StmaA.01187.b
Glutamyl-tRNA synthetase (EC 6.1.1.17) (gltX)
| CENTER ID: | StmaA.01187.b |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01187.b.B1.GE43292 | Full length(StmaA.01187.b) | 1 | 467 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01187.b.B1.PW38726 | Full length(StmaA.01187.b) | 1 | 467 |
Structures
7K86
DEPOSITED: 9/25/2020
DETERMINATION: XRay
CLONE: StmaA.01187.b.B1.GE43292
PROTEIN: StmaA.01187.b.B1.PW38726
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.1379 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTCRTRFAPS PTGYLHIGGA RTALYCWLEA RHRGGEFVLR IEDTDRERST QGAIDAILEA MEWLGLDYDE GPIYQTDRVA RYLEVAEQLV ADGKAYYAYE TREELDAMRE AAMARQEKPR YNGAARDLGL PRRDDPNRVI RFKNPLEGTV VFDDLIKGRI EIANSELDDM VIFRPDGYPT YNFAVVVDDW DMGITEVIRG DDHINNTPRQ INLYEGIGAP VPKFGHMPMI LDEQGAKLSK RTGAADVMQY KDAGYLPDAL LSYLARLGWS HGDQELFSRQ ELIELFDVKD CNSKASRLDM AKLGWVNQHF LKTEDVAAIV PHLVYQLQKL GLDVAAGPAP EDVVVALRER VQTLKEMAEK AVVWYQPLTE YDEAAVAKHF KAGAEVALGK ARELLAALPE WTAESVGVAL HDAAAALEIG MGKVAQPLRV AITGTQVSPD ISHTVYLAGR EQALKRIDVA ITKVATA
NT Sequence
ATGACCTGCC GCACCCGCTT CGCCCCCAGT CCCACCGGCT ACCTGCACAT CGGTGGTGCC CGCACTGCGC TGTACTGCTG GCTGGAGGCC CGCCACCGTG GCGGCGAGTT CGTGCTGCGT ATCGAGGACA CCGACCGTGA GCGCAGCACC CAGGGCGCGA TCGACGCCAT CCTGGAGGCG ATGGAGTGGC TGGGCCTGGA CTATGACGAA GGCCCGATCT ACCAGACCGA CCGCGTCGCC CGTTACCTGG AAGTGGCCGA ACAGCTGGTG GCCGACGGCA AGGCGTATTA CGCATACGAG ACCCGCGAAG AGCTGGACGC GATGCGCGAG GCCGCCATGG CCAGGCAGGA AAAGCCGCGC TACAACGGAG CCGCGCGTGA CCTGGGCCTG CCGCGCAGGG ATGATCCGAA CCGCGTCATC CGCTTCAAGA ACCCGCTGGA AGGTACGGTG GTGTTCGACG ACCTGATCAA GGGCCGCATC GAGATCGCCA ACAGCGAGCT GGATGACATG GTCATCTTCC GCCCGGACGG CTACCCCACC TACAACTTCG CGGTGGTGGT GGACGACTGG GACATGGGCA TCACCGAGGT CATCCGCGGC GACGACCACA TCAACAACAC CCCGCGCCAG ATCAACCTGT ACGAAGGCAT CGGTGCGCCT GTGCCGAAGT TCGGCCACAT GCCGATGATC CTGGACGAGC AGGGCGCCAA GCTGTCCAAG CGCACCGGCG CGGCCGACGT GATGCAGTAC AAGGACGCCG GCTACCTGCC CGATGCGCTG CTGAGCTACC TGGCCCGCCT GGGCTGGTCG CACGGTGACC AGGAGCTGTT CAGCCGCCAG GAGCTGATCG AGCTGTTCGA CGTGAAGGAC TGCAATTCCA AGGCCTCGCG CCTGGACATG GCCAAGCTGG GCTGGGTCAA CCAGCACTTC CTGAAGACCG AGGACGTGGC CGCGATCGTG CCGCACCTGG TCTACCAGCT GCAGAAGCTG GGCCTGGACG TGGCCGCTGG CCCGGCCCCG GAGGACGTGG TGGTCGCCCT GCGTGAACGC GTGCAGACCC TGAAGGAAAT GGCCGAGAAG GCCGTGGTCT GGTACCAGCC GCTGACCGAG TACGACGAAG CGGCGGTAGC CAAGCACTTC AAGGCCGGTG CCGAGGTGGC GCTGGGCAAG GCCCGCGAGC TGCTGGCCGC CCTGCCGGAA TGGACCGCCG AGTCGGTGGG CGTCGCCCTG CACGACGCGG CCGCAGCGCT GGAAATCGGC ATGGGCAAGG TCGCCCAGCC GCTGCGCGTG GCCATCACCG GCACCCAGGT CAGTCCTGAC ATTTCCCATA CCGTGTACCT GGCCGGCCGC GAGCAGGCCT TGAAACGCAT TGATGTGGCC ATCACCAAGG TAGCAACGGC C
Details for StmaA.01187.b.B1.GE43292
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMT CRTRFAPSPT GYLHIGGART ALYCWLEARH RGGEFVLRIE DTDRERSTQG AIDAILEAME WLGLDYDEGP IYQTDRVARY LEVAEQLVAD GKAYYAYETR EELDAMREAA MARQEKPRYN GAARDLGLPR RDDPNRVIRF KNPLEGTVVF DDLIKGRIEI ANSELDDMVI FRPDGYPTYN FAVVVDDWDM GITEVIRGDD HINNTPRQIN LYEGIGAPVP KFGHMPMILD EQGAKLSKRT GAXDVMQYKD AGYLPDALLS YLARLGWSHG DQELFSRQEL IELFDVKDCN SKASRLDMAK LGWVNQHFLK TEDVAAIVPH LVYQLQKLGL DVAAGPAPED VVVALRERVQ TLKEMAEKAV VWYQPLTEYD EAAVAKHFKA GAEVALGKAR ELLAALPEWT AESVGVALHD AAAALEIGMG KVAQPLRVAI TGTQVSPDIS HTVYLAGREQ ALKRID
Validated NT Sequence
acatcaatgc gtttcaaggc ctgctcgcgg ccggccaggt acacggtatg ggaaatgtca ggactgacct gggtgccggt gatggccacg cgcagcggct gggcgacctt gcccatgccg atttccagcg ctgcggccgc gtcgtgcagg gcgacgccca ccgactcggc ggtccattcc ggcagggcgg ccagcagctc gcgggccttg cccagcgcca cctcggcacc ggccttgaag tgcttggcta ccgccgcttc gtcgtactcg gtcagcggct ggtaccagac cacggccttc tcggccattt ccttcagggt ctgcacgcgt tcacgcaggg cgaccaccac gtcctccggg gccgggccag cggccacgtc caggcccagc ttctgcagct ggtagaccag gtgcggcacg atcgcggcca cgtcctcggt cttcaggaag tgctggttga cccagcccag cttggccatg tccaggcgcg aggccttgga attgcagtcc ttcacgtcga acagctcgat cagctcctgg cggctgaaca gctcctggtc accgtgcgac cagcccaggc gggccaggta gctcagcagc gcatcgggca ggtagccggc gtccttgtac tgcatcacgt cggncgcgcc ggtgcgcttg gacagcttgg cgccctgctc gtccaggatc atcggcatgt ggccgaactt cggcacaggc gcaccgatgc cttcgtacag gttgatctgg cgcggggtgt tgttgatgtg gtcgtcgccg cggatgacct cggtgatgcc catgtcccag tcgtccacca ccaccgcgaa gttgtaggtg gggtagccgt ccgggcggaa gatgaccatg tcatccagct cgctgttggc gatctcgatg cggcccttga tcaggtcgtc gaacaccacc gtaccttcca gcgggttctt gaagcggatg acgcggttcg gatcatccct gcgcggcagg cccaggtcac gcgcggctcc gttgtagcgc ggcttttcct gcctggccat ggcggcctcg cgcatcgcgt ccagctcttc gcgggtctcg tatgcgtaat acgccttgcc gtcggccacc agctgttcgg ccacttccag gtaacgggcg acgcggtcgg tctggtagat cgggccttcg tcatagtcca ggcccagcca ctccatcgcc tccaggatgg cgtcgatcgc gccctgggtg ctgcgctcac ggtcggtgtc ctcgatacgc agcacgaact cgccgccacg gtggcgggcc tccagccagc agtacagcgc agtgcgggca ccaccgatgt gcaggtagcc ggtgggactg ggggcgaagc gggtgcggca ggtcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT CRTRFAPSPT GYLHIGGART ALYCWLEARH RGGEFVLRIE DTDRERSTQG AIDAILEAME WLGLDYDEGP IYQTDRVARY LEVAEQLVAD GKAYYAYETR EELDAMREAA MARQEKPRYN GAARDLGLPR RDDPNRVIRF KNPLEGTVVF DDLIKGRIEI ANSELDDMVI FRPDGYPTYN FAVVVDDWDM GITEVIRGDD HINNTPRQIN LYEGIGAPVP KFGHMPMILD EQGAKLSKRT GAADVMQYKD AGYLPDALLS YLARLGWSHG DQELFSRQEL IELFDVKDCN SKASRLDMAK LGWVNQHFLK TEDVAAIVPH LVYQLQKLGL DVAAGPAPED VVVALRERVQ TLKEMAEKAV VWYQPLTEYD EAAVAKHFKA GAEVALGKAR ELLAALPEWT AESVGVALHD AAAALEIGMG KVAQPLRVAI TGTQVSPDIS HTVYLAGREQ ALKRIDVAIT KVATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacct gccgcacccg cttcgccccc agtcccaccg gctacctgca catcggtggt gcccgcactg cgctgtactg ctggctggag gcccgccacc gtggcggcga gttcgtgctg cgtatcgagg acaccgaccg tgagcgcagc acccagggcg cgatcgacgc catcctggag gcgatggagt ggctgggcct ggactatgac gaaggcccga tctaccagac cgaccgcgtc gcccgttacc tggaagtggc cgaacagctg gtggccgacg gcaaggcgta ttacgcatac gagacccgcg aagagctgga cgcgatgcgc gaggccgcca tggccaggca ggaaaagccg cgctacaacg gagccgcgcg tgacctgggc ctgccgcgca gggatgatcc gaaccgcgtc atccgcttca agaacccgct ggaaggtacg gtggtgttcg acgacctgat caagggccgc atcgagatcg ccaacagcga gctggatgac atggtcatct tccgcccgga cggctacccc acctacaact tcgcggtggt ggtggacgac tgggacatgg gcatcaccga ggtcatccgc ggcgacgacc acatcaacaa caccccgcgc cagatcaacc tgtacgaagg catcggtgcg cctgtgccga agttcggcca catgccgatg atcctggacg agcagggcgc caagctgtcc aagcgcaccg gcgcggccga cgtgatgcag tacaaggacg ccggctacct gcccgatgcg ctgctgagct acctggcccg cctgggctgg tcgcacggtg accaggagct gttcagccgc caggagctga tcgagctgtt cgacgtgaag gactgcaatt ccaaggcctc gcgcctggac atggccaagc tgggctgggt caaccagcac ttcctgaaga ccgaggacgt ggccgcgatc gtgccgcacc tggtctacca gctgcagaag ctgggcctgg acgtggccgc tggcccggcc ccggaggacg tggtggtcgc cctgcgtgaa cgcgtgcaga ccctgaagga aatggccgag aaggccgtgg tctggtacca gccgctgacc gagtacgacg aagcggcggt agccaagcac ttcaaggccg gtgccgaggt ggcgctgggc aaggcccgcg agctgctggc cgccctgccg gaatggaccg ccgagtcggt gggcgtcgcc ctgcacgacg cggccgcagc gctggaaatc ggcatgggca aggtcgccca gccgctgcgc gtggccatca ccggcaccca ggtcagtcct gacatttccc ataccgtgta cctggccggc cgcgagcagg ccttgaaacg cattgatgtg gccatcacca aggtagcaac ggcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for StmaA.01187.b.B1.PW38726
| PURIFICATION DATe: | 10/18/2019 |
| CONCENTRATION: | 24mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT CRTRFAPSPT GYLHIGGART ALYCWLEARH RGGEFVLRIE DTDRERSTQG AIDAILEAME WLGLDYDEGP IYQTDRVARY LEVAEQLVAD GKAYYAYETR EELDAMREAA MARQEKPRYN GAARDLGLPR RDDPNRVIRF KNPLEGTVVF DDLIKGRIEI ANSELDDMVI FRPDGYPTYN FAVVVDDWDM GITEVIRGDD HINNTPRQIN LYEGIGAPVP KFGHMPMILD EQGAKLSKRT GAXDVMQYKD AGYLPDALLS YLARLGWSHG DQELFSRQEL IELFDVKDCN SKASRLDMAK LGWVNQHFLK TEDVAAIVPH LVYQLQKLGL DVAAGPAPED VVVALRERVQ TLKEMAEKAV VWYQPLTEYD EAAVAKHFKA GAEVALGKAR ELLAALPEWT AESVGVALHD AAAALEIGMG KVAQPLRVAI TGTQVSPDIS HTVYLAGREQ ALKRID
Validated NT Sequence
acatcaatgc gtttcaaggc ctgctcgcgg ccggccaggt acacggtatg ggaaatgtca ggactgacct gggtgccggt gatggccacg cgcagcggct gggcgacctt gcccatgccg atttccagcg ctgcggccgc gtcgtgcagg gcgacgccca ccgactcggc ggtccattcc ggcagggcgg ccagcagctc gcgggccttg cccagcgcca cctcggcacc ggccttgaag tgcttggcta ccgccgcttc gtcgtactcg gtcagcggct ggtaccagac cacggccttc tcggccattt ccttcagggt ctgcacgcgt tcacgcaggg cgaccaccac gtcctccggg gccgggccag cggccacgtc caggcccagc ttctgcagct ggtagaccag gtgcggcacg atcgcggcca cgtcctcggt cttcaggaag tgctggttga cccagcccag cttggccatg tccaggcgcg aggccttgga attgcagtcc ttcacgtcga acagctcgat cagctcctgg cggctgaaca gctcctggtc accgtgcgac cagcccaggc gggccaggta gctcagcagc gcatcgggca ggtagccggc gtccttgtac tgcatcacgt cggncgcgcc ggtgcgcttg gacagcttgg cgccctgctc gtccaggatc atcggcatgt ggccgaactt cggcacaggc gcaccgatgc cttcgtacag gttgatctgg cgcggggtgt tgttgatgtg gtcgtcgccg cggatgacct cggtgatgcc catgtcccag tcgtccacca ccaccgcgaa gttgtaggtg gggtagccgt ccgggcggaa gatgaccatg tcatccagct cgctgttggc gatctcgatg cggcccttga tcaggtcgtc gaacaccacc gtaccttcca gcgggttctt gaagcggatg acgcggttcg gatcatccct gcgcggcagg cccaggtcac gcgcggctcc gttgtagcgc ggcttttcct gcctggccat ggcggcctcg cgcatcgcgt ccagctcttc gcgggtctcg tatgcgtaat acgccttgcc gtcggccacc agctgttcgg ccacttccag gtaacgggcg acgcggtcgg tctggtagat cgggccttcg tcatagtcca ggcccagcca ctccatcgcc tccaggatgg cgtcgatcgc gccctgggtg ctgcgctcac ggtcggtgtc ctcgatacgc agcacgaact cgccgccacg gtggcgggcc tccagccagc agtacagcgc agtgcgggca ccaccgatgt gcaggtagcc ggtgggactg ggggcgaagc gggtgcggca ggtcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMT CRTRFAPSPT GYLHIGGART ALYCWLEARH RGGEFVLRIE DTDRERSTQG AIDAILEAME WLGLDYDEGP IYQTDRVARY LEVAEQLVAD GKAYYAYETR EELDAMREAA MARQEKPRYN GAARDLGLPR RDDPNRVIRF KNPLEGTVVF DDLIKGRIEI ANSELDDMVI FRPDGYPTYN FAVVVDDWDM GITEVIRGDD HINNTPRQIN LYEGIGAPVP KFGHMPMILD EQGAKLSKRT GAADVMQYKD AGYLPDALLS YLARLGWSHG DQELFSRQEL IELFDVKDCN SKASRLDMAK LGWVNQHFLK TEDVAAIVPH LVYQLQKLGL DVAAGPAPED VVVALRERVQ TLKEMAEKAV VWYQPLTEYD EAAVAKHFKA GAEVALGKAR ELLAALPEWT AESVGVALHD AAAALEIGMG KVAQPLRVAI TGTQVSPDIS HTVYLAGREQ ALKRIDVAIT KVATA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCT GCCGCACCCG CTTCGCCCCC AGTCCCACCG GCTACCTGCA CATCGGTGGT GCCCGCACTG CGCTGTACTG CTGGCTGGAG GCCCGCCACC GTGGCGGCGA GTTCGTGCTG CGTATCGAGG ACACCGACCG TGAGCGCAGC ACCCAGGGCG CGATCGACGC CATCCTGGAG GCGATGGAGT GGCTGGGCCT GGACTATGAC GAAGGCCCGA TCTACCAGAC CGACCGCGTC GCCCGTTACC TGGAAGTGGC CGAACAGCTG GTGGCCGACG GCAAGGCGTA TTACGCATAC GAGACCCGCG AAGAGCTGGA CGCGATGCGC GAGGCCGCCA TGGCCAGGCA GGAAAAGCCG CGCTACAACG GAGCCGCGCG TGACCTGGGC CTGCCGCGCA GGGATGATCC GAACCGCGTC ATCCGCTTCA AGAACCCGCT GGAAGGTACG GTGGTGTTCG ACGACCTGAT CAAGGGCCGC ATCGAGATCG CCAACAGCGA GCTGGATGAC ATGGTCATCT TCCGCCCGGA CGGCTACCCC ACCTACAACT TCGCGGTGGT GGTGGACGAC TGGGACATGG GCATCACCGA GGTCATCCGC GGCGACGACC ACATCAACAA CACCCCGCGC CAGATCAACC TGTACGAAGG CATCGGTGCG CCTGTGCCGA AGTTCGGCCA CATGCCGATG ATCCTGGACG AGCAGGGCGC CAAGCTGTCC AAGCGCACCG GCGCGGCCGA CGTGATGCAG TACAAGGACG CCGGCTACCT GCCCGATGCG CTGCTGAGCT ACCTGGCCCG CCTGGGCTGG TCGCACGGTG ACCAGGAGCT GTTCAGCCGC CAGGAGCTGA TCGAGCTGTT CGACGTGAAG GACTGCAATT CCAAGGCCTC GCGCCTGGAC ATGGCCAAGC TGGGCTGGGT CAACCAGCAC TTCCTGAAGA CCGAGGACGT GGCCGCGATC GTGCCGCACC TGGTCTACCA GCTGCAGAAG CTGGGCCTGG ACGTGGCCGC TGGCCCGGCC CCGGAGGACG TGGTGGTCGC CCTGCGTGAA CGCGTGCAGA CCCTGAAGGA AATGGCCGAG AAGGCCGTGG TCTGGTACCA GCCGCTGACC GAGTACGACG AAGCGGCGGT AGCCAAGCAC TTCAAGGCCG GTGCCGAGGT GGCGCTGGGC AAGGCCCGCG AGCTGCTGGC CGCCCTGCCG GAATGGACCG CCGAGTCGGT GGGCGTCGCC CTGCACGACG CGGCCGCAGC GCTGGAAATC GGCATGGGCA AGGTCGCCCA GCCGCTGCGC GTGGCCATCA CCGGCACCCA GGTCAGTCCT GACATTTCCC ATACCGTGTA CCTGGCCGGC CGCGAGCAGG CCTTGAAACG CATTGATGTG GCCATCACCA AGGTAGCAAC GGCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT