGAAGGAA GGCAAGAAGC GCATGAAGCA GGTCGGCCGC GTGGAGATCC CGCAGGAAGC GTTCCTGGCC GTGCTGCAGA TGGACAACAA G

Details for StmaA.01271.a.B1.GE43295

HARVESTED ON:	6/12/2019
SEQUENCED ON:	6/18/2019
EXPECTED MW:	67kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	80
PERCENT COVERAGE:	62

Validated AA Sequence

Validated NT Sequence

aggaacgctt cctgcgggat ctccacgcgg ccgacctgct tcatgcgctt cttgccttcc ttctgctttt ccagaagctt cttcttgcgc gaaacgtcgc caccatagca cttggccagc acgttcttgc gcatggcctt gaccgtggta cgggcgatga tctgcgagcc gaccgcagcc tggatggcga cgtcgaacat ctggcgcgga atcaggtcct tcatcttctc gcacagctca cggccacgac ggtcggcatg gctgcggtgc acgatcagcg acagtgcgtc gaccttgtcg ccgttgatca gcacgtccac gcgcacgaac gggccggcgt cgaagcggac gaagtggtag tccagcgagg cgtagccacg gctgaccgac ttcagcttgt cgaagaagtc cagcactacc tcggccatcg gcagctcata gctgatctgc acctggctgc ccaggtagtt gatgccgatc tggctgccgc gcttttcttc gcacagcttg atgatgttgc cgatgtactc ctcgggagtg agcacgttgg cacggatgat cggctcgcgg atctcctcga cctggttcac cggcggcagc ttggccgggt tgtccatgtt gatgatcgag ccatcggtct tcangacttc atacaccacc gtcggcgcgg tgctgatcan gtccnggttg tactcgcgct ccagtcgctc ctgcacgant tccatgtgca gcatgcccag gaagccgcag cggaagccga agcccatcgc ctcgganctt tccggntcna aacgcagcgc ggcatcgttc nnngcanctt gtccagcgct tcncgcnnnc ngggtaatct tcggcatcga ccgggaacag ancngngaac acgcgcggct gcatttnntg naanccnggc ancnnnttcg gtncnggntc nccnnccnng ntcanggtgt ngccgaccgg cncnccgtgc acgtcnntga tgcngncggt gncccanccc acttcncngg cacgcagcgc aggcagcact ttccgnttng gcgtgaacac gcngangttg tcgacctggt ggttgcggcc ggtggacatc acctgcagct tntcaccgnc cttgatctcg ccntgcatca cgcgtaccag cgagaccacg cccaggtagt tgtcgaacca ggagtcgatg atcagcgcct gcagcttctc ggtgtcgcgc ggtgtcggcg gcgggatgcg atgcacgatc gcttccagca cgtcctgcac gttcaggccg gtcttggcac tgaccggcac ggcgtcggag gcgtcgatgc cgatcacggc ctcgatctcg gccttggcgc gctcgatgtc ggcagtcggc aggtcgatct tgttgatcac cggcaccact tccaggccct gctccaccgc ggtgtagcag ttggccactg actgcgcttc cacgccctgg gccgcatcga ccaccagcag cgcgccttcg caggcggcca gcgagcggct gacttcatag gagaagtcga cgtggccggg ggtgtcgatg aagttcagat ggtaggtctg cccgtccttg gccaggtacg gcaacgacac cgactgcgcc ttgatcgtga tgccgcgctc gcgctcgatc gggttggagt cgagcacctg cgcttccatc tcgcgggcct gcaggccacc acagagctgg atgatgcggt cggccagcgt ggacttgccg tggtcgacat gggcgatgat ggagaagttg cggatgttcc gcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMR NIRNFSIIAH VDHGKSTLAD RIIQLCGGLQ AREMEAQVLD SNPIERERGI TIKAQSVSLP YLAKDGQTYH LNFIDTPGHV DFSYEVSRSL AACEGALLVV DAAQGVEAQS VANCYTAVEQ GLEVVPVINK IDLPTADIER AKAEIEAVIG IDASDAVPVS AKTGLNVQDV LEAIVHRIPP PTPRDTEKLQ ALIIDSWFDN YLGVVSLVRV MQGEIKAGDK LQVMSTGRNH QVDNVGVFTP KRKVLPALRA GEVGWVTASI KDVHGAPVGD TLTLAGDPAP KPLPGFQEMQ PRVFAGLFPV DAEDYPDLRE ALDKLRLNDA ALRFEPESSE AMGFGFRCGF LGMLHMEIVQ ERLEREYNLD LISTAPTVVY EVLKTDGSII NMDNPAKLPP VNQVEEIREP IIRANVLTPE EYIGNIIKLC EEKRGSQIGI NYLGSQVQIS YELPMAEVVL DFFDKLKSVS RGYASLDYHF VRFDAGPFVR VDVLINGDKV DALSLIVHRS HADRRGRELC EKMKDLIPRQ MFDVAIQAAV GSQIIARTTV KAMRKNVLAK CYGGDVSRKK KLLEKQKEGK KRMKQVGRVE IPQEAFLAVL QMDNK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgga acatccgcaa cttctccatc atcgcccatg tcgaccacgg caagtccacg ctggccgacc gcatcatcca gctctgtggt ggcctgcagg cccgcgagat ggaagcgcag gtgctcgact ccaacccgat cgagcgcgag cgcggcatca cgatcaaggc gcagtcggtg tcgttgccgt acctggccaa ggacgggcag acctaccatc tgaacttcat cgacaccccc ggccacgtcg acttctccta tgaagtcagc cgctcgctgg ccgcctgcga aggcgcgctg ctggtggtcg atgcggccca gggcgtggaa gcgcagtcag tggccaactg ctacaccgcg gtggagcagg gcctggaagt ggtgccggtg atcaacaaga tcgacctgcc gactgccgac atcgagcgcg ccaaggccga gatcgaggcc gtgatcggca tcgacgcctc cgacgccgtg ccggtcagtg ccaagaccgg cctgaacgtg caggacgtgc tggaagcgat cgtgcatcgc atcccgccgc cgacaccgcg cgacaccgag aagctgcagg cgctgatcat cgactcctgg ttcgacaact acctgggcgt ggtctcgctg gtacgcgtga tgcagggcga gatcaaggcc ggtgacaagc tgcaggtgat gtccaccggc cgcaaccacc aggtcgacaa cgtcggcgtg ttcacgccga agcggaaagt gctgcctgcg ctgcgtgccg gtgaagtggg ctgggtcacc gccagcatca aggacgtgca cggtgcgccg gtcggcgaca ccctgaccct ggccggcgac ccggcaccga agccgctgcc gggcttccag gaaatgcagc cgcgcgtgtt cgccggtctg ttcccggtcg atgccgaaga ttacccggac ctgcgcgaag cgctggacaa gctgcgcctg aacgatgccg cgctgcgttt cgagccggaa agctccgagg cgatgggctt cggcttccgc tgcggcttcc tgggcatgct gcacatggaa atcgtgcagg agcgactgga gcgcgagtac aacctggacc tgatcagcac cgcgccgacg gtggtgtatg aagtcctgaa gaccgatggc tcgatcatca acatggacaa cccggccaag ctgccgccgg tgaaccaggt cgaggagatc cgcgagccga tcatccgtgc caacgtgctc actcccgagg agtacatcgg caacatcatc aagctgtgcg aagaaaagcg cggcagccag atcggcatca actacctggg cagccaggtg cagatcagct atgagctgcc gatggccgag gtagtgctgg acttcttcga caagctgaag tcggtcagcc gtggctacgc ctcgctggac taccacttcg tccgcttcga cgccggcccg ttcgtgcgcg tggacgtgct gatcaacggc gacaaggtcg acgcactgtc gctgatcgtg caccgcagcc atgccgaccg tcgtggccgt gagctgtgcg agaagatgaa ggacctgatt ccgcgccaga tgttcgacgt cgccatccag gctgcggtcg gctcgcagat catcgcccgt accacggtca aggccatgcg caagaacgtg ctggccaagt gctatggtgg cgacgtttcg cgcaagaaga agcttctgga aaagcagaag gaaggcaaga agcgcatgaa gcaggtcggc cgcgtggaga tcccgcagga agcgttcctg gccgtgctgc agatggacaa caagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat