StmaA.01254.a

Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5) (dfp)

CENTER ID: StmaA.01254.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01254.a.B1.GE43294 Full length(StmaA.01254.a) 1 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.376

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADSPNASPA PARALERQKL LLCVGGGIAA YKALELVRRL RDAGAQVQVA MTAGAQQFVT PLSFQALSGQ PTRTTLWDSA AEQAMGHIEL ARWADRIVVA PGTADLLARL AQGHADDLVS TLCLASTAPL TICPAMNHRM WLHPATQANI ALLRQRGAQV IGPVDGPLAE GESGPGRLAE PGDIVAALAA NGSAAAAVVA PETRALQGLR LLISAGPTYE DIDPVRYVGN RSSGKMGFAL AAAAATMGAQ VVLVSGPVQL PTPAGVQRVD VRSAAQMRDA VLKSLPADIY IGAAAVSDYT PRQVAPQKLK KTADSQSLVI ELVRTPDILA EVAAQTQSLK LVVGFAAETH DVEKYARGKL VDKRLDLVIA NQVGISGGGF ESDNNAATAF WQDGEQVFPA TSKRELAEQL LALIARRLQA
NT Sequence
GTGGCTGACT CCCCCAACGC TTCCCCCGCG CCGGCCCGCG CGCTGGAACG TCAGAAACTG CTGTTGTGCG TCGGAGGCGG GATCGCGGCC TACAAGGCCC TGGAACTGGT GCGGCGCCTG CGCGACGCCG GTGCCCAGGT GCAGGTGGCG ATGACCGCCG GAGCACAGCA GTTCGTCACT CCGCTCAGTT TCCAGGCCCT GTCCGGGCAG CCCACCCGCA CCACGCTGTG GGACAGCGCC GCCGAACAGG CCATGGGCCA CATCGAGCTG GCCCGCTGGG CCGACCGCAT CGTGGTCGCC CCGGGTACCG CCGATCTGCT GGCCCGGCTG GCCCAGGGCC ACGCCGATGA TCTGGTCAGC ACCCTGTGCC TGGCCAGTAC CGCGCCGCTG ACGATCTGCC CGGCGATGAA CCACCGCATG TGGCTGCATC CGGCCACCCA GGCCAACATC GCCCTGCTGC GCCAGCGCGG CGCTCAGGTG ATCGGCCCGG TCGACGGTCC GCTGGCCGAA GGCGAATCCG GTCCCGGCCG TCTGGCCGAA CCGGGCGACA TCGTTGCCGC GCTGGCTGCC AATGGCAGTG CTGCAGCGGC GGTTGTCGCA CCGGAAACCC GCGCGCTGCA GGGCCTTCGG CTGCTGATCA GCGCTGGCCC GACCTATGAA GACATCGATC CGGTGCGCTA CGTCGGCAAC CGCAGCAGCG GCAAGATGGG CTTTGCCCTG GCCGCGGCCG CCGCGACGAT GGGCGCCCAG GTGGTGCTGG TCAGCGGCCC GGTACAGCTG CCGACGCCAG CCGGCGTGCA GCGCGTGGAC GTGCGCTCGG CCGCGCAGAT GCGCGATGCC GTGCTGAAGT CGTTGCCGGC CGACATCTAT ATCGGTGCCG CCGCGGTGTC CGACTACACG CCACGCCAGG TCGCGCCGCA GAAGCTGAAG AAGACCGCCG ACAGCCAGTC GCTGGTGATC GAGCTGGTGC GCACGCCGGA CATCCTCGCC GAAGTCGCTG CACAGACGCA GTCGCTGAAG CTGGTGGTCG GCTTCGCCGC CGAGACCCAC GACGTGGAGA AATATGCGCG TGGCAAGCTG GTCGACAAGC GCCTGGACCT GGTGATCGCC AACCAGGTCG GCATCAGTGG CGGCGGTTTC GAGAGCGACA ACAATGCCGC CACGGCCTTC TGGCAGGACG GTGAACAGGT ATTCCCGGCC ACCTCCAAGC GCGAGCTGGC CGAACAACTG CTGGCGCTGA TCGCGCGGAG ACTCCAGGCA
Details for StmaA.01254.a.B1.GE43294
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV ADSPNASPAP ARALERQKLL LCVGGGIAAY KALELVRRLR DAGAQVQVAM TAGAQQFVTP LSFQALSGQP TRTTLWDSAA EQAMGHIELA RWADRIVVAP GTADLLARLA QGHADDLVST LCLASTAPLT ICPAMNHRMW LHPATQANIA LLRQRGAQVI GPVDGPLAEG ESGPGRLAEP GDIVAALAAN GSAAAAVVAP ETRALQGLRL LISAGPTYED IDPVRYVGNR SSGKMGFALA AAAATMGAQV VLVSGPVQLP TPAGVQRVDV RSAAQMRDAV LKSLPADIYI GAAAVSDYTP RQVAPQKLKK TADSQSLVIE LVRTPDILAE VAAQTQSLKL VVGFAAETHD VEKYARGKLV DKRLDLVIAN QVGISGGGFE SDNNAATAFW QDGEQVFPAT SKRELAEQLL
Validated NT Sequence
gccagcagtt gttcggccag ctcgcgcttg gaggtggccg ggaatacctg ttcaccgtcc tgccagaagg ccgtggcggc attgttgtcg ctctcgaaac cgccgccact gatgccgacc tggttggcga tcaccaggtc caggcgcttg tcgaccagct tgccacgcgc atatttctcc acgtcgtggg tctcggcggc gaagccgacc accagcttca gcgactgcgt ctgtgcagcg acttcggcga ggatgtccgg cgtgcgcacc agctcgatca ccagcgactg gctgtcggcg gtcttcttca gcttctgcgg cgcgacctgg cgtggcgtgt agtcggacac cgcggcggca ccgatataga tgtcggccgg caacgacttc agcacggcat cgcgcatctg cgcggccgag cgcacgtcca cgcgctgcac gccggctggc gtcggcagct gtaccgggcc gctgaccagc accacctggg cgcccatcgt cgcggcggcc gcggccaggg caaagcccat cttgccgctg ctgcggttgc cgacgtagcg caccggatcg atgtcttcat aggtcgggcc agcgctgatc agcagccgaa ggccctgcag cgcgcgggtt tccggtgcga caaccgccgc tgcagcactg ccattggcag ccagcgcggc aacgatgtcg cccggttcgg ccagacggcc gggaccggat tcgccttcgg ccagcggacc gtcgaccggg ccgatcacct gagcgccgcg ctggcgcagc agggcgatgt tggcctgggt ggccggatgc agccacatgc ggtggttcat cgccgggcag atcgtcagcg gcgcggtact ggccaggcac agggtgctga ccagatcatc ggcgtggccc tgggccagcc gggccagcag atcggcggta cccggggcga ccacgatgcg gtcggcccag cgggccagct cgatgtggcc catggcctgt tcggcggcgc tgtcccacag cgtggtgcgg gtgggctgcc cggacagggc ctggaaactg agcggagtga cgaactgctg tgctccggcg gtcatcgcca cctgcacctg ggcaccggcg tcgcgcaggc gccgcaccag ttccagggcc ttgtaggccg cgatcccgcc tccgacgcac aacagcagtt tctgacgttc cagcgcgcgg gccggcgcgg gggaagcgtt gggggagtca gccaccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMA DSPNASPAPA RALERQKLLL CVGGGIAAYK ALELVRRLRD AGAQVQVAMT AGAQQFVTPL SFQALSGQPT RTTLWDSAAE QAMGHIELAR WADRIVVAPG TADLLARLAQ GHADDLVSTL CLASTAPLTI CPAMNHRMWL HPATQANIAL LRQRGAQVIG PVDGPLAEGE SGPGRLAEPG DIVAALAANG SAAAAVVAPE TRALQGLRLL ISAGPTYEDI DPVRYVGNRS SGKMGFALAA AAATMGAQVV LVSGPVQLPT PAGVQRVDVR SAAQMRDAVL KSLPADIYIG AAAVSDYTPR QVAPQKLKKT ADSQSLVIEL VRTPDILAEV AAQTQSLKLV VGFAAETHDV EKYARGKLVD KRLDLVIANQ VGISGGGFES DNNAATAFWQ DGEQVFPATS KRELAEQLLA LIARRLQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggctg actcccccaa cgcttccccc gcgccggccc gcgcgctgga acgtcagaaa ctgctgttgt gcgtcggagg cgggatcgcg gcctacaagg ccctggaact ggtgcggcgc ctgcgcgacg ccggtgccca ggtgcaggtg gcgatgaccg ccggagcaca gcagttcgtc actccgctca gtttccaggc cctgtccggg cagcccaccc gcaccacgct gtgggacagc gccgccgaac aggccatggg ccacatcgag ctggcccgct gggccgaccg catcgtggtc gccccgggta ccgccgatct gctggcccgg ctggcccagg gccacgccga tgatctggtc agcaccctgt gcctggccag taccgcgccg ctgacgatct gcccggcgat gaaccaccgc atgtggctgc atccggccac ccaggccaac atcgccctgc tgcgccagcg cggcgctcag gtgatcggcc cggtcgacgg tccgctggcc gaaggcgaat ccggtcccgg ccgtctggcc gaaccgggcg acatcgttgc cgcgctggct gccaatggca gtgctgcagc ggcggttgtc gcaccggaaa cccgcgcgct gcagggcctt cggctgctga tcagcgctgg cccgacctat gaagacatcg atccggtgcg ctacgtcggc aaccgcagca gcggcaagat gggctttgcc ctggccgcgg ccgccgcgac gatgggcgcc caggtggtgc tggtcagcgg cccggtacag ctgccgacgc cagccggcgt gcagcgcgtg gacgtgcgct cggccgcgca gatgcgcgat gccgtgctga agtcgttgcc ggccgacatc tatatcggtg ccgccgcggt gtccgactac acgccacgcc aggtcgcgcc gcagaagctg aagaagaccg ccgacagcca gtcgctggtg atcgagctgg tgcgcacgcc ggacatcctc gccgaagtcg ctgcacagac gcagtcgctg aagctggtgg tcggcttcgc cgccgagacc cacgacgtgg agaaatatgc gcgtggcaag ctggtcgaca agcgcctgga cctggtgatc gccaaccagg tcggcatcag tggcggcggt ttcgagagcg acaacaatgc cgccacggcc ttctggcagg acggtgaaca ggtattcccg gccacctcca agcgcgagct ggccgaacaa ctgctggcgc tgatcgcgcg gagactccag gcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat